Supporting all major next-gen sequencing workflows and

technologies GENOMICS

• Reference guided genome and exome assemblies

• De novo genome assembly• Targeted sequencing and gene

panels• Cancer genomics• Metagenomics• Variant detection accuracy

>99%• Copy number variation (CNV)

calculation

TRANSCRIPTOMICS

• De novo transcriptome assembly with auto-mRNA annotation

• RNA-Seq gene expression analysis

• ChIP-Seq peak detection• Microarray analysis• miRNA discovery and

quantification

INTEGRATED DATA ANALYSIS AND VISUALIZATION

• View and analyze genomic variation, gene expression and gene regulation data in a single project

• Genome-scale, multi-sample analysis and browsing for any project type

• Sanger validation of NGS assemblies and variant calls

• Viral-host integration analysis

Supported Sequencing Platforms:

Genomics SuiteLASERGENE®

Scatter plot comparing gene expression values for RNA-Seq data

Alignment view for multi-sample assembly

Sashimi plot illustrating differential exon expression

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Lasergene Genomics Suite offers the most accurate, fastest, and easiest-to-use pipeline for next-gen sequence assembly, alignment, and variant calling.

Accuracy and Speed Benchmarks

Operating SystemsWindows

Macintosh

Hardware Requirements16-32 GB RAM

Quad-Core 3 GHz processor

Two 1-4 TB hard drives (for reference-

guided alignments)

0.808.234.1643 U.K.0.800.182.4747 Germany

REFERENCE-GUIDED ALIGNMENT BENCHMARKS

Data Set Sequence Technology Coverage Assembly Time

Yeast Genome Illumina 22X 2 minutes

Yeast Genome Ion Torrent 22X 3 minutes

Drosophila Genome Illumina 23X 23 minutes

Human Exome Illumina 81X 53 minutes

Mouse RNA-Seq Ion Torrent Proton NA 1.3 hours

Human RNA-Seq Illumina NA 1.6 hours

Rice Genome Illumina 34X 1.6 hours

Human Exome Ion Torrent Proton 162X 3.9 hours

ACCURACY COMPARISONS FOR HUMAN EXOME VARIANT ANALYSIS USING NA12878

Workflow Sensitivity SpecificityFalse

Discovery Rate

True Positives

False Positives

False Negatives

Elapsed Time

Lasergene Genomics Suite 99.56% 99.999% 1.29% 15,272 200 67 1.3 hr

CLC Bio’s Genomics Workbench 8.0 99.18% 99.995% 7.41% 15,553 1,245 288 3.1 hr

Geneious 8.1 91.68% 99.995% 7.82% 14,827 1,257 1,346 2.9 hr

BWA Mapper /GATK Unified

Genotyper99.09% 99.999% 1.08% 15,161 166 139 6.0 hr

BWA Mapper /GATK Haplotype

Base Caller99.14% 99.999% 0.97% 15,168 149 132 6.3 hr

DE NOVO TRANSCRIPTOME ASSEMBLY BENCHMARKS

Data Set Sequence Technology

Number of Reads (Millions)

TranscriptsAverage Transcript

Length

Assembly Time

Human Illumina 100 30,342 975 15 hours

Water Bear Illumina 45 24,960 1,680 30 hours

Genomics SuiteLASERGENE®

info@dnastar.com

Multiple genome assemblies

Venn diagram used to compare SNPs, genes & peaks