liquid handling & pcr product guide - alfagen · 3103-00s 12-strip dome caps dome pcr 80 strips...

12
Liquid Handling & PCR Product Guide

Upload: trannhi

Post on 09-Nov-2018

214 views

Category:

Documents


0 download

TRANSCRIPT

Liquid Handling & PCR Product Guide

Liquid Handling & PCR Product Guide

ALFAGEN MEDICAL SUPPLIES • Call us: +9-0232-375-25-30; +9-0232-344-25-30 • Mail us: [email protected]

LOW RETENTIONVertex tips feature our specially formulated NoStick® resin which provides an even higher degree of fluid confidence and control. The inner surface of our Vertex tip is considerably more hydrophobic than standard industry tips enabling viscous fluids to be dispensed more completely. There is less fluid retention and less sample loss. Vertex tips prove that less is more when it comes to liquid handling!

CLEAN LIQUID RELEASEOur tips are purposely designed for a much cleaner liquid release. Our thin walls and finely tapered tip construction eliminates the need to touch off. Users will maintain complete fluid control working with smaller volumes, and not compromise their pipetting accuracy or precision.

THE ECOPAC® REFILL SYSTEMThe EcoPac® Refill System provides handy lift-and-load simplicity. Our new system does not require any tools or special instructions to move from an empty insert to a fresh set of 96 tips.

EcoPac® Refill Systems are environmentally friendly, they reduce lab waste by 70% and require less shelf space than standard racks.

• Manufactured from High Density Polyethylene (HDPE).

• Filter tips act as a barrier to protect nose cone of pipettor and sample from contamination, but does not impede air flow.

• Filter tips material is hydrophobic and does not contain additives that can contaminate; nor will it absorb samples.

• Certified RNase, DNase, DNA and PCR inhibitor free.

• Certified non-pyrogenic.

• Sterilized.

Vertex Pipette Tips

COM

PETI

TOR'

S T

IP

VERT

EX T

IP

• Our tip rack was designed to be a time saver as it allows for one hand operation. A simple press on the front of the lid releases it from the rack; otherwise the lid sits securely closed.

• Our EcoPac® products save precious time in loading, and also save on storage space and plastic.

• Tip inserts are imprinted with tip size and lot number data that are visible even when seated inside the rack.

• EcoPac® refill trays are robust and designed to withstand multiple autoclave cycles.

• Our tip rack was designed to facilitate offset pipetting. The edge of the rack and insert are out of the way so users can easily load four tips onto a multichannel pipette.

• EcoPac® refill cases are made from paper that does not shed fibers onto the tips, keeping your tips clean and free of paperboard contaminants.

EcoPac® Refills save time & money!

Unsurpassed Clean Release

3ssibio.com

1250 μLFiltered

10 μL P2/P10 Filtered

300 μL Filtered

200 μL

200 μL Filtered

100 μL Filtered

20 μL Filtered

Vertex Pipette Tips

1000 μL Filtered

10 μL P2/P10

10 μL XL

10 μL XL Filtered

300 μL

1000 μL

1250 μL

10 μL P2/P10 Graduated Tips - Graduations at 2 and 10 μL, Natural

4117NSFS 10 µL racked, sterile, filtered 10 racks per unit

4110N00S 10 µL bulk 1000 tips per bag

4117N00S 10 µL racked 10 racks per unit

4117NS0S 10 µL racked, sterile 10 racks per unit

4118N00S 10 µL EcoPac® 20 refill inserts per unit

4118NSFS 10 µL EcoPac®, filtered, sterile 20 refill inserts per unit

10 XL Graduated Tips - Graduations at 2 and 10 μL, Natural

4137NSFS 10 XL racked, sterile, filtered 10 racks per unit

4130N00S 10 XL bulk 1000 tips per bag

4137N00S 10 XL racked 10 racks per unit

4137NSFS 10 XL racked, sterile 10 racks per unit

4138N00S 10 XL EcoPac® 20 refill inserts per unit

4138NSFS 10 XL EcoPac®, sterile, filtered 20 refill inserts per unit

20 μL Graduated Tips - Graduations at 10, 50 and 100 μL, Natural

100 μL Graduated Tips - Graduations at 10, 50 and 100 μL, Natural

200 μL Graduated Tips - Graduations at 10, 50 and 100 μL, Natural

4230N00S 200 µL, bulk 1000 tips per bag

4237N00S 200 µL racked 10 racks per unit

4237NS0S 200 µL racked, sterile 10 racks per unit

4238N00S 200 µL EcoPac® 20 refill inserts per unit

4238NSFS 200 µL EcoPac®, sterile, filtered 20 refill inserts per unit

300 μL Graduated Tips - Graduations at 10, 50, 100 and 200 μL, Natural

4927NSFS 300 µL, racked, sterile, filtered 10 racks per unit

4920N00S 300 µL bulk 1000 tips per bag

4927N00S 300 µL racked 10 racks per unit

4927NS0S 300 µL racked, sterile 10 racks per unit

4928N00S 300 µL EcoPac® 20 refill inserts per unit

4928NSFS 300 µL EcoPac®, sterile, filetered 20 refill inserts per unit

1000 μL Graduated Tips - Graduations at 100, 500 and 1000 μL, Natural

4337NSFS 1000 µL racked, sterile, filtered 10 racks per unit

4330N00S 1000 µL bulk 1000 tips per bag

4337N00S 1000 µL racked 10 racks per unit

4337NS0S 1000 µL racked, sterile 10 racks per unit

4338NSFS 1000 µL EcoPac®, sterile, filtered 20 refill inserts per unit

1250 μL Graduated Tips - Graduations at 100, 500 and 1000 μL, Natural

4347NSFS 1250 µL racked, sterile, filtered 10 racks per unit

4340N00S 1250 µL bulk 1000 tips per bag

4347N00S 1250 µL racked 10 racks per unit

4347NS0S 1250 µL racked, sterile 10 racks per unit

4348N00S 1250 µL EcoPac® 20 refill inserts per unit

4348NSFS 1250 µL EcoPac®, sterile, filtered 20 refill inserts per unit

4237NAFS 20 µL, racked, sterile, filtered 10 racks per unit

4238NAFS 20 µL, EcoPac®, sterile, filtered 20 refill inserts per unit

4237NBFS 100 µL, racked, sterile, filtered 10 racks per unit

4238NBFS EcoPac®, sterile, filtered 20 refill inserts per unit

3

Liquid Handling & PCR Product Guide

ALFAGEN MEDICAL SUPPLIES • Call us: +9-0232-375-25-30; +9-0232-344-25-30 • Mail us: [email protected]

Part Number Skirting Description Compatibility Quantity

3400-00S Unskirted Standard 96-well plate (cuttable) qPCR & PCR box of 10

3420-00S Semi-skirted Standard 96-well plate (flat top) qPCR & PCR box of 10

3425-00S Semi-skirted ABI® shape 96-well plate (raised top ridge) qPCR & PCR box of 10

3426-00S Semi-skirted ABI® style FAST®-type (0.1 ml), Low-profile 96-well plate qPCR box of 10

3450-00S Semi-skirted Standard-shape 96-well plate PCR box of 10

3455-40S Semi-skirted LightCycler® type, Low-profile 96-well plate, white qPCR box of 10

3441-00S Full-skirted Low-profile 96-well plate qPCR & PCR box of 10

3430-00S Full-skirted Two-notch type (A24/P24), 384-well plate qPCR & PCR box of 10

3435-00S Full-skirted One-notch (A24) Type, 384-well plate qPCR & PCR box of 10

UltraFlux® PCR Plates

Unskirted, Standard3400-00S

Unskirted, Low-Profile3401-00S

Semi-Skirted, Standard 3420-00S

Semi-Skirted, ABI Shape3425-00S

Semi-Skirted, ABI Fast3442-00S

Semi-Skirted, Standard 3450-00S

Semi-Skirted, LightCycler®3455-40S

Full-Skirted, Low-Profile3441-00S

Full-Skirted, Two-Notch3430-00S

Full-Skirted, One-Notch3435-00S

SSI offers the largest selection of plates that are designed to precisely fit most thermal cyclers on the market. Each is designed to meet and exceed the specifications and performance of plates supplied by the original equipment manufacturer.

• Well ridges reduce sample evaporation when sealing with film, foil or associated strip caps.• High-contrast black alphanumeric markings for easy well identification.• Ultra-thin, uniform wells ensure optimal heat transfer for high reaction efficiency.• Conveniently packaged in small quantities of 10 plates to minimize risk of contamination.• Certified free from DNase, RNase, DNA, and PCR inhibitors.• Made in the USA

*Also available in colors: blue, green, red, violet, yellow and assorted. See ssibio.com for more details.

5ssibio.com

Support both real-time and standard PCR applications with a variety of plate sealing options. 8-strip and 12-strip caps enable sealing for specific sections while UltraFlux® sealing films are designed for full plate sealing and analysis. Both create evaporation-resistant seals to reduce sample loss.

• Dome and optically-clear flat cap options for qPCR or standard PCR analysis• Sealing films feature removable end tabs for easy handling and application.• Certified free from DNase, RNase, DNA, and PCR inhibitors.• Made in the USA.

Part Number Description Cap Type Compatibility Quantity

3105-00S 8-strip flat caps Flat qPCR & PCR 125 strips of 8

3102-00S 8-strip dome caps Dome PCR 125 strips of 8

3106-00S 12-strip flat caps Flat qPCR & PCR 80 strips of 12

3103-00S 12-strip dome caps Dome PCR 80 strips of 12

3105-00S

3102-00S

3106-00S

3103-00S

Sealing Films & Foils

Use Sealing Film to Reduce Evaporation!

Part Number Description Compatibility Quantity

3651-00S Real-time Sealing Film qPCR & PCR 100 films

3622-00S PCR Sealing Film PCR 100 films

3640-00S Pierceable Aluminum Foil PCR & Storage 100 films

UltraFlux® Plate Sealing

Strip Caps for Plate Sealing

Individual PCR tubes feature evaporation-resistant, easy-to-open/close lids to reduce sample loss and ultra-thin, uniform wells to ensure optimal heat transfer and reaction efficiency.

• Conveniently packaged in re-closable bags to minimize risk of contamination.• Available in ultra-clear, assorted, or specific colors.• Certified free from DNase, RNase, DNA, and PCR inhibitors. • Made in the USA.

Part Number Description Compatibility Quantity

3210-00S 0.2ml dome cap PCR tubes, clear* PCR 1000/unit

3220-00S 0.2ml frosted cap PCR tubes, clear* PCR 1000/unit

3225-00S 0.2ml clear, flat cap PCR tubes, clear qPCR & PCR 1000/unit

3310-00S 0.5ml dome cap PCR tubes, clear PCR 1000/unit

3320-00S 0.5ml frosted cap PCR tubes, clear* PCR 1000/unit

*Also available in colors: blue, green, red, violet, yellow and assorted. See ssibio.com for more details.

UltraFlux® Individual PCR Tubes

Liquid Handling & PCR Product Guide

ALFAGEN MEDICAL SUPPLIES • Call us: +9-0232-375-25-30; +9-0232-344-25-30 • Mail us: [email protected]

NON-SKIRTED, STANDARD0.2ml Rimmed wells

NON-SKIRTED, LOW-PROFILE0.1mlRaised rims

SEMI-SKIRTED, ABI-TYPE0.2ml Fits most cyclers, including ABI Prism® and GeneAmp® Systems.

SEMI-SKIRTED, RAISED RIMS0.2ml

SEMI-SKIRTED, LOW-PROFILE0.1ml LightCycler® compatible

SEMI-SKIRTED, STANDARD0.2ml

FULL-SKIRTED, LOW-PROFILERAISED RIMS0.1ml Stackable Rimmed wells

FULL-SKIRTED, RAISED RIMS, 384-WELL0.2mlConical flat-bottom wells LightCycler® compatible

SEMI-SKIRTED, LOW-PROFILERAISED RIMS0.1ml Fits ABI Fast® cyclers

UltraFlux® Plate Selection Guide

3400-00S A12 corner notch, natural 2/sleeve; 10/box

3400-09S A12 corner notch, assorted colors 2/sleeve; 10/box

3400-40S A12 corner notch, white 2/sleeve; 10/box

3420-00S A12 corner notch, natural 2/sleeve; 10/box

3420-40S A12 corner notch, white 2/sleeve; 10/box

3441-00S A12 corner notch, natural 2/sleeve; 10/box

3442-00S H1 corner notch, white 2/sleeve; 10/box

3426-00S A1 corner notch, natural 2/sleeve; 10/box

33426-40S A1 corner notch, white 2/sleeve; 10/box

3430-00S A24, P24 corner notch, natural 2/sleeve; 10/box

3430-40S A24, P24 corner notch, white 2/sleeve; 10/box

3425-00S A12 corner notch, natural 2/sleeve; 10/box

3450-00S A12 corner notch, natural 2/sleeve; 10/box

3455-40S H12 corner notch, white 2/sleeve; 10/box

3401-00S A12 corner notch, natural 2/sleeve; 10/box

3401-40S A12 corner notch, white 2/sleeve; 10/box

• Rapid, even heat transfer• Real-time compatible• Certified RNase, DNase, DNA, and

PCR inhibitor free• Tested pyrogen-free

• See more styles and colors on our website

• Contact us for free samples!

7ssibio.com

Master

cycle

r® M38

4

Master

cycle

r® ep

Master

cycle

r® Gradien

t

Master

cycle

PTC-20

0/PTC

-220/P

TC-22

5

7300

MyCyc

ler™

7500

“Fas

t”

MyiQ™

7700

IQ5®79

00HT

7900

HT 384

Block

9700

StepOne

Plus™

Verit

ViiA™

7

C1000

™/S

1000

iCycler

®

7500

All trademarks are the property of their respective owner.

LightC

ycler

®

Liquid Handling & PCR Product Guide

ALFAGEN MEDICAL SUPPLIES • Call us: +9-0232-375-25-30; +9-0232-344-25-30 • Mail us: [email protected]

Designed to precisely fit most thermal cyclers on the market today. Strip tubes with white wells enhance fluorescence signal strength, making them ideal for qPCR analysis when combined with an optically-clear flat cap. SnapStrip® tubes are customizable, allowing users to easily split the strip into smaller sections. Combination cap and tube packs are available in standard and low-profile versions and are conveniently packaged in resealable bags.

• Secure, evaporation-resistant seal between cap and tube.• Ultra-thin walls for optimum heat transfer and reaction efficiency.• Optically-clear flat caps – ideal for qPCR and standard PCR.• Crystal-clear clarity – easy to visualize content of each well.• Available in full range of colors or white (enhances qPCR signals).• Certified free from DNase, RNase, DNA, and PCR inhibitors.• Made in the USA.

Part Number Description Compatibility Quantity

3247-00S UltraFlux® 0.2 mL 8-strip tubes with individual flat caps, clear qPCR & PCR 120 strips of 8

3247-40S UltraFlux® 0.2 mL 8-strip tubes with individual flat caps, white tubes qPCR 120 strips of 8

3248-00S UltraFlux® Low Profile 8-strip tubes with individual flat caps, clear qPCR & PCR 120 strips of 8

3248-40S UltraFlux® Low Profile 8-strip tubes with individual flat caps, white tubes qPCR 120 strips of 8

3240-00S SnapStrip® 0.2 mL 8-Strip tubes with individual dome caps, clear PCR 120 strips of 8

3245-00S SnapStrip® 0.2 mL 8-Strip tubes with individual flat caps, clear qPCR & PCR 120 strips of 8

3135-00S Combination Pack: strips of 8 tubes and flat caps, clear qPCR & PCR 125 strips of 8

3135-40S Combination Pack: strips of 8 tubes and flat caps, white tubes qPCR 125 strips of 8

3131-00S Combination Pack: strips of 8 tubes and dome caps, clear PCR 125 strips of 8

3145-00S Combination Pack: Low-Profile strips of 8 tubes and flat caps, clear qPCR & PCR 125 strips of 8

3145-40S Combination Pack: Low-Profile strips of 8 tubes and flat caps, white tubes qPCR 125 strips of 8

3136-00S Combination Pack: strips of 12 tubes and flat caps, clear qPCR & PCR 80 strips of 12

3136-40S Combination Pack: strips of 12 tubes and flat caps, white tubes qPCR 80 strips of 12

3133-00S Combination Pack: strips of 12 tubes and dome caps, clear PCR 80 strips of 12

UltraFlux® tubes feature robust interwell 3-point linkages that make strips more rigid and sturdy.

SnapStrip® easy-to-break single point linkage – use only the tubes you need.

UltraFlux® and SnapStrip® tubes have angularly attached caps, preventing interference from adjacent lids.

UltraFlux Strip Tubes & Caps

9ssibio.com

Microcentrifuge tubes are available in 0.5 mL, 1.5 mL, and 2.0 mL volumes and are solidly constructed for high speed centrifugation. All tubes feature graduations every 100 μL allowing for convenient sample estimation.

• 2 cap styles to fit your preference: - Small boil-proof caps—ideal for rigorous boiling applications and tight storage condition. - Large boil-resistant caps—easy to open & close, with large labeling surface• Rated to 20,000 x g for high-speed centrifugation.• Crystal-clear material allows visualization of hard-to-see pellets.• Available with proprietary NoStick® resin to ensure maximum sample recovery.• Available in ultra-clear, assorted, amber, and specific colors.• Autoclavable.• Certified free from DNase, RNase, DNA, and PCR inhibitors.• Made in the USA.

Part Number Description Max Fill Quantity

1110-00S 0.5 mL, small cap, boil-proof microcentrifuge tube, clear 0.65 mL 1000/pack

1110-10S 0.5 mL, NoStick® small cap, boil-proof microcentrifuge tube, clear 0.65 mL 500/pack

1210-00S 1.5 mL, small cap, boil-proof microcentrifuge tube, clear 1.7 mL 500/pack

1210-10S 1.5 mL, NoStick® small cap, boil-proof microcentrifuge tube, clear 1.7 mL 250/pack

1260-00S 1.5 mL, large cap, boil-resistant microcentrifuge tube, clear 1.7 mL 500/pack

1310-00 2 mL, small cap, boil-proof microcentrifuge tube, clear 2.0 mL 500/pack

Microcentrifuge Tubes

• Fits all rotors and racks that hold 15 mL conical tubes - no adapters needed.• Graduated every 250 μL allows for easy volume estimation.• Rated to 25,000 x g for high-speed centrifugation.• Assorted colors come in 4 individually packaged colors (red, blue, green, yellow).• Autoclavable.• Certified free from DNase, RNase, DNA, and PCR inhibitors.• Made in the USA.

Part Number Description Quantity

1410-00S 5 mL SnapCap centrifuge tube, clear 100/bag; 2 bags/pack

1410-09S 5 mL SnapCap centrifuge tube, assorted colors (individually packed) 50/bag; 4 bags/pack

5415-59S 5 mL tube-compatible Flipper® rack 50/bag; 4 bags/pack

New 5 mL Flipper® rack!Holds 0.5, 1.5, 2, 5, 15 mL, and 50 mL tubes

5 mL SnapCap Microcentrifuge Tubes

Liquid Handling & PCR Product Guide

ALFAGEN MEDICAL SUPPLIES • Call us: +9-0232-375-25-30; +9-0232-344-25-30 • Mail us: [email protected]

One side can hold up to (20) 1.5 mL or 2 mL tubes, the other side can hold up to (20) 0.5 mL tubes.

• Blue -20°C unit will maintain a temperature between -10°C and -20°C for up to 3 hours• White 0°C unit will maintain a temperature of 0°C for a minimum of five hours• Constructed of polycarbonate material• Made in the USA.

Part Number Description Capacity Quantity

5610-43S IsoFreeze® Flipper® rack for tubes, Blue -20°C unit (20) 0.5 mL, 1.5 mL or 2 mL tubes 1 rack per pack

5610-40S IsoFreeze® Flipper® rack for tubes, White 0°C unit (20) 0.5 mL, 1.5 mL or 2 mL tubes 1 rack per pack

IsoFreeze® Flipper® Racks

5230-29S5520-29S

Part Number Description Capacity Quantity

5520-29SS Workup rack and workstation unit with lid

Unit: (32) 0.2 mL tubes, (24) 1.5 or 2 mL tubes, (16) 0.5 mL tubesRack: (1) 96 well plate or (96) 0.2ml tubes or (12) 8-strip tubes

10 per pack

5230-29S Workup rack with lid (1) 96 well plate or (96) 0.2 mL tubes or (12) 8-strip tubes 5 per pack

5240-09S Stacking Workup rack with lid (1) 96 well plate or (96) 0.2 mL tubes or (12) 8-strip tubes 5 per pack

Versatile racks are perfect for all pre- and post-PCR sampling applications. Available in a variety of colors, each rack can support a wide range of tube sizes and plates and is safe to -80°C.

PCR Work Up Racks

5240-09S

11ssibio.com

Leave the ice buckets behind! IsoFreeze® racks contain an inert, non-toxic, color-changing gel that maintains a temperature of less than 4°C for almost 4 hours (with lid on rack) at room temperature. Upon reaching 7°C, the rack alerts the user by undergoing a distinct color change from green to yellow or purple to pink. An ideal combination of function and utility, these racks can go from freezer to lab bench without having to transfer samples.

5630-T6S 5640-T4S 5650-T6S

Part Number Description Capacity Quantity

5630-T4S IsoFreeze® rack for tubes, purple to pink color change (24) 0.5, 1.5 or 2 mL tubes 1 rack per pack

5630-T6S IsoFreeze® rack for tubes, green to yellow color change (24) 0.5, 1.5 or 2 mL tubes 1 rack per pack

5640-T4S IsoFreeze® rack for PCR, purple to pink color change(1) 96 well plate or (96) 0.1 or 0.2 mL tubes or (12) 8-strip tubes

2 racks per pack

5640-T6S IsoFreeze® rack for PCR, green to yellow color change(1) 96 well plate or (96) 0.1 or 0.2 mL tubes or (12) 8-strip tubes

2 racks per pack

5650-T4S IsoFreeze® rack for PCR, SBS footprint, purple to pink color change(1) 96 well plate or (96) 0.1 or 0.2 mL tubes or (12) 8-strip tubes

2 racks per pack

5650-T6S IsoFreeze® rack for PCR, SBS footprint, green to yellow color change(1) 96 well plate or (96) 0.1 mL or 0.2 mL tubes or (12) 8-strip tubes

2 racks per pack

IsoFreeze® Racks

Scientific SpecialtiesInnovations for Life Science

UltraFlux®, SnapStrip®, NoStick®, IsoFreeze®, Flipper® are trademarks or registered trademarks of Scientific Specialties, Inc.All other are trademarks or registered trademarks ar the property of their respective manufacturers, distributors, or agents.

• Call us : +9-0232-375-25-30; +9-0232-344-25-30

• Mail us : [email protected]

Call us: +9-0232-375-25-30; +9-0232-344-25-30Mail us: [email protected]