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WHAT IS THE BOSCH LIVE CELL ANALYSIS FACILITY?
The Bosch Live Cell Analysis Facility (LCAF) is an open-‐access, multi-‐user facility that aims to provide all the necessary knowledge and instrumentation for its members to assess biological cell function.
WHAT EQUIPMENT IS AVAILABLE?
The LCAF provides expert training and access to live cell analysis and flow cytometry instruments, cryostats, a fluorescent microscope and several optical microscopes, and equipment for protein and nucleic acid analysis, tissue culture facilities, centrifuges and general laboratory equipment.
The LCAF also has a comprehensive Cell Bank that is available to registered users.
IX83 Motorised Live Cell Imaging System
The IX83 Motorised Live Cell Imaging System is a state-‐of-‐the-‐art live cell imaging system with its own incubator. It enables precise control of temperature; humidity and three gases to perform live cell imaging under both physiological and non-‐physiological conditions (such as hypoxia). The instrument is an automated imaging platform capable of area scanning, image stitching, and time-‐lapse imaging. The system includes 5 objectives and three LED cubes that allow detection of DAPI, GFP and mCherry/Texas Red.
This instrument is suitable for adherent and suspension cell cultures.
Becton Dickinson FACSCalibur Sort
The FACSCalibur is a standard flow cytometer with analytical and sorting capabilities. It is equipped with a blue and a red laser and four fluorescent detectors (FL). These detectors can recognise FITC (530/30 nm), PI or PE (585/42 nm), PerCP (670 nm LP), and APC (661/16 nm). The FACSCalibur is an easy to use instrument ideally suited for routine applications.
The LCAF has recently acquired a Beckman-‐Coulter Gallios, a 3-‐laser, 10-‐colour (12 parameter) flow cytometer.
iMac27-‐inch with FloJo Analysis Program
Tree Star FloJo is the leading paradigm in flow Cytometry data analyses. It is built on a special purpose data base, designed around the structure of flow data and the researcher’s experiments.
Olympus BX51 with DP70 camera
The OLYMPUS BX51 research microscope is a union of Olympus infinity corrected optical systems (UIS2 – universal infinity system) and advanced fluorescence or differential interference contrast DIC (Nomarski) technology. The Olympus DP70 is a 12.5 million-‐pixel cooled digital color camera system with 36-‐bits of RGB color depth.
Qiagene Rotor-‐Gene Q
The Rotor-‐Gene Q is a state-‐of-‐the-‐art innovative instrument that enables high-‐precision real-‐time PCR, end-‐point PCR, and high resolution melt (HRM) analysis. It has 6 channels that detect blue, green, yellow, orange, red, and crimson fluorophores. The Rotor-‐Gene Q uses a sophisticated heating and cooling design to achieve optimal reaction conditions. The unique rotary format ensures thermal and optical uniformity between samples, which is critical for precise and reliable analysis. The powerful and user-‐friendly software provides simplicity for beginners as well as an open experimental platform for advanced users.
LIVE CELL ANALYSIS FACILITY (LCAF)
CAS-‐1200 automated PCR set up
The CAS-‐100 is an easy to use robot developed specifically for the set up of real-‐time quantitative PCRs. The results achieved match or exceed the best hand pipetting. Set up is also fast; a 96-‐well plate takes about 20 minutes. The system is pre-‐configured to work with all PCR tube types.
Protein and Nucleic Acid Analysis
Gel electrophoresis equipment includes a Blot EZ Imager, Transblot Turbo and UV transilluminator.
Leica CM 3050 & 1510S Cryostats:
The LCAF has a Leica CM3050 cryostat located in the Anderson Stuart Bldg. A more modern cryostat (Leica 1510S) is also available in the LCAF lab in the Medical Foundation Bldg.
SpectraMax 190 Microplate Reader
The SpectraMax® 190 UV-‐Vis Microplate Spectrophotometer is a microplate reader for up to 96 samples with endpoint, kinetic and spectral scanning modes ideal for life science applications. The multi-‐channel design truly mimics a dual-‐beam spectrophotometer. Each sample has a discrete sample beam and reference beam so that each well is measured directly, eliminating error due to variations in light output between the optic fibers.
Cell Bank
The LCAF stores a wide variety of cell lines that are available to LCAF registered users.
WHERE IS THE LCAF LOCATED?
The LCAF is located in Rm G03 in the Medical Foundation Building (K25).
HOW DO I REGISTER WITH THE LCAF?
1-‐ Contact the LCAF officer 2-‐ Complete the LCAF registration form and pay the fees 3-‐ Arrange for training
WHAT TRAINING IS PROVIDED?
The LCAF not only provides instrument training, but also teaches the basic concepts necessary to understand the techniques used. The theoretical training is reinforced by one-‐on-‐one hands-‐on training sessions on the instruments.
WORKSHOPS
Mammalian Cell Culture: A Practical Approach, a course designed for beginners to understand the principles and practice of growing and maintaining mammalian cells in culture. It includes thawing, feeding, passaging and freezing cells.
Please contact the LCAF Officer for Services available.
HOW DO I CONTACT THE LCAF OFFICER? Shirley Nakhla Rm G07, Medical Foundation Building (K25) Ph: 903 63223 [email protected]
ACADEMIC ADVISER
Dr XiaoSuo Wang, [email protected]
For more information on the LCAF and other Bosch Institute Facilities visit:
http://sydney.edu.au/medicine/bosch/facilities/index.php
LIVE CELL ANALYSIS FACILITY (LCAF)