management of metabarcoding data -...
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Management of metabarcoding data
Urmas Kõljalg
University of Tartu, Estonia
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UNITE Milestones
2001 – rDNA ITS was adopted as a marker gene for the
fungal reference database
2003 – first online rDNA ITS based key for few hundred
species
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UNITE Milestones
2007 - curated rDNA ITS datasets from any biological
sample (specimen, living specimen, soil, water, air,
medical, etc.)
2007 – online curation and third-party annotations of INSD
(GenBank, ENA, DDBJ) sequences
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UNITE Milestones
2011 – online curation of Species Hypotheses
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Communication of Species Hypotheses in
Research Papers
Accession codes like :
SH184362.07FU
are resolved with unique DOI (digital object identifier):
dx.doi.org/10.15156/BIO/SH184362.07FU
Version 7 includes 487 435 SHs and each of them have
unique DOI
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Communication of Species Hypotheses
in Research Papers
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Identification and Communication of Fungal
Species
All SHs are freely downloaded at unite.ut.ee
For the inhouse analyses or for the building identification
pipelines
HTS pipelines: QIIME, mothur, SCATA, CREST,
USEARCH, UCHIME, UTAX.
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High-Throughput Seguencing
projects
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www.plutof.com
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Management of metabarcoding data
Starting new project
Uploading data from published study
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Thank you!
Kessy Abarenkov Allan Zirk