Lecture 07 Transcription Based on Chapter 5 Gene Expression: Transcription 1/29/2016 Copyright 2010 Pearson Education Inc. 1. Gene ExpressionThe Central Dogma: An Overview 2a. The Transcription Process 2b. The Transcription Process 3a. Transcription in Bacteria - Initiation of Transcription at Promoters 3b. Transcription in Bacteria - Initiation of Transcription at Promoters 4. Elongation of an RNA Chain 5. Termination of an RNA Chain 6. Transcription in Eukaryotes 7. Eukaryotic RNA Polymerases RNA polymerase I, located in the nucleolus, transcribes the three major rRNAs (28S, 18S, and 5.8S). RNA polymerase II, located in the nucleoplasm, transcribes mRNAs and some snRNAs. RNA polymerase III, located in the nucleoplasm, transcribes tRNAs, 5S rRNA, and the remaining snRNAs. 8. Transcription of Protein-Coding Genes by RNA Polymerase II 9. Promoters and Enhancers 10. Transcription Initiation 11a. The Structure and Production of Eukaryotic mRNAs 11b. The Structure and Production of Eukaryotic mRNAs 12. Production of Mature mRNA in Eukaryotes 13. 5 Modification 14. 3 Modification 15. Introns 16. Processing of Pre-mRNA to Mature mRNA 17. Coupling of Pre-mRNA Processing to Transcription and to mRNA Export from the Nucleus