CHEMICAL, MECHANICAL AND CHEMICAL, MECHANICAL AND ANTIMICROBIAL PROPERTIES OF ANTIMICROBIAL PROPERTIES OF

SPUTTERED SILVER SPUTTERED SILVER NANOCLUSTER-SILICA COMPOSITE NANOCLUSTER-SILICA COMPOSITE

THIN FILMSTHIN FILMSM. Ferraris, S. Perero, M. Miola, M. Ferraris, S. Perero, M. Miola, S. FerrarisS. Ferraris, A. , A. Battiato, C. Manfredotti, E. Vittone, E. VernèBattiato, C. Manfredotti, E. Vittone, E. Vernè

THE PROBLEMTHE PROBLEM

Bacterial Bacterial contaminationcontamination

Medical devicesMedical devices

Surfaces for everyday-lifeSurfaces for everyday-life

Surfaces used by hundreds of Surfaces used by hundreds of peoplepeople

… …

AIM of THE AIM of THE WORKWORK

Obtain an effective and versatile antibacterial Obtain an effective and versatile antibacterial layerlayer

Stable (thermally, chemically … )Stable (thermally, chemically … )

Applicable to different surfacesApplicable to different surfaces

With tailorable antibacterial activityWith tailorable antibacterial activity

Broad spectrum of activityBroad spectrum of activity

Low resistance developmentLow resistance development

This research activity is supported by:This research activity is supported by:

Regione Piemonte, Italy, with ‘‘NABLA- Nanostructured Regione Piemonte, Italy, with ‘‘NABLA- Nanostructured Antibacterial Layers.’’Antibacterial Layers.’’

EU Project-NASLA-FP7-SME-2010-1 (Project 262209)EU Project-NASLA-FP7-SME-2010-1 (Project 262209)

SILVERSILVER

well known antibacterial well known antibacterial agentagent

broad spectrum activitybroad spectrum activity

Low resistance Low resistance developmentdevelopment

SILICASILICA

Thermal, Thermal, chemical, chemical, mechanical mechanical stabilitystability

SILICASILICA

AgAg

SILICA TARGETSILICA TARGET SILVER TARGETSILVER TARGET

SPUTTERINGSPUTTERING

SILICASILICA AgAg

ArAr ArAr

SUBSTRATESUBSTRATE

SUBSTRATESUBSTRATE

SILVER NANOCLUSTERS-SILICA SILVER NANOCLUSTERS-SILICA ANTIBACTERIAL LAYERANTIBACTERIAL LAYER

CO-SPUTTERING: THE SILVER CO-SPUTTERING: THE SILVER NANOCLUSTERS-SILICA FILMNANOCLUSTERS-SILICA FILM

XPS CHARACTERIZATION OF THE XPS CHARACTERIZATION OF THE SPUTTERED LAYER (*)SPUTTERED LAYER (*)

528530532534536Binding Energy [eV]

0

10

20

30

40

50

60

70

Intensity[cps]

Filename: NablaAsDep

O1s

1|

2|

3|

O 1sO 1s

532.7 eV532.7 eV SiO2

Secondary Secondary componentscomponents carboxylic groups from contaminations

98100102104106Binding Energy [eV]

0

2

4

6

8

10

12

14

Intensity[cps]

Filename: NablaAsDep

Si2p1|

282284286288290Binding Energy [eV]

0

2

4

6

8

Intensity[cps]

Filename: NablaAsDep

C1s

1|

2|

3|

4|

Si 2pSi 2p

103.38 eV103.38 eV SiO2

(*) Experimental Physics Department /Centre of Excellence “Nanostructured Interfaces and surfaces”, University of Torino

282284286288290Binding Energy [eV]

0

2

4

6

8

Intensity[cps]

Filename: NablaAsDep

C1s

1|

2|

3|

4| C 1sC 1s

contaminantscontaminants

284.47 eV284.47 eV CH2

285.38 eV285.38 eV C-O and C-CO

288.49 eV288.49 eV COOH

364366368370372Binding Energy [eV]

0

10

20

30

40

50

Intensity[cps]

Filename: NablaAsDep

Ag3d 1|

Ag 3dAg 3d

368.48 eV368.48 eV Ag0

METALLIC SILVER METALLIC SILVER NANOCLUSTERS !NANOCLUSTERS !

M. Ferraris et al., ADVANCED BIOMATERIALS, 2010, ISSN: 1868-4505.

M. Ferraris et al., MATERIALS CHEMISTRY AND PHYSICS 120 (2010) 123–126.

As Dep. 150 ºC 300 ºC 450 ºC 600 ºCAs Dep. 150 ºC 300 ºC 450 ºC 600 ºC

MORPHOLOGICAL AND CHEMICAL MORPHOLOGICAL AND CHEMICAL CHARACTERIZATIONCHARACTERIZATION

M. Ferraris et al., ADVANCED BIOMATERIALS, 2010, ISSN: 1868-4505.

M. Ferraris et al., MATERIALS CHEMISTRY AND PHYSICS 120 (2010) 123–126.

GammGamma ster.a ster.

EtO EtO ster.ster.

As Dep. 150 ºC 300 ºC 450 ºC 600 ºCAs Dep. 150 ºC 300 ºC 450 ºC 600 ºC

ANTIBACTERIAL ACTIVITY: inibition halo ANTIBACTERIAL ACTIVITY: inibition halo S. aureusS. aureus

M. Ferraris et al., ADVANCED BIOMATERIALS, 2010, ISSN: 1868-4505.

M. Ferraris et al., MATERIALS CHEMISTRY AND PHYSICS 120 (2010) 123–126.

As Dep. 150 ºC 300 ºC 450 ºC 600 ºCAs Dep. 150 ºC 300 ºC 450 ºC 600 ºC

Tape Tape adhesioadhesionn

Scratch Scratch resistancresistancee

0,0

5,0

10,0

15,0

20,0

25,0

AS DEP 150 300 450 600

Fir

st

cra

ck l

oad

[N

]

20 m 20 m

20 m 20 m

20 m

MECHANICAL PROPERTIESMECHANICAL PROPERTIES

Ag+ RELEASE

0

10

20

30

40

50

60

70

80

0 5 10 15 20 25 30

Days

g

AD

150°C

300°C

450°C

600°C

M. Ferraris et al., ADVANCED BIOMATERIALS, 2010, ISSN: 1868-4505.

M. Ferraris et al., MATERIALS CHEMISTRY AND PHYSICS 120 (2010) 123–126.

SILVER RELEASE (*) SILVER RELEASE (*)

(*) Traumatology and Work Medicine Department of C.T.O (Turin)

COATINGS ON POLYMERS FOR BIOMEDICAL COATINGS ON POLYMERS FOR BIOMEDICAL APPLICATIONS APPLICATIONS

TAPE TEST (ASTM D TAPE TEST (ASTM D 3359):3359): good adhesion of the coating to the substrate.

Antibacterial properties are Antibacterial properties are maintained after maintained after sterilization (chemical and sterilization (chemical and radiation)radiation)

The deposited film The deposited film maintains its properties maintains its properties after the common washing after the common washing procedures included in the procedures included in the medical devices production medical devices production routesroutes

Stability and silver release Stability and silver release in physiological solution in physiological solution UNDER INVESTIGATIONUNDER INVESTIGATION

Cellular tests UNDER Cellular tests UNDER INVESTIGATIONINVESTIGATION

COATINGS ON POLYMERS FOR AEROSPACE COATINGS ON POLYMERS FOR AEROSPACE APPLICATIONS APPLICATIONS

TAPE TEST TAPE TEST (ASTM D (ASTM D 3359):3359): good adhesion of the coating to the substrate

3 21

112233

Antibacterial Antibacterial activity connected activity connected to silver contentto silver content

Antibacterial Antibacterial activity activity maintained after maintained after agingaging

COATINGS ON MATERIALS FOR MOBILE COATINGS ON MATERIALS FOR MOBILE TELEPHONES APPLICATIONS TELEPHONES APPLICATIONS

ACOUSTIC ACOUSTIC FELTFELT

SCREENSCREEN

0

20

40

60

80

100

120

140

160

180

1 2 3 4 5

campioni

CF

U/c

m2

0 1 2 3 4

COATINGS ON MATERIALS FOR FOOD INDUSTRY COATINGS ON MATERIALS FOR FOOD INDUSTRY APPLICATIONS APPLICATIONS

TAPE TEST TAPE TEST good good adhesionadhesion

MILK MILK

AGEINAGEIN

GG

ANTIBACTERIAL ANTIBACTERIAL EFFECTEFFECT

COATINGS ON MATERIALS FOR EQUIPMENTS AND COATINGS ON MATERIALS FOR EQUIPMENTS AND PERSONNEL PROTECTIVE SYSTEMS PERSONNEL PROTECTIVE SYSTEMS

CONCLUSIONS AND FUTURE DEVELOPMENTS CONCLUSIONS AND FUTURE DEVELOPMENTS

Silver nanoclusters-silica antibacterial layer has been Silver nanoclusters-silica antibacterial layer has been deposited on model silica substrates and characterized deposited on model silica substrates and characterized after different thermal treatmentsafter different thermal treatments

Co-sputtering deposition technique allow to vary Co-sputtering deposition technique allow to vary silver content of the layer in order to obtain different silver content of the layer in order to obtain different antibacterial activitiesantibacterial activities

The layer has been effectively deposited on different The layer has been effectively deposited on different substrates from industrial partnerssubstrates from industrial partners

Particular tests are under investigation by industrial Particular tests are under investigation by industrial partners in order to optimize the layer for specific partners in order to optimize the layer for specific applications applications

ThankThanks!s!