libiol fresh cells 2002 1/23 - doorirng.com cell.pdf · libiol fresh cells 2002 1/23 . 9mild, ......

LIBiol Fresh Cells 2002 1/23

Mild, selective enzymatic extraction

Cellular suspension

Natural microcapsules


Primary Cell wall

Secondary Cell wall

Cell membrane

Enzymatic extraction


Membrane

Central vacuoleVitaminsOrganic componentsMineral salts

Nucleus

Chloroplasts

Mitochondria


ClaimsVisualize nature’s smallest vesselVisualize nature’s smallest vesselPreservation of its structure Natural protection of the cell’s molecular load

BenefitsUse of all the cell componentsUse of all the cell componentsVery rich in mineral salts, vitamins and oligoelementsEnzymatic inhibition


Strawberry :Vitamin C, Ca++, Mg++Depurative, Stimulatetired skin

Kiwi :Very rich in Vitamin CAntioxidant & enzymatic inhibitorProtector for young skin

Mango :Pro vitamin A & Vitamin CFe++, Mg++To fight skin ageing

Carrot :ß-caroten, Potassium, SodiumHeal & murture the skinPrepare to sun exposure.

Pumpkin :ß-caroten, Mg++, Zn++Antioxidant & soothing propertiesIdeal for sun-burns or irritated skin


MINERAL SALTS DOSAGEFlame PhotometryAtomic Absorption Photometry

RADICAL SCAVENGER EFFECTSuperoxide Anion :

Reduction of the Nitro-blue-tetrazolium to Formazan blue.Radical DPPH :

Reduction ratio of the DPPH radicalHydroxyl Anion :

Reduction ratio of DMSO to formaldehyde

ENZYMATIC INHIBITIONHyaluronidase inhibitionTyrosinase inhibition


0,050,040,0090,020,03Zinc

68,0531,7962,344,2665,16Potassium

3,21,632,122,892,21Magnesium

0,440,270,390,330,28Iron

1,960,420,961,051,46Calcium

PumpkinMangoKiwiStrawberryCarrot

Results are expressed in mg for 100 g of productLIBiol Fresh Cells 2002 8/23

42,7%37,3%

31,4%

Inhi

bitio

n %

Fresh CellsPumpkin

Fresh CellsMango

Fresh Cells Kiwi

Tyrosinase inhibition

36%32,2% 31,3%

Inhi

bitio

n %

Fresh CellsStrawberry

Fresh CellsMango

Fresh CellsPumpkin

Hyaluronidase inhibition

In Vitro test : inhibition of tyrosinase is shown for all three references, ranking between 31% and 42%.

In Vitro test : Hyaluronidase is significantly inhibited for the references presented (31 to 36%), thus protecting the water-reservoir molecules in the skin – hyaluronic acid.

Product tested at 20%


R°

R-OO°

R-O-R : Molecular oxygen

O2°- : Superoxide anion

Peroxide

OH°- : Hydroxyl anion

Destruction of Destruction of biological moleculesbiological molecules(Fatty acids, Proteins, Sugar …)

The cellular suspensions have shown significant free-radical scavenging effects on hydroxyl, superoxide and DDPHradicals.

This inhibiting activity makes the Fresh Cells® an ideal ingredient for all anti-stress, anti-age, or protective cosmetic products.

Infernal c

ycle

UV, Pollution, Tobacco …


16,9% 16,4%

8,5%

Inhi

bitio

n %

Fresh Cells Carrot

Fresh Cells Kiwi

Fresh CellsPumpkin

Scavenger effect on DPPH

Product tested at 10%

Reduction ratio of the DPPH Radical, measured by spectrophotometry at 517nm

DPPH = 1,1 Diphenyl 2 –Picryl Hydrazyl


47,5%

12,40%

61,9%

32,40%

49,3%

17,90%

Inhi

bitio

n %

Fresh Cells Kiwi Fresh Cells Carrot Fresh Cells Pumpkin

Scavenger effect on superoxide anion

Product tested at 10%Product tested at 1%

Reduction ratio of the Nitro-Blue tetrazolium to formazan blue measured by spectrophotometry at 517nm.

Superoxide anion = O2°-


53,4%

22,3%

37,4%

Inhi

bitio

n %

Fresh Cells Kiwi Fresh Cells Carrot Fresh Cells Pumpkin

Scavenger effect on hydroxyl anion

Reduction ratio of the DMSO to Formaldehyde measured by spectrophotometry

Hydroxyl anion = OH°-LIBiol Fresh Cells 2002 13/23

Easy to formulate

Add at the end of the emulsification process

Stable in complex formulation1 year of stability


Strawberry Fresh CellStrawberry Fresh Cell®®in a in a body body milkmilk

Pumpkin Fresh CellPumpkin Fresh Cell®®in a in a body body milkmilk


Faculty of Faculty of ChatenayChatenay MalabryMalabry (Paris)(Paris)CofocalCofocal microscopy microscopy

CNRS Vegetal Physiology (Gif /Yvette)CNRS Vegetal Physiology (Gif /Yvette)Analysis of the cell’s structureAnalysis of the cell’s structure



Davines (Italy)

Bio O InvisibleINVISIBLE IMPACT, ANTI-FREE RADICAL ACTION, VOLUME, COMPLETE HYDRATION, RE-MINERALIZING EFFECT. FOR

FINE AND DELICATE HAIR. WITH PUMPKIN FRESH CELLS AND WHEAT

PROTEINS

Pacific (Korea)

Functional cosmeticPUMPKIN FRESH CELLS, STRAWBERRY FRESH CELLS,

CARROT FRESH CELLS, MANGO FRESH CELLS

Darphin (France)

Hydraskin RangeMANGO FRESH CELLS

KIWI FRESH CELLS

Quimibel (Spain)MANGO FRESH CELLS

KIWI FRESH CELLS

Brume pour le Corps JB 1315/D Body Lotion JB1315/D

Ingredients INCI DENOMINATION % W/W Function KIWI ORIGINAL EXTRACT (1)

ACTINIDIA CHINENSIS (KIWI) FRUIT WATER 97,2 Vegetal Water

KIWI FRESH CELLS (2) AQUA (AND) ACTINIDIA CHINENSIS (KIWI) FRUIT EXTRACT (AND) XANTHAN GUM

2 Active Ingredient

GLYDANT PLUS LIQUID (3) DMDM HYDANTOIN (AND) IODOPROPYNYL BUTYLCARBAMATE

0,3 Preservative

YELLOW E102 (sol at 0,5%) (4)

CI 19140 1 Coloring agent

FDC red 4 (sol at 0.1%) (4)

CI 14700 0,06 Coloring agent

GREEN E 102 / E 131 (sol at 0,1%) (4)

CI 19140/CI / CI 42051 0,1 Coloring agent

PEMULEN TR2 (5) ACRYLATES/C10-30 ALKYL ACRYLATE CROSSPOLYMER

0,05 Gelifying agent

SODIUM HYDROXIDE (sol at 10%)

SODIUM HYDROXIDE 0,06 Neutralizing agent

100 Appearance : Green lotion

Aspect : Lotion verte

Manufacturing Process : Mode opératoire : Spread the pemulen in the kiwi original extract. Add the coloring agents, meutralize. Then, under a soft stirring, add the glydant and the kiwi fresh cells.

Disperser le pemulen dans l’extrait originel de kiwi. Ajouter les colorants, neutraliser puis ajouter sous agitation lente le glydant et les cellules fraîches.

(1) – Gattefossé / (2) - Libiol / (3) - Lonza / (4) – LCW / (5) – Goodrich


Lait au Potiron MM7260 Pumpkin Milk MM7260

Ingredients INCI DENOMINATION %

W/W Function

Phase I PLUROL STEARIQUE (1) POLYGLYCERYL-6 DISTEARATE 5 PEG-Free O/W

Emulsifier SOFTCUTOL O (1) ETHOXYDIGLYCOL OLEATE 5 Emollient LABRAFAC CC (1) CAPRYLIC/CAPRIC TRIGLYCERIDE 5 Emollient DOW CORNING 200 (100 CS) (4)

DIMETHICONE 4 Feeling agent

PHENONIP (3) PHENOXYETHANOL (AND) METHYLPARABEN (AND) BUTYLPARABEN (AND) ETHYLPARABEN (AND) PROPYLPARABEN

0,5 Preservative

Phase II DEMINERALIZED WATER WATER 62,7 CARBOPOL ETD 2050 (6) CARBOMER 0,15 Gelifying agent RHODICARE S (2 % Sol.)(5) XANTHAN GUM 10 Gelifying agent Phase III SODIUM HYDROXIDE (10% Sol.) Phase IV

SODIUM HYDROXIDE 0.3 Neutralizing agent

DOW CORNING 1401 (4) CYCLOMETHICONE (AND) DIMETHICONOL

2 Feeling agent

Phase V SIGN’ESSENCE (1) 0,2 Perfume GLYDANT PLUS LIQUID (7) DMDM HYDANTOIN(AND)

IODOPROPYNYL BUTYLCARBAMATE 0,15 Preservative

PUMPKIN FRESH CELLS (2) AQUA (AND) CUCURBITA PEPO (PUMPKIN) FRUIT EXTRACT (AND) XANTHAN GUM

5 Active Ingredient

100 LIBiol Fresh Cells 2002 21/23

Masque Capillaire MM7261 Capillary mask MM7261


W/W Function

Phase I APIFIL (1) PEG-8 BEESWAX 2 O/W Emulsifier EMULCIRE 61 (1) CETYL ALCOHOL (AND) CETETH-20

(AND) STEARETH-20 1 O/W Emulsifier

STEARYL ALCOHOL LABRAFAC CC (1) SOFTCUTOL B (1) SHEA BUTTER PHENONIP (3)

STEARYL ALCOHOL CAPRYLIC/CAPRIC TRIGLYCERIDE ETHOXYDIGLYCOL BEHENATE SHEA BUTTER PHENOXYETHANOL (AND) METHYLPARABEN (AND) BUTYLPARABEN (AND) ETHYLPARABEN (AND) PROPYLPARABEN

1,5 4 2 2 0,5

Thickener Emollient Texture agent Emollient Preservative

Phase II DEMINERALIZED WATER WATER 60,3 AVICEL RC (4) RHODICARE S (2% Sol.) (5)

MICROCRYSTALLINE CELLULOSE (AND) CELLULOSE GUM XANTHAN GUM

1,5 20

Gelifying agent Gelifying agent

Phase III SIGN’ESSENCE (1) 0.2 Perfume MANGO FRESH CELLS (2) AQUA (AND) MANGIFERA INDICA

(MANGO) FRUIT EXTRACT (AND) XANTHAN GUM

5 Active Ingredient

100.00


Lait corporel à la fraise JB 1306/A Strawberry body Milk JB 1306/A


W/W Function

Phase I TEFOSE 2561 (1) PEG-6 STEARATE (AND) CETETH-20

(AND) GLYCERYL STEARATE (AND) STEARETH-20

5 Emulsifier

MOD (1) OCTYL DODECYL MYRISTATE 8 Emollient VEGETOL CALENDULA WL1072 (1)

CALENDULA OFFICINALIS FLOWER EXTRACT

2 Active ingredient

CETYL ALCHOL CETYL ALCHOL 1 Texture agent DOW CORNING 200 (100CS) (2)

DIMETHICONE 3 Feeling agent

PHENONIP (3) PHENOXYETHANOL (AND) METHYLPARABEN (AND) BUTYLPARABEN (AND) ETHYLPARABEN (AND) PROPYLPARABEN

0,5 Preservative

Phase II DEMINERALIZED WATER WATER 75 CARBOPOL 981 (4) CARBOMER 0,1 Gelifying agent Phase III SODIUM HYDROXIDE (sol at 10%)

SODIUM HYDROXIDE 0,2 Neutralizing agent

Phase IV STRAWBERRY FRESH CELLS (5)

AQUA (AND) FRAGARIA VESCA (STRAWBERRY) FRUIT EXTRACT (AND) ASCORBIC ACID (AND) XANTHAN GUM

5 Active Ingredient

100 Appearance : Pink milk

Aspect : Lait Rosâtre

Manufacturing Process : Mode opératoire : Spread the Carbopol in water. Disperser le carbopol dans l’eau.

Under stirring, at 75°C, add I to II, then neutralize.

Sous agitation, à 75°C, ajouter les composants de I à II, puis neutraliser.

Cool and at 35°C, under slow stirring add IV. Laisser refroidir et vers 35°C, sous agitation modérée ajouter IV.

(1) - Gattefossé / (2) – Dow Corning / (3) - Nipa / (4) – Goodrich / (5) – LIBiol


Voile de Carotte JB 1320/A Complexion Enhancer JB 1320/A


W/W Function

Phase I DOW CORNING 345 (1) CYCLOMETHICONE 5 Feeling agent LABRAFIL ISOSTEARIQUE (2)

TRIISOSTEARIN PEG-6 ESTERS 1,5 Disperser

CAROTENE HUILEUX 1000 (2)

APRICOT KERNEL OIL PEG-6 ESTERS (AND) BETA-CAROTENE

1 Free radical scavenger

PARSOL MCX (3) OCTYL METHOXYCINNAMATE 1 UV filter PHENONIP (4) PHENOXYETHANOL (AND)

METHYLPARABEN (AND) BUTYLPARABEN (AND) ETHYLPARABEN (AND) PROPYLPARABEN

0,5 Preservative

Phase II DEMINERALIZED WATER WATER 75,4 PROPYLENE GLYCOL PROPYLENE GLYCOL 5 Moisturizer GLYCERIN GLYCERIN 3 Moisturizer ETD 2020 (5) ACRYLATES/C10-30 ALKYL ACRYLATE

CROSSPOLYMER 0,5 Gelifying agent

Phase III SODIUM HYDROXIDE (Sol at 10%)

SODIUM HYDROXIDE 1 Neutralizing agent

Phase IV BRUN COVASCOP W8772 (6) CI 77491/CI 77492/ CI 77499 0,4 Colouring agent FDC RED 4 (sol at 1%) (6) CI 14700 0,25 Colouring agent TIMIRON KARAT GOLD MP24 (7)

MICA (AND) TITANIUM DIOXIDE (AND)IRON OXIDES

0,25 Colouring agent

PERFUME SIGN ESSENCE (2)

0,2 Perfume

CARROT FRESH CELLS (8) AQUA (AND) CAROTA (CARROT) FRUIT EXTRACT (AND) XANTHAN GUM

5 Active Ingredient

100 LIBiol Fresh Cells 2002 24/23

libiol fresh cells 2002 1/23 - doorirng.com cell.pdf · libiol fresh cells 2002 1/23 . 9mild, ......

Documents