Gene$c  varia$on  in  resistance  to  Pacific  oyster  mortality  

syndrome  Peter  Kube  (CSIRO)  

Ma?hew  Cunningham  (ASI)  Mike  Dove  (NSW  DPI)  

Wayne  O’Connor  (NSW  DPI)  Paul  Hick  (NSW  DPI)  

Presenta$on  to  Shellfish  Futures  2012  17-­‐19  August  2012  Port  Arthur  Tasmania  

Tray  1  

Tray  9  

Woolooware Bay, Georges River (24 May 2012)

ASI  gene$cs  trial   University  of  Sydney  trial  

Large  varia$on  in  survival  along  rack  34

%

32%

33%

20%

17%

14%

33%

32%

20%

43%

43%

43%

71%

37%

54%

69%

58%

53%

49%

62%

74%

58%

82%

78%

51%

56%

61%

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

AVERAGE  SURVIVAL  =  47%  Survival  aZer  14  days  (26  April  2012)  

Tray  1  

Tray  9  

Some  varia$on  in  survival  across  rack  

AVERAGE  SURVIVAL  =  47%  Survival  aZer  14  days  (26  April  2012)  

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

4 4 %

4 4 %

4 6 % 4 6 %

4 6 %

4 4 %

4 2 % 4 2 %

4 2 %

4 2 % 42% 4 2 %

5 3 % 5 3 %

5 3 %

4 9 % 4 9 %

4 9 %

4 4 % 44% 4 4 %

4 6 % 46% 4 6 %

5 3 % 53% 5 3 %

4 9 % 49% 4 9 %

5 3 %

4 9 %

4 4 %

4 6 %

4 2 %

5 3 %

4 9 %

4 4 %

4 6 %

4 2 %

4 4 %

5 3 %

4 9 %

4 6 %

4 2 %

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

34 28 42 2 10 21 45 8 15 32 51 29 25 12 47 13 43 26 18 3 19 16 30 20 23 24 36 7 9 5 11 17 35 49 50 41 48 1 4 22 6 27 33

SURVIVAL  EBV

FAMILY  NUMBER

Estimated  Breeding  Value  for  Survival:  2011  Year  Class  Families

Heritability  =  0.38          þ  

A  group  of  cousins        þ  

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%34 28 42 2 10 21 45 8 15 32 51 29 25 12 47 13 43 26 18 3 19 16 30 20 23 24 36 7 9 5 11 17 35 49 50 41 48 1 4 22 6 27 33

SURV

IVAL

 EBV

FAMILY  NUMBER

Estimated  Breeding  Value  for  Survival:  2011  Year  Class  Families

A  group  of  cousins        ý  

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

34 28 42 2 10 21 45 8 15 32 51 29 25 12 47 13 43 26 18 3 19 16 30 20 23 24 36 7 9 5 11 17 35 49 50 41 48 1 4 22 6 27 33

SURVIVAL  EBV

FAMILY  NUMBER

Estimated  Breeding  Value  for  Survival:  2011  Year  Class  Families

FAMILY  2009003 FAMILY  2009025 FAMILY  2009027

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

INDIVIDUAL  SURVIVAL  EBV

Within  Family  Variation   (3  selected  families  only)  

Survival:  present  popula$on  34

%

32%

33%

20%

17%

14%

33%

32%

20%

43%

43%

43%

71%

37%

54%

69%

58%

53%

49%

62%

74%

58%

82%

78%

51%

56%

61%

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

AVERAGE  SURVIVAL  =  47%  Survival  in  2012  

Survival:  best  families  

SURVIVAL  IMPROVEMENT  =  47%  to  ~70%  Predicted  survival  in  2014  

60%

59%

60%

52%

50%

48%

60%

59%

52%

66%

66%

66%

83%

62%

72%

81%

75%

72%

69%

77%

84%

75%

89%

87%

71%

73%

77%

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

Survival:  best  individuals  and  best  families  

SURVIVAL  IMPROVEMENT  =  47%  to  ~80%  Predicted  survival  in  2014  

73%

73%

73%

68%

67%

65%

73%

73%

68%

77%

77%

77%

88%

75%

82%

88%

83%

81%

79%

85%

90%

83%

93%

91%

81%

82%

84%

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

Survival:  higher  disease  severity  

ASSUMED  AVERAGE  SURVIVAL  =  25%    

18%

17%

17%

11%

9% 7% 17%

17%

10%

23%

23%

23%

38%

19%

29%

37%

31%

28%

26%

33%

39%

31%

43%

42%

27%

30%

32%

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

Survival:  best  families  and  higher  disease  severity  

SURVIVAL  IMPROVEMENT  =  25%  to  40%  Predicted  survival  in  2014    

28%

27%

28%

17%

14%

12%

28%

27%

17%

36%

36%

37%

60%

31%

46%

58%

49%

45%

41%

53%

63%

50%

69%

67%

44%

47%

52%

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

Survival:  best  individuals  from  best  families  and  higher  disease  severity  

SURVIVAL  IMPROVEMENT  =  25%  to  50%  Predicted  survival  in  2014  

42%

40%

41%

33%

31%

29%

41%

41%

33%

48%

48%

48%

66%

44%

55%

65%

57%

54%

52%

60%

68%

58%

73%

71%

53%

56%

59%

Replicate 1 Replicate 2 Replicate 3Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

The  main  messages  

•  Gene$c  selec$on  will  improve  resistance  

•  But  it  is  most  likely  a  medium  term  solu$on  

•  S$ll  lots  to  learn  about  the  gene$c  inheritance  of  this  disease  

Acknowledgements  •  The  trial  site  was  provided  by  Drakes  Oysters  and  this  work  

would  not  have  been  possible  without  the  assistance  of  Bob  Drake,  Ted  Drake  and  Len  Drake  

•  This  study  was  done  as  part  of  the  Seafood  CRC  and  was  made  possible  with  funding  assistance  by  the  Seafood  CRC  

•  Richard  Whiengton  and  Ika  Paul-­‐Pont  (University  of  Sydney)  have  helped  out  by  generously  sharing  their  knowledge  and  experience