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Kevin Flowers 1129 Haral Place, Cherry Hill, NJ 08034 | 856-816-7428 | [email protected] Objective · To find a career opportunity that will challenge me to utilize the skills I have developed through the course of my career and grow within a company that is dedicated to its future. Education BACHELORS OF ARTS |MAY 2008 | RUTGERS CAMDEN · Major: Biology Experience RESEARCH ASSOCIATE – MOLECULAR ANALYSIS | DUPONT PIONEER | NOVEMBER 2011 - PRESENT · Isolate DNA using Hot Shot and Phenol/ Chloroform methods · Identify single copy and homologous recombination events using qPCR. · Analyze and record customer results using ABI software and PCR-GT. · Manage and maintain Vector DNA Library using Excel and Preps Inventory software. · Use restriction enzyme digest and electrophoresis to obtain and purify desired fragment DNA. · Grow and maintain Agro DNA cell cultures and do DNA purification to submit for Vector Quality Control (VQC) · Submitted and Analyzed samples for Next Gen sequencing · Trained and helped team members on new applications and machines in the lab. · Worked with team members to manage our time to meet lab deadlines. · Worked in an ISO9001 lab. · Knowledge of PCR, aseptic techniques, gel purification, Prism, PCR-GT, VectorNTI software. · Knowledge of Viia 7, ABI 7900, ABI 7500 qPCR machines and software, Eppendorf epMotion liquid handler. RESEARCH ASSISTANT – GENETIC DISCOVERY | DUPONT PIONEER – CONTRACTOR, YOH INC | APRIL 2010 – NOVEMBER 2011 · Maintained, managed, and recorded plant assays being run in the lab and growth chambers. · Performed weekly laboratory duties including disposal of biohazard waste and safety shower and eyewash checks · Inoculated bacterial cultures for transformation and plasmid preparation. · Performed DNA extraction, purification, cloning and transformation.

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Page 1: Kevin Flowers Resume (2)

‍‍Kevin‍Flowers1129‍Haral‍Place,‍Cherry‍Hill,‍NJ‍08034 | 856-816-7428 | [email protected]

Objective

· To‍find‍a‍career‍opportunity‍that‍will‍challenge‍me‍to‍utilize‍the‍skills‍I‍have‍developed‍through‍the‍course‍of‍my‍career‍and‍grow‍within‍a‍company‍that‍is‍dedicated‍to‍its‍future.

EducationBACHELORS OF ARTS |MAY 2008 | RUTGERS CAMDEN

· Major:‍Biology

ExperienceRESEARCH ASSOCIATE – MOLECULAR ANALYSIS | DUPONT PIONEER | NOVEMBER 2011 - PRESENT

· Isolate‍DNA‍using‍Hot‍Shot‍and‍Phenol/‍Chloroform‍methods

· Identify‍single‍copy‍and‍homologous‍recombination‍events‍using‍qPCR.

· Analyze‍and‍record‍customer‍results‍using‍ABI‍software‍and‍PCR-GT.‍

· Manage‍and‍maintain‍Vector‍DNA‍Library‍using‍Excel‍and‍Preps‍Inventory‍software.‍

· Use‍restriction‍enzyme‍digest‍and‍electrophoresis‍to‍obtain‍and‍purify‍desired‍fragment‍DNA.

· Grow‍and‍maintain‍Agro‍DNA‍cell‍cultures‍and‍do‍DNA‍purification‍to‍submit‍for‍Vector‍Quality‍Control‍(VQC)

· Submitted‍and‍Analyzed‍samples‍for‍Next‍Gen‍sequencing‍

· Trained‍and‍helped‍team‍members‍on‍new‍applications‍and‍machines‍in‍the‍lab.‍

· Worked‍with‍team‍members‍to‍manage‍our‍time‍to‍meet‍lab‍deadlines.

· Worked‍in‍an‍ISO9001‍lab.‍‍‍

· Knowledge‍of‍PCR,‍aseptic‍techniques,‍gel‍purification,‍Prism,‍PCR-GT,‍VectorNTI‍software.

· Knowledge‍of‍Viia‍7,‍ABI‍7900,‍ABI‍7500‍qPCR‍machines‍and‍software,‍Eppendorf‍epMotion‍liquid‍handler.

RESEARCH ASSISTANT – GENETIC DISCOVERY | DUPONT PIONEER – CONTRACTOR, YOH INC | APRIL 2010 – NOVEMBER 2011

· Maintained,‍managed,‍and‍recorded‍plant‍assays‍being‍run‍in‍the‍lab‍and‍growth‍chambers.‍

· ‍Performed‍weekly‍laboratory‍duties‍including‍disposal‍of‍biohazard‍waste‍and‍safety‍shower‍and‍eyewash‍checks

· ‍Inoculated‍bacterial‍cultures‍for‍transformation‍and‍plasmid‍preparation.

· Performed‍DNA‍extraction,‍purification,‍cloning‍and‍transformation.

· Utilized‍PCR‍techniques‍and‍gel‍electrophoresis.

· Use‍Molecular‍techniques‍to‍obtain‍DNA‍to‍send‍to‍sequencing‍and‍reviewed‍results

· Operated‍large‍particle‍flow‍cytometer.

PLANT PATHOLOGY INTERN | RUTGERS UNIVERSITY | MAY 2008 – DECEMBER 2009

· Prepare‍and‍analyzed‍roots‍samples‍looking‍for‍Mycorrhizae.

· Used‍cell‍culture‍techniques‍to‍Isolate‍Fungi‍types.

· Tested‍and‍recorded‍effects‍of‍Mycorrhizae‍on‍cranberry‍plants.

Page 2: Kevin Flowers Resume (2)

· Isolated‍DNA‍and‍RNA‍from‍various‍samples.

· Made‍various‍types‍of‍cell‍culture‍media.

· Utilized‍PCR,‍gel‍electrophoresis‍and‍tissue‍grinder.‍

Page‍2


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