maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and...
TRANSCRIPT
![Page 1: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/1.jpg)
Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease
Mary Ann Stepp, Ph. D.Professor
Department of Anatomy and Cell Biology and of Ophthalmology
![Page 2: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/2.jpg)
How are the corneal epithelial stem cells maintainedthroughout our lifetime within their niche at the limbusdespite all the insults the ocular surface is forced to endure?
![Page 3: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/3.jpg)
Some problems that can arise on the ocular surface…
After PRK RecurrentErosion
Corneal EpithelialStem Cell Deficiency
(CESCD)
![Page 4: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/4.jpg)
Some problems that can arise on the ocular surface…
After PRK RecurrentErosion
Corneal EpithelialStem Cell Deficiency
(CESCD)
Development of mouse model for the study of these conditions
![Page 5: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/5.jpg)
The cornea is optically wellsuited for microscopic analysis
QuickTime™ and aCinepak decompressor
are needed to see this picture.
4 integrin whole mount
![Page 6: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/6.jpg)
The cornea is optically wellsuited for microscopic analysis
QuickTime™ and aCinepak decompressor
are needed to see this picture.
4 integrin whole mount
QuickTime™ and aCinepak decompressor
are needed to see this picture.
![Page 7: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/7.jpg)
![Page 8: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/8.jpg)
![Page 9: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/9.jpg)
To get at the mechanism of these affects, we have a cell culture model we are using to study cell adhesion and migration.
QuickTime™ and aCinepak decompressor
are needed to see this picture.
![Page 10: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/10.jpg)
To get at the mechanism of these affects, we have a cell culture model we are using to study cell adhesion and migration.
![Page 11: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/11.jpg)
To get at the mechanism of these affects, we have a cell culture model we are using to study cell adhesion and migration.
![Page 12: Maintaining the ocular surface of the eye: the role of corneal epithelial stem cells in health and disease Mary Ann Stepp, Ph. D. Professor Department](https://reader035.vdocuments.us/reader035/viewer/2022062517/56649eec5503460f94bfe4ff/html5/thumbnails/12.jpg)
Many important questions remain but we believe we have a model system that continues to give us new insight into the how
cell adhesion regulates the stem cell niche.
THANK YOU FOR YOUR ATTENTION!