laser microdissection case study
DESCRIPTION
Laser microdissection case study. MCB 7300. Laser-Capture Microdissection , a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize. - PowerPoint PPT PresentationTRANSCRIPT
Laser microdissection case study
MCB 7300
Laser-Capture Microdissection, a Tool for the Global Analysis of Gene
Expression in Specific Plant Cell Types:
Identification of Genes Expressed Differentially in Epidermal Cells or
Vascular Tissues of Maize
Mikio Nakazono, Fang Qiu, Lisa A. Borsuk and Patrick S. SchnableThe Plant Cell, Vol. 15, No. 3 (Mar., 2003), pp. 583-596
Overview
• RNA isolation from different plant cell types for microarray analysis
• Protocol changes: cryoprotectant; coagulating fixative (ethanol:acetic acid)
Yields