kristina simon bas kemp august 3, 2015 - · pdf filekristina simon aart lammers bas kemp...
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Effects of dextran sulfate sodium (DSS) on
(immune) development in broilers and layers
Kristina Simon
Aart Lammers
Bas Kemp
August 3, 2015
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Dextran sulfate sodium (DSS)
DSS via drinking water induces colitis
2
DSS affects intestinal barrier function
inflammatory response
DSS changes microbiota composition
effects on immune system
Ohkusa et al., 1995; Stevceva et al., 2001b; Faure et al., 2006; Lupp et al., 2007; Johansson et al., 2010
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Aim
DSS as a model for disruption of intestinal homeostasis in chickens?
3
Inflammatory response
accelerated immune development?
effects on immune response?
Differences between broilers & layers?
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Experimental Setup
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Early
Feeding
plain drinking water
plain drinking water
2.5 % DSS d 11 - d 18
2.5 % DSS d 11 - d 18
Challenge d 35 LPS/HuSA i.m.
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Measurements
Intestinal morphology
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Ileal cytokine and Ig mRNA expression levels
Antibody response after challenge
Performance
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Results
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Ileum control Ileum DSS
Broiler
Layer
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Colon control Colon DSS
Broiler
Layer
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Caecum control Caecum DSS
Broiler
Layer
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Intestinal length & performance
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DSS shorter colon & caecum d 18
Colon still shorter in DSS layers on d 35
0
500
1000
1500
2000
2500
3000
0 7 14 21 28 35 42 49
BW
(g
)
days post hatch
Broilers
DSS
control
**
***
0
100
200
300
400
500
600
0 7 14 21 28 35 42 49
days post hatch
Layers
DSS
control
***
***
***
***
*
Performance:
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Immune parameters
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Limited effects on ileal cytokine expression levels
Ileal Ig expression levels in DSS broilers
Antibody response towards LPS in DSS broilers
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Mortality
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Differences between broilers & layers: Mortality higher in layers (3% vs 11%) Even several days after cessation of DSS treatment
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Discussion
Mortality not expected no mortality in pilot experiment
Severity of colitis may depend on stress Less stress in pilot experiment
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Layers more affected by DSS & need more time to recover
More susceptible to DSS under stressful situation
Broilers have a higher cell turnover due to rapid growth
Intestinal damage more limited
Gué et al., 1997; Cameron & Perdue, 2005; Saito et al., 2005; Reber et al., 2006
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10 d 14 d 21 d
28 d 35 d 42 d
49 d
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Conclusion
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DSS may be a good model to investigate effects of disruption of intestinal homeostasis in chickens
BUT:
≠
More research needed on the appropriate dose
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Questions?