Kingdom of Saudi

Arabia

King Saud University

College of Applied Medical

Sciences

Biomedical Technology

Department

By:

Badr AlShamary & Mohannad AbuljadayelSupervised By:

Mr. Mohammed Nisar

Polymerase Chain Reactionmolecular biological method for amplifying

DNA without using a living organism. PCR is commonly used in:

Identification of genetic fingerprints.Diagnosis of infectious diseases.Diagnosis of virus diseases.

A

T

G

C A

T C

G

The four bases found in DNA are Adenine (A), Cytosine (C), Guanine (G) and Thymine (T).

Thermocycler

Temperature Graph

Sample Contents

DNA original Sample

•DNA original Sample•Primers•DNA Polymerase•Buffer Solution•Nucleotides(A,T,C and G)

PrimersDNA Polymeras

e

Buffer SolutionNucleotid

es

PCR ProcessStep1: Denaturation

PCR ProcessStep2: Annealing

PCR ProcessStep3: Extension

PCR ProcessDoubling DNA each Cycle (Cycle:2)

PCR Process (Cycle: 3)

Number of copies=3

2 = 8

Virus Diagnosis By PCR

Human DNA

Virus DNA

Human DNA

Virus Diagnosis By PCR

Virus DNA

Human DNA

Virus Diagnosis By PCR

Virus DNA

Thank You