LPS, Microbial toxins

Innate Immunity · Acute phase proteins · Western blot · TLR · Assays · Antimicrobial peptides · Flow

cytometry · LPS, Microbial toxins · Robust · Complement Research · Easy to use · Scavenger receptors ·

Inflammation · Sensitivity · Unique products · Immunohistochemistry · Coagulation molecules · Quality control ·

Cytokines · Chemokines · Antibodies · Cell & Tissue damage · Functional studies · Lipid binding proteins ·

Immunoprecipitation · Oxidative stress · Specificity · Organ & Tissue related research · Proteins · Value for money

General introduction

Hycult Biotech develops, produces and markets antibodies, antibody based products and more specifically immunoassays for innate immunity and directly related fields, with an emphasis on complement, neutrophil proteins, TLR, scavenger receptors and acute phase proteins. Furthermore, we have a strong interest in progressing research in the area of inflammation and cell damage caused by pathogens or oxidation factors.

Introduction product groupEndotoxin and other microbial toxins are involved in infectious diseases as well as in many other pathologies like atherosclerosis. The unraveling of the Toll-like receptor’s function in immunity has raised new interest in the innate immune response to bacterial toxins. Moreover, involvement of the acute phase response with LPS-binding proteins like LBP and Serum Amyloid A (SAA), has also contributed to this attention.

More information about products within the field of Innate Immunity, Antimicrobial peptides, Microbial toxins, TLR, Inflammation, Cell & Tissue damage, Lipid binding proteins and Oxidative stress can be found on our website. Please feel free to subscribe to our newsletter to remain up-to-date on Hycult Biotech’s product information!

Hycult Biotech has worldwide distribution through a network of over 45 distributors.

If you have any questions regarding pricing and ordering, please contact your local distributor. Distributor information can be found on our website.

We believe technical support is essential when using our products or when looking into buying them for your research needs. For product specific information please visit our website: www.hycultbiotech.com or contact us on support@hycultbiotech.com.

In this Product Folder the following abbreviations for the applications have been used:FC: Flow CytometryFS: Functional studiesIA: ImmunoassaysIF: ImmunofluorescenceIHC: Immunohistochemistry F: Frozen sections P: Paraffin sectionsIP: ImmunoprecipitationW: Western Blot

macrophage

liver

tissue injury

TCC

PTX3

humoral innate immunity

bacteria

C1q

classicalcomplement

pathway

Factor H, Factor D, Factor Bbproperdin

alternativecomplement

pathway

IL-6

IL-1

SAA CRP haptoglobinfibrinogenceruloplasmin

HDL-3

C3, C4

SAP albumintransferrin

acute phaseresponse

complementactivation

increasedsynthesis

decreasedsynthesis

C3aC5a

Assays

Product description Quantity Cat.#

BPI, Human, ELISA kit 1 x 96 det. HK314-01

EndoCab IgA, Human, ELISA 1 x 96 det. HK504-IgA

EndoCab IgG, Human, ELISA 1 x 96 det. HK504-IgG

EndoCab IgM, Human, ELISA 1 x 96 det. HK504-IgM

EndoCab, Human, ELISA kit 1 x 96 det. HK504

LAL Chromogenic Endpoint Assay 3 x 96 det. HIT302

LBP, Human, ELISA kit 1 x 96 det. HK315-01

LBP, Mouse, ELISA kit 1 x 96 det. HK205-01

LBP, various species, ELISA kit 1 x 96 det. HK503

LL-37, Human, ELISA kit 1 x 96 det. HK321-01

Pentraxin 3, Human, ELISA kit 1 x 96 det. HK347-01

SAA, Human, ELISA kit 1 x 96 det. HK333-01

SAP, Mouse, ELISA 1 x 96 det. HK215-01

sCD14, Human, ELISA kit 1 x 96 det. HK320-01

SLPI, Human, ELISA kit 1 x 96 det. HK316-01

sTREM-1, Human, ELISA kit 1 x 96 det. HK348-01

TRACP5a, Human, ELISA kit 1 x 96 det. HK363-01

Product description Clone name Quantity Applications Cat. #

CD14, Human 18D11 100 µg FC FS HM2224CD14, Mouse Sa14-2 100 µg FC FS IF W HM1060CD14, Mouse, FITC Sa14-2 100 µg FC FS IF HM1060FCD14, Mouse, functional study antibody Sa14-2 0.5 mg FC FS IF HM1060-FSCD14, Mouse, R-PE labeled Sa14-2 25 tests FC FS IF HM1060PE-25CD14, Mouse, R-PE labeled Sa14-2 100 tests FC FS IF HM1060PE-100CNF1 NG8 100 µg FS IA W HM6005CNF1/CNF2 JC4 100 µg FS IA W HM6006EF-Tu, E.coli 900 100 µg IA IF W HM6010Lipid A 26-5 100 µg IA HM6009Lipid A, free 43 > 200 µg IA W HM2046Lipopolysaccharide Core WN1 222-5 100 µg FS IA P W HM6011Lipopolysaccharide Core, functional study antibody WN1 222-5 0.5 mg FS IA P W HM6011-FSLipopolysaccharide Core, immuno assay antibody WN1 222-5 0.5 mg FS IA P W HM6011-IALipoteichoic acid 55 > 200 µg F IA W HM2048LRG, Human 2F5.A2 100 µg IA HM2013Pentraxin 3, Mouse 2C3 100 µg FC FS IA IF IP W HM1130Pentraxin 3, Mouse 6B11 100 µg FC F FS IA W HM1131Polymyxin B 45 > 200 µg IA W HM2047Shigatoxin 1 13C4 100 µg FS IA W HM6007Shigatoxin 2 11E10 100 µg FS IA W HM6008sTREM-1, Human 15G7 100 µg IA HM2304TREM-1, Human 6B1 100 µg FC FS IA W HM2252TREM-1, Mouse L5-B8 100 µg FC FS IF W HM1098TREM-1, Mouse, FITC L5-B8 100 µg FC FS IF W HM1098FTREM-2, Mouse 6E9 100 µg FC IA IP HM1129TREM-2, Mouse, FITC 6E9 100 µg FC IA IP HM1129F

Antibodies

Figure 2: Serum Amyloid A deposits in glomerulus and arteriole of a kidney from a patient with amyloidosis. Staining with antibody Reu 86.1 (Cat.# HM2100).

Figure 3: Mouse CD14 in RAW cells. Staining with antibody Sa14-2 (Cat.# HM1060).

Figure 1: LPS staining within the colon lumen of SIV-infected rhesus macaques using antibody WN1 222-5 (Cat.# HM6011). Image courtesy of Dr. Jacob D. Estes from the Frederick National Laboratory for Cancer Research in Maryland, USA.

Hycult Biotech Frontstraat 2A5405 PB UdenThe Netherlands

T +31(0)413 251335F +31(0)413 248353E info@hycultbiotech.com

Hycult Biotech is distributed by:

Why scientists buy our products:· Robustness · Sensitivity · Specificity · Easy to use · Value for money · Two-plate kits · Functional studies · Technical support · · Validated assays · Quality control · Innate focus · Well documented products · Broad in applications ·

Hycult Biotech Inc.600 West Germantown PikeSuite 110Plymouth Meeting, PA 19462USAT: +1 484 5343566 Toll Free: 855-2-HYCULT (855-2-492858)F: +1 484 3683558E: orders-us@hycultbiotech.com

Ligands

Proteins / Peptides

Product description Quantity Cat.#

Product description Quantity Cat.#

Lipid A from E.coli, Serotype R515 (Re) 1 mg HC4049

Lipid A from Salmonella minnesota, R595 (Re) 1 mg HC4057

LPS from E.coli Serotype O55:B5 1 mg HC4045

LPS from E.coli, Serotype EH100 (Ra) 1 mg HC4046

LPS from E.coli, Serotype J5 (Rc) 1 mg HC4047

LPS from E.coli, Serotype O8:K27 1 mg HC4065

LPS from E.coli, Serotype R515 (Re) 1 mg HC4048

LPS from Salmonella abortus equi, S-form 1 mg HC4061

LPS from Salmonella enteriditis, S-form 1 mg HC4059

LPS from Salmonella minnesota, R345 (Rb) 1 mg HC4053

LPS from Salmonella minnesota, R5 (Rc) 1 mg HC4054

LPS from Salmonella minnesota, R595 (Re) 1 mg HC4056

LPS from Salmonella minnesota, R7 (Rd) 1 mg HC4055

LPS from Salmonella minnesota, S-form 1 mg HC4051

LPS from Salmonella Minnesota. R60 (Ra) 1 mg HC4052

LPS from Salmonella typhimurium, S-form 1 mg HC4060

LPS set: 2 x LPS, 1 x Lipid A and 1 x MPL-A from E.coli 2x 0.5 mg and 2x 0.25 mg HC4064

LPS set: 2 x LPS, 1 x Lipid A and 1 x MPL-A from Salmonella minnesota 2x 0.5 mg and 2x 0.25 mg HC4063

MPL-A from E.coli, R515 (Re) 0.5 mg HC4050

MPL-A from Salmonella minnesota, R595 (Re) 0.5 mg HC4058

LALF, Peptide 1 mg HC4031-10

LALF, Peptide 0.5 mg HC4031-05

LALF, Peptide 100 µg HC4031-01

LBP, Human, Peptide 100 µg HC4030-01

LBP, Human, Peptide 0.5 mg HC4030-05

LBP, Human, Peptide 1 mg HC4030-10

LL-37/CAP-18, Human, Peptide 50 µg HC4013

Polymyxin B (PMB) 10E5 units HC4020

Polymyxin B (PMB), biotinylated 0.2 ml HC4021

Polymyxin B (PMB), HRP-conjugated 0.2 ml HC4022

Figure 4: Western blot detection of differentconcentrations of purified Proteinase 3 with mAbPR3G-2. The band size is approximately 30kDa(Cat.#HM2172).

Figure 5: Flow cytometric detection of granulocytesand monocytes with monoclonal antibody to MouseTREM-1 (mAb L5-B8, Cat.#HM1098).