Marker-based information on taxonomic and genetic variation in

Fagus sylvatica sensu lato

Dušan Gömöry & Ladislav Paule

Technical University in Zvolen, Zvolen, Slovakia

Complex Fagus sylvatica L. (Flora Europaea, Greuter & Burdet 1981):

Fagus sylvatica ssp. sylvatica Europe

Fagus sylvatica ssp. orientalis (Fagus orientalis Lipsky) Thrakia, Ponthic Mts., Amanus Mts., Great Caucasus, Small Caucasus, Alborz Mts. (F. hohenackeriana Palibin?)? Fagus moesiaca (Maly) Czeczott Serbia, Macedonia, Bosnia?, Bulgaria? Greece?? Fagus taurica Popl. Crimea

Gömöry et al. (2007) Bot J Linn Soc in press

Differentiation sylvatica vs. orientalis

Bayesian analysis of gene admixture12 allozyme loci, 279 populations

0

0,5

1

0 20 40 60

longitude [degrees]

pro

po

rtio

n o

f th

e 'o

rie

nta

lis

' g

en

e p

oo

l

subsp. sylvatica

subsp. sylvatica Calabria

subsp. sylvatica Balkans

subsp. orientalis Crimea

subsp. orientalis

regression

0

0,5

1

0 20 40 60

longitude [degrees]

pro

po

rtio

n o

f th

e '

ori

en

tali

s'

ge

ne

po

ol

subsp. sylvatica

subsp. sylvatica Calabria

subsp. sylvatica Balkans

subsp. orientalis Crimea

subsp. orientalis

regression

Morphometry, 9 leaf and fruit traits

Denk et al. (2002) Plant Syst Evol 232: 213–236

Gömöry et al. (2007) Bot J Linn Soc in press12 allozyme loci, 202 populations

Denk et al. (2002) Plant Syst Evol 232: 213–236

nrDNA ITS

Phylogeny of the Fagus sylvatica complex

syl F. sylvatica sylvaticasyl* SW Balkansori F. sylvatica orientalisori* Crimea

Fagus moesiaca ?12 allozyme loci

Gömöry et al. (1999) J Evol Biol 12: 746-754

Mdh-C

s

s

s

s

s

s

m

mm

mo

s

Comps/Gömöry in Magri et al. (2006) New Phytologist 171: 199–221

Fagus sylvatica

7 allozyme loci, 608 populations, SAMOVAcpSSRcpDNA PCR-RFLP

Vendramin in Magri et al. (2006) New Phytologist 171: 199–221Vettori et al. (2004) Theor Appl Genet 109: 1–9Geburek in Magri et al. (2006) New Phytologist 171: 199–221

Magri et al. (2006) New Phytologist 171: 199–221

Postglacial migration (FOSSILVA)

Projection of SAMOVA groups ontopaleobotanic sites

▲ macrofossils● C14 dated pollen > 2%

Comps et al. (2001) Genetics 157: 389–397

Genetic footprints of postglacial colonization

Caveats for the interpretation of theprovenance trial

• taxonomic differentiation within the range (Gramatikovo!, Balkan provenances)

• origin from different refugia (Central Europe vs. Italy (vs. S France?) – different initial gene pool

• lower initial allelic richness in northern (western?) marginal populations