LS174T

p-EGFR

MEK 1/2

p-MEK 1/2

AKT

actina

RAS-T

RAS-Act

pAKT

EGFR

GEOHCT15

HCT116

SW48

0

SW62

0

p-MEK 1/2

actina

RAS-Act

p-EGFR

MEK 1/2

AKT

RAS-T

pAKT

GLC82

A549

Calu3

H460

H1299

EGFR

Fig. S1

AB

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

p.G12D c.35G>A LS174T

p.G12A c.35G>C GEO

p.G12V c.35G>T SW480;SW620

p.G13D c.38G>A HCT116; HCT15

p.G13A c.38G>C

p.G13V c.38G>T

p.G13G c.39C>G

p.G13G c.39C>A

p.G13G c.39C>T

p.G12S c.34G>A A549

p.G12R c.34G>C

p.G12C c.34G>T

p.A59T c.175G>A

p.Q61K c.181C>A

p.Q61E c.181C>T

p.Q61E c.181C>G

p.Q61P c.182A>C

KR

AS

Table S.1A Mutation Status of Cell Lines Used in This Study

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

p.Q61R c.182A>G

p.Q61L c.182A>T

p.Q61H c.183A>C H460

p.Q61H c.182A>T

KR

AS

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

p.Q61P c.182A>C

p.Q61L c.182A>T

p.Q61R c.182A>G

p.Q61H c.183A>C

p.Q61Q c.183A>G

p.Q61H c.183A>T

p.G61K c.181C>A H1299

p.G61E c.181C>G

p.G13S c.37G>A

p.G13R c.37G>C

p.G13C c.37G>T

p.G12R c.34G>C

p.G12S c.34G>A

NR

AS

Table S.1B Mutation Status of Cell Lines Used in This Study

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

p.K601E c.1801A>G

p.V600E c.1799T>A

p.D594G c.1781A>G

BR

AF

Table S.1C Mutation Status of Cell Lines Used in This Study

GENE AMINO ACID CHANGE

NUCLEOTIDE MUTATION

EXON CELL LINE

p.H1047R c.3140A>G exon 20 HCT116; GLC82; LS174T

p.H1047L c.3140A>T exon 20

p.P539R c.1616C>G exon 9

p.Q546K c.1636C>A exon 9 A549

p.Q546E c.1636C>G exon 9

p.E81K c.241G>A exon 1

p.R88Q c.263G>A exon 1

p.G106V c.317G>T exon 1

p.N345K c.1035T>A exon 4

p.R93W c.277C>T exon 1

p.S158L c.473C>T exon 2

p.H160N c.478C>A exon 2

p.R38H c.113G>A exon 1

p.E542K c.1624G>A exon 9

p.E542Q c.1624G>C exon 9

p.E545K c.1633G>A exon 9 H460;HCT15

p.E545Q c.1633G>C exon 9

PIK

3CA

Table S.1D Mutation Status of Cell Lines Used in This Study

GENE AMINO ACID CHANGE

NUCLEOTIDE MUTATION

EXON CELL LINE

p.G118D c.353G>A exon 2

p.G12D c.35G>A exon 1

p.H1047Y c.3139C>T exon 20

p.C420R c.1258T>C exon 7

p.H701P c.2102A>C exon 13

p.K184E c.550A>C exon 2

p.C901F c.2702G>T exon 18

p.M1004I c.3012G>T exon 20

p.G1007R c.3019G>C exon 20

p.G1049R c.3145G>C exon 20

p.R108H c.323G>A exon 1

PIK

3CA

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

p.R248W c.742C>T

p.Y220C c.659A>GP53

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

p.F241S c.722T>C

p.Y65C c.194A>G

p.E150Q c.448G>C

p.K62R c.185A>G

p.K267fs*9 c.800delA

PT

EN

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

p.R154W c.460C>T

p.E221 c.661G>T

p.S251N c.752G>A

p.I295fs*23 c.882_882delGME

K 1

/2

Table S.1E Mutation Status of Cell Lines Used in This Study

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

AKT p.A377V c.1130C>T

GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE

EGFR p.E746_A570 c.2236_2250del15

Table S.1F Mutation Status of Cell Lines Used in This Study

KRAS

PI3Kca

BRAF

NRAS

NO YES

RE 2 5

SE 3 1

Χ-test p =

RE SE

No mut 1 2

G12 2 3

G13 1 1

Χ-test p =

NO YES

RE 7 0

SE 3 1

Χ-test p = 1

NO YES

RE 1 4

SE 2 4

Χ-test p =

KRAS mutation NRAS mutationPIK3Ca mutationKRAS (G12and G13) mutationFig. S2

8

1 6

0

0 50

00

0.8466 0.72730.1939

Fig. S7

H460A549LS174THCT15GLC82CALU3HCT116H1299SW620SW480GEO H460A549LS174THCT15GLC82CALU3HCT116H1299SW620SW480GEO

Fig. S3

αs

β γ

GPCRRTK

Ras

Raf-1

MEK1/2

MAPK

ATP cAMP

PKAR

PKAC

AKAP13

RA signaling

Cell proliferationCell differentiation

Cell survivalCell proliferatiom

TAF3

RXRANR1H2

NCOA3

Fig. S4

P

PP P

Grb2

SOSShc

ADCY7