l’exemple de la listériose - infectiologie
TRANSCRIPT
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Pathophysiologie des infections fœto-placentaires: l’exemple de la listériose
CEMI 18 – 15 mars 2013 Institut Pasteur
Olivier Disson, PhD Biology of Infection Unit
Institut Pasteur, Inserm U1117
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Intestinal barrier asymptomatic
mild gastroenteritis
Blood-brain barrier meningitis, encephalitis
Placental barrier fetal and neonatal infections
Successive steps of human listeriosis
Lymph node
Contaminated food Liver
Spleen
Blood
Blood
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Pregnancy-related listeriosis in France
• Correlation of decrease in pregnancy associated cases with dietary recommendations
• In 2012: 38 pregnancy associated cases / lethality around 30% (338 total listeriosis cases)
• A prospective study on risk factors (MonaLisa) is ongoing: 100 pregnancy-associated cases included
Contrôle des produits fromagers
Contrôle des produits carnés
Recommandations auprès des femmes enceintes
Adapted from Goulet et al, EID 2001 Goulet et al, BEH 2012
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Listeria monocytogenes
InlA
Human
E-cad
Listeria crossing of the host barriers
• InlA induces internalization in cultured epithelial cells
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Listeria monocytogenes
InlB
Human
Met
• InlA induces internalization in cultured epithelial cells
• InlB induces internalization in cultured epithelial cells
Listeria crossing of the host barriers
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Listeria monocytogenes
InlA InlB
E-cad
Guinea pig Rabbit
Mouse Rat
Human
E-cad Met Met Met E-cad
Listeria crossing of the host barriers
• InlA and InlB induce internalization in cultured epithelial cells
• InlA-Ecad and InlB-Met interactions are species-specific
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Listeria monocytogenes
InlA InlB
E-cad E-cad Met Met Met E-cad
hEcad
Guinea pig Rabbit
Mouse Rat
Human
Lecuit et al, Science 2001
Listeria crossing of the intestinal barrier
• InlA and InlB induce internalization in cultured epithelial cells
• InlA-Ecad and InlB-Met interactions are species-specific
• Critical role for InlA in Listeria crossing of the intestinal barrier, no role for InlB
- Demonstrated in guinea pigs and in humanized mice expressing human E-cadherin only at the intestine level
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Unexpected observation
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LRRs IR
p120ctn
Actin
E-cadherin
entry
L. monocytogenes
full-length InlA
Some L. monocytogenes isolates express a truncated InlA
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LRRs IR
p120ctn
Actin
E-cadherin
truncated InlA
entry
L. monocytogenes
Some L. monocytogenes isolates express a truncated InlA
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1- Questions the role of InlA in humans 2- Offers the opportunity to evaluate its role “epidemiologically”
Some L. monocytogenes isolates express a truncated InlA
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Clinical strains (N=300)
96% FL InlA
Food strains (N=150)
65% FL InlA
P<1x10-7
• A role for InlA in crossing the intestinal barrier in humans
• Demonstrates the relevance of the iFABP-hEcad Tg model
Bacteremia 93.2% FL InlA
Epidemiological approach
- WB with anti-InlA Mabs Full length (FL) or truncated InlA? - Compare InlA expression profile of strains of clinical or food origin
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Bacteremia 93.2% FL InlA
CNS infection 98.2% FL Internalin
Fetoplacental infection 100% FL InlA
• In favor of a role for InlA in L. monocytogenes placental tropism
P<1x10-7 P=4x10-2
Food strains (N=150)
65% FL InlA
Epidemiological approach
- WB with anti-InlA Mabs Full length (FL) or truncated InlA? - Compare InlA expression profile of strains of clinical or food origin
Jacquet et al, JID 2004
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The human maternofetal barrier
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Histopathological study of placenta from women with listeriosis
Free bacteria Adhesion
Invasion Crossing Multiplication
Invasion Crossing Multiplication
Intravillous abscesses
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Amnion infection from the amniotic fluid
Amniotic fluid
Uterine wall
Suggests that L. monocytogenes crosses the materno-fetal barrier at the villous level
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If internalin plays a role in targeting the placental barrier, E-cadherin is expected to be expressed at the villous level
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accessible hEcad
total hEcad
E-cadherin is expressed at the interface between the maternal blood and the fetal tissue
i.e. the syncytiotrophoblast apical membrane
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fetal side maternal side villous explant
Use of human placental explants
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Histology
3h: Adhesion to and invasion of the syncytiotrophoblast
Gram staining
IF staining
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Histology
24h: Crossing of the placental barrier Development of villous abscesses
similar to actual placental villous
abscesses from women
with listeriosis
Lecuit et al. PNAS 2004
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Internalin-dependent infection of the fetal side of the amnion
hEcad labeling of the amnion
fetal side
maternal side
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Role of internalin at the human maternofetal interface
Secondary dissemination to the amnion
Targeting and crossing of the placental barrier
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An animal model for human fetoplacental listeriosis ?
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Double species specificity of InlA and InlB pathways
Ex vivo: InlA+InlB Epidemiological data suggest a role for InlA
In apparent contradiction with human ex vivo and epidemiological data
NO InlA-dependent placental invasion Bakardjiev, IAI 2004
NO InlB-dependent placental invasion Le Monnier, IAI 2004
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Hypothesis :
Both InlA AND InlB pathways have to be functional to mediate L. monocytogenes invasion of the human placenta
Need for an animal model permissive to BOTH InlA and InlB Existing Tg mice express hEcad only at the intestinal level…
Use of gerbils, permissive for both InlA and InlB
Listeria crossing of the placental barrier
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Real time in vivo imaging of maternofetal listeriosis
DinlAB
Day 1
Day 4
Day 5
WT DAB DAB
Intravenous infection. Bioluminescent bacteria.
WT
WT
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Day 1
Day 4
Day 5
DAB DAB
WT WT
WT
DinlAB
Intravenous infection. Bioluminescent bacteria.
Real time in vivo imaging of maternofetal listeriosis
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Day 1
Day 4
Day 5
InlA and/or InlB are required for placenta and fetus infection, in contrast to what observed in mice and guinea pig
WT WT
WT
DinlAB
DinlAB
Intravenous infection. Bioluminescent bacteria.
Real time in vivo imaging of maternofetal listeriosis
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Listeria crossing of the placental barrier
Placenta 72h post infection
Epithelium Endothelium Listeria Nuclei
• L. monocytogenes can cross the placental barrier in intravenously infected gerbils
Placenta 24h post infection
Epithelium Endothelium Listeria Nuclei
Epithelium Endothelium Listeria
Placenta 42h post infection
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Placenta
One point = one organ * P<0,05 ** P<0,01
*** P<0,001
L. monocytogenes invasion of gerbil placenta and fetuses C
I = R
atio
EG
D w
tr / S
trai
ns
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Placenta
Rat
io o
f C
mrr a
nd
C
ms
bac
teri
a
One point = one organ * P<0,05 ** P<0,01
*** P<0,001
Fetus
InlA and InlB allow the crossing of the placental barrier
CI =
Rat
io E
GD
wtr /
Str
ain
s L. monocytogenes invasion of gerbil placenta and fetuses
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InlB non functional NO role for InlA in placental invasion
in vivo
InlA non functional NO role for InlB in placental invasion
in vivo
InlA AND InlB functional Role for BOTH InlA and InlB in
placental invasion in vivo
and gerbil
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and gerbil
Knock-in mice expressing a humanized E16P mEcad
Humanized E16P mouse
E16P mEcad
Genetic approach
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- Knock-in strategy homologous recombination in ES cells
- E16P mEcad expression pattern similar to mEcad physiological expression
notably at the intestinal, placental, and blood-brain barrier levels
- Functional receptors for InlA and InlB assess the role of InlA and InlB in a fully permissive animal model
KI mice expressing a humanized E16P mEcad
Placental barrier
Intestinal barrier
Blood-brain barrier
F1 chimera
KI mice expressing a humanized E16P mEcad
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Primary enterocytes from humanized E16P mice are permissive to InlA
Listeria innocua L. innocua (InlA)
WT mice
E16P+/+ mice
Ecad Listeria Nuclei
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Quantitative assessment of the roles of InlA and InlB at the fetoplacental level
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Conclusions & Perspectives
- Fetoplacental invasion is controlled by two species specific interactions, which are indeed interdependent
InlA - E-cadherin InlB - Met
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- Fetoplacental invasion is controlled by two species specific interactions, which are indeed interdependent
InlA - E-cadherin InlB - Met
- Specific microbial targeting to the placenta, in addition to “pregnancy-associated immunosuppression”
Conclusions & Perspectives
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- Fetoplacental invasion is controlled by two species specific interactions, which are indeed interdependent
InlA - E-cadherin InlB - Met
- Specific microbial targeting to the placenta, in addition to “pregnancy-associated immunosuppression”
- Both pathways have to be functional for fetoplacental invasion, in contrast to what observed for intestinal invasion
Conclusions & Perspectives
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Intestinal barrier
Placental barrier
Intestinal barrier
Placental barrier
InlA + InlB -
InlA + InlB +
• Which mechanism(s) explain the difference between intestinal and placental barriers ?
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Collaborations
Institut Pasteur Pascale Cossart Charles Babinet Francina Langa-Vives
Biology of Infection Unit, Institut Pasteur, Inserm U1117
Marc Lecuit Solène Grayo Eugénie Huillet Olivier Disson Laetitia Travier Camille Blériot Cindy Fèvre Yu-Huan Tsai Grégoire Gessain Thérèse Couderc Nicolas Gangneux Delphine Judith WHO & French National Reference Centre on Listeria Alexandre Leclercq Caroline Charlier-Woerther Viviane Chenal-Francisque
Funding
Acknowledgments