ladislav nedbal department of biological dynamics institute of systems biology & ecology cas...
TRANSCRIPT
![Page 1: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/1.jpg)
Ladislav NedbalDepartment of Biological Dynamics
Institute of Systems Biology & Ecology CASZámek 136, 37333 Nové Hrady, Czech Republic
![Page 2: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/2.jpg)
Solar biofuels from microorganisms, why not yet?
0 < (EFUEL –EM) / m2.year = (EEsolar - EM) / m2.yearEM… construction, maintenance, fertilizers, pumping, harvesting,
processing, refining, distribution…)
EM … biological and technological constraint (≈ € !!!)
Esolar … geographical constraint
CROP Corn Soybean Canola Jatropha Coconut Oil palm Microalgae Microalgae
OIL YIELD (l/ha)
172 446 1190 1892 2689 5950 136900 58700
70% and 30% oil in wet biomass (adapted from Y.Chisti (2007) Biotechnology Advances 25: 294-306)
Region SW USA NE USA UK
Lipid content 0.5 0.33 0.5 0.33 0.5 0.33
Biodiesel, gal/m2/year
1.24 0.82 1.06 0.71 0.77 0.52
With yields ≈ 1-2 gal(oil) / m2 / year and oil price < $3 / gal: the capital cost depreciation < several $ / year
![Page 3: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/3.jpg)
Solar biofuels from microorganisms … continued …0 < (EFUEL –EM) / m2.year = (EEsolar - EM) / m2.year
EM… construction, maintenance, fertilizers, pumping, harvesting, processing, refining, distribution…)
EM … biological and technological constraint (≈ € !!!)Esolar … geographical constraint
E … thermodynamic, biological a technological constraint
Irradiance
Used
LostSolutions:- Decrease the antenna- Dilute the light technologically in space
- Dilute the light technologically in time
![Page 4: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/4.jpg)
High density algal cultures
![Page 5: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/5.jpg)
Cell light dynamics in dense algal cultures
![Page 6: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/6.jpg)
Light dynamics in dense algal cultures
A.A.
B.B.
C.C.
![Page 7: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/7.jpg)
Constant mean irradiance, variable period & L:D ratio
BB
Chlorella vulgaris500 E.m-2.s-1
![Page 8: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/8.jpg)
Constant incident irradiance, variable period & L:D ratio
AA
Chlorella vulgarisI0=2000 E.m-2.s-1
1ms
continuous
100ms
1s
![Page 9: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/9.jpg)
Constant incident irradiance, variable L:D ratio
AA
Chlorella vulgaris10 ms period
2400 E.m-2.s-1
1200
700
250
130
![Page 10: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/10.jpg)
Constant incident irradiance, constant L:D, variable period
Chlorella vulgarisL:D=1:1, I0=2000 E.m-2.s-1
2400 E.m-2.s-1
1200
700
250
130
T
![Page 11: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/11.jpg)
Rate of photoinhibition
L:D=1:1, I0=2000 E.m-2.s-1
T= 10ms
Cont. I0=1000 E.m-2.s-1
![Page 12: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/12.jpg)
Electron transfer
![Page 13: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/13.jpg)
Conclusions for flashing light effect in the ms-range:
1) per area yield increase in dense cultures & high irradiance
2) reduction of photoinhibition rates
![Page 14: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/14.jpg)
![Page 15: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/15.jpg)
Harmonic light forcing with periods > 1 s
harmonic light input
non-linear output
2 amplitudeT
offset
![Page 16: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/16.jpg)
Simulation tool
Nedbal et al.(2008) Biotechnology & Bioengineering 100: 902-910.
![Page 17: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/17.jpg)
Non-linear fluorescence responseContinuous light Oscillating light
Nedbal et al. (2003) Biochim.Biophys.Acta: Bioenergetics 1607: 5-17Nedbal, et al. (2005) Photosynth.Res. 84: 99–106
![Page 18: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/18.jpg)
Non-linear response in dynamic light
![Page 19: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/19.jpg)
Non-linear response caused by regulation
![Page 20: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/20.jpg)
Non-linear response caused by regulation
![Page 21: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/21.jpg)
Non-linear response caused by regulation
![Page 22: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/22.jpg)
Non-linear response in growth C
onst
ant l
ight
T=10
s
T=10
0s
Con
stan
t lig
ht
![Page 23: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/23.jpg)
![Page 24: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/24.jpg)
Metabolic rhythms of the cyanobacterium Cyanothece sp. ATCC 51142 correlate with modeled dynamics of circadian clock.
![Page 25: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/25.jpg)
Different L/D ratio.
![Page 26: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/26.jpg)
Different L/D ratio.
![Page 27: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/27.jpg)
Model prediction for circadian Kai clock confronted with metabolic dynamics in 12L: 12D
![Page 28: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/28.jpg)
Model prediction for circadian Kai clock in 6L: 6D
![Page 29: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/29.jpg)
Model prediction in 6L: 6D qualitatively confirmedby experiment
![Page 30: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/30.jpg)
Modeling Tools
![Page 31: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/31.jpg)
Models are able to simulate the non-linearity … poorly
![Page 32: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/32.jpg)
Comprehensive modeling space, SBML, MIRIAM
![Page 33: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/33.jpg)
…. soon to be continued
![Page 34: Ladislav Nedbal Department of Biological Dynamics Institute of Systems Biology & Ecology CAS Zámek 136, 37333 Nové Hrady, Czech Republic](https://reader036.vdocuments.us/reader036/viewer/2022081511/56649e535503460f94b48e36/html5/thumbnails/34.jpg)
Thank you for your attention!
Collaborators:
Photon Systems Instruments, Brno, CZ
Jan Červený, Ondra Komárek, Víťa Březina from
Nové Hrady, CZ
Fusheng Xiong, Vláďa Tichý from Třeboň, CZ
Johann Grobbelaar, UOFS Bloemfontain, S.Africa
Himadri Pakrasi, WUSTL, St.Louis, USA
Govindjee, UIUC, Urbana, USA
Agu Laisk, Tartu, Estonia
Conrad Mullineaux, London, UK