lcd-array kit meat 5.0 - sensitivity - chipron · 2017. 8. 2. · certified according to din en...
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Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485
1 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
LCD-Array Kit MEAT 5.0 - Sensitivity
Mixtures of extracted animal DNA containing equal amounts of DNA (10 ng/µl) of each
species have been prepared from ‘Animal Control DNAs’ available at Chipron GmbH.
Three dilutions of these mixtures containing 1 ng/µl, 1 pg/µl and 100 fg/µl of each species’
DNA have been analyzed in eight replications each including one no template control (NTC)
per run.
Note: Since information of the exact genome size is not available for all species used in
these experiments, equal amounts of DNA have been mixed instead of preparing equimolar
mixtures. Due to different genome sizes (average mammals: ~3.3 pg/GE, average birds: ~1.3
pg/GE) the amount of DNA present in the dilutions does therefore reflect slightly different
numbers of genome equivalents (GE) depending on the respective species. Additionally, it
has to be considered that all DNAs have been extracted from fresh muscle tissue. As factors
like tissue type, animal age and even diet can affect the mitochondrial copy number per cell
as well as the mitochondrial genome copy number per organelle, the use of other tissue
samples than those used in this study can affect the assay sensitivity with respect to
absolute DNA amounts.
DNA Extraction & PCR:
Total DNA was extracted using the NucleoSpin Kit FOOD [Macherey & Nagel, Germany]
according to kit instructions. All samples were treated with RNase.
Nucleic acid concentration was measured with Nandrop 2000c® [Thermo Scientific,
USA] and all samples were adjusted to 100 ng/µl prior to preparation of mixtures A-D.
PCR and hybridisation was performed according to the instructions of the LCD-Array Kit
MEAT 5.0 using 5.0µl DNA / 25µl PCR and HotStar Taq Plus Mastermix® [Qiagen,
Germany] in a TProfessional® Thermal Cycler [Analytic Jena AG, Germany].
Scanning and Data analysis was done with the LCD-Array CHIP-Scanner PF7250u and
SlideReader Software Vers. 12 [Chipron GmbH, Germany] using the default cut off
settings for MEAT 5.0 arrays (cut off = 2.000 pixel values). Signals below 2.000 pv are
set to zero (0).
Tables on the following pages show the mean values of eight hybridizations. Refer to the
end of this report to see the entire data set.
Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485
2 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Stock Mix A Name Species ng / µl
Beef Bos taurus 10 Goat Capra hircus 10 Hare Lepus europaeus 10 Red deer Cervus elaphus 10 Springbok Antidorcas marsupialis 10 Mallard duck Anas platyrhynchos 10
1 ng/µl each species 1 pg/µl each species 100 fg/µl each species
Number of positives MEAN STDEV
Species 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl
Hyb-Ctrl 8 / 8 8 / 8 8 / 8 56502 56810 57326 2085 1774 1832
Cattle 8 / 8 8 / 8 8 / 8 57665 57525 33222 1787 2115 11834
Sheep 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Equine 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Goat 8 / 8 8 / 8 8 / 8 52771 51412 35804 2956 3327 6729
Camel 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Water Buffalo 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Pork 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Kangaroo 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Hare 8 / 8 8 / 8 8 / 8 55790 55909 56994 1961 2794 2774
Rabbit 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Reindeer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Roe deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Red deer 8 / 8 8 / 8 8 / 8 55724 54666 10018 2196 3979 6783
Fallow deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Springbok 8 / 8 8 / 8 8 / 8 50321 52965 46486 4021 3612 5750
Canine 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Cat 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Chicken 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Turkey 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Goose 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Ostrich 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Mallard duck 8 / 8 8 / 8 8 / 8 54932 56950 56973 1750 1926 2248
Muscovy duck 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Pheasant 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485
3 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Stock Mix B
Name Species ng / µl
Bison Bos bison 10 Buffalo Bubalus bubalis 10 Rabbit Oryctolagus cuniculus 10 Cat Felis silvestris catus 10 Chicken Gallus gallus 10 Muscovy duck Cairina moschata 10
1 ng/µl each species 1 pg/µl each species 100 fg/µl each species
Number of positives MEAN (pv) STDEV
Species 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl
Hyb-Ctrl 8 / 8 8 / 8 8 / 8 56378 55709 56670 2230 2062 2069
Cattle 8 / 8 8 / 8 8 / 8 57650 56670 58052 1750 1601 1814
Sheep 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Equine 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Goat 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Camel 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Water Buffalo 8 / 8 8 / 8 8 / 8 53961 56442 39700 4051 2057 9558
Pork 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Kangaroo 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Hare 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Rabbit 8 / 8 8 / 8 8 / 8 53360 49273 35254 2806 3231 9160
Reindeer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Roe deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Red deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Fallow deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Springbok 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Canine 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Cat 8 / 8 8 / 8 8 / 8 57614 57257 55178 2136 1565 4042
Chicken 8 / 8 8 / 8 8 / 8 48044 48101 11429 4134 3252 8046
Turkey 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Goose 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Ostrich 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Mallard duck 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Muscovy duck 8 / 8 8 / 8 8 / 8 54195 55643 47729 2508 2542 4704
Pheasant 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485
4 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Stock Mix C
Name Species ng / µl
Horse Equus caballus 10 Camel Camelus dromedarius 10 Pork Sus scrofa 10 Kangaroo Macropus rufus 10 Fallow deer Dama dama 10 Turkey Meleagris gallopavo 10 Pheasant Phasianus colchicus 10
1 ng/µl each species 1 pg/µl each species 100 fg/µl each species
Number of positives MEAN (pv) STDEV
Species 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl
Hyb-Ctrl 8 / 8 8 / 8 8 / 8 57929 57985 58189 1669 1547 1421
Cattle 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Sheep 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Equine 8 / 8 8 / 8 8 / 8 59277 58427 24336 1786 2720 11354
Goat 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Camel 8 / 8 8 / 8 8 / 8 55334 56213 55770 3002 2481 3183
Water Buffalo 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Pork 8 / 8 8 / 8 8 / 8 55048 47543 16191 2998 8914 4911
Kangaroo 8 / 8 8 / 8 8 / 8 58149 58073 59054 1480 1129 1558
Hare 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Rabbit 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Reindeer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Roe deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Red deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Fallow deer 8 / 8 8 / 8 8 / 8 48781 37707 8605 8177 6031 3998
Springbok 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Canine 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Cat 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Chicken 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Turkey 8 / 8 8 / 8 8 / 8 58127 58557 58581 1227 1394 2634
Goose 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Ostrich 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Mallard duck 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Muscovy duck 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Pheasant 8 / 8 8 / 8 8 / 8 56481 56832 57916 2492 3264 2965
Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485
5 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Stock Mix D
Name Species ng / µl
Sheep Ovis aries 10 Donkey Equus asinus 10 Reindeer Rangifer tarandus 10 Roe deer Capreolus capreolus 10 Dog Canis lupus familiaris 10 Goose Anser anser 10 Ostrich Struthio camelus 10
1 ng/µl each species 1 pg/µl each species 100 fg/µl each species
Number of positives MEAN (pv) STDEV
Species 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl 1 ng/µl 1 pg/µl 100 fg/µl
Hyb-Ctrl 8 / 8 8 / 8 8 / 8 58122 58518 58328 1677 1347 1389
Cattle 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Sheep 8 / 8 8 / 8 8 / 8 59699 56964 36294 1196 3296 7047
Equine 8 / 8 8 / 8 8 / 8 57409 40538 14699 2778 4602 3084
Goat 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Camel 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Water Buffalo 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Pork 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Kangaroo 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Hare 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Rabbit 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Reindeer 8 / 8 8 / 8 8 / 8 57331 35387 8040 2791 7672 2400
Roe deer 8 / 8 8 / 8 8 / 8 52216 38592 16902 4134 8849 3344
Red deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Fallow deer 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Springbok 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Canine 8 / 8 8 / 8 8 / 8 59359 57525 49255 1432 2572 7833
Cat 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Chicken 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Turkey 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Goose 8 / 8 8 / 8 8 / 8 55843 56578 57408 2475 3047 3584
Ostrich 8 / 8 8 / 8 8 / 8 55802 56678 52116 2905 3556 5032
Mallard duck 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Muscovy duck 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
Pheasant 0 / 8 0 / 8 0 / 8 0 0 0 0 0 0
6 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix A, 1ng/µl
No. Sample ID Mix A1 Mix A2 Mix A3 Mix A4 Mix A5 Mix A6 Mix A7 Mix A8 MEAN STDEV
1 Hyb-Ctrl 55951 52617 58939 57417 58408 54577 58580 55527 56502 2085
2 Cattle 56836 53936 58944 58106 59778 56952 59634 57137 57665 1787
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 53443 48502 55494 51056 56825 50712 56233 49906 52771 2956
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 56496 53318 58214 56590 56560 52595 58058 54486 55790 1961
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 57121 52660 57768 55433 58765 54800 56912 52336 55724 2196
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 51917 46518 56006 52002 54213 46653 51761 43499 50321 4021
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 54750 52531 56482 55216 57544 54361 56314 52258 54932 1750
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
Raw Data – Mix A, 1pg/µl
No. Sample ID Mix A1 Mix A2 Mix A3 Mix A4 Mix A5 Mix A6 Mix A7 Mix A8 MEAN STDEV
1 Hyb-Ctrl 57223 54555 59095 57135 57969 53737 58636 56132 56810 1774
2 Cattle 57602 54172 59004 56168 59036 54825 60562 58832 57525 2115
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 51889 47433 54474 49208 57262 50971 53189 46866 51412 3327
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 58826 55340 57810 54322 55664 49869 59204 56236 55909 2794
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 53605 48005 58717 55056 56316 49103 59850 56677 54666 3979
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 53464 48539 58206 54634 53998 46720 56503 51656 52965 3612
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 57993 55373 57702 56178 57405 53074 59889 57982 56950 1926
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
7 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix A, 100fg/µl
No. Sample ID Mix A1 Mix A2 Mix A3 Mix A4 Mix A5 Mix A6 Mix A7 Mix A8 MEAN STDEV
1 Hyb-Ctrl 58353 56948 59246 57254 57520 53097 59292 56897 57326 1832
2 Cattle 22254 18421 47691 42120 26800 20074 48512 39904 33222 11834
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 31908 26350 47108 41086 37190 28219 41651 32916 35804 6729
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 56784 53191 59924 57274 57806 52196 60660 58114 56994 2774
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 3752 2414 22283 16891 6359 3820 14656 9965 10018 6783
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 42949 37216 55684 50978 48598 41080 51206 44180 46486 5750
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 57649 53174 58272 55287 59884 54970 59877 56669 56973 2248
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
Raw Data – NTCs (Mix A)
No. Sample ID NTC A1 NTC A2 NTC A3 NTC A4 NTC A5 NTC A6 NTC A7 NTC A8 MEAN STDEV
1 Hyb-Ctrl 58529 57297 59632 58881 59089 56304 59444 56396 58197 1256
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
8 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix B, 1ng/µl
No. Sample ID Mix B1 Mix B2 Mix B3 Mix B4 Mix B5 Mix B6 Mix B7 Mix B8 MEAN STDEV
1 Hyb-Ctrl 56275 53352 58852 57145 56871 52452 59209 56871 56378 2230
2 Cattle 56568 54418 57756 57444 58678 56559 59360 60418 57650 1750
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 57794 55418 56762 55748 57362 53741 49244 45619 53961 4051
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 53822 49346 56294 53170 55112 49078 57380 52680 53360 2806
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 55182 53156 58901 58960 58742 57511 58622 59835 57614 2136
19 Chicken 49462 44166 50650 46116 48212 40830 55470 49446 48044 4134
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 52574 49565 56750 55632 56747 53726 56622 51946 54195 2508
25 Pheasant 0 0 0 0 0 0 0 0 0 0
Raw Data – Mix B, 1pg/µl
No. Sample ID Mix B1 Mix B2 Mix B3 Mix B4 Mix B5 Mix B6 Mix B7 Mix B8 MEAN STDEV
1 Hyb-Ctrl 56018 53352 57345 54362 56920 52370 59014 56293 55709 2062
2 Cattle 57094 54958 57301 55376 57782 54050 59186 57612 56670 1601
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 56630 53380 58274 54676 59490 55356 58756 54976 56442 2057
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 385 0 0 0 0 0 0 48 127
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 52282 47630 52738 47443 53856 46732 49600 43903 49273 3231
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 56600 54144 58416 56406 59277 56823 59022 57368 57257 1565
19 Chicken 49916 45058 49816 44690 51840 44250 53088 46152 48101 3252
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 54343 51222 57104 54106 58158 53944 59610 56654 55643 2542
25 Pheasant 0 0 0 0 0 0 0 0 0 0
9 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix B, 100fg/µl
No. Sample ID Mix B1 Mix B2 Mix B3 Mix B4 Mix B5 Mix B6 Mix B7 Mix B8 MEAN STDEV
1 Hyb-Ctrl 56730 53846 58372 55909 57821 53285 59639 57756 56670 2069
2 Cattle 58254 55854 59155 56964 58740 55046 60593 59808 58052 1814
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 43666 37633 51802 44992 49347 39714 28686 21756 39700 9558
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 26727 22806 51130 45973 39950 32564 34465 28418 35254 9160
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 56202 53140 60238 57720 53068 46650 59212 55194 55178 4042
19 Chicken 5280 3937 13177 10262 6508 4022 27144 21098 11429 8046
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 47782 43312 54074 50153 47076 39330 53900 46206 47729 4704
25 Pheasant 0 0 0 0 0 0 0 0 0 0
Raw Data – NTCs (Mix B)
No. Sample ID NTC B1 NTC B2 NTC B3 NTC B4 NTC B5 NTC B6 NTC B7 NTC B8 MEAN STDEV
1 Hyb-Ctrl 57174 55088 59107 57919 57554 54100 59074 56752 57096 1657
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
10 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix C, 1ng/µl
No. Sample ID Mix C1 Mix C2 Mix C3 Mix C4 Mix C5 Mix C6 Mix C7 Mix C8 MEAN STDEV
1 Hyb-Ctrl 56365 58031 56355 58397 55599 58052 60701 59928 57929 1669
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 55822 59661 57960 59988 57862 60495 61531 60895 59277 1786
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 53558 57660 53202 57046 49516 54522 59054 58114 55334 3002
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 51883 57181 53708 57570 49502 54540 58614 57384 55048 2998
9 Kangaroo 56910 59170 56673 58107 55982 58059 60356 59935 58149 1480
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 46484 54034 43789 51314 58224 60711 37967 37722 48781 8177
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 57314 59332 56010 58599 56738 58375 59760 58884 58127 1227
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 54028 57681 53504 57504 52818 57361 59790 59161 56481 2492
Raw Data – Mix C, 1pg/µl
No. Sample ID Mix C1 Mix C2 Mix C3 Mix C4 Mix C5 Mix C6 Mix C7 Mix C8 MEAN STDEV
1 Hyb-Ctrl 54977 57521 56871 58459 57526 58830 60372 59321 57985 1547
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 52526 57627 56541 60134 58168 60862 61084 60474 58427 2720
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 54628 57154 52447 56663 52856 57447 59543 58966 56213 2481
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 31185 40928 45366 53762 41792 50337 58923 58054 47543 8914
9 Kangaroo 55669 58692 56792 58650 58236 58798 59213 58532 58073 1129
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 33978 44575 31458 41880 38195 47912 31880 31780 37707 6031
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 55836 58809 57045 59458 58008 59903 60066 59330 58557 1394
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 49502 55370 55355 59138 56928 58683 60249 59428 56832 3264
11 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix C, 100fg/µl
No. Sample ID Mix C1 Mix C2 Mix C3 Mix C4 Mix C5 Mix C6 Mix C7 Mix C8 MEAN STDEV
1 Hyb-Ctrl 55812 58321 56684 58731 57424 58432 60456 59655 58189 1421
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 10574 16400 20407 27760 14464 20664 43192 41228 24336 11354
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 50107 56266 52872 57248 53054 57789 59709 59115 55770 3183
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 6977 11506 13432 19152 16206 22856 20274 19126 16191 4911
9 Kangaroo 56200 58872 57072 59224 59916 59568 61132 60450 59054 1558
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 8652 14284 5657 9602 9134 14448 3408 3656 8605 3998
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 54743 59576 56552 60110 54892 59492 61906 61378 58581 2634
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 52664 58135 53432 58271 59281 60223 60984 60338 57916 2965
Raw Data – NTCs (Mix C)
No. Sample ID NTC C1 NTC C2 NTC C3 NTC C4 NTC C5 NTC C6 NTC C7 NTC C8 MEAN STDEV
1 Hyb-Ctrl 57102 58128 58754 59616 58795 59046 60634 59780 58982 1008
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
12 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix D, 1ng/µl
No. Sample ID Mix D1 Mix D2 Mix D3 Mix D4 Mix D5 Mix D6 Mix D7 Mix D8 MEAN STDEV
1 Hyb-Ctrl 55653 57615 56575 59072 56749 58472 60787 60055 58122 1677
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 57096 59490 59464 60012 59544 59992 61702 60294 59699 1196
4 Equine 53458 58316 54804 59018 53660 59086 60891 60042 57409 2778
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 53955 58702 53232 58016 54472 59626 60794 59848 57331 2791
13 Roe deer 46404 53607 46064 53222 49217 56021 56968 56227 52216 4134
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 57896 60036 59167 60108 56430 59533 61123 60575 59359 1432
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 54109 58392 52816 56748 51894 55904 58897 57984 55843 2475
22 Ostrich 53367 57624 52088 55314 52286 56206 60140 59390 55802 2905
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
Raw Data – Mix D, 1pg/µl
No. Sample ID Mix D1 Mix D2 Mix D3 Mix D4 Mix D5 Mix D6 Mix D7 Mix D8 MEAN STDEV
1 Hyb-Ctrl 56844 58844 57059 59196 56956 58663 60661 59918 58518 1347
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 50477 56208 55156 59385 54485 59082 60628 60291 56964 3296
4 Equine 34200 43390 38828 48414 34962 45344 39843 39324 40538 4602
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 26906 35171 41338 52080 27258 35660 32642 32041 35387 7672
13 Roe deer 22480 30620 38582 48870 32105 41935 47564 46582 38592 8849
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 52044 56806 59701 60817 55975 59492 58160 57208 57525 2572
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 52326 57043 54589 58210 52277 57232 60710 60234 56578 3047
22 Ostrich 50300 55914 53231 57228 55230 59489 61254 60775 56678 3556
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
13 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]
Raw Data – Mix C, 100fg/µl
No. Sample ID Mix D1 Mix D2 Mix D3 Mix D4 Mix D5 Mix D6 Mix D7 Mix D8 MEAN STDEV
1 Hyb-Ctrl 56799 58648 56290 59170 56894 58793 60309 59720 58328 1389
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 25798 33421 34574 43244 27687 35607 45753 44266 36294 7047
4 Equine 11348 17213 9317 14089 12986 18659 17232 16745 14699 3084
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 3630 6297 6866 10860 6551 10129 10150 9838 8040 2400
13 Roe deer 10792 15996 15304 22122 14490 20500 18398 17610 16902 3344
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 36336 43241 55812 60166 52612 57232 45352 43289 49255 7833
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 49986 56130 56120 60061 55211 59438 61518 60801 57408 3584
22 Ostrich 42052 49212 49249 55573 50172 56610 57494 56566 52116 5032
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0
Raw Data – NTCs (Mix D)
No. Sample ID NTC D1 NTC D2 NTC D3 NTC D4 NTC D5 NTC D6 NTC D7 NTC D8 MEAN STDEV
1 Hyb-Ctrl 57066 58368 57622 59201 57056 58702 60186 59867 58509 1128
2 Cattle 0 0 0 0 0 0 0 0 0 0
3 Sheep 0 0 0 0 0 0 0 0 0 0
4 Equine 0 0 0 0 0 0 0 0 0 0
5 Goat 0 0 0 0 0 0 0 0 0 0
6 Camel 0 0 0 0 0 0 0 0 0 0
7 Water Buffalo 0 0 0 0 0 0 0 0 0 0
8 Pork 0 0 0 0 0 0 0 0 0 0
9 Kangaroo 0 0 0 0 0 0 0 0 0 0
10 Hare 0 0 0 0 0 0 0 0 0 0
11 Rabbit 0 0 0 0 0 0 0 0 0 0
12 Reindeer 0 0 0 0 0 0 0 0 0 0
13 Roe deer 0 0 0 0 0 0 0 0 0 0
14 Red deer 0 0 0 0 0 0 0 0 0 0
15 Fallow deer 0 0 0 0 0 0 0 0 0 0
16 Springbok 0 0 0 0 0 0 0 0 0 0
17 Canine 0 0 0 0 0 0 0 0 0 0
18 Cat 0 0 0 0 0 0 0 0 0 0
19 Chicken 0 0 0 0 0 0 0 0 0 0
20 Turkey 0 0 0 0 0 0 0 0 0 0
21 Goose 0 0 0 0 0 0 0 0 0 0
22 Ostrich 0 0 0 0 0 0 0 0 0 0
23 Mallard duck 0 0 0 0 0 0 0 0 0 0
24 Muscovy duck 0 0 0 0 0 0 0 0 0 0
25 Pheasant 0 0 0 0 0 0 0 0 0 0