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Libyan International Medical University 2nd Year First Semester D Caroline Piske de A. Mohamed Slide 2 Objectives Learning issues and objectives: What is meant by periodontium? What are its various components? Describe the functions of periodontium and how they are performed. Slide 3 Slide 4 Slide 5 Slide 6 Slide 7 PERIODONTIUM Cementum PDL Alveolar bone Sharpey's fibers Attachment organ Cementum Periodontal ligament Alveolar bone Apical foramen Pulp cavity Enamel Dentin Gingiva Root canal Alveolar vessels & nerves Slide 8 Slide 9 Slide 10 FUNCTIONS OF PDL SUPPORT: PDL supports teeth in their socket. It prevents loosening of teeth. MASTICATORY LOAD: PDL permits teeth to withstand the considerable forces of mastication. SENSORY: PDL is supplied by abundant receptors and nerves that sense the movement when teeth are in function. Helps in the proper positioning of the jaws during normal function. Slide 11 NUTRITIVE: Blood vessels of ligament provide essential nutrients for the ligaments vitality and hard tissue of cementum and alveolar bone. Fibroblasts, osteoblasts, cementoblasts, and even resorptive osteoclasts and macrophages require nutrition. Slide 12 CLINICAL CORRELATION: Bone, PDL, and the cementum together form a functional unit of special importance when the orthodontic tooth movement is undertaken. Orthodontic forces causes compression and constriction of blood vessels, soft tissue changes occur. Hence loss of alveolar bone occurs, now blood flow occurs in the spaces. And the mesenchymal cells of PDL repair the tissues. Slide 13 MAINTAINENCE: Tissues are maintained under the influence of heavy masticatory forces. ADAPTIVE ROLE SHOCK ABSORBER: It absorbs the shock of chewing. Slide 14 PRINCIPAL FIBER BUNDLES OF PDL 1. THE ALVEOLAR CREST GROUP These are attached to the cementum just below the cementoenamel junction and running downward and outward to insert into the rim of the alveolus. Slide 15 2.THE HORIZONTAL GROUP These are just apical to the alveolar crest fibers and running at right angles to the long axis of the tooth from the cementum to the bone below the alveolar crest. Slide 16 3. THE OBLIQUE GROUP They are the most numerous in the PDL and running from the cementum in an oblique direction to insert into the bone coronally Slide 17 4.THE APICAL GROUP: These are radiating from the cementum around the apex of the root to the bone forming the base of the socket. Slide 18 5.THE INTERADICULAR GROUP Found only in the multi-rooted teeth and running from the cementum into the bone forming the crest of the Interradicular septum. Slide 19 TEETH IN-SITU Slide 20 Slide 21 Slide 22 Slide 23 Slide 24 Slide 25 Slide 26 Slide 27 Slide 28 Slide 29 Slide 30 Slide 31 TYPES OF CEMENTUM CEMENTUM is classified according to the presence or absence of cells within its matrix. CELLULAR CEMENTUM, which has an adaptive role in response to tooth wear and movement and is associated with repair of periodontal disease. ACELLULAR CEMENTUM, which provides attachment for the tooth. A- CELLULAR CEMENTUM B-ACELLULAR CEMENTUM Slide 32 Slide 33 Slide 34 Slide 35 Slide 36 Slide 37 Slide 38 Slide 39 Slide 40 Slide 41 Slide 42 Slide 43 Slide 44 Slide 45 Slide 46 Slide 47 Slide 48 Slide 49 Materia alba Bacterial aggregations, leucocytes and desquamated oral epithelial cells accumulating at the surface of plaque and teeth, but lacking the regular internal structure observed in dental plaque. ( mouth rinse can desegregate it) D Caroline Mohamed 49 Slide 50 Slide 51 Dental plaque Dental plaque is the soft, nonmineralized bacterial deposit which forms on teeth and dental protheses that are not adequately cleaned. Le 1965 Microscopically, plaque is simply confluent colonies of microorganisms connected by a matrix consisting predominantly of bacterial and salivary polymers. These accumulations subject the teeth and gingival tissues to high concentrations of bacterial metabolites, which result in dental disease. The dominant bacterial species in dental plaque are Streptococcus sanguis and Streptococcus mutans, both of which are considered responsible for plaque. D Caroline Mohamed51 Slide 52 Differences between materia alba, plaque and calculus D Caroline Mohamed 52 Slide 53 Major sites of plaque accumulation Fissures of molar teeth Supragingival: on the tooth surface above the gingiva Subgingival: in the area bounded by the margin of the gum and the tooth Interproximal: between adjacent teeth Slide 54 Plaque formation Slide 55 Plaque formation is initiated by a weak attachment of the streptococcal cells to salivary glycoproteins forming a pellicle on the surface of the teeth. Bacteria adhere to the pellicle, and pellicle coats the enamel. The specific type of bacteria/pellicle adherence is determined by the innate characteristic of the bacteria and the pellicle. Gram positive rods and cocci are laid down in the first hour. Time elapsed 1 hour Slide 56 The initial bacteria are called pioneer colonizer, because they are hardy and successfully compete with other members of the oral flora for a place on the tooth surface. Slide 57 Primary colonizers D Caroline Mohamed57 Generally the primary colonizers are not in sufficiet number to be pathogenic. Overwhelming cocci, specially streptococci and short rods. Slide 58 Gram-positive facultatively anaerobic Streptococcus sanguis is most dominant first to appear followed later by S. Mutans. Actinomyces spp. are also found in 24h plaque. Gram-positive cocci and rods co-aggregate and multiply. 58D Caroline Mohamed Slide 59 This is followed by a stronger attachment by means of extracellular sticky polymers of glucose (glucans) which are synthesized by the bacteria from dietary sugars (principally sucrose). An enzyme on the cell surface of Streptococcus mutans, glycosyl transferase, is involved in initial attachment of the bacterial cells to the tooth surface and in the conversion of sucrose to dextran polymers (glucans) which form plaque. Bacteria adhere, multiply and increase in mass and thickness and form mini colonies in layers upon the pellicle (plaque formed by rod and cocci only). Time elapsed 24-48 hours D Caroline Mohamed59 Slide 60 Within a short time, the tooth surface adjacent to the gingiva is covered by intermeshed bacteria. New bacteria derived from saliva or surrounding mucous attach by a bonding interaction to bacteria already attached to the plaque. All this activity occurs within the first 2 days of plaque development and, for description purposes, is called phase I of plaque formation. Slide 61 In phase II of plaque development, the outer surface of the plaque is covered by gram-positive tall rods. There is a dramatic increase in plaque thickness 3 and 4 days compared to the first two days. Slide 62 Secondary colonizers 62D Caroline Mohamed Adhere to bacteria already in the plaque mass. Surface receptors on the Gram-positive facultative cocci and rods allow the subsequent adherence of Gram-negative organisms, which have a poor ability to directly adhere to the pellicle. Fn: Fusobacterium nucleatum. BI: Prevotella intermedia. Slide 63 Any area where the plaque has not been removed, either because of no home care or ineffective techniques, the bacteria mass continues to grow with a more complicated mixture of bacteria ( fusobacteria and filamentous). As the plaque thickens at the cervical area, the deeper layers incorporate more filaments and fusiforms, eventually turning gram negative. The coronal plaque is a more simple early arrangement of rods and cocci. Time elaped 4-7 days D Caroline Mohamed63 Slide 64 Micro-colonies D Caroline Mohamed64 Slide 65 Microcolonies coalescing D Caroline Mohamed65 Slide 66 In phase III, 4 to 7 days after initiation, plaque begins to migrate subgingivally, and bacteria and their products permeate and circulate in the pocket. The heterogeneity increases as plaque ages and matures. As a result of ecologic changes, more Gram-negative strictly anaerobic bacteria (fusobacteria, veillonellae) colonize secondarily and contribute to an increased pathogenicity of the biofilm. Slide 67 In phase IV, 7 to 11 days after initiation of plaque development, the diversity of the flora increases to comprise motile bacteria including spirochetes and vibrios as well as fusiforms and white blood cells appear. The plaque becomes more gram negative and anaerobic in the deeper layers, the gums become slightly inflamed. Time elapsed 7-14 days D Caroline Mohamed67 Slide 68 Vibrios and spiroquetes continue to multiply. The bacteria in the dental plaque become highly organized, filamentous, are perpendicular to the tooth surface, and pulsate in a palisade fashion. The signs of inflamed gums are obvious. Time elapsed 14-21 days D Caroline Mohamed68 Slide 69 The increasing thickness of the plaque limits the diffusion of oxigen to the entrapped original oxigen- tolerant populations, as a result the organisms that survive in the deeper parts of the plaque are either facultative or obligate anaerobes as fusobacteria and veillonese D Caroline Mohamed69 Slide 70 Filamentous bacteria D Caroline Mohamed70 Slide 71 corn-cob associations D Caroline Mohamed 71 Slide 72 This mature plaque is now so packed with diverse bacteria that an exogenous species would have great difficulty becoming established in the overcrowded habitat. With time, more bacteria migrate subgingivally, and the process continues more aggressively. Slide 73 Clean substratum Molecular adsorption (Phase 1) Single organisms (Phase 2) Multiplication (Phase 3) Sequential adsorption of organisms (Phase 4) 73D Caroline Mohamed Slide 74 Mature dental plaque D Caroline Mohamed74 Slide 75 Calculus Composition 1. inorganic content 70-90% of calcium phosphate, calcium carbonate, magnesium phosphate 2.organic content protein-polysaccharide, desquamated epithelial cells, leukocytes, microorganisms Slide 76 Calculus formation Calculus is dental plaque that has undergone miniralization Soft plaque.......precipitation of miniral salts between 1 st and 14 days Saliva is the source of miniralization Gingival Cervicular Fluid (GCF) Slide 77 Health Gingivitis Periodontitis Slide 78 Normal dental plaque: coccis, filaments, epithelial cell, little motility Gingivitis plaque: spirochetes and great motility Slide 79 The critical locus of activity is the subgingival space (periodontal pocket). The bacteria residing in the pocket and the host cells that defend it determine the clinical outcome. The diseased periodontal pocket harbors both attached subgingival plaque biofilms and nonattaching, motile subgingival microflora (spirochetes, vibrios, and straight rods with flagella). Slide 80 Orland et al (1954) demonstrated germ-free animals do not develop caries. In humans, when bacteria are allowed to accumulate in plaque on the tooth surfaces, enamel caries and gingivitis develop within 2 or 3 weeks. Slide 81 Periodontal Disease Slide 82 How do we know there is a problem?? DIAGNOSIS Individual complaine Clinical examinations Radiographic examination Slide 83 Individual complaine Bleeding gums Red gums Blood on my pilo Bad taste Bad smell (halitosis) Smokers....less bleeding Slide 84 Clinical examinations Plaque index Gingival index Pocket measurment Furcation Tooth mobility Slide 85 Slide 86 Plaque Scoring System for Quigley and Hein Score no plaque 0 flecks of stain at the gingival margin 1 definite line of plaque at the gingival margin 2 gingival third of surface 3 two thirds of surface 4 greater than two thirds of surface 5 Plaque Index total score = = SUM(scores for all faal and lingual surfaces) index = = (total score) / (number of surfaces examined) Slide 87 Slide 88 Gingival Index Le & Silness 1963 Score 0Score 2 Score 3Score 1 Slide 89 Gingival Index: GI 0 1 2 3 Slide 90 AppearanceBleedingInflammationPoints normalno bleedingnone0 slight change in color and mild edema with slight change in texture no bleedingmild1 redness, hypertrophy, edema and glazing bleeding on probing/pres sure moderate2 marked redness, hypertrophy, edema, ulceration spontaneous bleeding severe3 Gingival Index Le & Silness 1963 Slide 91 Teeth examined: (1) maxillary right first molar (2) maxillary right lateral incisor (3) maxillary left first bicuspid (4) mandibular left first molar (5) mandibular left lateral incisor (6) mandibular right first bicuspid Slide 92 Surfaces examined on each tooth: (1) buccal (2) lingual (3) mesial (4) distal Gingival Index Le & Silness 1963 Slide 93 Average Gingival IndexInterpretation 2.1 - 3.0severe inflammation 1.1 - 2.0moderate inflammation 0.1 - 1.0mild inflammation < 0.1no inflammation Gingival Index Le & Silness 1963 Slide 94 Sign the worse score Choose the worse score Sign the worse score