legal protection for cryonics patients how brain cryopreservation research can contribute chana de...
TRANSCRIPT
![Page 1: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/1.jpg)
Legal Protection Legal Protection for Cryonics for Cryonics
PatientsPatientsHow brain cryopreservation How brain cryopreservation
research can contributeresearch can contribute
Chana de Wolf, M.S.Chana de Wolf, M.S.Advanced Neural BiosciencesAdvanced Neural Biosciences
![Page 2: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/2.jpg)
Strategies for Legal Protection of Cryonics Patients
Persuade the medical establishment that cryonics patients deserve legal protection because we believe so.
OR
Present evidence that cryonics patients are not dead according to contemporary criteria of death.
![Page 3: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/3.jpg)
Legal Criteria for Death
Cardio-respiratory death: irreversible cessation of heart beat and breathing.
Brain death: irreversible cessation of electrical activity in the brain.
![Page 4: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/4.jpg)
Brain DeathBrain Death
Accidental brain death: severe ischemic Accidental brain death: severe ischemic insults or head trauma.insults or head trauma.
Brain death as Brain death as choicechoice: contemporary : contemporary medicine gives up…..medicine gives up…..
BUT WE DON’T!BUT WE DON’T!
![Page 5: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/5.jpg)
How to Prevent Brain Death?
• Cryopreserve terminal patients and maintain them at cryogenic temperatures.
• Can this be done?
![Page 6: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/6.jpg)
Brain Cryopreservation Research
• First objective: good preservation of brain ultrastructure.
• Second objective: preserve viability of brain slices.
• Third objective: demonstrate maintenance of electrical activity in brain slices.
• Fourth objective: demonstrate maintenance of electrical activity in the whole brain.
![Page 7: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/7.jpg)
Ultrastructural Brain Preservation
![Page 8: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/8.jpg)
Viability in Brain Slices
• April 2006: Published survival of vitrified
brain slices (Pichugin, Fahy, et al).
– 100% viability of vitrified hippocampal
slices as measured by K+/Na+ ratios
• Slices also looked great when observed
by light and electron microscopy
![Page 9: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/9.jpg)
Electrical Activity in Brain Slices
• 2008: Recovery of electrical activity of vitrified brain slices when:– Immediately rewarmed after vitrification – Stored at -135C for 1-7 days– Vitrified using M22, VEG, or VM3
• Measured field EPSPs in response to stimulation were similar to control tissue– % responding and amplitude of response
• Persistence of the LTP “Memory Response” after vitrification and rewarming
![Page 10: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/10.jpg)
Electrical Activity in Whole Brain
• No public announcements to date.
• Objective: peer reviewed publication of maintenance of electrical activity after vitrification and rewarming.
![Page 11: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/11.jpg)
Cryonics Patients are NOT DeadCryonics Patients are NOT Dead
Public recognition of successful brain Public recognition of successful brain vitrification can be used to seek legal vitrification can be used to seek legal protection for cryonics patients.protection for cryonics patients.
We are not just saying these patients We are not just saying these patients are not dead, are not dead, mainstream criteriamainstream criteria do. do.
![Page 12: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/12.jpg)
How to Accomplish This?How to Accomplish This?
Support brain cryopreservation Support brain cryopreservation researchresearch
Aggressive PR efforts to communicate Aggressive PR efforts to communicate brain preservation research objectives brain preservation research objectives and achievements.and achievements.
Seek legal protection for cryonics Seek legal protection for cryonics patientspatients
Demand cryonics as an elective in-Demand cryonics as an elective in-hospital procedure.hospital procedure.
![Page 13: Legal Protection for Cryonics Patients How brain cryopreservation research can contribute Chana de Wolf, M.S. Advanced Neural Biosciences](https://reader038.vdocuments.us/reader038/viewer/2022110321/56649f4f5503460f94c716eb/html5/thumbnails/13.jpg)
Other Benefits of Brain Cryopreservation Research
Increased recognition of the technical feasibility of cryonics with scientists and the general public.
Improved cryopreservation procedures for existing members of cryonics organizations.