lacz lacy laca oa i repressor lactose lactose/repressor rna pol crp camp crp/camp complex cap...

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lacZ lacY lacA O A i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operato r activator Regulatory gene P lacZ lacY lacA O A i NO transcription lacZ lacY lacA O A i NO transcriptio n lacZ lacY lacA O A i Transcription occurs lacZ lacY lacA O A i Lac operon

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Page 1: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator

lacZ lacY lacAOAi

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

B-galactosidase

lactose permease

transcetylase

operatoractivatorRegulatory gene

P

lacZ lacY lacAOAi

NO transcription

lacZ lacY lacAOAi

NO transcription

lacZ lacY lacAOAi

Transcription occurs

lacZ lacY lacAOAi

Lac operon

Page 2: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator

Not taken up

Lots of glucose

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

IIC

glycolysis

Glucose

PEP

Pyruvate

Kreb’s

IIB

HPr

IIA

EI ATP

glucose

glucose

lactose

Low cAMP

Page 3: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

IIC

glycolysis

Glucose

PEP

Pyruvate

Kreb’s

HPr

IIA

EI ATP

IIB

lactose

Adenylate cyclase

Not more glucose

This process slows down

Levels of cAMP increase

Page 4: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

IIC

glycolysis

Glucose

PEP

Pyruvate

Kreb’s

HPr

IIA

EI ATP

IIB

lactose

Adenylate cyclase

Not more glucose

This process slows down

lacOp becomes active

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230 plaque crp or myocardial crp

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Identification Tetracycline Resistance Promoter Repressor

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DNA lacZ lacI O lacY lacA C E P rep T CAP repressor CAP site pol-binding site operator structural genes terminator lac operon lactose cAMP 8

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CRP-123 CRP-129 en.desbloqueado55 - Copia