Lab Meeting

10/04/2013

Results – Theophylline Disc

Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA

High Promoter Low PromoterConstructs on same Plasmid

Results – Caffeine Disc

Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA

High Promoter Low PromoterConstructs on same Plasmid

Results 15 hour absorbance minus initial absorbance with 1 mM Theophylline

high promote

r + ecd

m8 (low co

py) + te

tA m

odule (high co

py)

low promote

r + ecd

m8 (low co

py) + te

tA m

odule (high co

py)

tetA

module (lo

w copy)

tetA

module (h

igh copy)

ecdm8 + te

tA m

odule 0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

NO TETRACYCLINELOW TETRACYCLINEHIGH TETRACYCLINE

cell

deni

sty

Results 15 hour absorbance minus initial absorbance with 5 mM Caffeine

high promote

r + ecd

m8 (low co

py) + te

tA m

odule (high co

py)

low promote

r + ecd

m8 (low co

py) + te

tA m

odule (high co

py)

tetA

module (lo

w copy)

tetA

module (h

igh copy)

ecdm8 + te

tA m

odule -0.05

0

0.05

0.1

0.15

0.2

NO TETRACYCLINELOW TETRACYCLINEHIGH TETRACYCLINE

cell

deni

sty

0mM of Theophylline

Ecdm8(low copy) + TetA Module(high copy)

0.05mM

0.4mM 0.2mM

0.1mM

Ecdm8+ TetA Module

0.05mM

0.4mM 0.2mM

0.1mM

0mM of Theophylline

Future work

• Test tetA fitness module and ecdm8 with caffeine, 3-methylxanthine, and xanthine

• Thesis Preview Tuesday common hour Dana 146