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    NOTICE

    THE MATERIAL IN THIS MANUAL IS FOR INFORMATIONPURPOSES ONLY. THE CONTENTS AND THE PRODUCT ITDESCRIBES ARE SUBJECT TO CHANGE WITHHOUT NOTICE.NAPCO MAKES NO REPRESENTATIONS OR WARRANTIES WITHRESPECT TO THIS MANUAL. IN NO EVENT SHALL NAPCO BELIABLE FOR ANY DAMAGES, DIRECT OR INCIDENTAL, ARISINGOUT OF OR RELATED TO THE USE OF THIS MANUAL.

    For repair information or replacement parts assistance from the manufacturer,call Customer Service using our toll free telephone number.

    800-621-8820540-869-9892

    (FAX) 540-869-0130

    THIS SYMBOL INDICATES HAZARDOUS VOLTAGES MAY BE PRESENT.

    THIS SYMBOL MARKS CHAPTERS AND SECTIONS OF THIS INSTRUCTION

    MANUAL WHICH ARE PARTICULARLY RELEVANT TO SAFETY.WHEN ATTACHED TO THE UNIT, THIS SYMBOL DRAWS ATTENTIONTO THE RELEVANT SECTION OF THE INSTRUCTION MANUAL.

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    INDEX DATE

    REVISION STATUS

    Initial release A 6/99

    AMENDED PAGES NOTES

    Update parts list for new kit no's.B 11/99Update for coved cornersC 5/02 29-31

    Update parts listD 8/04 30 Add stacking instructionsE 12/04 5, 37

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    1

    1. 0 Introduction

    1.01 Your satisfaction and safety are important toNAPCO and a complete understanding of this unit is necessary to attain theseobjectives.

    1.02 As the ultimate user of this apparatus, youhave the responsibility to understand its proper function and operational characteristics. Thisinstruction manual should be thoroughly readand all operators given adequate trainingbefore attempting to place this unit in service.

    Awareness of the stated cautions andwarnings, and compliance withrecommended operating parameters together with maintenance requirements are important for safe and satisfactoryoperation. The unit should be used for itsintended application; alterations or modifications will void the warranty.

    WARNING AS A ROUTINE LABORATORY PRECAUTION, AL WA YS WE AR SA FETY GL ASSE S WH EN WORKING WITH THIS APPARATUS.

    1.03 This product is not intended, nor can it beused, as a sterile or patient connected device.In addition, this apparatus is not designed for use in Class I, II, or III locations as defined bythe National Electrical Code.

    2.0 Unpacking and Damage

    2.01 This product was carefully packed andthoroughly inspected before leaving our factory. Save all packing material if apparatusis received damaged.

    2 .02 Responsibility for safe delivery was assumedby the carrier upon acceptance of theshipment; therefore, claims for loss or damage sustained in transit must be madeupon the carrier by the recipient as follows:

    Visible Loss or Damage : Note any externalevidence of loss or damage on the freight billor express receipt, and have it signed by thecarrier's agent. Failure to adequately describesuch external evidence of loss or damage

    may result in the carrier's refusing to honor your claim. The form required to file suchclaim will be supplied by the carrier.

    Concealed Loss or Damage : Concealedloss or damage is any loss or damage whichdoes not become apparent until themerchandise has been unpacked andinspected. Should either occur, make awritten request for inspection by carrier'sagent within 15 days of the delivery date; thenfile a claim with the carrier.

    2.03 If you follow the above instructions carefully,Precision Scientific will guarantee our fullsupport of your claim to be compensated for loss or damage in transit.

    DO NOT for any reason return thisunit to NAPCO without first obtainingreturn authorization. In anycorrespondence with NAPCO please supplythe nameplate data, including catalog number and serial number.

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    3.0 General and MaintenanceInformation

    3.01 Napco water jacketed, microprocessor controlled CO 2 incubators are ideal for applications requiring precise and uniformcontrol of temperature and CO

    2. They provide

    a controlled environment for the growth of culture common in virology, physiology andmicrobiology.

    3.02 The solid state digital control panel houses allfunctions necessary to operate the incubator.The push button switches and individual LEDdisplays allow the operator to adjusttemperature, CO 2, and calibration via a singleset of controls. No adjusting of trim pots isrequired for operation.

    3.03 The proportional integral derivative (PID)temperature control allows precisetemperature control from 5 C above ambientto 50 C. The patented internal air heater located within the chamber provides rapidtemperature recovery unparalleled in theindustry.

    3.04 A digital hi-limit safety switch is providedwhich can be easily set through the controlpanel to prevent thermal runaway in the eventof temperature control failure.

    3.05 Visible and audible Hi and Lo alarms areprovided for temperature and CO 2. Contactsare also provided on the rear of the unit for connection to a central monitoring system.

    3.06 Your incubator is supplied with five shelvesper chamber and can hold a maximum of tenshelves per chamber. The entire stainlesssteel shelf system can be assembled anddisassembled without the use of tools for ease of cleaning.

    3.07 A stainless steel humidity pan is also suppliedwith the unit to promote high RH levels withinthe chamber. The humidity pan rests on thechamber floor to promote quick recovery of RH. For even quicker RH recovery levels, thebottom of the chamber may be flooded withdistilled water.

    3.08 The incubators are available in both singlechamber and double chamber configurations.The double units contain two independentsets of controls enabling operation of a singlechamber even when the other is turned off.

    3.09 Maintenance: Add water to the water jacketwhen the red low water light is illuminated.Refer to Section 8.06. Bimonthly calibrationof the unit is recommended. See section10.0. For cleaning, see Section 12.0. Normaloperation requires periodic filling of thehumidity pan. See section 10.01. No other maintenance is required.

    3.10 As always, the NAPCO support team is ready

    to help.Please call 540-869-9892 if you have anyquestions or require further assistance.

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    3

    4.0 Specifications

    4.01 The following table lists the performance specifications for these CO 2 incubators:

    "R" suffix added to model number denotes right-hinged door

    Environmental ConditionsIndoor Use OnlyMaximum Altitude 2000 metersOperating Ambient: 5 to 40 Relative Humidity: 80% for temperatures to 31

    50% for temperatures to 40

    Pollution Degree: 2

    TABLE 4.1 Specifications

    Single Chamber

    CatalogNumber 115

    VAC

    TC Sensor

    51200063

    IR Sensor

    51200064

    Chamber Volume:

    5.4 cu. ft.153.5 liters

    Chamber Dimensions:(usable)

    (L x W x H)17.3 x 17.5x 25 in.

    439 x 445 x 635 mm

    Exterior Dimensions:

    (L x W x H)27.8 x 24.5 x 33.8 in.706 x 622 x 859 mm

    Temperature

    Range:Control:Stability:Uniformity:

    Ambient +5.0C to 50.0C0.1C

    0.1C0.25C

    Ambient + 5.0C to50.0C

    CO2Range:Control:Stability:Uniformity:

    0 to 20.0%0.1%

    0.1%0.25%

    0 - 20.0%

    Humidity Range: Above ambient to 98%

    Humidity Source: 1 Pan (supplied)

    Shelves: 5 (supplied)10 (maximum)

    Shelf Capacity:(maximum)

    20 sq. ft.1.84 sq. meter

    Electrical ServiceOvervoltage Category II+/-10% 50/60Hz

    115V~ 550 Watts 4.7 Amps

    Maximum BTU Output: 1876

    Net Weight: 188 lbs., 85 kg

    ShippingInformationDimensions:Weight:Volume:

    L x W x H36 x 33 x 40 in.

    915 x 838 x 1016 mm265 lbs., 120 kg

    29.5 cu. ft., 0.83 cu. meter

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    6.01.2 Front Panel Displays

    "Temp. C": Displays actual chamber temperature duringoperation. May be used for other display

    purposes during setup and calibration.

    "% CO 2": Displays actual chamber CO 2 during operation.May be used for other display purposes

    during setup and calibration.

    6.01.3 Front Panel Fittings and Connectors

    "Fill Port:" Used to fill or to siphon drain the chamber water jacket.

    "CO 2Sample:" Provides a direct connection for sampling the

    chamber atmosphere for calibration of the CO 2displayed value.

    6.01.4 Front Panel Indicator Lamps

    "H2O" Indicates that water in the water jacket isbelow normal operating level.

    "Lock" Illuminates when control panel settings havebeen locked.

    "High" Indicates that the current displayed value isabove SETPOINT.

    "Low" Indicates that the current displayed value isbelow SETPOINT.

    "On" Indicates that controller is regulating thisparameter.

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    6.3 Amp

    "CO2Inlet:" Provides 1/4" I.D. tubing connectors for connection

    to one CO 2 cylinder (tank). There is one set of CO 2input connectors per incubator.

    6.02 Rear Panel

    6.03 Left Side Panel

    Fuses: The (2) fuses provides protection for the primaryincubator power line. Fuse current rating varies,depending on supply voltage.

    "Power:" Primary electrical power switch. There is one

    power switch for each chamber.

    "ACInput:" IEC standard connector for connection to electrical

    service line. There is one "AC input" per incubator.

    "TempRecorder:" Used to connect a recording device (10mv DC/C)

    to monitor chamber temperature if desired. "+" and"-" connectors indicate signal polarity. There is onerecorder output per chamber.

    "CentralMonitoringSystem (C.M.S)

    AlarmOutput:" Used to connect to a central monitoring system if

    desired. Connection is Form "C" contacts. May bewired NC (Normally Closed) or "NO" (NormallyOpen) with isolated ground ("COM" connection).

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    7.0 Cleaning andDecontamination

    WARNINGDISCONNECT POWER BEFORE PROCEEDING.SOME PARTS MAY BE HOT.

    NOTETHIS INCUBATOR IS MANUFACTURED IN AN INDUSTRIAL ENVIRONMENT. WHILE EVERY EFFORT IS MADE TO KEEP THIS INCUBATOR AS CLEAN ASPOSSIBLE DURING MANUFACTURE AND TRANSIT,IT IS NOT STERILE.

    7.01 To ensure optimal growth conditions, westrongly recommend you thoroughly cleanand disinfect the incubator prior to use.

    7.02 Open the incubator doors and remove anypackaging or accessory items.

    7.03 Remove the air duct from each chamber

    1. Loosen nut.

    2. Carefully tilt top of air duct forward and towardeither side.

    3. Remove air duct from chamber.7.04 Thoroughly clean and disinfect chamber(s),

    air duct(s), shelves, supports, shelf slidebrackets, shelf slides, humidity pan(s), glassdoor, gasket, latch, and any other objectswhich will be placed inside the chamber. Allstainless steel parts may be autoclaved for

    thorough sterilization.

    7.05 Blower wheel may be removed and cleaned.The blower wheel is fastened to the motor axle by a firm press fit. Some force may berequired for removal.

    NOTEHANDLE ALL STERILIZED PARTS CAREFULLY TOREDUCE POSSIBILITY OF INTRODUCINGCONTAMINANTS INTO THE INCUBATOR .

    7.06 Re-install blower wheel and air duct.

    Press the blower wheel firmly onto the axleuntil the blower wheel rests against the axlestop. Place the plastic spacer on the longstud located on the fan motor plate. Re-installthe air duct and fasten the plastic nut. Checkfor free rotation of the blower wheel by turningthe wheel clockwise using a finger. If theblower wheel rubs against the air duct: a)verify that wheel is pushed back against theaxle stop, and b) loosen the nut slightly.

    Nut

    Air Duct

    Blower Wheel

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    9

    8.0 Set-up

    8.01 Ensure that the incubator is level. Adjust the4 corner leveling feet as needed.

    8.02 Ensure that the incubator is plugged into aproperly grounded outlet of the correct supplyvoltage, and that the power switch is in the off position (0).

    8.03 Ensure that the blower wheel and air duct areproperly installed (See Section 7.06).

    8.04 Install front shelf slide supports by slidingthe keyed slots of each support into placeover the buttons located near the top andbottom of each side wall of the chamber. Notethat the keyed mounting slots should be at the

    edge of the shelf slide support which facesthe front of the chamber.

    8.05 Install shelves . The incubator is providedwith five (5) shelves per chamber, and willaccommodate up to ten (10) shelves per chamber. Each shelf rides on a pair of shelf slides for ease of access to samples. Shelf height may be adjusted by installing the slidesinto the proper keyways in the shelf slidesupports. Additional shelf kits (which includeone (1) shelf and one (1) pair of shelf slides)are available through your laboratoryequipment dealer or from NAPCO.

    Note that the shelf slides are keyed differentlyat each end the end which installs into therear slide support bracket has a key which isparallel to the length of the slide, while the endwhich installs into the front slide supportbracket has a key which is perpendicular tothe length of the slide.

    1. Refer to the following illustration and insertthe shelf slide into a rear keyway of your choice in the rear shelf slide bracket on either side of the chamber. Next insert the shelf slide into the matching front keyway. Attachanother slide on the opposite side of thechamber at the same elevation. Repeat for

    the remaining shelf slides.

    2. Slide a shelf into each pair of shelf slides. Theshelves are designed so that they may beused with the lip facing either up or down.

    8.06 Fill each chamber's water jacket with distilledwater(approximately 11 gallons [40 Liters]).

    WARNINGWHEN SETTING UP VERTICALLY STACKEDINCUBATORS, THE LOWER CHAMBER WATER JACKET MUST BE FILLED FIRST.

    CAUTIONTO AVOID BUILD-UP OF MINERAL DEPOSITS ANDTO PREVENT CORROSION, USE ONLY DISTILLEDWATER (50-K OHMS TO 1-M OHMS) IN THE WATER JACKET.

    DO NOT USE ULTRA-PURE OR DEIONIZED WATER.

    1. Turn the power switch on. The digital displayswill light, and the H 2O low water jacket water level indicator will be illuminated. Verify that allsetpoints are set to "OFF" or "0".

    Setpoints can be adjusted to "OFF" or "0" asfollows:

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    Press

    Press until

    or

    Press .

    Repeat for other parameters by pressing

    until the appropriate display is active.

    2. Unscrew the cap from the water jacket fill portand replace the cap with the threaded hosebarb connector provided in the accessorieskit.

    3. Connect one end of the clear vinyl tubingprovided in the accessory kit to the hose barbconnector.

    4. Pour one ounce of Quaternary Ammoniumgermicide (provided in accessory kit) into thewater jacket through the tubing to reducepossible fungus growth in the water jacket.

    5. Connect the other end of the vinyl tubing to asource of distilled water. A faucet adapter isprovided in the accessories kit for your convenience in connecting to an in-housecentral distilled water system.

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    11

    9.0 Connecting External Supplies

    9.01 Type of gasses required

    1. Only medical grade 100% CO 2 should beused as gas supply for this incubator.The gas source may be either a cylinder or an in-house central gas supplyequipped with shut-off valves and apressure regulator.

    WARNINGDO NOT USE CO 2 THAT HAS BEEN MIXED WITH OTHER GASSES OR AIR AS THIS WILL CAUSE POOR GAS CONTROL PERFORMANCE OR POSSIBLE HAZARDS.

    9.02 Gas Pressure Regulation

    9.02.1 When using cylinders as the gas supply,a 2-stage pressure regulator is requiredto reduce the tank pressure to the 15-25 PSI (1.0 - 1.7 BAR) recommendedoperating pressure. A 2-stage regulator must be used for each cylinder connected.

    9.02.2 When using an in-house central gassupply, either a 1-stage or 2-stagepressure regulator will be requireddepending on the pressure level of thein-house source. Check with your facilities personnel and with your regulator vendor to ensure that theregulator will provide adequatepressure control at the 15 - 25 PSI (1.0- 1.7 BAR) recommended operatingpressure. The regulator should beinstalled near the incubator.

    9.02 Gas Connection

    The accessory kit supplied with your incubator contains gas filters, hose clamps, and anample length of tubing for connecting theincubator with gas.

    Connect the gas supply to the incubator asfollows:

    1. Cut the supply hose to the lengthrequired.

    2. Slide two hose clamps over the hoseand connect one end of the hose to thepressure regulator and the other end tothe incubator. Tighten the hose clampsat each connection.

    CAUTIONDO NOT OVERTIGHTEN THE ADJUSTABLE HOSE CLAMPS. OVERTIGHTENING MAY DAMAGE THE HOSE.

    3. Cut through the gas supply hoseapproximately 1-2 feet from theincubator gas inlet. Place one hoseclamp over both open ends of the gastubing.

    NOTEOBSERVE GAS FLOW DIRECTION ON THE CO 2 FILTER.

    4. Connect a gas filter to the gas supplyhose.Tighten the hose clamps to the gasfilter.

    5. Repeat the above for each gas supplyused.

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    10.0Initial Operation & Calibration

    Successful operation of the incubator requirescareful attention to the calibration procedureslisted in this section. Failure to follow thecalibration procedures will result in poor operation of the incubator. This section shouldbe repeated anytime the incubator has beenout of service for more than 24 hours or whenpoor performance is suspected. Thecalibration procedure requires a minimumstabilization period of 24 hours.

    10.01 Fill the humidity pan (provided in theaccessories kit) with distilled water one inchdeep, and place pan on the floor of thechamber, at least two inches from the air

    duct.10.02 Place a reference thermometer on the center

    shelf of each chamber (Note: thermometersare not provided with the incubator). Thisthermometer will be used to verify temperaturecalibration. The thermometer should bepositioned such that it can be easily readthrough the incubator's inner glass door.

    WARNING

    WHEN USING MERCURY THERMOMETERS, HANDLE WITH THE UTMOST CARE. VERY SMALL AMOUNTSOF MERCURY FROM A BROKEN THERMOMETER MAY CAUSE DAMAGE TO THE CHAMBER BY ELECTROLYSIS. MERCURY ALSO CREATES A TOXIC ENVIRONMENT IN THE CHAMBER, RENDERING THE INCUBATOR USELESS.

    10.04 Enter Temperature Setpoint.

    1. Press

    2. Use and until display shows

    desired setpoint.

    3. Press .

    4. The incubator will begin heating asindicated by the green "ON" LED

    indicator to the left of the temperaturedisplay.

    CAUTIONNEVER OPERATE UNIT WITHOUT WATER IN THE WATER JACKET(S). OPERATION WITHOUT WATER IN THE WATER JACKET MAY RESULT IN POOR PERFORMANCE FROM THE INCUBATOR, OR MAY DAMAGE INCUBATOR COMPONENTS AND WILLVOID THE WARRANTY.

    10.05 Calibrate Temperature. Allow the incubator to operate for a minimum of 24 hours beforeattempting to calibrate temperature. Do notopen the glass door during this period.

    After the incubator has stabilized at the desiredoperating temperature (at least 24 hours),open the exterior door only. Do not openthe glass door . Compare the temperature of the digital display with the referencethermometer inside the chamber. If thesereadings match, no temperature calibration

    is required; therefore, skip to Section 10.06.If the readings do not match, continue readingthis section.

    NOTEDO NOT OPEN GLASS DOOR DURING THISPROCEDURE.

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    To calibrate temperature display:

    1. Press display will show

    2. Using and keys, match displayed

    temperature with reference thermometer reading.

    3. Press .

    Allow at least one hour for temperature tostabilize after calibration.

    After allowing incubator temperature tostabilize, verify that displayed temperaturematches the reference thermometer withinthe chamber. If not, repeat the above steps.

    10.06 Calibrate CO 2 to 0.

    Perform CO 2 calibration only after calibratingtemperature. Failure to calibrate Temp. prior to CO 2 will result in poor CO 2 performance.

    Do not be alarmed if the CO 2 display isnegative or far from zero. This is normal uponinitial calibration.

    10.07 Set CO 2.

    1. Press .

    2. Use or to set desired CO 2 level.

    3. Press .

    After the incubator has stabilized at operatingCO 2 level for at least 30 minutes, measure

    actual chamber CO 2 using a CO 2 gassampling device such as a FYRITE tester.

    10.08 Calibrate CO 2 to Measured Value.

    If the displayed CO 2 level does not match themeasured actual chamber CO 2 level, repeatthe CO 2 calibration by matching the displayedvalue to the actual measured value.

    1. Press twice. Display will show

    2. Using set CO 2 display to match reading

    on the sampling device.

    3. Press

    10.09 Check each calibration in the followingsequence and recalibrate as needed.

    1. Temperature2. CO 2

    Calibration is now completed.

    Relative humidity must be stable prior tocalibration of CO 2. Failure to do so will resultin poor CO 2 performance.

    1. Press twice. Display will show

    2. Using and set CO 2 display to 0.0%.

    3. Press .

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    11.03 Temperature Hi-Limit Safety

    1. Press :

    2. Use or until desired

    Hi-Limit safety is displayed.

    3. Press

    The temperature Hi-Limit safety has a rangefrom 26C to 57C. The temperature Hi-Limitsafety can not be set nearer than 0.5C abovetemperature setpoint.

    When the temperature setpoint is changedupward or downward, the Hi-limit safety willautomatically change a like amount to preventthe hi-limit safety from being set below thetemperature setpoint.

    11.04 Alarms

    This incubator provides both visible and audiblealarms to alert the operator to a variance in

    chamber conditions from SETPOINToperating conditions.

    11.04.1 "High" and "Low" Alarms

    Each controlled incubator function features"High" and "Low" alarms to indicate when theparameter's actual value rises above or fallsbelow a fixed band around setpoint. See Table11.1 for a listing of the alarm bands for eachparameter.

    If an alarm condition exists, the appropriatered "High" or Low" indicator lamp will light tothe left of the display and an audible alarmwill sound continuously. The alarms willremain active until the actual value returnsto within the normal operating setpoint band.

    11.0 Operation

    The Temperature and CO 2 functions have 3 modesof operation; "OFF", "0" and "Setpoint".

    OFF - When "OFF" is displayed in the window,the controlled function is inactive.

    0 - When "0" is displayed in the window, thecontrolled function only monitors anddisplays concentrations in the chamber.

    Setpoint - When a setpoint is entered, the unit willcontrol to the entered value.

    11.01 Changing Setpoints.

    1. Press until the desired setpoint

    window is active. The word will

    appear in one of the two windows.Dashed lines will appear in the non-active window. The selective parameter will be active.

    Temperature

    CO 2

    2. Press or to set desired

    setpoint.

    3. Press

    11.02 Calibration

    NOTETO ENSURE PROPER OPERATION OF THE INCUBATOR, CALIBRATION MUST BE COMPLETEDPER SECTION 10 IN THE FOLLOWING SEQUENCE:

    1. Temperature2. CO 2

    FAILURE TO DO SO MAY RESULT IN POOR INCUBATOR CONTROL.

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    Table 11.1 Alarm Bands and Delay Times

    Parameter AlarmBand

    AlarmDelay

    Default

    AlarmDelayLimits

    HIGH

    TEMP LOW

    +0.5C

    -0.5C

    5m

    10m

    0m-5m

    0m-20mm

    HIGHCO2 LOW

    +0.5%

    -0.5%

    5m

    10m

    0m-5m

    0m-20m

    11.04.2 Alarm Delays

    Each alarm has an associated delay toprevent nuisance alarms. The alarm delayshave been optimized and factory preset to

    prevent nuisance alarms under normaloperating conditions. If these alarm delaysettings are not appropriate for your application, they may be adjusted from thekeyboard.

    11.04.3 Changing Alarm Delays

    1. Press simultaneously,

    2 times. The incubator is now incommunication parameter set-up mode.

    0 will be displayed in the Temp.window and "CFG" will be displayed

    in the CO 2 window. Using the

    button, press and hold until "6" isdisplayed in the Temp. window.

    2. Press several times to cross

    through the communicationparameter set-up until the alarmdelays are reached.

    3. The di splay wi ll show HighTemperature Delay

    High

    4. Press or to change the

    temperature high alarm delay.

    5. Press to store the new value

    and cross to the next parameter.

    6. The di sp lay wi ll show LowTemperature Delay. Repeat step (4)to change delay value.

    Low

    7. Repeat for CO 2 high and low alarmdelays.

    CO 2 High Alarm Display

    High

    CO 2 Low Alarm Display

    Low

    11.04.4 Muting audible alarm

    To silence any audible alarm press .

    Muting will silence the audible alarms for 15minutes. The visual red "High" or "Low"indicator will continue to be displayed until thealarm condition is corrected. If the condition isnot corrected within 15 minutes, the audiblealarm will sound again.

    If an alarm is currently muted, the presence of an additional alarm condition will override theMUTE and the audible alarm will sound.

    If an alarm has been muted and the alarmcondition is removed and returns the audiblealarm will sound again regardless of whenMUTE was pressed.

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    11.04.5 Alarm Disable

    All alarms may be disabled for a period of up to 99 hours.

    To disable or change the alarm disable time:

    1. Press twice. Display shows

    2. Using or keys, enter the alarm

    disable time in hours.

    3. Press .

    The display will flash and a beep tone willconfirm that the value has been stored.

    4. To check alarm disable time remaining,

    press twice at any time.

    NOTE RECORD YOUR PASSWORD IN A SAFE LOCATION.

    While the keyboard is locked, setpoints,calibration and Hi-Limit safety values may beobserved but not changed.

    If an attempt is made to change previouslystored values while the keyboard is locked,the display will show

    11.05.2 Temporarily unlocking the keyboard

    The control panel can be temporarily unlockedto change a setpoint, calibration, or Hi-Limitsafety. The control panel will automatically re-lock after a new value has been entered.

    To temporarily unlock the keyboard:

    1. Press display will show

    2. Using or keys, enter your

    numerical password.

    3. Press

    The red LOCK light will now blink on andoff. Previously stored values may now bechanged. The keyboard will relock after the new value is entered.

    The keyboard will automatically re-lockafter any of the following actions:

    Ten seconds elapse with no buttonspressed.

    After is pressed whether a

    value was changed or not.

    11.05 Using the Keyboard Lock

    The keyboard may be locked to preventinadvertent changes to previously stored values.

    11.05.1 To lock the keyboard:

    1. Press display shows

    2. Using or keys, enter a numerical

    password of your choice.

    3. Press .

    Your password is stored, the control panelis now locked, and the red Lock indicator illuminates to indicate the locked condition.

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    1 1.05.3 Permanently unlocking the keyboard.

    The control panel can be permanentlyunlocked to allow operation withoutrestricting keyboard entry.

    When the control panel is unlocked, theincubator will accept changes topreviously stored values.

    To permanently unlock the incubator:

    1. Press display will show

    2. Using or keys, enter your

    numerical password.

    3. Press to temporarily unlock the

    keyboard. The red LOCK light will nowblink on and off.

    4. Press a second time, display will

    show

    5. Using or keys, re-enter your

    numerical password.

    6. Press .

    The red LOCK light will extinguish and theincubator will be permanently unlocked.

    IF YOU HAVE FORGOTTEN OR LOST YOUR PASSWORD, ENTER 257

    11.05.4 Factory Reset

    NOTEFOLLOWING A FACTORY RESET THE INCUBATOR MUST BE RECALIBRATED PRIOR TO USE. FOLLOW THE INITIAL OPERATING & CALIBRATION PROCEDURE, SECTION 10.0.

    The incubator can be re-set to initial factoryconditions at any time. To reset the incubator:

    1. Press

    simultaneously, display will show

    2. Press until display reads

    3. Press .

    The incubator will beep, display

    and return to initial factory settings.

    The following table lists all parameters thatare reset and their reset values.

    11.06 Door Heater Duty Cycle

    The control circuitry has the capability of controllingthe incubators door heater semi-separately for thepurpose of eliminating any condensation build upalong the outer perimeter of the inner glass door.There is a parameter "Uar" which is accessiblethrough a configuration display where the user canchange its value.

    Parameter Reset Value

    Temp. Setpoint 0

    Temp. CalibrationOffset

    0C

    Temp. Hi-Limit Safety 50C

    CO2 Setpoint 0

    CO2 Calibration Offset 0%

    Temp. Lo Alarm Delay 10 Minutes

    Temp. Hi Alarm Delay 5 Mi nutes

    CO2 Lo Alarm Delay 10 Minutes

    CO2 Hi Alarm Delay 5 Minutes

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    18

    The value of "Uar" has a range of 1.0 to 3.0. When"Uar" is 1.0, the door heater is controlled in thesame manner as the other heaters in the incubator.When "Uar" is increased above 1.0, the door heater remains on proportionally longer than the other heaters when the chambers temperature is withinthe proportioning band. For example, when "Uar"is set to 2.0, the door heater will be on twice as longas the other heaters.

    The factory setting for "Uar" is 1.0. This valueallows condensation to form on the glass door.Certain applications require that the glass door befree from condensation. Set "Uar" to an initial valueof 1.8. Allow the incubator to stabilize for 24 hrs.Check the condition on the glass door. Increasethe "Uar" value by 0.1 if the condensation is morethan required. Decrease the "Uar" value by 0.1 if the condensation is less than required. Thedetermination of the value for "Uar" is one of trialand error. Allow 24 hours for the incubator tostabilize before adjusting "Uar" to a new value.

    To change the value of "Uar":

    1. Press and hold and a n d

    then press . Display will show a

    sequential test pattern.

    2. Press and hold and and then

    press again. The displays will

    show:

    3. Press until "7" is

    displayed in thetemperature window and then press

    .

    The displays will show:

    12.0 CARE AND CLEANING OF STAINLESS STEEL

    NOTETHE FOLLOWING IS A GENERAL WRITE UP INTENDED AS INFORMATION. IT CAN BE USEDON INCUBATORS OR WATER BATHS.

    BEFORE USING ANY CLEANING OR DECONTAMINATION METHOD EXCEPT THOSE LISTED IN THE FOLLOWING, USERS SHOULD CHECK WITH PRECISION SCIENTIFIC THAT THE PROPOSEDMETHOD WILL NOT DAMAGE THE EQUIPMENT.

    Stainless steel is an alloy of steel with chromiumand nickel which increase the metal's resistanceto rust and corrosion. However, if not properlycared for it can rust and corrode. Exposure to air provides the passivations for clean stainless steel.This exposure produces a thin, durable chromium-oxide film that forms rapidly on the alloy surfaceand gives stainless steel its characteristic"stainless" quality. Exposure of the surface towater and other oxidizing environments alsoproduces this passivating film. However, if freeoxygen is not available due to scale or contaminationbuildup the metal surface may become vulnerableto attack.

    Maintaining a neutral pH and frequent cleaning withdetergent and water will give you years of troublefree service for your incubator or water bath. The

    following are some guidelines to consider.

    Water Distilled water is recommended. Maintain pHbetween 7 and 9 to minimize corrosion of thestainless steel.

    De-ionized or reverse-osmosis water may be used.However, if this water is very pure it may be

    4. Use or kets to select the

    desired value of "Uar" and then press

    . The displays will flash and then

    return to their normal operating states.

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    20

    1. Bathroom tub and tile cleaners. Availableat supermarkets.

    2. Any of a variety of "scale removers"available at your local supermarket or hardware store. Generally sold for cleaningcoffee makers, humidifiers andvaporizers.

    3. Citric acid based cleaners. Contact your lab supply dealer.

    4. A 15 to 35% phosphoric acid solution. Available form chemical supply dealersfor scale and rust removal. Allow solutionto soak the surface affected until rust andscale is loosened. Immediately rinse withcopious amounts of clean water.

    5. Oxalic Acid 2% to 5% in warm water.Swab solution on surface allowing it toremain until rust is loosened. Immediatelyflush with copious amounts of clean water.

    6. A mixture of 20% nitric acid and 1.5%hydrofluoric acid (or hydrochloric acid).Swab solution on surface allowing it toremain until rust is loosened. Immediatelyflush with copious amounts of clean water.

    The above are ranked in order of mild to strongcleaning agents. Items 5 and 6 should only be usedif severe rust and scale stains have developed andcannot be removed by any other method. In anycase the chemical should be allowed to do the

    cleaning with minimal scrubbing. Always followthe manufacturer's instructions.

    Chamber Disinfecting

    Materials known to be effective in disinfecting are:

    *Household Bleach*Glutaraldehyde*Alcohol

    Consult with your staff chemist for advice on usingthese chemicals.

    CAUTION ALWAYS RINSE WITH COPIOUS AMOUNTS OF CLEAN WATER. AIR DRY AND/OR FILL WITH FRESH WATER AND FOLLOW THE GUIDELINES IN "WATER"

    ABOVE.

    DISCLAIMERTHE ABOVE INFORMATION IS THE RESULT OF LIMITED INVESTIGATION AND NAPCO MAKES NOCLAIMS AS TO THE SUITABILITY TO YOUR PARTICULAR APPLICATION. THESE ARE INTENDEDTO BE GUIDELINES ONLY. CONSULT YOUR STAFF CHEMIST TO DETERMINE WHAT WORKS BEST IN YOUR LAB.

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    21

    Procedure1. Verify that setpoint temperature is greater than actual

    temperature.

    2. Check heaters. A. Disconnect power cord. Remove rear cover.B. Disconnect connectors J6 (bottom left) and J7

    (bottom) from power supply board.C. Check heater resistances with an ohmmeter.

    Approximate heater resistances (at ambienttemperature) are shown below:

    Heater Connections Resistance (in ohms)115V ~

    Air J6 Pins 1 & 3 132Water #1 J7 Pins 4 & 5 144Water #2 J7 Pins 5 & 6 144

    Au x J7 Pins 7 & 8 576J7 Pins 8 & 9 576

    Door J7 Pins 1 & 2 120

    D. Replace any heater that does not match theapproximate resistances above.

    3. Check TRIAC A. Turn power on.B. Place an AC voltmeter between pins 1 & 3 on wire

    side of connector J6 on power supply board.C. Set temperature setpoint below actual chamber

    temperature. Voltmeter should read 0 VAC.D. Set temperature setpoint to at least 5C above

    actual chamber temperature. Voltmeter shouldread line voltage.

    E. If voltmeter does not show correct values in either C or D, replace power supply board.

    4. Check Safety Relay A. Turn power on.B. Place an AC voltmeter between chassis ground

    and right hand side of R38 on power supply board.C. Set temperature setpoint to at least 5C above

    actual chamber temperature.D. Voltmeter should read line voltage. If voltmeter

    does not read line voltage, replace power supplyboard.

    13.0 Troubleshooting Procedures

    Problem13.01 No Heat

    WARNINGREFER SERVICING TO QUALIFIED SERVICE PERSONNEL. WHEN POWER IS APPLIED DANGEROUSVOLTAGES EXIST WITHIN THE CHASSIS COMPONENTS. USE EXTREME CARE WHEN MEASURINGVOLTAGES ON A LIVE CIRCUIT.

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    Problem ProcedureD. There should be an audible "click" from the solenoid

    each time the valve is actuated. If not, replacesolenoid.

    6. Check CO 2 Sensor

    The CO 2 sensor used in this unit is a thermal conductivitythermistor type. Two thermistors are connected in seriesand have a common connection.The approximate in-circuit voltage across each junctionat 37C is 2.1 VDC and at 25C is about 2.5 VDC. Theresistance of the sensor thermistors will vary withtemperature but a general range of resistance, asmeasured between Pins 1 & 2 or between Pins 2 & 3should be between 2K ohms to 4K ohms. Each junctionwill have about 1.5K ohms of resistance at 37C.

    1. Actual CO2 percent does not agree with displayed CO

    2percent, displayed CO 2 percent drifts or will not injectCO 2, unit will not hold a calibration. May signal possibleproblems with the IRCO 2 sensor.

    2. After following the CO 2 calibration procedure Section10.08, if a problem continues the sensor or Power PCBmay be defective. Test the 5 VDC supply at the Power PCB (between TP2 and TP3). After eliminating the power supply as a potential problem check the signal output atthe IRCO 2 sensor. Connect a digital voltmeter NEG leadto jack J1 lead labeled COM (ground). There are 2

    COM leads, either lead can be used. Connect the digitalvoltmeter POS lead to jack J1 lead labeled LINEAR(signal output). This linear function gives 0.0 VDC to 1.0VDC output which equates to 0.0% CO 2 to 20.0% CO 2.

    3. With the temperature and humidity stable for at least two(2) hours and with a CO 2 setpoint of 0.0% CO 2 and noCO 2 in the chamber, adjust the FINE ZERO potentiometer until the digital voltmeter reads 0.0 VDC+ 0.01 VDC. Enter a CO 2 setpoint between 5.0% CO 2 to 10.0% CO 2 andallow it to stabilize for about ten (10) minutes. Measure theCO 2 with a Fyrite. Multiply the measured CO 2 percentage

    by a factor of 0.05 and the value obtained will be theIRPCB output voltage, e.g.5.5% - CO 2 measuredX 0.050.275 VDC IRPCB volts out

    Slowly adjust the SPAN potentiometer until the proper outputvoltage is obtained. After completing the above procedure,follow the CO 2 calibration procedure Section 10.06.

    13.02 Poor CO 2 Control (TC Sensor) continued

    13.03 Poor CO 2 Control (IR - CO 2 Sensor)

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    24

    ProcedureProblem1. Check magnetic door gasket.

    A. Does the gasket fit evenly along the incubator body?There should be no distortions in the gasket that couldcause air to flow between the gasket and the incubator.

    B. If distortions exist, remove door liner and reposition or

    replace gasket.2. Check glass door gasket.

    A. Check for gasket tears or imperfections. Replacegasket, if imperfections found.

    B. Verify that the gasket is completely adhered to theincubator chamber. If not, apply RTV Silicone sealant(Dow Corning #732) to gasket and chamber. Allow tocure for 24 hours.

    C. Shut and latch the glass door firmly in place. Thegasket/ glass door interface should be completelysealed around the entire perimeter of the glass door.Try to place a business card between the glass door and gasket in any suspect areas. If a business cardcan be placed between the gasket and glass door,replace the gasket or check glass door alignment.

    3. Check glass door alignment. A. Check that the two plastic nuts are secured tightly to

    the two stainless steel hinges.B. If the door does not appear to be properly aligned,

    loosen the four capnuts holding the hinges onto theincubator and re-align the glass door.

    C. Repeat step 2C to verify the glass door alignment.

    Humidity within the chamber can vary with changing temperature,ambient conditions, and the nature of the sample. Thecondensation, or lack thereof, on the glass door is by no meansa proper way to measure the relative humidity within thechamber, but as a general rule there will be a very slight amountof condensation near the edges of the glass door during normaloperation or sometimes no condensation whatsoever. If all of the glass or greater than half the surface area of the glass iscovered with moisture, this may indicate a door heater problem.

    4. Check door heater. A. Turn power off.B. Disconnect connector J7 from the power supply board.C. Check the heater resistance by placing an ohmmeter

    between Pins 1 and 2. See section 13.01 for resistancevalues.

    If all results by following 13.04 are o.k., see section11.06 for door heater control.

    13.04 ExcessiveCondensation

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    25

    Problem ProcedureNoise inside the incubator chamber, above what would normallybe expected, is usually an indication of fan blade or fan motor trouble. Problems with excessive humidity and poor temperatureuniformity can also signal fan motor or fan blade trouble. The fanblade mounts to the fan motor shaft by friction. The proper rotationof the fan blade, observing the blade from within the chamber, isclockwise. Air is drawn into the fan and is blown out tangent to thefan blade. Buzzing noises can be isolated by checking the fanblade position on the shaft; it may be too close to the plenum, or pushed too far back against the fan motor plate.

    13.05 Noisy Fan

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    26

    14.0 Part Replacement Procedures

    WARNINGDISCONNECT POWER CORD BEFORE PERFORMING

    ANY OF THE FOLLOWING PROCEDURES.

    14.01 Temperature Sensor

    1. Disconnect incubator from power source.2. Remove the six (6) screws which secure rear

    cover plate.3. Locate jack J5 on the main PCB and disconnect.4. Pull out sensor from fan motor plate.5. Reinstall new sensor, reversing above procedure.

    NOTEWHEN INSTALLING NEW PROBE MAKE CERTAIN THE FLARE AT THE CABLE END OF THE PROBE STOPS AT THE BLACK GROMMET ON THE FAN MOTOR PLATE.

    6. Recalibrate temperature control, if necessary.Follow procedure in Section 10.05.

    14.02 TC CO 2 Sensor

    1. Disconnect incubator from power source.2. Remove the six (6) screws which secure rear

    cover plate.3. Locate jack J3 on the main PCB and

    disconnect.4. Remove the four (4) screws which fasten the

    CO 2 sensor to the fan motor plate.5. Reinstall new sensor, by reversing the above

    procedure.6. Allow the temperature and humidity to stabilize

    for at least two (2) hours. Recalibrate the CO 2control following the procedure in Section 10.08.

    14.03 Fan Motor

    1. Disconnect incubator from power source.2. Remove the six (6) screws which secure rear

    cover plate .3. Locate jack J6 on the main PCB and remove

    the leads, remove the ground lead from themotor as well.

    4. Remove the shelves and plenum from thechamber. Pull fan blade off shaft, removeretaining ring and two (2) screws which securemotor to fan motor plate.

    5. Reinstall the new motor, carefully noting therotation of the shaft. Reverse the aboveprocedure.

    NOTETHE MOTOR IS MULTIPLE VOLTAGE. THERE ARE FOUR (4) LEADS FROM THE FIELD WINDINGS.

    ATTACH LEADS AS FOLLOWS:

    WHITE AND RED TOGETHER TO NEUTRAL. BROWN AND RED TOGETHER. BLACK IS HOT .

    14.04 Air Heater

    1. Disconnect incubator from power source.2. Remove the shelves, supports, and plenum

    from within the incubator chamber.3. Remove the six (6) screws which secure rear

    cover plate. Note: It is not necessary to removethe fan motor plate to change the air heater .

    4. Disconnect the spade terminals leading fromthe main PCB at heaters ends.

    5. Remove the two (2) nuts which secure theheater to the fan motor plate and pull heater out through the front of the fan motor plate.

    6. Reinstall new heater, reversing the aboveprocedure.

    14.05 Water Jacket Heaters

    1. Disconnect incubator from power source.2. Remove the six (6) screws which secure rear

    cover plate.3. Locate the water jacket heaters, one left, one

    right at rear, near the bottom of the jacket.Remove the nut and retaining washer whichsecure the heater in the thermowell.

    4. Locate jack J7 on the main PCB anddisconnect the heater leads.

    5. Remove the heaters from the thermowells.Note: Grasp the heater sheath with pliers if heater will not easily slide out. Do not pullthem out by the leads, as they may rip out andleave the heater sheath stuck on the

    thermowell tube.6. Reinstall new heaters, reversing the aboveprocedure.

    14.06 Door Heaters

    1. Disconnect incubator from power source.2. Remove the screws which secure the front

    top panel.

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    14.11 IR CO 2 Sensor

    1. Disconnect power from incubator.2. Remove the screws which secure the

    rear cover plate.3. Locate jack J2 on PCB and disconnect.4. Remove two screws from outside of

    shield then remove the four screwswhich secure the sensor and rear shield.

    5. Install new sensor, carefully replacethe metal shield near the sensor,reversing the above procedure.

    6. Allow temperature and humidity torecover for at least four (4) hours andfollow the CO 2 Calibration Procedure inSection 10.08.

    14.09 CO 2 Solenoid Valve

    1. Disconnect incubator from power source.2. Remove the four (4) screws which secure

    the rear cover plate.3. Locate jack J13 and unplug connector.4. Disconnect hoses from defective valve

    assembly.5. Remove valve assembly.6. Install new valve assembly, reversing the

    above procedure.

    14.10 Display/Keyboard PCB

    1. Disconnect incubator from power source.2. Remove the screws which secure the inner

    liner of outer door. These screws are under the outer door gasket. Rest inner liner toprevent damage to heater.

    3. Peel back insulation to expose control board.Disconnect cable to connector of board.Remove four (4) nuts securing the board tothe door and remove board. Install new board,reversing the above procedure.

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    F1

    DOUBLEUNIT,LOWER

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    (ABOVECHAMBER)

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    ONLY ONE SENSORTYPE

    CAUTION:

    IRCO2SENSOR

    BLU

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    RED

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    WATER

    8

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    J51

    1 1 5 V

    1 1 5 V

    2 3 0 V

    2 3 0 V

    J7

    1 1

    2

    2

    3

    45

    JP1

    3

    4

    5

    6

    7

    T

    HERMOSTAT

    LIMITING

    BLK

    60watt

    R E D

    B L K

    230V3

    9

    2 1

    J16

    110V

    1JP2

    4

    115V 2

    3

    J14

    1

    BLU

    J4

    11J3J2J1

    5BLU

    1BLU

    DOOR MOUNTED

    BOARD

    J13

    1ORG

    ORG

    NOTE: Heaters resistanceis with

    G R A Y

    Toleranceis+5%, -10%

    andconnectorremovedfromPCboard.

    element at ambienttemperature,

    100W PERELEMENT

    HEATER,CARTR.

    100V

    100

    100

    WHT

    BLK

    WIRE NUT

    WHT

    BLK

    200

    YEL

    WHT

    BLK

    83

    (ABOVECHAMBER)

    100V12

    0W,100V

    HEATER,DOOR

    HEATER,AUX.50W

    F1- 100/115Volt6.3Amp

    J9

    3

    POWER

    T160mAL250V

    TRANSFORMER

    0.3AMP SB

    IN

    WHT

    21 F1

    BLK

    G R N / Y E L

    CHASSIS

    GROUND

    BOARD

    PCB POWER

    T P 2

    T P 3

    T P 4REC.

    TEMP

    OUTPUT

    ALARM

    J9

    7.0 VOLT

    R5

    GRN/YEL

    N/O

    N/C

    COM

    +-

    { {J11 J12

    T C C O 2

    G N D

    T P 1

    + 5 A

    U N R E G

    BLK

    WHT

    13C

    4

    5SWITCH

    MAIN

    DPST

    WHT

    BLK

    2

    1

    5 6

    WHT

    1

    12

    CONTROL

    1 2 3

    6 5 4 3 21

    TEMP. SENSOR (OPTIONAL)

    EXTERNALBACKUP

    (OPTIONAL)

    TEMPERATURE CONTROL

    EXTERNALBACKUP

    4

    1

    2

    3

    B R O W N

    J7 5 6

    7

    8

    9

    RED

    BLK

    10mV/C

    34234222-100/115V

    34234226-230V

    BLK

    WHT

    BLK

    DS1

    LIGHT:

    GRN/YEL

    3 1 2

    R E D W H T

    52

    4

    TO CHASSISG

    1

    YEL

    GRN/YEL

    BLU

    GROUND

    BLK

    ORG

    GROUNDG1

    G R N / Y E L

    B L K

    TO CHASSIS

    WHT

    BLK

    J-16

    6

    BLK

    WHT

    SWITCH

    MAIN

    DPST

    F1

    6C9

    8 12

    BLK

    8

    H FILTER

    EMI/RFI

    11

    GRN/YEL

    H

    N

    N G

    TO CHASSIS

    GROUND

    G1

    13

    WHT

    H

    74

    GN

    CORD

    POWER

    GRN/YEL

    B L K

    G R N / Y E L

    BLK

    11

    W H T(LINE)

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    WarrantyNAPCO warrants its products against defects in material or in workmanship when used

    under appropriate conditions and in accordance with appropriate operating instructions for a periodof no less than one (1) year from the date of delivery of the products.

    The sole obligation of NAPCO shall be to repair or replace at our option, FOB factory or locally, without charge, any part(s) that prove defective within the warranty period, provided thatthe customer notifies NAPCO promptly and in writing of any such defect. Compensation for labor by other than NAPCO employees will not be our obligation. Part(s) replacement does not constitutean extension of the original warranty period.

    NAPCO makes no warranty of merchantability, fitness for a particular purpose, or any other warranty, express or implied, as to the design, sale, installation, or use of its products, and shallnot be liable for consequential damages resulting from the use of its products.

    NAPCO will not assume responsibility for unauthorized repairs or failure as a result of

    unauthorized repairs, replacement, or modifications made negligently or otherwise improperlymade or performed by persons other than NAPCO employees or authorized representatives.

    While our personnel are available to advise customers concerning general application of allmanufactured products, oral representations are not warranties with respect to particular applicationand should not be relied upon if inconsistent with product specification or the terms stated herein.

    In any event, the terms and conditions continued in NAPCO , formal sales contracts shall becontrolling; and any changes must be in writing and signed by an authorized executive of NAPCO.

    All defective components will be replaced without charge for one (1) year from the date of delivery. There will be no charge for labor if the apparatus is returned to the factory prepaid.

    Conditions and qualifications of the warranty statement shall prevail at all times.