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>FieldPhenotyping

>Highresolutionfluorescence–

Fv/Fm for PSII activity

>Hyperspectralimaging

NEWlife science research 

high-throughput screening 

plant functional genomics 

ecotoxicology

high-content screening 

cell analysis 

colony counting

drug discovery

quality control

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life science research 

high-throughput screening 

plant functional genomics 

ecotoxicology

high-content screening 

cell analysis 

colony counting

drug discovery

quality control

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Dipl. Biol.Dirk VandenhirtzCEO

join

 the 

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The challenges LemnaTec’s ambition is to reproduc-

ibly quantify human vision on organ-

isms. Therefore the LemnaTec scan­

alyzer platform generates digital images

in a wide range of wavelengths – far beyond

human vision. The LemnaTec software auto-

matically analyses these images, resulting in a

considerable number of quantitative visual parameters.

LemnaTec develops imaging and automated plant man-

agement as well as plant treatment systems, image

analysis and data handling modules and also

strategies to meet the needs of today’s re-

search and industry. We enable customers to

design and develop products suitable

for future challenges.

The targetsLemnaTec’s team of

scientists develops

hard- and software

solutions for highthroughput

and high-content screening of plants, seedlings, insects

and other organisms and for the automated evaluation of

bio tests in ecotoxicology. Digital images are primarily

taken by the scanalyzer systems PL, HTS, Field and 3D,

all set up in a modular design. Using advanced LemnaTec

image processing algorithms, every visible sample parameter

(e. g. colour, shape, size, architecture) is subsequently measured

and correlated with experimental records (e. g. genetic data). Our

aim: to visualise and analyse the biology beyond human vision.

Page 3

143 / -308 The tools – hardwarescanalyzer3D

manual loaded imaging units with top and side imaging capabilities for 3D plant phenotyping

scanalyzer3D HT

automatic imaging and transport of plants in greenhouses and growth chambers, combined with watering and spray-ing systems, providing standardised growth conditions and non-destructive daily measurements

scanalyzerfield

a wide range of choices for the quantitative, non- destructive analysis of different crops or model plants in field conditions

scanalyzerHTS

high-throughput, high-content screening for agricultural, phenotypical and pharmaceutical purposes

scanalyzerPL

the basic system for all quantitative biological imaging –from ecotoxicology and small plant analysis to colony counting and seed analysis

The tools – softwareLemnaLauncheruniversal interface for machine/analysis/database management of all scanalyzer systems

LemnaControlcontrol center for imaging and plant/data handling, robots and greenhouse or growth chamber logistics

LemnaGridhighly flexible image analysis development kit for advanced imaging tasks

LemnaMinerdata mining and data correlation tool to generate biologically relevant information

LemnaBasedata handling medium offering consistent and adequate data storage of up to 100 terabyte

LemnaSharesupervised exchange platform for LemnaTec image analysis grids

-151 / -592

Page 3

scan

alyze

r3D

Accelerating plant phenomicsLemnaTec scanalyzer3D systems open new prospects for the quantitative, non-destructive analysis

of different crops or model plants under high-throughput conditions. Each plant is imaged sequen-

tially in multiple scanalyzer3D camera units, employing different wavelengths that reach far beyond

human vision. The result is an unprecedented number of reproducible and significant data points

on any aspect of plant development.

Data will be collected continuously and recorded in one snapshot per run through the

scanalyzer3D, thus routinely producing 15 or more images and several hundred data points per

plant and run. Plants are imaged in multiple runs throughout their entire life cycle. All data is

available in the database within minutes of imaging – for unparalleled monitoring of dynamic

plant development. Comprehensive quantitative representation of plants in datasets generates

special data for physiological and genetic plant modeling to identify and quantify the concealed

parameters that really control plant development. Such deep insights into plant biology accelerate

phenomics and enable plant breeding to deal with the challenges of the 21st century by measuring

dynamic data in relatively early stages of plant growth.

1. Scanning – infrared light With infrared light, high-throughput 3-D imaging systems

provide a unique chance to quantify temperature differences

(e. g. within leaves and between plants).

2. Scanning – visible light High-resolution colour images taken from the top and several sides under

reproducible illumination conditions provide the best basis for comprehen-

sive phenotyping.

4. Scanning – near infrared light 

Visualisation of water distribution and dynamics (1450-1550 nm) as well as

further NIR-absorbing ingredients.

5. Scanning – fluorescent lightUsing blue light large field excitation (< 500 nm) in back/top light condi-

tions visualises any related fluorescence between 520 and 750 nm (GFP,

phenolics, etc).

3. Scanning – near infrared light for rootsHumidity visualisation in root columns by near infrared light provides added

information on spatial root performances of plants under high-throughput

conditions.

1.2.

3.4.

5.

Page 5

Fv/F

mfo

rPSI

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ivity

hype

rspe

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g

LemnaTec is pleased to announce the release of its new high-

throughput imaging technology for physiologically related mea-

surements of photosynthesis. The measurement principle is based

on imaging the Kautsky induction or fast chlorophyll fluorescence

induction curve of photosynthesis using one high intensity light

pulse for 1 – 3 s.

Unlike some other technologies, LemnaTec is able to provide a

technology solution that combines fast and efficient high-through-

put screening of photosynthetic activity with flexibility to offer:

n Whole plants of different sizes imaged remotely

and yielding low noise at high resolution images

n Dark and light adapted plant measurements

protocols including adaption tunnels

n Measurements tolerant to leaf angle for side and

top imaging in high throughput applications

n LED flash ranging up to 5000 µmol/m²s to measure

even under high light adaptation

darkadapted:no significant effect

lightadapted:significant effect

control droughtstressed

Photosynthesis Fluorescent Imaging

Multi-Disciplinary Research Applications

n State of the art camera

n Retrofit to all existing scanalyzer3D and scanalyzerHTS possible

n Optimised for visible and near infrared applications

Hyperspec ImagingHyperspectral imaging is a technology widely used for remote im-

aging, in an effort to extract a maximum of information out of

images made under strongly varying imaging conditions, caused by

the variability of the sunlight and changing atmospheric conditions.

Hyperspec=High Spectral and Spatial Resolution

n 1 spatial pixel measured at up to 930 wavelengths

n Each Pixel contains a continous spectrum that is

used to identify the condition of the plant or fruit

n E.g.: Optimised spectral detail in Chlorophyll red

band for more accurate classifications

one pixel

=Wavenumber [cm-1]

Imag

e [Y

]

Image [X]

Page 7

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5

6

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7

8

10

11

12

13

14

15

9

10

11

12

13

14

15

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Spra

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5830

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5992

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1702

2x24

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= 12

48

palle

ts

scan

alyze

r3D H

T

LemnaTec scanalyzer3D HT systems open new prospects for the quantitative, non-

destructive analysis of different crops or model plants under high-throughput

conditions. Each plant is transported automatically to multiple scanalyzer3D

imaging units using high quality conveyor belt systems. The result is an unprece-

dented number of reproducible and significant data points on any aspect of plant

development without any human interaction required. LemnaTec’s high den-

sity plant carrier system also provides options to equip growth rooms with

scanalyzer3D HT systems to achieve full control of environmental conditions and

significantly reduced experimental variability.

Watering and weighing systemsThe LemnaTec plant conveyor system includes the provision of high-precision

and controlled watering – it is geared to each individual plant’s requirements

(watering based on pot weight which is related to soil moisture) allowing

researchers to impose many kinds of saline stress or drought stress regime.

Spraying cabinetsLemnaTec’s comprehensive approach to plant management with its conveyor

system includes further options including spray cabinets for pest management

with commercial pesticides where low-drift nozzles and an efficent exhaust sys-

tem minimise spreading of the active ingredients within the whole greenhouse

or growth chamber. Special spray tables for manual spraying allows even plant

specific treatment with pesticides or test solutions avoiding cross contamination

to other plants (e. g. non-treatment controls).

Enhancing the statistical power  of greenhouse and  

growth room  trials

Page 9

scan

alyze

rfield

n Small plots for prebreeding

n Development of fine-tuned crop models

n Highly dynamic plant stress testing

such as drought stress

n High resolution phenotyping as

reference for remote sensing

n Growth assessment

n Crop physiology

n Climate change

n Water use efficiency, soil water

content, waterlogging

n QTL analysis

n Width up to 10 m

n Length up to 40 m

n Height up to 6 m

n Other custom sizes are also available

n Durable construction

n Minimised shading effects

n Reproducible 3D positioning for

cameras, scanners and sensors.

Multi-Disciplinary Research Applications

Designed for non-destructive digital phenotyping in rain-out shelters, lysimeter sys-

tems and small plot phenotyping, the scanalyzerfield offers superb precision, accuracy,

resolution and predictive capabilities for research in:

Quality Engineering for field conditions

Hence, the scanalyzerfield has the following features allowing digital phenotyping

of a multitude of crops, small trees and plants in specialized containers:

Field phenotyping is the classical strategy to assess qualitatively and quantitatively

agronomical important traits for field-grown plants. Many assessment methods are

based on direct visual assessment or the manual use of portable measurement units.

Considering the increasing amount of non-destructive, image-based measurement

systems that have become available in the last few years, the scanalyzerfield provides

the new option to acquire a wide range of image based data both with spatial and

temporal resolution quantifying how large numbers of plants react individually on

the highly dynamic environmental conditions in the field.

    Precision Robotics –      Imaging sensors include:

n Visible light

n Near infrared (water imaging)

n Infrared (heat imaging)

n Multispectral imaging

n Fluorescence

n Laser height profiling

scanalyzerfield – Phenotype  your crops under field conditions

Page 11

scan

alyze

rHTS

Small plant phenotypingThe scanalyzerHTS is the best option for the analysis of small plants in

multiwell plates or trays in specific growth chambers. The closed cabinet

– containing backlight/direct or diffuse top light illumination as well as

fluorescent imaging with blue light excitation – guarantees reproducible

imaging conditions for all camera types provided by LemnaTec (VIS, NIR,

IR, and Fluorescence).

Positioning can be easily exchanged to accommodate varying plant con-

tainers and illumination types. Zoom lens systems allow imaging of fully

grown Arabidopsis rosettes or complete multiwell plates/6 wells of a

96-well plate. Precise camera movement and automated tray identifica-

tion by barcode enable the reliable identification of individual plants.

3-D laser scans provide further information about plant height pro-

files. While the scanalyzerHTS is designed as a measurement instrument,

customised solutions may be placed in growth chambers.

Page 13

scanalyzerHTS – high-throughput screening in perfectionThe LemnaTec scanalyzerHTS is the preferred platform for high-

throughput screening of leaf disk assays, fungi, insects or

small aquatic/terrestrial organisms such as mosquito larvae or

nematodes of fish embryos. All four LemnaTec camera systems

(Visible, Fluorescence, Infrared, Near Infrared) and height scan-

ning can be applied to achieve even more detailed, single-well

scanning for high-resolution growth analysis. Depending on the

number and type of samples the cameras move over an array

of up to 84 multiwell plates, achieving an assessment of up to

8064 different samples in one run. Additionally available robots

store plates or place them in different functional locations.

Analysing movement of small organismsscanalyzerHTS provides unique opportunities to simultaneously observe large numbers of small moving organ-

isms. The observation time for each well ranges from seconds to minutes, depending on organism velocity.

Movement patterns and the localisation/intensity of the movement can be quantified. Repeated measurement

runs allow quantification of movement development over time, which is particularly useful for fast developing

organisms (fish embryos). In all cases static image

information is combined with movement in-

formation to assign movement patterns to

single organisms.

scan

alyze

rHTS

High content & high throughput: screening for basic research, pharmaceutical trials and plant protection 

Page 15

Sensor Size Sensitivity

VIS Camera 2448 x 2050 pixels 380 – 740 nm

Vis (High Res.) Camera 6576 x 3484 pixels 380 – 740 nm

Flu­GFP Camera 1392 x 1040 pixels 510 – 740 nm

Flu­PSII Camera 1388 x 964 pixels 680 – 900 nm

NIR Camera 640 x 512 pixels 900 – 1700 nm

IR Camera 320 x 240 pixels 8 – 14 µm

Hyperspec Camera 2560 x 2160 pixels VIS, NIR

LemnaTec scanning – The optimum in automated biological imaging

scan

alyze

rPL

Low throughput, but  a great deal of options For all low-throughput applications with manual

sample exchange, the scanalyzerPL combines

compact design and reproducible imaging tech-

niques with the comprehensive control of the

Lemna Launcher software.

Test containers may range from beakers for

ecotoxicity to trays with plants, smaller plants in

pots or even highly reflecting fruits. Depending

on the test purpose, different camera types with

specific illumination systems can be mounted.

The benchtop platform is available with different

back and top light modules.

cam

era

spec

ifica

tions

Spectral sensitivity NIR-Camera

Camera Specifications

Page 17

software architecturelinking data acquisition with analysis, creating and optimising data flows 

LemnaLauncherLemnaControlThis comprehensive and intuitive interface is the main framework granting access

to all LemnaTec software products across the entire product range. All research

images, analysis results and meta data are accessible within LemnaLauncher

and are specific for the individuals product configuration and image analysis

settings. This provides complete data management and ultimately a measure

of quality assurance. Integration of all software modules provides a complete

digital phenotyping workflow – from data acquisition to image analysis to sta-

tistical result presentation in LemnaMiner. Additionally, continuous processes

accelerates result generation and analysis without human input, guaranteeing

maximum data integrity.

Monitoring the fully integrated automati­

sation of all LemnaTec scanalyzer systems

LemnaControl is the tool to regulate and moni-

tor all appropriate system components includ-

ing cameras, conveyors, robotics, illumination

and thus enable trained users, with or without

any technical background to use the system.

LemnaControl enables the user to initiate an

experimental run on the LemnaTec scanalyzer 

rapidly and easily. An experimental run produc-

es all data including images, watering, weight

information and stores them into the database.

LemnaLauncher LemnaLauncherLemnaControl LemnaBase LemnaVision

LemnaBase LemnaVisionConvenient data storage

and backup solutions

The core database structure based on Oracle or

Linux PostgreSQL technology can handle data-

sets of up to 100 TB. The open interface allows

direct integration into any LIMS environment.

Combining a minimum of access time and a

maximum of transparency, LemnaBase ensures

the quality and lasting value of all acquired

data and related parameters.

Supervise the system

from anywhere

To supervise the LemnaTec scanalyzer Platforms

remotely from outside the greenhouse or the

growth room, dedicated power over Ethernet

camera solutions can be used in combination

with LemnaVision. LemnaVision enables the

user to watch and record multiple live streams

at the same time on different PCs, iPhones/

iPads or on dedicated displays in public areas.

Page 19

LemnaGrid

LemnaShare

LemnaMiner

LemnaMiner

LemnaShareExchange knowledge with

users worldwide

LemnaGrid image analysis grids can be

supplied online, as well as shared and

exchanged, by using the LemnaGrid ex-

change interface. The incoming grids,

transferred to LemnaShare by the users,

are validated and classified by LemnaTec

to be used by all customers as a basis

for their own work or other ready-to-use

solutions. Thus the risk of double devel-

opments is minimised and the ongoing

process of extracting increasingly relevant

biological data from images can be accel-

erated.

softw

are 

LemnaGrid LemnaShare LemnaMinerLemnaMiner

LemnaGrid LemnaMinerImage Processing without

programming knowledge

LemnaGrid is the image analysis tool

to address the specific needs in phe-

notyping with a maximum amount of

flexibility. The image analysis is graph-

ically represented as a process chain

and can be subsequently saved as a

LemnaTec image analysis grid. Once

the chain is complete it can be used to

automatically analyze all images of an

experiment in the background.

Data extraction with a

user friendly interface

The LemnaMiner software module

enables the researcher to display,

filter and export resulting data from

LemnaGrid to correlate it with other

experimental data and transform it

into biological relavant information. To

ensure data integrity within the data-

base, LemnaMiner saves a local copy

of the queried results in a SQLite File

on the workstation.

analysis network and accelerate image processing

Page 21

applicationsget phenotyping up to speed

Growth Plot

Correlation Study

Plant phenotyping & functional genomicsNon-destructive, multispectral plant imaging and comprehensive control of water

evaporation/supply allow optimal assessment of GxE impact on phenotypes under

stress or natural field conditions.

Breeding of new traitsQuantitative phenotype assessment of seed collections/crossing programmes guaran-

tees fast data access, even with complex traits such as drought tolerance.

High-content & high-throughput screeningMulti-parameter assessment of a wide range of organisms and assay types across thou-

sands of samples provides a reliable basis for agrochemical/pharmaceutical screening.

Life science research & drug discoveryAssessment of small organisms (nematodes, fish eggs, parasites) and cell cultures – new

prospects for the screening of active ingredients and pharmacological key structures.

Correlation  to manual  measurement

The Lemnatec scanalyzer systems provide

powerful predictive capabilities that are

strongly correlated to manual measure-

ments but enable research to do high

throughput screening for thousands of

assays.

When comparing traditional measure-

ments like fresh weight, size or number

of leaves to digital phenotyping param-

eters correlations of up to 95 % can be

established. Appropriate data analysis

and data modeling can help to establish

correlations between growth rates and

field yield of up to 75 % within the first

5 weeks.

Colony counting & cell analysisColony counting and automatic documentation is

getting increasingly important for food, beverage

and pharmaceutical products.

EcotoxicologyLemnaTec provides comprehensive imaging solu-

tions for standardised and research tests (duck-

weed, daphnids, nematodes), thus maximising

the quality of test results.

Quality controlAll scanalyzer hard- and software modules can be

customised to target most quality control solutions

in agricultural or pharmaceutical applications.

Application examples for the scanalyzer image analysis The highly flexible and easily customisable software framework covers a wide range of applications.

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LemnaTec GmbHPascalstr. 5952076 AachenGermany

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Web: www.lemnatec.comMail: info@lemnatec.comTel.: +49 (0) 24 08 / 93 83-0Fax: +49 (0) 24 08 / 93 83-100

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