maria dinescu - ifa · nilprp (national institute for lasers, plasma and radiation physics)...
TRANSCRIPT
Maria Dinescu
National Institute for Lasers Plasma and Radiation Physics
(NILPRP)
httpppaminflprro
NILPRP (National Institute for Lasers Plasma and Radiation Physics) Bucharest Romania
In the top position in the country as importance (dimension and scientific contribution)
Main field lasers and plasma physics and applications
450 peoples 235 scientists 60 PhD students
Five departments Laser Department (PPAM)
Laboratory of Solid State and Quantum Electronics
Plasma Physics and Nuclear Fusion Laboratory
Low Temperature Plasma Laboratory
Accelerators Laboratory
Laser Metrology
Involved in national and international (EURATOM FP7 NATO SfP EUREKA etc) projects
NILPRPPHOTONIC PROCESSING OF ADVANCED MATERIALS
Group (PPAM)
httpppaminflprro
The group was organized starting with 1996 and in present contains 17 qualified scientists and 2 technicians
Topic
The activity is focused on laser processing of matter with applications in thin films and nanostructures with
functional properties functional polymers protein and cell transfer for tissue engineering chemical sensors for the
detection of warfare agents
Expertise
Thin films and heterostructures obtained by PLD and RF-PLD for different electronic applications
-Ferroelectrics piezoelectrics and relaxors for electronic microwave and optoelectronic applications titanates (PZT
La doped PZT BTO BST etc) niobites ( SBN PMN NKN) tantalates (SBT BZT NBT)
-Zinc oxide (ZnO) piezoelectric n-type semiconductor p-type semiconductor- ZnOMgxZn1-xO and
MgxZn1-x ZnO MgxZn1-x
-III-V compounds AlN InN GaN and their combinations
-Heterostructures PMNLSCO PZTTiN CNSiCNSiC SBNSTON
-High-k dielectric materials ZrO2 ZrSixOy HfO2 HfSixOy Nb2O5 NbSixOy Ta2O5 TaSixOy
-Wide band gap semiconductor metallic oxide WOx
Nanomaterials for catalytic and biological applications
- catalytic systems and porous materials fabrication by laser and conventional techniques
- nanomaterials for drug delivery
International Projects 1999-2011
Romanian Coordinator of FP 7 FP7-ICT-2009-4-247868 e-LIFT ldquoLaser printing oforganicinorganic material for the fabrication of electronic devicesrdquo project (2010-2012)
NATO-SfP Project Co-Director 982671 project Polymers based piezoelectric sensor array forchemical warfare agents detection (2007-2011)
Romanian Coordinator of FP 6 NMP3-CT-2006-033297 3D-DEMO Single step 3D Deposition ofcomplex nanopatterned Multifunctional Oxides thin films project (2006-2010) Priority 3ndash NMP research area 3422-2 ldquoMultifunctional ceramic thin films with radically newpropertiesrdquo
Romanian Coordinator of FP 5 IST ndash2001-33326 ldquoPiezoelectric sensor arrays forbiomolecular interactions and gas monitoringrdquo (PISARRO) project (2002-2004)
NATO Linkage grant Growth of Ferroelectric Thin Films by fs Pulsed Laser Deposition(2003-2005)
NATO SfP Co-Director of the Project 97-1934 ldquo Laser Based Clean Technologies for SmartSensor Applicationsrdquo (1999-2002)
RF Assisted Pulsed Laser Deposition Experimental set-up
Fundamentals of the MAPLE process
Substrate
Laser
light
Solvent
molecules
pumped away
Frozen
target
Thin film
of
polymer
protein
Target
holder
frozen
The solvent and the solute concentration are chosen that
bull the solute can be dissolved without formation of clusters
bull no chemical or photochemical reactions between the solvent and
solute
Most of the laser energy is absorbed by the volatile matrix
bull photochemical decomposition can be minimized
X-Y-Z processing system
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
NILPRP (National Institute for Lasers Plasma and Radiation Physics) Bucharest Romania
In the top position in the country as importance (dimension and scientific contribution)
Main field lasers and plasma physics and applications
450 peoples 235 scientists 60 PhD students
Five departments Laser Department (PPAM)
Laboratory of Solid State and Quantum Electronics
Plasma Physics and Nuclear Fusion Laboratory
Low Temperature Plasma Laboratory
Accelerators Laboratory
Laser Metrology
Involved in national and international (EURATOM FP7 NATO SfP EUREKA etc) projects
NILPRPPHOTONIC PROCESSING OF ADVANCED MATERIALS
Group (PPAM)
httpppaminflprro
The group was organized starting with 1996 and in present contains 17 qualified scientists and 2 technicians
Topic
The activity is focused on laser processing of matter with applications in thin films and nanostructures with
functional properties functional polymers protein and cell transfer for tissue engineering chemical sensors for the
detection of warfare agents
Expertise
Thin films and heterostructures obtained by PLD and RF-PLD for different electronic applications
-Ferroelectrics piezoelectrics and relaxors for electronic microwave and optoelectronic applications titanates (PZT
La doped PZT BTO BST etc) niobites ( SBN PMN NKN) tantalates (SBT BZT NBT)
-Zinc oxide (ZnO) piezoelectric n-type semiconductor p-type semiconductor- ZnOMgxZn1-xO and
MgxZn1-x ZnO MgxZn1-x
-III-V compounds AlN InN GaN and their combinations
-Heterostructures PMNLSCO PZTTiN CNSiCNSiC SBNSTON
-High-k dielectric materials ZrO2 ZrSixOy HfO2 HfSixOy Nb2O5 NbSixOy Ta2O5 TaSixOy
-Wide band gap semiconductor metallic oxide WOx
Nanomaterials for catalytic and biological applications
- catalytic systems and porous materials fabrication by laser and conventional techniques
- nanomaterials for drug delivery
International Projects 1999-2011
Romanian Coordinator of FP 7 FP7-ICT-2009-4-247868 e-LIFT ldquoLaser printing oforganicinorganic material for the fabrication of electronic devicesrdquo project (2010-2012)
NATO-SfP Project Co-Director 982671 project Polymers based piezoelectric sensor array forchemical warfare agents detection (2007-2011)
Romanian Coordinator of FP 6 NMP3-CT-2006-033297 3D-DEMO Single step 3D Deposition ofcomplex nanopatterned Multifunctional Oxides thin films project (2006-2010) Priority 3ndash NMP research area 3422-2 ldquoMultifunctional ceramic thin films with radically newpropertiesrdquo
Romanian Coordinator of FP 5 IST ndash2001-33326 ldquoPiezoelectric sensor arrays forbiomolecular interactions and gas monitoringrdquo (PISARRO) project (2002-2004)
NATO Linkage grant Growth of Ferroelectric Thin Films by fs Pulsed Laser Deposition(2003-2005)
NATO SfP Co-Director of the Project 97-1934 ldquo Laser Based Clean Technologies for SmartSensor Applicationsrdquo (1999-2002)
RF Assisted Pulsed Laser Deposition Experimental set-up
Fundamentals of the MAPLE process
Substrate
Laser
light
Solvent
molecules
pumped away
Frozen
target
Thin film
of
polymer
protein
Target
holder
frozen
The solvent and the solute concentration are chosen that
bull the solute can be dissolved without formation of clusters
bull no chemical or photochemical reactions between the solvent and
solute
Most of the laser energy is absorbed by the volatile matrix
bull photochemical decomposition can be minimized
X-Y-Z processing system
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
NILPRPPHOTONIC PROCESSING OF ADVANCED MATERIALS
Group (PPAM)
httpppaminflprro
The group was organized starting with 1996 and in present contains 17 qualified scientists and 2 technicians
Topic
The activity is focused on laser processing of matter with applications in thin films and nanostructures with
functional properties functional polymers protein and cell transfer for tissue engineering chemical sensors for the
detection of warfare agents
Expertise
Thin films and heterostructures obtained by PLD and RF-PLD for different electronic applications
-Ferroelectrics piezoelectrics and relaxors for electronic microwave and optoelectronic applications titanates (PZT
La doped PZT BTO BST etc) niobites ( SBN PMN NKN) tantalates (SBT BZT NBT)
-Zinc oxide (ZnO) piezoelectric n-type semiconductor p-type semiconductor- ZnOMgxZn1-xO and
MgxZn1-x ZnO MgxZn1-x
-III-V compounds AlN InN GaN and their combinations
-Heterostructures PMNLSCO PZTTiN CNSiCNSiC SBNSTON
-High-k dielectric materials ZrO2 ZrSixOy HfO2 HfSixOy Nb2O5 NbSixOy Ta2O5 TaSixOy
-Wide band gap semiconductor metallic oxide WOx
Nanomaterials for catalytic and biological applications
- catalytic systems and porous materials fabrication by laser and conventional techniques
- nanomaterials for drug delivery
International Projects 1999-2011
Romanian Coordinator of FP 7 FP7-ICT-2009-4-247868 e-LIFT ldquoLaser printing oforganicinorganic material for the fabrication of electronic devicesrdquo project (2010-2012)
NATO-SfP Project Co-Director 982671 project Polymers based piezoelectric sensor array forchemical warfare agents detection (2007-2011)
Romanian Coordinator of FP 6 NMP3-CT-2006-033297 3D-DEMO Single step 3D Deposition ofcomplex nanopatterned Multifunctional Oxides thin films project (2006-2010) Priority 3ndash NMP research area 3422-2 ldquoMultifunctional ceramic thin films with radically newpropertiesrdquo
Romanian Coordinator of FP 5 IST ndash2001-33326 ldquoPiezoelectric sensor arrays forbiomolecular interactions and gas monitoringrdquo (PISARRO) project (2002-2004)
NATO Linkage grant Growth of Ferroelectric Thin Films by fs Pulsed Laser Deposition(2003-2005)
NATO SfP Co-Director of the Project 97-1934 ldquo Laser Based Clean Technologies for SmartSensor Applicationsrdquo (1999-2002)
RF Assisted Pulsed Laser Deposition Experimental set-up
Fundamentals of the MAPLE process
Substrate
Laser
light
Solvent
molecules
pumped away
Frozen
target
Thin film
of
polymer
protein
Target
holder
frozen
The solvent and the solute concentration are chosen that
bull the solute can be dissolved without formation of clusters
bull no chemical or photochemical reactions between the solvent and
solute
Most of the laser energy is absorbed by the volatile matrix
bull photochemical decomposition can be minimized
X-Y-Z processing system
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
International Projects 1999-2011
Romanian Coordinator of FP 7 FP7-ICT-2009-4-247868 e-LIFT ldquoLaser printing oforganicinorganic material for the fabrication of electronic devicesrdquo project (2010-2012)
NATO-SfP Project Co-Director 982671 project Polymers based piezoelectric sensor array forchemical warfare agents detection (2007-2011)
Romanian Coordinator of FP 6 NMP3-CT-2006-033297 3D-DEMO Single step 3D Deposition ofcomplex nanopatterned Multifunctional Oxides thin films project (2006-2010) Priority 3ndash NMP research area 3422-2 ldquoMultifunctional ceramic thin films with radically newpropertiesrdquo
Romanian Coordinator of FP 5 IST ndash2001-33326 ldquoPiezoelectric sensor arrays forbiomolecular interactions and gas monitoringrdquo (PISARRO) project (2002-2004)
NATO Linkage grant Growth of Ferroelectric Thin Films by fs Pulsed Laser Deposition(2003-2005)
NATO SfP Co-Director of the Project 97-1934 ldquo Laser Based Clean Technologies for SmartSensor Applicationsrdquo (1999-2002)
RF Assisted Pulsed Laser Deposition Experimental set-up
Fundamentals of the MAPLE process
Substrate
Laser
light
Solvent
molecules
pumped away
Frozen
target
Thin film
of
polymer
protein
Target
holder
frozen
The solvent and the solute concentration are chosen that
bull the solute can be dissolved without formation of clusters
bull no chemical or photochemical reactions between the solvent and
solute
Most of the laser energy is absorbed by the volatile matrix
bull photochemical decomposition can be minimized
X-Y-Z processing system
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
RF Assisted Pulsed Laser Deposition Experimental set-up
Fundamentals of the MAPLE process
Substrate
Laser
light
Solvent
molecules
pumped away
Frozen
target
Thin film
of
polymer
protein
Target
holder
frozen
The solvent and the solute concentration are chosen that
bull the solute can be dissolved without formation of clusters
bull no chemical or photochemical reactions between the solvent and
solute
Most of the laser energy is absorbed by the volatile matrix
bull photochemical decomposition can be minimized
X-Y-Z processing system
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Fundamentals of the MAPLE process
Substrate
Laser
light
Solvent
molecules
pumped away
Frozen
target
Thin film
of
polymer
protein
Target
holder
frozen
The solvent and the solute concentration are chosen that
bull the solute can be dissolved without formation of clusters
bull no chemical or photochemical reactions between the solvent and
solute
Most of the laser energy is absorbed by the volatile matrix
bull photochemical decomposition can be minimized
X-Y-Z processing system
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
X-Y-Z processing system
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
X-Y-Z processing system
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
PPAM Processing Laboratory
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Processing Equipments
12 cm
PLD and RF-PLD deposition systems
x-y-z laser processing system (LIFT)
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Optical analysis
Spectroellipsometer
PHOTONIC PROCESSING OF ADVANCED MATERIALS Group (PPAM)
Characterization Equipments
AFM
Morphological analysis
XRD
Structural analysis
SIMS
Chemical analysisDielectric and ferroelectric analysis
Impedance analyzer
NILPRP
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Other equipments-INFLPR
SEM (Scanning Electron Microscope)
X-Ray diffractometer for powders with temperature chamber
FTIR (Fourier Transform Infrared Spectroscopy)
Contact Angle Measurements
Plasma Spectroscopy
hellip
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Laser Induced Forward Transfer (LIFT)
Laser light is focused on
the target interface
An expelling process
takes place
Ejected material is deposited
on the receiving substrate
bull Precise and high density patterns
bull High spatial resolution
bull Contact or Non-contact rapid automated method
bull Flexibility as working distances target material size of the transferred droplets
bull No significant damage to transferred material under specific conditions hellip
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Target
Advantages
bullAvoid direct irradiation of sensitive
material
bullAfter laser radiation large amount
of gaseous products that acts as
carrier for larger ablation products
TRIAZENE POLYMER
OUR APPROACH LIFT using a sacrificial release layer (TP)
(Nagel et al Macromolecular Chemistry and Physics 2007)
Polymer
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Printing on sensor matrices
Polymer λ Ф
[mJcm2]
Bck
pressure
[mbar]
Film
thickne
ss [nm]
PIB 266 nm 02 ~ 10-4 ~ 60
PEI 04
PECH 06
XeCl 308 nm 1 Hz Ф=400-500 mJcm2 TP 100nm
PEIPIB PEI PECH
400 microm 400 microm 400 microm
DONOR DONORDONOR
RECEIVER
15
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Frequency response against time of PEI PIB and PECH
polymers to different concentrations of DMMP
The frequency shift normalized to the central frequency
(about 392 MHZ)
Sensor array responses to simulant DMMP and EA
2-port SAW resonators operating at 392 MHz
The interdigital transducers were shaped
with a Gaussian apotization with a wavelength
of 8 and fingers overlap of 450microm while the cavity
length was 1276 microm
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
The testing setup of SAW sensors with sarin gas
17
Sensor array responses to sarin nerve agent
Tests carried aut at CBRN military base (Bucharest)
Time response of a PIB coated sensor to
46 ppm of Sarin
178 ppm of Sarin
Response curve for PEI PIB and PECH
coated SAW sensors to
different concentrations of Sarin
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
NOT limited to polymer materials
Examples of various materials
Liposomes A Palla-Papavlu et al Appl Phys A (2011)
18
Polystyrene microbeads A Palla-Papavlu et
al JAP (2010)
Mammalian cells
Doraiswamy et al ApplSurf Sci(2006)
Quantum dots Xu et al
Nanotech (2007)
Functional OLEDs R Fardel et al
Appl Phys Lett (2007)
Al R Fardel et al
Appl Surf Sci (2007)
GdGaO Banks et al (2008)
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
RECEIVER
Laser fluence mJcm2
550 500 450 400 350
DONOR
KEY PATTERNING PARAMETERS
Laser fluence ndash 350 ndash 550 mJcm2
Thickness ratio PEI to TP ndash 150 nm TP100 nm PEI
(PEI thickness gt TP thickness)
Transfer distance ndash contact
Minimum thickness of the TP layer ndash 100 nm
Furthermore Polymer micropatterning for cellular behavior studies
MATERIALS1048707 Polyethilene glycol (PEG)-Repellent for cells1048707 Polyethileneimine (PEI)-attachment vector1048707 Growth medium 10 FCS and 01
penicilinsteptomicin1048707 Trypsyn-EDTA solution 025 and 002 in PBS1048707 DMEM with phenol red1048707 SH-SY5Y human neuroblastoma cells cultured in
FCS-DMEM
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al APA 2011
20 microm 20 microm
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Other polymers Polystyrene microbeads
Microfabrication of polystyrene microbead arrays by laser induced forward transfer A Palla-Papavlu et al JAP 2010
(PS-microbead) microarraysApplicationsBiosensingBioseparationBiomolecule screening
ExperimentalDonor PS-microbeads (size 8 microm) Receiver Thermanox coverslipsPatterning system XeCl 308 nm 30ns 1HzFluence 80 mJcm2 ndash 35 Jcm2
100 nm thick TP film as DRL
Scale bar is 100 microm
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
a) PEI array in cell medium
b) SH-SY5Y distribution on patterned surfaces after one hour
c) After one day in vitro A clear clustering of neurons into aggregates is visible on the PEI pixel
a) PEI array in cell culture medium
b) Neural cells attached on the substrate after 3 days in vitro (scale bar is 200 microm)
Polymer micropatterning for cellular behavior studies Parameters optimization
Polymer spatially controlled micro patterning for cellular behavior study V Dinca et al submitted 2010
Average number of observed interconnecting neurite fascicles vs the separation distance between the transferred PEI-pixels
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
Liquid printing
Liquid printing
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
water + glycerol (5050) scale bar is 40 microm
1 Influence of DRL thickness ndash 60 nm 150 nm and 350 nm
2 Laser fluence
3 Different glycerol concentrations (10 ndash 70 )
4 Time resolved imaging for 308 nm and 193 nm
ndash laser fluences and glycerol concentration
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
International Cooperations
PSI Villigen ndashThomas Lippert (ETH Zurich)
ldquoOMCorbinordquo Institute for Acoustics and Sensors-CNR Italy
Naval Research Laboratory USA
FORTH-IESL Crete
httpppaminflprro
httpppaminflprro