last prelab
DESCRIPTION
histo lapTRANSCRIPT
BENGHAZI UNIVERSITYFACULTY OF MEDICINE
DEPARTMENT OF HISTOLOGYFIRST YEAR MEDICAL PRELAB PART III
Prepared by:
Dr. Hala El.Alam .
Dr. Manal Eljerby.
Dr. Lobna Alferjani.
1
Prelab 9I Nervous tissue
Slides of lab 9..
1. Multipolar nerve cells in spinal cord. X402. Pyramidal nerve cells in cerebral cortex. X403. Pyriform nerve cells in cerebellum . X404. Astrocyte. X40
1.Multipolar nerve cells in spinal cord
1. Cell body (perikarion) : Pyramidal in shape .2. nucleus : Prominent nucleolus .3. Single axon .4. Multiple dendrites .
1.Multipolar nerve cells in spinal cord
Multipolar nerve cells in spinal cord x40
2.Pyriform nerve cells in cerebellum.
1. Called Purkinji cells.2. Multipolar cell.3. Arranged in raw between granular & molecular
layer .4. Flask shaped cell bodies .5. One axon &many branched dendrites
2.Pyriform nerve cells in cerebellum.
Pyriform nerve cells in cerebellum X40
Molecular layer
Granular layer
3.Pyramidal nerve cells in cerebral cortex X40
1. Large pyramidal cell body.2. Large vesicular nucleus.3. Multiple dendrites .
3.Pyramidal nerve cells in cerebral cortex X40
4.Astrocyte
1. Star shaped cell body.2. Large nucleus.3. Stained black with silver.4. Numerous long branched processes end with feet
like expansions on blood vessels.
4.AstrocyteAstrocyte
Astrocyte × 40.
Prelab 10 Nervous tissue II
Slides of prelab 10 ..
1. Oligodendrocyte . X402. Spinal ganglion . X40 3. Sympathetic ganglion . X404. Ependyma . X40 5. Nerve trunk . X40
1. Oligodendrocyte ..
1. small cell body .2. few processes .3. large dark nucleus . 4. form myelin sheaths in CNS .
Oligodendrocyte .. X40
Cell body
Dendrites
2&3 Spinal & sympathetic ganglion Spinal ganglion
1. Contain Pseudounipolar neurons.
2. Large with central nucleus.
3. In groups.
4. Completely surrounded by satellite cells.
5. Separated by thick myelinated fibers.
6. Oval shape ,Thick capsule.
7. They are present beside spinal cord in both sides .
Sympathetic ganglion
1. Multipolar neurons.
2. Small numerous with eccentric nucleus.
3. Scattered.
4. Have few less prominent satellite cells.
5. Separated by Unmyelinated nerve cells.
6. Round shape ,Thin capsule.
7. Related to autonomic nervous tissue .
Spinal & sympathetic ganglion
Spinal ganglion Sympathetic ganglion
2.Spinal ganglion … X40
C.T capsule
Pseudounipolar N .
Myelinated n. fibers
Stellate cells
Spinal ganglion X100
Pseudounipolar neuron
Central nucleus &prominent nucleolus.
Stellate cells.
Spinal ganglion X40
3.Sympathetic ganglion… X40
Multipolar N.
Unmyelinated N. fibers
4.Ependyma..
1. single layer of simple cuboidal epithelium .
2. lines the ventricles of brain & central canal of spinal cord .
3. Ciliated and non ciliated .
4.Ependyma..
Ependyma x40
Cilia
5.Nerve trunk ..
1. . Nerves are surrounded by epineurium . 2. Each bundle (or fascicle) of nerve fibers is surrounded by
perineurium .
3. Each nerve fiber within each bundle is individually surrounded by endoneurium
5.Nerve trunk..
Nerve trunk .. X40
Perineurium
Endoneurium
Nerve axon
Prelab 12Lymphatic tissue
Slides of prelab
1. Thymus gland . X40.2. Lymph node . X40.3. Spleen . X40.4. Palatine tonsils . X40.5. Payers patches . X40.
Thymus
Hassall corpuscle
Thymus
a- Capsule and septa of C.T tissue b- Cortex and medullac- Medulla contains Hassall's corpuscles
Lymph node
• C.T capsule and Trabeculae
• b- Cortex : lymphatic nodules with germinal center
• c- Medulla : Medullary cords and sinuses.
Lymph node
cortex
Medulla
Spleen
• a - C.T capsule and Trabeculae• b- White pulp ( lymphatic nodules & central artery)• c-Red pulp (splenic cord and splenic venous sinuses)
Spleen
Spleen
Spleen
White pulp1. Lymphatic
nodule with germinal center.
2. Central artery .
Palatine Tonsils
• Tonsillar crypts.• Lymphatic nodules• Stratified nonkeratinized epithelial covering.
Palatine Tonsils
• Tonsillar crypts.• Lymphatic nodules• Stratified nonkeratinized epithelial covering.
Palatine Tonsils
Crypt
Lymphatic nodules
Mucus glands
Epithelium
Palatine Tonsil
Peyer`s Patches
• Are aggregations of lymphatic nodules.
• Located in the lamina propria of the wall of the ileum opposite to the mesentery.
Payer’s Patch
Payer’s Patch
Prelab 13Skin
Slides for the prelab
• 1. thick skin x10• 2. thin skin x10• 3. hair follicle ( cross section) x40• 4.negroid skin x40.
1.In palms & soles.
2 Keratinized stratified squamous epithelium.
3.Thick epidermis.
4.Many sweat gland.
5.No hair or sebaceous gland.
Thick skin
Thick Skin
Thin skin1- Epidermis : thin. 2- Dermis :thick 3- Stratified squamous Keratinized epithelium 4- Hair follicles5- Sebaceous and sweat glands
Thin skin
Thin skinepithelium
Errector Pillae muscle
Sebaceous gland
Hair follicle
Sweat gland
Thin skin x10
Thin skin x10
Hair follicle (cross section)
1.Cortex 2.medulla 3. inner & outer root sheet. 4.connective tissue sheet.
Hair follicle (cross section) x40
Negroid skin1. Stratified squamous Keratinized epithelium.
2. Melanin pigment
Negroid skin X40
Negroid skin X40
Differences between thick & thin skinThin Skin Thick Skin
Whole body except palms and soles
Palms & soles Site
Thinner Thicker Epidermis
Present Absent Hair follicles & sebaceous glands
Not characteristic. Characteristic (finger prints)
papillary ridges
Prelab 14Vascular system
Slides of this lab• 1. aorta (as an example of
large artery).
• 2.inferior vena cava(as an example of large vein)
• 3.medium sized artery& vein.
1- Tunica intima: endothelium and subedothelium connective tissue.
2.internal elastic lamina. 3- Tunica media: thick, mainly of elastic fiber with smooth muscle cells.
4- Tunica adventitia: Thin and is composed of loose fibroelastic connective tissue, contain vasa vasorum.
Aorta
Aorta
aorta
Veins (Inferior vena cava)
1.Tunica intima: Thin, endothelium and subedothelium.
2.Tunica media: Thin and contain few smooth muscle fiber.
3.Tunic adventitia: Thickest of the three tunicas, contains coarse collagen fibers, elastic fibers and longitudinal bundles of smooth muscle.
4. no internal or external elastic lamina
Inferior vena cava
Inferior vena cava
Medium sized artery Medium sized vein
wall Thick with narrow lumen
Thin with wide lumen .
Tunica intima thick folded with internal elastic
lamina
thin
Tunica media thick, formed of circularly arranged
smooth muscles separated by
Collagenous fibers, and few elastic
connective tissue
Thin, thin and formed of smooth muscle cells
Tunica adventitia thin thick
Internal elastic lamina
Well developed absent
External elastic lamina
May be present absent
Medium sized artery and vein