lab one: a beginner’s guide to the spikerbox
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Lab One: A beginner’s guide to the spikerbox. Brain Cells need to communicate with each other to control the body . A brain with only one neuron is not a brain. A brain is a network (friendship) of neurons. How do neurons talk?. Method 1: Chemical communication e.g. Bacteria - PowerPoint PPT PresentationTRANSCRIPT
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Lab One: A beginner’s guide to the spikerbox
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Brain Cells need to communicate with each other to control the body
A brain with only one neuron is not a brain
A brain is a network (friendship) of neurons
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How do neurons talk?
Method 1: Chemical communicatione.g. Bacteria
Con: slow, limited by diffusion
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How do neurons talk?
Method 2: Chemical communication through synapsee.g. stretching the neuron to cover more space
Con: still slow, signal must travel long way through cell
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How do neurons talk?Method 3: Electrical and chemical combination
Electricity carries signal quickly down long axon to synapse, chemical transmission at synapse to the next neuron
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Electrical Neural Communication: The Action Potential
Action potentials (“spikes”) are generated by Na infusion depolarizing the cell membrane and K expulsion repolarizing it.
Spike “moves” as depolarization propagates down axon
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Wikipedia.org
Anatomy of the Neuron
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Model of the nervous system: Discoid cocroach (Blaberus discoidalis)
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Neuroanatomy of Cockroach
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Neuroanatomy of Cockroach Leg
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Procedures: Put the cockroach in a jar of ice water
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Procedures: Cut off one of roach’s legs near the body
(Put petroleum jelly on exposed wound on body and leg, return cockroach to house, leg will grow back if cockroach not full grown adult)
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Procedures: Place leg on cork of SpiderBox, with a bit of leg
overhang
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Procedures: Put one electrode in the femur and the other in the
coxa
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Procedures: Observe the spike trains, demonstrating neuron
communication
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Classify spikes by amplitude: binning
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Entering Your DataCondition Method Notes Timing Notes
No Stimulation
Toothpick Stimulation
Blowing Through Straw