k d = 23 µm

3

0 1 2 3 4 5 6 7 8 -4 -2 0 -1.5 -1.2 -0.9 -0.6 -0.3 0.0 -10 0 10 20 30 40 50 60 70 80 Tim e (m in) MolarRatio K D = 23 µM H3K4me1 0 5 10 15 20 25 -2 -1 0 -1.8 -1.5 -1.2 -0.9 -0.6 -0.3 0.0 -10 0 10 20 30 40 50 60 70 80 Tim e (m in) MolarRatio H3K9me1 K D = 43 µM 0 1 2 3 4 5 6 7 -4 -2 0 -1.2 -0.9 -0.6 -0.3 0.0 -10 0 10 20 30 40 50 60 70 80 Time (min) MolarRatio K D = 9 µM H3K9me2 0 1 2 3 4 5 -1.0 -0.8 -0.6 -0.4 -0.2 0.0 0.2 -0.8 -0.6 -0.4 -0.2 0.0 -10 0 10 20 30 40 50 60 70 80 Time (min) MolarRatio K D = 56 µM 0 1 2 3 4 5 -1.2 -1.0 -0.8 -0.6 -0.4 -0.2 0.0 0.2 -1.0 -0.8 -0.6 -0.4 -0.2 0.0 -10 0 10 20 30 40 50 60 70 80 Time (min) MolarRatio K D = 38 µM H3K27me1 H3K27me2 Supplementary Figure 3a characterization of L3MBTL2 4MBT binding with different histone pept

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ITC characterization of L3MBTL2 4MBT binding with different histone peptides. H3K4me1. H3K9me1. H3K27me1. H3K9me2. H3K27me2. K D = 23 µM. K D = 43 µM. K D = 56 µM. K D = 9 µM. K D = 38 µM. Supplementary Figure 3a. Monomethylated Lysine interaction with L3MBTL2. - PowerPoint PPT Presentation

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Page 1: K D = 23 µM

0 1 2 3 4 5 6 7 8

-4

-2

0

-1.5

-1.2

-0.9

-0.6

-0.3

0.0

-10 0 10 20 30 40 50 60 70 80

Time (min)

µcal/sec

Molar Ratio

kcal/mole of injectantKD = 23 µM

H3K4me1

0 5 10 15 20 25-2

-1

0

-1.8

-1.5

-1.2

-0.9

-0.6

-0.3

0.0

-10 0 10 20 30 40 50 60 70 80

Time (min)

Molar Ratio

H3K9me1

KD = 43 µM

0 1 2 3 4 5 6 7

-4

-2

0

-1.2

-0.9

-0.6

-0.3

0.0

-10 0 10 20 30 40 50 60 70 80

Time (min)

Molar Ratio

KD = 9 µM

H3K9me2

0 1 2 3 4 5-1.0

-0.8

-0.6

-0.4

-0.2

0.0

0.2-0.8

-0.6

-0.4

-0.2

0.0

-10 0 10 20 30 40 50 60 70 80

Time (min)

Molar Ratio

KD = 56 µM

0 1 2 3 4 5

-1.2

-1.0

-0.8

-0.6

-0.4

-0.2

0.0

0.2-1.0

-0.8

-0.6

-0.4

-0.2

0.0

-10 0 10 20 30 40 50 60 70 80

Time (min)

Molar Ratio

KD = 38 µM

H3K27me1 H3K27me2

Supplementary Figure 3a

ITC characterization of L3MBTL2 4MBT binding with different histone peptides

Page 2: K D = 23 µM

The binding of monomethylated lysine to L3MBTl2 is very weak (~4000 uM)

Monomethylated Lysine interaction with L3MBTL2

Supplementary Figure 3b

0 2 4 6 8 10-0.30

-0.24

-0.18

-0.12

-0.06

-1.0

-0.8

-0.6

-0.4

-0.2

0.0

0 10 20 30 40 50 60 70 80

Time (min)

µcal/sec

Molar Ratio

kcal/mole of injectant

Page 3: K D = 23 µM

KD (µM) N ∆H

(cal . mol-1)

∆S

(cal . mol-1)

∆G

(cal . mol-1 . K-1)

H3K4me

H3K9me

H4K20me

H4K27me

H3K9me2

H4K20me2

H4K27me2

23

43

14

56

9

11

38

1.1

1.1

0.9

1.2

1.0

1.2

0.9

-6921

-5047

-15000

-1309

-5083

-3585

-1805

-2

3

-33

15

6

11

14

-6330

-5940

-5170

-5780

-6870

-6710

-5980

Thermodynamic parameters of the interaction of L3MBTL2 with histone peptides

Supplementary Figure 3c

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