lecture 8 nitrogen
DESCRIPTION
GES175, Science of Soils. Lecture 8 NITROGEN. Slide 8.2. Slide 8.3. Slide 8.4. Oxidation States of Soil N. N Form Name Oxidation state organic-N-3 NH 4 + ammonium-3 â á N 2 dinitrogen gas 0 (oxidation) (reduction) NO 2 - nitrite+3 â á - PowerPoint PPT PresentationTRANSCRIPT
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Lecture 8Lecture 8
NITROGENNITROGEN
GES175, GES175, Science of SoilsScience of Soils
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Slide 8.2
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Slide 8.3
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Oxidation States of Soil NOxidation States of Soil N
N Form Name Oxidation state
organic-N -3
NH4+ ammonium -3
N2 dinitrogen gas 0 (oxidation) (reduction)
NO2- nitrite+3
NO3- nitrate +5
Slide 8.4
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Nitrogen Redox ProcessesNitrogen Redox Processes
Oxidation: loss of e- Reduction: gain of e-
-3 +5NH4
+ NO3-
8 e- transfer
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N-cycleN-cycleplant & animal residues
organic-N
NH4+NO2
-
NO3-
N2
1122
33
33
4455
55
Slide 8.6
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Mineralization vs. ImmobilizationMineralization vs. Immobilization
Fate of N if added to soil???
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Alfalfa, peas, grass
Low C:N (high N content)Slide 8.8
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straw, bark, sawdust
High C:N (low N) Slide 8.9
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Ammonia Volatilization Ammonia Volatilization - gaseous loss of N- gaseous loss of N
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Ammonia VolatilizationAmmonia VolatilizationUrea: CO(NH2)2 NH3 +CO2 + H2O
urea soil enzymes & H2O
- Most volatilization when:coarse or sandy-textured soils
low clay and low organic matter (which adsorb NH4
+)
dry alkaline surface
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NitrificationNitrificationNH4
+ NO2- NO3
- ammonium nitrite nitrate
- oxidation of N
* Autotrophic bacteria • obtain energy from N oxidation
• Nitrosomonas NH4
+ NO2- + energy
• Nitrobacter NO2
- NO3- + energy
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NitrificationNitrification (cont’d) (cont’d)
* Rapid in well-aerated, warm, moist soils
• aerobic organisms (O2 is required)
• little NO2- accumulation
* Acid-forming process NH4
+ +3/2O2 NO2- + 2H+ + H2O
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Nitrogen (nitrate?) Leaching
Eutrification
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Denitrification
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DenitrificationDenitrification
Gaseous loss of N upon N reduction
+ e- + e- + e- + e-
NO3- NO2
- NO N2O N2
nitric nitrous oxide oxide
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DenitrificationDenitrification (cont’d) (cont’d)
* Microorganisms responsible:
• facultative anaerobes - prefer O2 but will use N
for a terminal e- acceptor
• mostly heterotrophic - use organic-C for energy source
(reductions require energy)
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DenitrificationDenitrification (cont’d) (cont’d)
* Denitrification enhanced by:
• low O2 (flooding)
• high O.M. (energy source)
• high NO3-
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DenitrificationDenitrification (cont’d) (cont’d) * Metabolic reduction is not denitrification
(no N gas formation)
NO3-
organisms
NO3- NH4
+ organic-N
- N is reduced for use in protein formation
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Nitrogen FixationNitrogen FixationN2 (organisms) NH4
+
* Symbiotic relation between * Symbiotic relation between bacteria and plants:bacteria and plants:
- legumes- legumes + +
- - rhizobiumrhizobium
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Bacteria: Rhizobium genus (species specific)
R. meliloti - alfalfa R. trifolii - clover R. phaseoli - beans
- bacteria require plant to function
- inoculation of seed (coat seed with proper bacteria)
Nitrogen FixationNitrogen Fixation
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Process:Process:
Rhizobium
nodule
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(b) Process:(b) Process:
C from plant photosynthesis
N from fixation of N2 symbiosissymbiosis
RhizobiumRhizobium
organic-Corganic-C
NN22organic-Norganic-N
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Quantity of N FixedQuantity of N Fixed Alfalfa and clover provide 100 - 250 kg N/ha/yr
(mature stand, good fertility & pH)
Beans and peas less fixation but high protein food
with minimum N input
added N fertilizer lowered N fixation
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Symbiotic Nodules - NonlegumesSymbiotic Nodules - Nonlegumes
* Organismsactinomycetes - Frankia
* PlantsAlders and other trees
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Symbiotic - without nodulesSymbiotic - without nodules
* Azolla/Anabaena complex blue-green algae (N-fixer) in leaves floating fern in rice paddies
* Rhizosphere organisms use root exudates (C) large areas
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Nonsymbiotic N-fixation:Nonsymbiotic N-fixation:Free-living OrganismsFree-living Organisms
* Bacteria and blue-green algae
aerobic and anaerobic
small amounts: 5 - 50 kg/ha/yr
inhibited by available soil N