lignin utilization wbs 2.3.4 - energy.gov · 2015-04-20 · 2 goal statement goal: develop viable...
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NREL is a national laboratory of the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, operated by the Alliance for Sustainable Energy, LLC.
Lignin Utilization WBS 2.3.4.100
2015 DOE BioEnergy Technologies Office (BETO) Project Peer Review
Date: March 24th, 2015
Technology Area Review: Biochemical Conversion
Principal Investigator: Gregg T. Beckham
Organization: National Renewable Energy Laboratory
This presentation does not contain any proprietary, confidential, or otherwise restricted information
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Goal Statement Goal: develop viable processes to produce valuable co-products from lignin • Contribute to 2022 cost targets in Biological Conversion and Catalytic Upgrading of Sugars to
HCs
• Focus on products with sufficient market size and growth potential to aid bioenergy industry
Lignin utilization will be a major benefit to the US biorefinery infrastructure • Conduct TEA/LCA to identify cost drivers and data gaps, and to refine process options • Collaborate with industry and academics for development of tangible lignin utilization processes • Outcome: demonstrated, scalable processes for converting lignin to valuable co-products
Lignin Depolymerization • Obtain sufficient lignin in liquid phase • Understand impact to carbohydrates
Lignin Upgrading • New upgrading process represent novel
foundation for lignin utilization • Leverage new/existing deconstruction processes
Lignin Depolymerization • Obtain sufficient lignin in liquid phase • Understand impact to carbohydrates
Lignin Upgrading • New upgrading process represent novel
foundation for lignin utilization • Leverage new/existing deconstruction processes
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Quad Chart
• Start date: October 2012 • End date: September 2017 • Percent complete: 50%
• Bt-D Pretreatment Processing and Selectivity
• Bt-I Catalyst Efficiency • Bt-J Biochemical Conversion Process
Integration
Timeline
Budget
Barriers
• Industry partners: Shell Global Solutions (CRADA), POET (Work-for-Others), RJ Reynold Tobacco Company (CRADA), CRB Innovations, in discussions with several other companies regarding lignin utilization
• NREL BETO Projects: Biochemical Platform Analysis, Feedstock Process Interface, Pretreatment and Process Hydrolysis, Biological Lignin Depolymerization, Biological Pyrolysis Oil Upgrading, Pilot Scale Integration, Biochemical Process Modeling and Simulation, Enzyme Engineering and Optimization, Strategic Analysis Platform
• BETO-funded National Lab Projects: Oak Ridge National Laboratory (A. Guss), Idaho National Laboratory (A. Ray), Sandia National Laboratory, Joint BioEnergy Institute (J. Gladden, B. Simmons)
• Office of Science funded efforts: BioEnergy Science Center, C3Bio (EFRC), Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory (R. Robinson, E. Zink)
• Academic collaborators: University of Georgia, Iowa State University, University of Illinois UC, Colorado School of Mines, University of Oxford, Northwestern University, Purdue University, Swedish University of Agricultural Sciences, University of Portsmouth, University of Tennessee Knoxville
Partners and Collaborators
FY13 Costs FY14 Costs
Total Planned Funding
(FY15-Project End Date)
DOE funded $886,109 $1,172,790 $3,841,102
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Project Overview
Context: Lignin is a primary biomass polymer • ~15-30% of biomass • ~40% of biomass carbon • Typically slated for heat & power • Lignin valorization essential for DOE BETO
$3/gge cost target by 2022 (-$1.89/gge)
Project Objectives: - Develop industrially-relevant pathway(s) for lignin isolation and upgrading to meet 2022
cost targets - Conduct integrated process development with carbohydrate utilization projects - Employ TEA/LCA to develop process options based on bench-scale data
Davis et al., NREL Design Report, 2014
History: Lignin valorization has a very long history, restarted at NREL in ~2012 • BETO seed project on lignin depolymerization catalysis • Identified as a major cost driver for HC biofuels in the National
Advanced Biofuels Consortium
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Technical Approach
Approach: • Obtain lignin in pretreatment and/or post-EH
residual solids • Develop lignin depolymerization strategies
Primary challenges and success factors: • High yields of stable, liquid, low MW products • Analysis of resulting product distributions • Impact to both carbohydrates and lignin in
pretreatment Ragauskas, Beckham, Biddy, et al., Science, 2014
Aim 1: Obtain liquid-phase intermediates
Linger, Vardon, Guarnieri, Karp, et al., PNAS 2014 Vardon, Franden, Johnson, Karp, et al., EES 2015
Approach: • “Biological Funneling” to obtain single products • Employ separations and chemical catalysis (if
needed) to obtain final target molecule
Primary challenges and success factors: • Achieve high yields in the biological step • Co-design of lignin stream, organism, and
target product • Cost-effective separations
Aim 2: Use biology & catalysis to upgrade lignin
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Management Approach
Aim 1: Obtain liquid-phase intermediates FY13: Evaluation of multiple pretreatments FY14: Stream characterization for upgrading, evaluation of depolymerization catalysts FY15: Finalize pretreatment and catalysis
• Develop simple, integrated approaches and use TEA/LCA and Go/No-Go’s to refine options • Employ fundamentals-driven science/engineering approach with an interdisciplinary team
Aim 2: Use biology & catalysis to upgrade lignin FY13: Proof of concept for lignin upgrading FY14: Conduct co-product modeling to identify targets FY15: Down-select and improve upgrading organisms
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Technical Results – Outline Pretreatment to obtain lignin-enriched streams • Clean Fractionation (Organosolv) • Alkaline pretreatment • Alkaline peroxide pretreatment • Ammonia pretreatment • Deacetylation/Mechanical Refining
(Collaboration with PPH)
Lignin-Centric Post-treatments to obtain depolymerized streams • Homogeneous base catalysis • Heterogeneous base catalysis • Heterogeneous metal catalysis (reductive and
oxidative) • Focus on biorefinery-relevant substrates for
depolymerization catalysis
Lignin Upgrading to produce valuable products • Biological funneling • Separations research • Catalysis for product upgrading • Focus on biorefinery-relevant
substrates
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Lignin Isolation via Alkaline Pretreatment Comprehensive TEA-centric approach using NaOH to deconstruct lignin • Full mass balances, full characterization of resulting lignin streams • Cellulose digestibility with industrial enzyme cocktails • Bench-scale data for TEA/LCA including Materials of Construction and reactor costs
Karp et al., ACS Sust. Chem. Eng., 2014 Karp et al., in review, 2015
Corn Stover Lignin Yield Switchgrass Lignin Yield Objective Function • Work ongoing with
blended/pelleted feedstocks with INL, NREL FI Tasks
• Also with C3Bio GM poplar feedstocks (high S lignin)
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Alkaline Pretreatment at Pilot Scale Currently conducting alkaline pretreatment in pilot-scale batch reactors • First 1900-L run was conducted at 7% solids loadings (same as deacetylation) • Optimal condition defined from bench-scale runs
Linger, Vardon, Guarnieri, Karp, et al., PNAS 2014
Transitioning to continuous, high-solids pretreatment
Complex product distribution warrants continued method development
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Alternative pretreatments Organosolv Pretreatment • H2O/EtOH/MIBK + H2SO4
• H2O/Acetone/MIBK + H2SO4
• TEA dictated omission of this pathway
Katahira et al., ACS Sust. Chem. Eng., 2014 Resch et al., ACS Sust. Chem. Eng., 2014
Acetone Organosolv Solid Mass Balances
Acetone Organosolv Enzymatic Digestions
Alkaline Peroxide Pretreatment • H2O2, NaOH, pH ~11.5, T=[25-80 C] • Analysis on liquor and preliminary TEA ongoing
Deacetylation/Mechanical Refining Pretreatment • Characterizing residual lignin after pretreatment and
enzymatic hydrolysis with other NREL BETO projects • Material for Biol. Lignin Depolymerization (WBS 2.3.2.100) • Examining catalytic depolymerization of residual lignin
0%
20%
40%
60%
80%
100%
Raw CS 0.03 0.06 0.09 0.13
Frac
tion
in R
esid
ual S
olid
s, %
of i
nitia
l
H2O2 Loading (g / g Dry Corn Stover)
Glucan Xylan Lignin
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Homogeneous Base Catalysis (Base-Catalyzed Depolymerization)
• Dilute Acid Pretreated, Enzymatically Hydrolyzed (DAP-EH) lignin
• Deacetylated/Mechanical-Refined, Enzymatically Hydrolyzed (DMR-EH) lignin
Substrate Temp ( C)
NaOH, %
Solid loading, %
DAP-EH
DMR-EH
330 4 2 10
300 4 2 10
270 4 2 10
45 53 47 47
57 55 59 60 57 60 70 69
0
10
20
30
40
50
60
70
80
270˚C 300˚C 330˚C 270˚C 300˚C 330˚C
2% NaOH 4% NaOH Yi
eld
(wt%
)
Yield of aqueous fraction DAP-EH DDR-EH DAP-EH DMR-EH
BCD is an effective process • Achieve > 60% liquid yields • DDR-EH yields more lignin-
derived species than DAP-EH • GPC reveals low MW distributions • TEA and upgrading experiments
for BCD liquors ongoing currently • Examining blended/pelleted
feedstocks with INL, NREL FI
Katahira, Mittal et al., in review
Liquor after BCD
Solid residue after BCD
DMR-EH
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Heterogeneous Base Catalysis Sturgeon et al., Green Chem. 2014 Kruger et al., in review
Hydrotalcite materials can act as heterogeneous base catalysts • Effective catalysts for lignin depolymerization • More recently, interested in catalyst regeneration and recycling
NO3- crucial for activity
Nitrated-HTC is an effective catalyst for lignin depolymerization • Requires no transition metals or external H2
• Catalyst regeneration possible via nitration • Worked with Shell Global Solutions to
develop catalyst technology • Transitioning technology to industry partners • Discontinuing this work as a major focus in
the project to focus on scale-up of BCD
Enables catalyst regeneration
HTC-NO3 as effective as the original Ni-HTC
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Summary of Lignin Deconstruction and Isolation Lignin-Centric Pretreatment to produce liquors for upgrading • Alkaline pretreatment � • Organosolv pretreatment � • Alk. peroxide pretreatment (TEA ongoing)
Lignin-Centric Post-treatment to produce liquors for upgrading • Base-catalyzed depolymerization � • Hydrotalcite-catalyzed depolymerization �
• Examine both DAP-EH and DDR-EH pretreated substrates to maintain relevance to cost target demonstrations
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Lignin Upgrading
Primary challenge in lignin valorization to co-products is heterogeneity Significant need for innovation to overcome problems with heterogeneous depolymerization products regardless of depolymerization strategy
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Biological Funneling Linger, Vardon, Guarnieri, Karp et al., PNAS, 2014
Biological Funneling Cultivations on APL
Biological Funneling enables conversion of lignin-derived aromatics into value-added compounds • Significant versatility in lignin stream, organism, and
target product • Demonstrated mcl-PHA production in Pseudomonas
putida KT2440 on alkaline pretreated liquor • Higher-solids pretreatment will enable more efficient
biological conversion step • Examining mcl-PHA separation and cleanup strategies • Working with Adam Guss at ORNL and John Gladden
at SNL for improvements via metabolic engineering
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Adipic acid production from lignin Vardon, Franden, Johnson, Karp et al., Energy Env. Sci., 2015
Adipic acid identified as a primary target from lignin from a TEA and LCA perspective • Combined metabolic engineering, fermentation,
separations, and catalysis • Demonstrated muconic acid production in engineered
P. putida KT2440 on alkaline pretreated liquor • Purity is a key cost driver; examining separations
options and downstream polymer properties
Separations
Catalysis
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Alkaline Pretreatment/BioFunneling TEA Preliminary TEA shows a pathway to $3/gge with lignin utilization • Alkaline pretreatment conditions based on pilot-scale runs • Key data gaps (and cost drivers) are carbon efficiency/yield to products and separations • TEA being used to focus bench-scale research Adipic acid case
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Relevance Lignin utilization will be essential to achieve 2017 (potential) and 2022 (definite) HC fuel cost targets Highlighted in 2011 Review/CTAB and 2014 PRINCE Workshop as a key gap in BC Platform Key MYPP areas for process improvement via lignin utilization:
Pretreatment Processing/Selectivity - Developing lignin-centric
pretreatments - Obtain lignin-enriched streams
for depolymerization/upgrading
Biochem. Conv. Process Integration - Working directly with other
BETO-funded tasks in lignin isolation and scale-up
- E.g., DMR-EH depolymerization
Catalyst Efficiency - Developing chemical catalysts
for lignin depolymerization and biocatalysts for lignin upgrading
Key Stakeholders and Impacts:
- Industrial and academic research focused on carbohydrate utilization can leverage lignin utilization processes
- Work will enable adoption of lignin utilization in modern biorefinery designs
- Lignin impacts the “Whole Barrel of Oil” initiative
- Portfolio of chemicals from lignin will diversify and accelerate development of the biomass value chain
- Significant amounts of peer-reviewed science and IP will be generated from this work (14 papers)
- Methods to upgrade heterogeneous intermediates can be adapted by the Bio-Oil Platform
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Future Work Lignin Isolation/Deconstruction • Define conditions by end of FY15 for viable isolation efforts on DAP-EH and DDR-EH
feedstocks, continue to work with INL and NREL tasks to adapt to different feedstocks • Transition isolation/deconstruction efforts to single technologies only, ramp up integration
efforts with upgrading
Lignin Upgrading • Working towards intracellular carbon storage products (e.g., PHAs and fatty acids) and
secreted products (e.g., adipic acid) • Emphasizing upgrading and integration R&D, including collaborations with other BETO projects
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Summary 1) Approach:
– obtain and upgrade lignin to chemicals and/or fuels with fundamentals-driven, interdisciplinary approach underpinned by TEA and LCA
– collaborate widely with academic, national lab, and industrial partners including BC Platform tasks
2) Technical accomplishments – demonstrated high yields of liquid products from BCD on process-relevant feedstocks – defined a wide range of process conditions for alkaline pretreatment on multiple feedstocks – developed a novel, integrated upgrading approach to overcome lignin heterogeneity by coupling
biology, separations, and catalysis – key to lignin utilization – demonstrated Biological Funneling concept for PHA and adipic acid production from lignin to date
3) Relevance – co-products essential to meet DOE hydrocarbon cost targets – addresses Whole Barrel of Oil Initiative and bolsters the biomass value chain
4) Critical success factors and challenges – heterogeneity, economic and sustainable production of co-products, high yields of products needed
5) Future work: – finalize conditions for isolation/deconstruction of lignin by end of FY15 – ramp up efforts on lignin upgrading as standalone process and co-utilization with carbohydrates
6) Technology transfer: – working with multiple industry partners to build commercialization path to lignin utilization – direct and functional replacement chemicals from biomass
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Acknowledgements • Mary Biddy • Stuart Black • Adam Bratis • Steve Chmely • Peter Ciesielski • Ryan Davis • Bryon Donohoe • Rick Elander • Mary Ann Franden • Michael Guarnieri • Jessica Hamlin • Chris Johnson • David Johnson • Rui Katahira • Eric Karp • Jake Kruger
• Eric Kuhn • Kelsey Lawrence • Jeffrey Linger • Lauren Magnusson • Kellene McKinney • Bill Michener • Ashutosh Mittal • Marykate O’Brien • Phil Pienkos • Heidi Pilath • Michael Resch • Davinia Salvachua • Dan Schell • Matthew Sturgeon • Derek Vardon
External collaborators
• Adam Guss, Oak Ridge National Laboratory
• Allison Ray, Idaho National Laboratory
• Ellen Neidle, University of Georgia
• Robert Brown, Laura Jarboe, Marjorie Rover, Ryan Smith, Xianglan Bai, Iowa State University
• R. Robinson, E. Zink, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
• Alison Buchan, University of Tennessee Knoxville
• John McGeehan, Simon Cragg, University of Portsmouth
• Jerry Ståhlberg, Mats Sandgren, Swedish University of Agricultural Sciences
• Linda Broadbelt, Northwestern University
• Mahdi Abu-Omar, Clint Chapple, Rick Meilan, Purdue University
• Timm Strathmann, UIUC, CSM
• Rob Paton, University of Oxford
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Additional slides
• Previous reviewer comments from 2011 Peer Review related to lignin
• Previous reviewer comments from 2013 Peer Review on Lignin Utilization Project
• Publications • Patents • Presentations • Acronyms
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Previous reviewer comments regarding lignin (2011)
Lignin Utilization is a new task, but herein are included comments from the 2011 reviews on the Biochemical Conversion platform related to lignin.
• “Expansion of the approach to lignin might be worth some analysis as it
is a quite significant fraction of the total cellulosic biomass available.”
• “Lignin is a major component of biomass, yet it is given relatively little attention in the Platform work (other than its negative impact on saccharification). This is undoubtedly due to the difficulty in making liquid fuels from lignin, especially via a biochemical process. Our perception is that it is reasonable to begin to expand the work directed at finding value-added uses for lignin (there is one such international project). If successful in this effort, then it seems this would have a major impact on the cost of converting the carbohydrate component to ethanol.”
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Previous reviewer comments regarding Lignin Utilization
Are there any other gaps in the portfolio for this technology area? Are there topics that are not being adequately addressed? Are there other areas that BETO should consider funding to meet overall programmatic goals? “Funding for both the biological and the catalytic pathway should include FOAs for higher-value products that will enhance the economic viability of the hydrocarbon platform. Lignin utilization is a key co-product that needs continued research.”
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• “Excellent project! Very impressive with great progress in less than a year.”
• “Overall, this is a strong project that addresses one of the key hurdles to achieve the 2017 and 2022 MYPP goals; namely the utilization of lignin to create value-added products to support replacement of the whole barrel of oil and achieving the $3/gal metric. This project is a good example of the application of fundamentals-driven science and techno-economic analyses.”
• “Seems like a great project; it is too early to see where this will end up but it has promise. How will this be different from other lignin upgrading efforts? PIs need to keep an eye on development of low-lignin transgenic plants.”
• “This is a very strong project. The highest and best lignin utilization is an essential component of bioenergy and biochemical production. As the PI mentions, lignin heterogeneity is a major hurdle.”
Previous reviewer comments regarding Lignin Utilization
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• “This project tackles a very difficult technical challenge that has been studied extensively. The outcome of using lignin and taking to intermediates to make higher-value chemicals could be significant.”
• “This is a very relevant and important project that allows low-cost feedstock for fuels and chemicals.”
PI Response to Reviewer Comments: • We thank the reviewers for their positive comments. Regarding the work
on plants with genetically modified lignin, we are currently collaborating with several groups on this topic to understand the influence of less recalcitrant lignins on the lignin removal, deconstruction, and upgrading. The genetically modified lignin feedstocks may offer a dramatic reduction in processing costs and simplify lignin upgrading processes, which certainly could be a revolutionary change in biomass conversion.
Previous reviewer comments regarding Lignin Utilization
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Publications Publications in review: 1. J.S. Kruger, N. Cleveland, T. Lammens, P.G. Hamilton, M.J. Biddy, G.T. Beckham, “Nitrate-
Intercalated Hydrotalcites for β-O-4 Ether Bond Cleavage”, in review. 2. R. Katahira, A. Mittal, K. McKinney, G.T. Beckham, “Base-catalyzed depolymerization of
lignin-enriched residual substrates from biochemical conversion”, in review. 3. E.M. Karp, M.G. Resch, B.S. Donohoe, P.N. Ciesielski, M.H. O’Brien, J.E. Nill, A. Mittal, M.J.
Biddy, G.T. Beckham, “Alkaline pretreatment of switchgrass”, in review. 4. A.W. Pelzer, M.R. Sturgeon, A.J. Yanez, G. Chupka, M.H. O’Brien, R. Katahira, R.D.
Cortright, L. Woods, G.T. Beckham, L.J. Broadbelt, “Acidolysis of α-O-4 aryl-ether bonds in lignin model compounds: A modeling and experimental study”, in revision.
Publications in print: 4. C.W. Johnson, G.T. Beckham, “Aromatic catabolic pathway selection for optimal production
of pyruvate and lactate from lignin”, in press at Metabolic Engineering. 5. D.R. Vardon, M.A. Franden, C.W. Johnson, E.M. Karp, M.T. Guarnieri, J.G. Linger, M.A.
Salm, T.J. Strathmann, G.T. Beckham, “Adipic acid production from lignin”, in press at Energy and Environmental Science.
6. J.G. Linger‡, D.R. Vardon‡, M.T. Guarnieri‡, E.M. Karp‡, G.B. Hunsinger, M.A. Franden, C.W. Johnson, T.J. Strathmann, P.T. Pienkos, G.T. Beckham, “Lignin valorization through integrated biological funneling and chemical catalysis”, Proc. Natl. Acad. Sci. (2014), 111(33), pp. 12013-12018.
7. A. Ragauskas, G.T. Beckham, M.J. Biddy, R. Chandra, F. Chen, M.F. Davis, B.H. Davison, R.A. Dixon, P. Gilna, M. Keller, P. Langan, A.K. Naskar, J.N. Saddler, T.J. Tschaplinski, G.A. Tuskan, C.E. Wyman, “Lignin Valorization: Improving Lignin Processing in the Biorefinery”, Science (2014), 344, 1246843.
8. E.M. Karp, B.S. Donohoe, M.H. O’Brien, P.N. Ciesielski, A. Mittal, M.J. Biddy, G.T. Beckham, “Alkaline pretreatment of corn stover: Bench-scale fractionation and stream characterization”, ACS Sust. Chem. Eng. (2014), 2(6), pp. 1481-1491, featured on cover.
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Publications Publications in print: 9. M.G. Resch, B.S. Donohoe, P.N. Ciesielski, J.E. Nill, L. Magnusson, M.E.
Himmel, A. Mittal, R. Katahira, M.J. Biddy, G.T. Beckham, “Clean fractionation pretreatment reduces enzyme loadings for biomass saccharification and reveals the mechanism of free and cellulosomal enzyme synergy“, ACS Sust. Chem. Eng. (2014), 2(6), pp. 1377-1387.
10. R. Katahira, K. McKinney, A. Mittal, P.N. Ciesielski, B.S. Donohoe, S. Black, D.K. Johnson, M.J. Biddy, G.T. Beckham, “Evaluation of clean fractionation pretreatment for the production of renewable fuels and chemicals from corn stover“, ACS Sust. Chem. Eng. (2014), 2(6), pp. 1364-1376.
11. M.R. Sturgeon‡, S. Kim‡, K. Lawrence, R.S. Paton, S.C. Chmely, M.R. Nimlos, T.D. Foust, G.T. Beckham, “An experimental and theoretical study of acid catalysis of aryl-ether linkages: Implications for lignin acidolysis”, ACS Sustain. Chem. Eng. (2014), 2(3), pp. 472-485.
12. M.R. Sturgeon, M.H. O’Brien, P.N. Ciesielski, J. Kruger, S.C. Chmely, R. Katahira, J. Hamlin, K. Lawrence, T.D. Foust, R.M Baldwin, M.J. Biddy, G.T. Beckham, “Lignin depolymerization by nickel supported layered double hydroxide catalysts”, Green Chem. (2014), 16, pp. 824-835.
13. R. Davis, L. Tao, E. Tan, M. Biddy, G.T. Beckham, C. Scarlata, J. Jacobson, K. Cafferty, J. Ross, J. Lukas, D. Knorr, P. Schoen, “Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid Prehydrolysis and Enzymatic Hydrolysis Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons”, NREL Report, (2013).
14. S.C. Chmely, S. Kim, P.N. Ciesielski, G. Jiminez-Oses, R.S. Paton, G.T. Beckham, “Mechanistic study of a Ru-xantphos catalyst for tandem alcohol dehydrogenation and reductive aryl-ether cleavage”, ACS Catalysis 2013.
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Patents Patents: • Base-catalyzed depolymerization of lignin with heterogeneous catalysts, application 61/710,240 filed on 10/5/2012
• Lignin conversion to fuels, chemicals, and materials, application 14/563,299 filed on 12/8/2014
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Presentations 1. Valorization of lignin to renewable fuels and chemicals through biological funneling and chemical catalysis, Spring 2015 American Chemical Society
Meeting, March 22, 2015 2. Adipic acid production from lignin, Spring 2015 American Chemical Society Meeting, March 22, 2015 3. Breaking down plant cell walls: A few short stories in improving biofuels production processes, West Virginia University, January 16, 2014 4. Lignin valorization via biological funneling and chemical catalysis, Iowa State University, December 16, 2014 5. Layered Double-Hydroxide Catalysts for Lignin Depolymerization, AIChE Fall meeting, November 17, 2014 6. Breaking down plant cell walls: A few short stories in improving biofuels production processes, West Virginia University, November 14, 2014 7. Breaking down plant cell walls: A few short stories in improving biofuels production processes, Northwestern University, October 30, 2014 8. Lignin valorization via biological funneling and chemical catalysis, Frontiers in Biorefining, October 23, 2014 9. Optimizing biological funneling of lignin and carbohydrate-derived species: substrate depolymerization, organism selection, and co-product
generation, Frontiers in Biorefining, October 22, 2014 10. Breaking down plant cell walls: A few short stories in improving biofuels production processes, Colorado College, October 17, 2014 11. Breaking down plant cell walls: A few short stories in improving biofuels production processes, UC Denver, September 19, 2014 12. Revealing differences between free and complexed enzyme mechanisms and factors contributing to cell wall recalcitrance, 10th European
Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses, Lille, France, September, 12, 2014 13. Active site dynamics in metal/hydrotalcite-catalyzed lignin depolymerization. ACS Fall Meeting, August 14 2014 14. Integrated catalytic upgrading of muconic acid to adipic acid for renewable nylon precursor production, Fall 2014 American Chemical Society
Meeting, August 12, 2014 15. Metal-hydrotalcite solid catalysts for base-catalyzed depolymerization of lignin, ACS Fall Meeting, August 12 2014 16. Conversion of lignin to fuels and chemicals using catalysis and biology, SIMB Annual Meeting, July 21, 2014 17. Directing carbon flow though catechol and protocatechuate pathways for increased yield in biological lignin upgrading, SIMB Annual Meeting, July
21, 2014 18. Understanding free and complexed enzyme mechanisms and factors contributing to cell wall recalcitrance, SIMB Annual Meeting, July 20, 2014 19. Integrated deconstruction, biological upgrading, and chemical catalysis for lignin valorization, Hybrid Processing for Biorenewable Fuels and
Chemicals Production Symposium, Iowa State University, July 11, 2014 20. Clean fractionation of cell wall components in corn stover and their characterization for biorefinery processes, 36th SBFC , April 28, 2014 21. Understanding natural mechanisms of cellulose (and lignin) deconstruction, Joint BioEnergy Institute, April 23, 2014 22. Understanding natural mechanisms of cellulose (and lignin) deconstruction, Energy Biosciences Institute, UC Berkeley, April 22, 2014 23. Characterization of clean fractionated cell wall components in corn stover, 35th SBFC , April 29, 2013 24. Bench-scale process development for carbohydrate and lignin co-utilization, C2B2 Annual Meeting, April 5, 2013
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Acronyms
• BCD: Base-Catalyzed Depolymerization • CF: Clean Fractionation • CHP: Combined Heat and Power • DAP-EH: Dilute-Acid Pretreated, Enzymatically Hydrolyzed
feedstock • DDR-EH: Deacetylated, Disk-Refined, Enzymatically Hydrolyzed
feedstock • LCA: Life-Cycle Analysis • MW: Molecular Weight • TEA: Techno-Economic Analysis