leica em ace900 freeze fracture and etching › leica em ace900... · 2019-06-18 · leica em...
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Leica Mikrosysteme GmbH · Hernalser Hauptstrasse 219 · A-1170 WienT +43 488 99 · F +43 48899-350www.leica-microsystems.com
21.–22. Monat 20XXLEICA EM ACE900 A breakthrough in freeze
fracture
LEICA EM ACE900FREEZE FRACTURE AND ETCHING
CONNECT
WITH US!
Freeze fracture describes the technique of breaking a frozen
specimen to reveal internal structures. Freeze etching is the
sublimation of surface ice under vacuum to reveal details of
the fractured face that were originally hidden.
The Leica EM ACE900 is a modern instrument to perform
those tasks to the highest level of quality.
DISCOVER LEICA EM ACE900 UNIQUE FEATURES
CONNECTIVITY TO LEICA EM VCT500
WORKFLOW AND RELATED INSTRUMENTS
Leica EM ICE for high pressure
freezing with light stimulation
Leica EM VCM for sample
transfer to...
...Leica EM ACE900 to freeze
fracture and etch
...Leica EM VCT500 for shuttle
transfer to the cryo SEM
TEM analysis
A VCT500 dock is directly attached to the Leica EM ACE900.
Via the Leica EM VCT500 shuttle specimens are transferred
to and from the sample preparation instrument to be perfec-
tly preserved for the final examination in a cryo SEM. In this
way the fractured block face of a frozen sample is imaged
at highest resolution within a few hours between hydrated
sample and image analysis.
3-axis moveable cryo knife
Leica EM VCT500 transfer
Large cryo shields
High vacuum (10-8 mbar range)
Fast and easy e-beam coating with air locks
Bench top instrument
Touch screen interface
SEM TEM
Cryo-SEM analysis
Tilting & rotating cryo stage