The suite is equipped with a high pressure homogenizer (Aves-tin) which will allow the disruption of dissimilar samples with a minimum maintenance and a low cost. The equipment has a built-in compressed air supply (100 psi) which will allow to pro-cess different samples: liposomes (70 psi), E. coli (70 psi) and Pichia (100 psi). The installed cooler of the equipment, con-nected to tap water, keeps the temperature low during sample disruptions. The suite is also equipped with Dyno-Mill cell dis-ruptor to process yeast samples. The equipment is fitted with a circulating bath which allows the disintegration of samples at low temperatures. For small volume of samples, BeatBeater is also available.

The suite is equipped with one large-scale fermentation system (70-L stainless steel bioreactor). A steam generator is installed for one-step in-suite sterilization. It has an Applikon bio-controller (Model ADI 1010) and Pilot system (ADI 1075) with three pneumatic pumps (acid, base, and antifoam), stirrer controller, and rotameters, which provide user-friendly setup and automated control/recording of temperature, pH, dissolved oxygen (dO2) during the fermentation process. One computer is installed with BioXpert software for large-scale fermentation.

The suite is equipped with six small-scale fermentation systems (three with 3-L bioreactors and three with 7-L bioreactors) available for Escherichia, Streptomyces, Rhodobacter, Salmonella, Bacillus and others. Each fermentation system has an Applikon bio-controller (ADI 1030), stirrer controller (ADI 1032), three rotameters (air, O2, N2), and three pneumatic pumps (acid, base, antifoam/label), which allow automated control/recording of temperature, pH, and dissolved oxygen (dO2) during the fermentation process. One computer is installed with BioXpert software for bacterial fermentation.

Bacterial Fermentation System (3-L or 7-L)

The suite is equipped with a CEPA high speed centrifuge to harvest large volume of cells and media. It allows the collection of 50 liters of media within 45 minutes. The sample port of the 70-L fermenter can be directly connected to the high speed centrifuge to allow the harvest of samples after the fermentation process.

CEPA Z41 High Speed Centrifuge

Eukaryatic Fermentation System (3-L or 7-L)

Cell Disruption Equipment

Among the six small-scale fermentation systems, three of them are also equipped with individual methanol (MeOH) detectors and sensors from Raven Biotech Inc. and pumps for automatic control/recording of MeOH level in the media during Pichia fermentation.

Large-scale Fermentation System (70-L)

One computer is installed with BioXpert software for Pichia fermentation. The suite also has one separate bioreactor (3-L) with marine type impeller and sparger suitable for animal cell culture.

The suite is equipped with one DASGIP OD4 module and three DASGIP Optical Density Probes, 12 mm diameter, 225 mm inser-tion length w/ 5 mm light path. Easy access software (DASGIP) is installed in all computer system for recording.

DASGIP OD4 module

Labnet incubator shaker for Pichia culture

The suite is equipped with one Labnet Incubator Shaker (I5311-DS) for plate incubation and small scale (50-ml flask or 50-ml falcon tubes) culture exclusively for Pichia cells.

Rm 4303 (Office), Rm 4332 (Lab)The University of British Columbia

4th Floor, Life Sciences Centre2350 Health Sciences Mall

Vancouver, BC V6T 1Z3 Canada

Suite Manager : Sung-Hye Grieco, Ph.D.Tel: 604-827-4356 (Office)

Tel: 604-836-6774 (Cell)Email: sunghye@interchange.ubc.ca

Web: http://www.cbr.ubc.ca/fermenter.htm

CBR Fermentation Suite

Fermentation Database is available:From June 2008, we started an on-line database system to download results and invoices and also for data analysis and comparison. Please visit our web site www.cbr.ubc.ca/fermenter.htm to see detail.

1. Mention us in Acknowledgement Section as: “The authors thank the Centre for Blood Research Fermentation Suite to provide fermentation equipment.”

From Jan 2011, the suite started this fermentation optimization package to help finding optimal condition to maximize produc-tion in fermentation. Design of Experiment (DOE) is a widely used method in process optimization in chemical engineering and industry. Optimization package provided from our suite is for 3 factors (such as temperature, pH, or dissolved oxygen) with 20 runs of small scale (700 ml) fermentations. It requires 7 days of experiments (3 fermentations per day) to achieve data. This service provides data analysis using DOE software to find a fit model for each responses and optimal condition for the re-quirement (goals). For further assistant, please contact suite manager at sunghye@interchange.ubc.ca.

Fermentation Optimization Package (NEW)

Fermentation Annual Workshops Pichia workshop provides hand-on experiments for handling bioreactors and pichia culture. It provides detailed theory as well as experimental know-how by suite manager. Pichia pasto-ris strains are used to produce human Cathepsin K (with coop-eration from Dr. Dieter Bromme lab) for easy assay for produc-tion estimation. DOE workshop provides basic statistical introduction about Design of Experiment software from Stat-East Inc. Company and basic hands-on experience for handling bioreactor as well. Design experiment for optimization, conducting fermentation, and data analysis will be discussed during the workshop. These workshops are not credited courses nor certification but for training purposes. Each workshop will be announced via LSI Email list.

Publication Guideline

This brochure is updated at Feb 2011