leica macrofluo...macroscope with vertical beam path, motorized, apochromatic zoom system, incident...

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Leica MacroFluo™

Technical Information

3

Leica MacroFluo™ System, equipped with Leica Z16 APO A macroscope, Leica DFC camera, motorized zoom, transmitted light base TL RCI™, SmartTouch™ control panel, and Leica EL6000 fluorescent light source.

4

Designation Leica MacroFluo™ with 6.3:1 zoom Leica MacroFluo™ with 16 : 1 zoom

Type of microscopeMacroscope with vertical beam path, apochromatic zoom system, incident illuminator, fluorescence illumination coupled with the optical zoom system, and trinocular tube

Zoom system Apochromatic 6.3 : 1 zoom Leica Z6 APO, lead-free Apochromatic 16 : 1 zoom Leica Z16 APO, lead-free

Zoom factor 0.57 – 3.6× 0.57 – 9.2×

Engageable zoom positions 0.57 / 0.8 / 1 / 1.25 / 1.6 / 2 / 2.5 / 3.2 / 3.6 0.57 / 0.8 / 1 / 1.25 / 1.6 / 2 / 2.5 / 3.2 / 4 / 5 / 6.3 / 8 / 9.2

TubesBinocular phototube HC L2TU 4/5/7 with photo-TV output 100%:0% / 0%:100%, 20° viewing angleTrinocular tube BDT 25+V100/50/0 with photo-TV output 100%:0% / 50%:50% / 0%:100%, 30° viewing angle Tube BDTP 25 100/50/0 with fixed port, photo-TV output 100%:0% / 50%:50% / 0%:100%, 30° viewing angle

Built-in iris diaphragm For continuous depth of field control

Designation Leica MacroFluo™ with automated 6.3 : 1 zoom Leica MacroFluo™ with automated 16 : 1 zoom

Type of microscopeMacroscope with vertical beam path, motorized, apochromatic zoom system, incident illuminator, fluorescence illumina-tion coupled with the optical zoom system, and trinocular tube

Zoom system, motorized Apochromatic 6.3 : 1 zoom Leica Z6 APO A, lead-free Apochromatic 16 : 1 zoom Leica Z16 APO A, lead-free

Zoom factor 0.57 – 3.6× 0.57 – 9.2×

Motorized zoom Continuously motorized and approachable positions Continuously motorized and approachable positions

TubesBinocular phototube HC L2TU 4/5/7 with photo-TV output 100%:0% / 0%:100%, 20° viewing angleTrinocular tube BDT 25+V100/50/0 with photo-TV output 100%:0% / 50%:50% / 0%:100%, 30° viewing angle Tube BDTP 25 100/50/0 with fixed port, photo-TV output 100%:0% / 50%:50% / 0%:100%, 30° viewing angle

Motorized iris diaphragm For continuous depth of field control For continuous depth of field control

Motorized fine focus Integrated, 10 mm travel Integrated, 10 mm travel

Fluorescence illuminator

Type

Coaxial incident illuminator L4/23 and carrier for incident light fluorescence and incident light brightfield with:• Reflector disc for 5 filter blocks, 0-pixel technology = no pixel offset when changing• Aperture and field diaphragm can be centered• Switchable blue filter BG 38 and UV light stop• Light trap for suppression of ambient light

Fluorescence filter• Filter system (excitation filter, beam-splitting mirror, and barrier filter) on a filter block• Extensive filter block selection, filter combinations based on customer specifications

Light source

Leica EL6000 external fluorescent light source EL6000• External light source with liquid light guide• Adjustment-free metal halide lamp• Adjustable intensity of the excitation light

106 z lamp housing with:• 100 W high-pressure mercury burner (direct current, stabilized/nonstabilized, type 103 W/2)• Chromatically-corrected collector, focusable• Lamp mount can be centered• Supply unit ebq 100

Safety measures UV protection screen, UV light stop, stray light protection for lamp housing

Leica Z6 APO & Z16 APO / Z6 APO A & Z16 APO A Technical data, performance characteristics

5

Optical accessoriesObjectives Type/magnification factor Working distance Max. object field ∅

• Achromatic 0.32× 297 mm 112 mm

• Achromatic 0.63× 149 mm 56 mm

• Planapochromatic 0.5× 187 mm 70 mm

• Planapochromatic 0.8× 112 mm 44 mm

• Planapochromatic 1× 97 mm 35 mm

• Planapochromatic 2× 39 mm 17.5 mm

• Planapochromatic 5×/ 0.5 LWD 19 mm 5 mm

Objective adapters • For Leica M-series achromatic objectives• For 10× and 20× Leica DM objectives

DM objectives(only for upper zoom range)

• DM objective N Plan L 20× / 0.40 corr• DM objective N Plan 10× / 0.25– / A5.8

Fine focusing 10 mm travel, 1 micron resolution (Leica Z6 APO / Leica Z16 APO) (motorized and integrated for Leica Z6 APO A and Leica Z16 APO A)

Interpupillary distance 55 mm – 75 mm

Wide-field eyepieces for spectacle wearers

Widefield HC plan eyepieces 10×/20, 10×/22, 10×/25

ImagingImage acquisition, storage, and

processingVarious digital FireWire camera systems for fluorescence

Software for image archiving, analy-sis, and processing

LAS (Leica Application Suite), LAS AF (Leica Z6 APO A and Leica Z16 APO A motorized zooms), QWin, Leica MM AF powered by Metamorph®

Tubes • Binocular phototube HC L2TU 4 / 5 / 7 • Tube BD TP 25 100/50/0 with fixed port• Tube BD T 25+ V100/50/0, mechanical

Stands, illuminatorsTransmitted light stands Transmitted light bases TL ST, TL BFDF, TL RC™, and TL RCI™ for a variety of applications and illumination options

Incident light stand With stage plate, black and white, 120 mm

Motorized focus system With 620 mm column, control via handswitch, SmartTouch™, footswitch, or computer

Manual focusing drive Coarse/fine focus, adjustable ease of movement, with 620 mm column

Stages Leica IsoPro™ Motorized and Manual Cross-stage, gliding stage, Leica MATS Thermocontrol System heating stage, polarization

Additional illuminators Various LED illuminators and cold light sources

For additional accessories, see assembly diagram and scope of delivery.

66

Z6 APO / Z6 APO A: Zoom 0.57× – 3.6×Z16 APO / Z16 APO A: Zoom 0.57× – 9.2×

Optical data – with HC plan eyepieces 10× / 22Objectives

Working distances 97 mm 187 mm 112 mm 39 mm 20 mm

Eyepieces Total magnification Object field ∅ Total

magnification Object field ∅ Total magnification Object field ∅ Total

magnification Object field ∅ Total magnification Object field ∅

Zoom position × mm × mm × mm × mm × mm

0.57 7.1 30.9 3.6 61.8 5.7 38.6 14.3 15.4 35.6 6.18

0.8 10.0 22.0 5.0 44.0 8.0 27.5 20.0 11.0 50.0 4.40

1 12.5 17.6 6.3 35.2 10.0 22.0 25.0 8.80 62.5 3.52

1.6 20.0 11.0 10.0 22.0 16.0 13.8 40.0 5.50 100 2.20

2 25.0 8.80 12.5 17.6 20.0 11.0 50.0 4.40 125 1.76

2.5 31.3 7.04 15.6 14.1 25.0 8.80 62.5 3.52 156 1.41

3.2 40.0 5.50 20.0 11.0 32.0 6.88 80.0 2.75 200 1.10

3.6 45.0 4.89 22.5 9.78 36.0 6.11 90.0 2.44 225 0.98

4 50.0 4.40 25.0 8.80 40.0 5.50 100 2.20 250 0.88

5 62.5 3.52 31.3 7.04 50.0 4.40 125 1.76 313 0.70

6.3 78.8 2.79 39.4 5.59 63.0 3.49 158 1.40 394 0.56

8 100 2.20 50.0 4.40 80.0 2.75 200 1.10 500 0.44

9.2 (16 : 1) 115 1.91 57.5 3.83 92.0 2.39 230 0.96 575 0.38

6.3:1{

Objectives

Working distances 97 mm 187 mm 112 mm 39 mm 20 mm

Eyepieces Magnification chip : specimen

Magnification chip : specimen




Zoom position mm × mm mm × mm mm × mm mm × mm mm × mm

0.57 0.50 18.0 13.4 0.25 36.0 26.9 0.40 22.5 16.8 1.00 9.00 6.72 2.49 3.60 2.69

0.8 0.70 12.8 9.58 0.35 25.7 19.2 0.56 16.0 12.0 1.40 6.41 4.79 3.50 2.57 1.92

1 0.88 10.3 7.67 0.44 20.5 15.3 0.70 12.8 9.58 1.75 5.13 3.83 4.38 2.05 1.53

1.6 1.40 6.41 4.79 0.70 12.8 9.58 1.12 8.02 5.99 2.80 3.21 2.40 7.00 1.28 0.96

2 1.75 5.13 3.83 0.88 10.3 7.67 1.40 6.41 4.79 3.50 2.57 1.92 8.75 1.03 0.77

2.5 2.19 4.10 3.07 1.09 8.21 6.13 1.75 5.13 3.83 4.38 2.05 1.53 10.9 0.82 0.61

3.2 2.80 3.21 2.40 1.40 6.41 4.79 2.24 4.01 2.99 5.60 1.60 1.20 14.0 0.64 0.48

3.6 3.15 2.85 2.13 1.58 5.70 4.26 2.52 3.56 2.66 6.30 1.43 1.06 15.8 0.57 0.43

4 3.50 2.57 1.92 1.75 5.13 3.83 2.80 3.21 2.40 7.00 1.28 0.96 17.5 0.51 0.38

5 4.38 2.05 1.53 2.19 4.10 3.07 3.50 2.57 1.92 8.75 1.03 0.77 21.9 0.41 0.31

6.3 5.51 1.63 1.22 2.76 3.26 2.43 4.41 2.04 1.52 11.0 0.81 0.61 27.6 0.33 0.24

8 7.00 1.28 0.96 3.50 2.57 1.92 5.60 1.60 1.20 14.0 0.64 0.48 35.0 0.26 0.19

9.2 (16 : 1) 8.05 1.12 0.83 4.03 2.23 1.67 6.44 1.39 1.04 16.1 0.56 0.42 40.3 0.22 0.17

6.3:1{

Data with digital camera Leica DFC310 FX Full Frame

HC plan eyepieces 10× / 22, binocular phototube HC L2TU 4 / 5 / 7 (factor 1.25×), open iris diaphragm

Binocular phototube HC L2TU 4/5/7 (factor 1.25×), video objective 0.7×, open iris diaphragm

Planapochromatic 1× Planapochromatic 0.5×

Planapochromatic 0.5×



Planapochromatic 2×



Planapochromatic 5×Planapochromatic 1×

Object field projected onto chip





77

Leica MacroFluo™ with 6.3:1 zoom (Leica Z6 APO or Leica Z6 APO A)

Objective Planapochromatic 1× Planapochromatic 2× Planapochromatic HR 5×

Zoom range 7.1× – 45× 14.3× – 90× 35.5× – 225×

Resolution 60 – 351 Lp/mm 120 – 702 Lp/mm max. 1500 Lp/mm

Max. numerical aperture 0.117 0.234 0.5

Finest visible structure width 1.4 μm 0.71 μm 0.33 μm

Object field ∅ 4.9 – 30.9 mm 2.4 – 15.4 mm 1.0 – 6.2 mm

Max. depth of field 3.1 mm 0.8 mm 0.1 mm

Working distance 97 mm 39 mm 19 mm

Data with digital camera Leica DFC310 FX Full Frame / binocular phototube HC L2TU 4 / 5 / 7 (tube factor 1.25×) / video objective 0.7×

Magnification chip: Object 0.5× – 3.15× 1.0× – 6.3× 2.5× – 15.8×

Digital resolution 19.3 – 122 Lp/mm 35 – 220 Lp/mm 97 – 610 Lp/mm

Object field projected onto chip 18 × 13.4 mm / 2.9 × 2.1 mm 9 × 6.7 mm / 1.43 × 1.06 mm 3.6 × 2.7 mm / 0.57 × 0.4 mm


Data visual with binocular phototube HC L2TU 4 / 5 / 7 (tube factor 1.25×), eyepieces 10×/22

Objective Planapochromatic 1× Planapochromatic 2× Planapochromatic HR 5×

Zoom range 7.1× – 115× 14.3× – 230× 35.5 – 575×

Resolution 51 – 336 Lp/mm 102 – 672 Lp/mm max. 1500 Lp/mm

Max. numerical aperture 0.112 0.224 0.5

Finest visible structure width 1.49 μm 0.75 μm 0.33 μm

Object field ∅ 1.9 – 30.9 mm 0.96 – 15.4 mm 0.38 – 6.2 mm


Working distance 97 mm 39 mm 19 mm

Data with digital camera Leica DFC310 FX Full Frame / binocular phototube HC L2TU 4 / 5 / 7 (tube factor 1.25×) / video objective 0.7×

Magnification chip: Object 0.5× – 8.05× 1.0× – 16.1× 2.5× – 40.3×

Digital resolution 19.3 – 312 Lp/mm 38.7 – 624 Lp/mm 96.7 – 1500 Lp/mm

Object field projected onto chip 18 × 13.4 mm / 1.1 × 0.8 mm 9 × 6.72 mm / 0.56 × 0.42 mm 3.6 × 2.7 mm / 0.2 × 0.17 mm


Leica MacroFluo™ with 16:1 zoom (Leica Z16 APO or Leica Z16 APO A)

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Leica Z16 APO

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Coaxial incident light housing

Leica Z6 APO & Z16 APO Dimensions of zoom system, coaxial incident light housing

72.8∅58

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6

27.2

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8.4

3xM4, 6 mm depthInterface for Machine Mounting

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5

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13

Leica Z6 APO A & Z16 APO ADimensions of zoom systems

14

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LEICA 10 447 176PLANAPO 1.0× – WD 97 mm

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9

54.8


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612

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∅ 45

326

12

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M42 x 3 (P1)

LEICA 10 447 243PLANAPO 5.0× – WD 19.8 mm

∅48

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125

5.5

85.7

14.8

13.3

WD

= 19

.8

100

∅57 f7

∅ 45 mm

∅ 40 mm

8 mm

11 mm

6 mm

∅ 66 mm

M42(Fine Focus Interface)

M60(Achromat Objective Interface)

Dimensions of objectives, adapters

Adapter for achromatic objectives

15

17250

43

43

43

42°

187

188

5377

507

86.5

12

67

115

Adapter between fluorescent bridge and focus column

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leica macrofluo...macroscope with vertical beam path, motorized, apochromatic zoom system, incident...

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