know thy self luxury genes - from forbes.com gift-hunting for a truly self-obsessed friend? consider...
TRANSCRIPT
![Page 1: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/1.jpg)
Know thy selfLuxury Genes - from Forbes.com
Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code
A firm in Cambridge, Mass. called Knome (pronounced "gnome") is offering the
service.
http://www.knome.com/Recent News
October 2008: Knome Commences Whole-Genome Sequencing Process For First Clients
![Page 2: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/2.jpg)
You, on a platter: A customer who pays $350,000 for Knome’s service receives a silver box containing an eight-gigabyte USB drive housing his or her genome sequence and specialized browser software. Credit: courtesy of Knome, Inc.
![Page 3: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/3.jpg)
PCR Amplifies Target DNA
in vitro• Replicating target DNA in vitro • Taq polymerase
• Temperature-resistant DNA polymerase
– Thermus aquaticus•Heat resistant•Best for <2 kb target
2581b Hill C9
![Page 4: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/4.jpg)
Fig. 9.16.4
PCR
Replicating target DNA in vitro2581b Hill C9
![Page 5: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/5.jpg)
Fig. 9.16.1
•Two primers bracket the target region to be amplified•Complementary to one strand•Anti-parallel
Amplify DNA PCR
2581b Hill C9
![Page 6: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/6.jpg)
Fig. 9.16.1
Amplify DNA PCR
The 3’ nucleotide is critical for specificity
2581b Hill C9
![Page 7: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/7.jpg)
Fig. 9.16.3
Amplify DNA PCR
Cycles of repeated replication
2581b Hill C9
![Page 8: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/8.jpg)
Fig. 9.16.2
Denature
AnnealExtend
Extend
2581b Hill C9
![Page 9: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/9.jpg)
PrimerArtefact
Lane M 1 2 3 4 N P 5 6 7 8 9 10
1.8 kb
What is the size of the PCR product?
Gel electrophoresis separates PCR amplicons on the basis of size.
2581b Hill C9
![Page 10: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/10.jpg)
• Exponential increase
• Need to know some sequence information
• Very sensitive
• Can amplify from limited starting material
• Ideal for 2kb target
PCR
Fig 8-8 2581b Hill C9
The PCR songhttp://www.youtube.com/watch?v=x5yPkxCLads&feature=related
![Page 11: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/11.jpg)
Fig. 9.17.1
DNA sequencing
2581b Hill C9
![Page 12: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/12.jpg)
Fig. 9.17.2
DNA sequencing
2581b Hill C9
![Page 13: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/13.jpg)
2581b Hill C9
![Page 14: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/14.jpg)
Fig. 9.17.2
DNA sequencing
ddTTP
2581b Hill C9
![Page 15: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/15.jpg)
Original Strand /Template strand
Nascent orNew Strand
Autoradiograph2581b Hill C9
![Page 16: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/16.jpg)
Fig. 9.17.3
2581b Hill C9
![Page 17: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/17.jpg)
Fig. 9.18.a
KH Genomics 10
![Page 18: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/18.jpg)
Fig. 10.20.d
Automated DNA Sequencing
KH Genomics 10
Gels are now replaced by capillaries filled with polymers
![Page 19: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/19.jpg)
Sequencing Chromatogram
KH Genomics 10
5’
3’Nascent or Newly synthesized strand
Close to the sequencing primer
Far from the sequencing primer
![Page 20: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/20.jpg)
Page 349
Heterozygote Detection
Maternal templatesequence A
Paternal templatesequence C
SNPSingle nucleotide polymorphism
KH Genomics 10
![Page 21: Know thy self Luxury Genes - from Forbes.com Gift-hunting for a truly self-obsessed friend? Consider buying him/her a reading of his/her entire DNA code](https://reader030.vdocuments.us/reader030/viewer/2022032702/56649f425503460f94c61c72/html5/thumbnails/21.jpg)
Page 349
Heterozygote Detection
KH Genomics 10
What would a chromatogram look like if the maternal copy had a deletion of one nucleotide?
A typical DNA sequencing read is 1000 nucleotides