just imagine - science...0 0.1 0.2 1.0 t4 dna ligase (µl) 0 10 20 30 60 time (min) ligation...
TRANSCRIPT
Just imagine...
sigma-aldrich.com
Imagine a tool that gives you the power
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Sigma and Sigma-Aldrich are registered trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P.
The red cube symbol is a trademark belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P.
Ingenuity is a registered trademark belonging to Ingenuity Systems.
It’s the all-new YFG. And it’s free.Think forward, work smarter with
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Go ahead, ask the big questions.Our miRNA and noncoding RNA profiling portfolio makes it possible.
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2009 © Invitrogen Corporation. All rights reserved.
GE Healthcare
Coordinate your application focus.Achieve greater operational efficiency.GE Healthcare is focused on helping you develop efficient biopharmaceutical
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GE Healthcare Bio-Sciences AB, a General Electric CompanyBjörkgatan 30, 751 84 Uppsala, Sweden© 2008 General Electric Company – All rights reserved.
GE04-09. First printetd 09/2008.
IN A WORD, TRUST.
T4 DNA LigaseNew England Biolabs is dedicated to providing our customers with guaranteed enzyme performance. Our recombinant T4 DNA Ligase
is the most extensively used ligase for cloning experiments and other applications, including sample preparation and next generation
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Flexibility - Active at room temperature or
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Robustness - Active in a variety of
reaction buffers
CLONING & MAPPINGDNA AMPLIFICATION
& PCRRNA ANALYSIS
PROTEIN EXPRESSION
& ANALYSIS
GENE EXPRESSION
& CELLULAR ANALYSIS
Ligation of blunt-ended HaeIII
fragments of Lambda DNA using
various amounts of T4 DNA Ligase
(400,000 cohesive end units/ml) in
a 20 µl reaction volume. Reactions
were incubated for 30 minutes
at 16°C.
0 0.1 0.2 1.0T4 DNA Ligase (µl)
0 10 20 30 60Time (min)
Ligation of HindIII fragments
(4-base overhang) of Lambda
DNA using 1 cohesive end
unit (1µl of 1:400 dilution)
of T4 DNA Ligase. Reactions
were incubated at 25°C.
T4 DNA Ligase* r
Regular Concentration ................ M0202S/L
For standard cloning reactions
High Concentration .................. M0202T/M
For large or difficult constructs
Quick Ligation™ Kit* r ............ M2200S/L
For ligation of cohesive or blunt-end DNA
fragments in 5 minutes at room temperature
r = Recombinant
*NEB ligase products are BSA-free
For more information regarding customization and
OEM opportunities, please contact [email protected]
New England Biolabs, Inc. is an ISO 9001 certified company
This annual international research prize recognizes
accomplishments in neurobiology research based on
methods of molecular and cell biology. The winner and
finalists are selected by a committee of independent
scientists, chaired by the Editor-in-Chief of Science.
Past winners include postdoctoral scholars and
assistant professors.
To be eligible, you must be 35 years of age or younger.
If you´re selected as this year´s winner, you will receive
$25,000, have your work published in the prestigious
journal Science and be invited to visit Eppendorf in
Hamburg, Germany.
Deadline for entries
June 15, 2009
2008 Winner
Mauro Costa-Mattioli, Ph.D.
Department of NeuroscienceBaylor College of Medicine,
Houston, Texas
Eppendorf & Science Prize for
Neurobiology
For more information
www.eppendorf.com/prize
Get recognized!
$25,000Prize
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Express proteins in hours instead of days. Referenced
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For Research Use Only. Not for use in diagnostic procedures. Practice of the patented 5’ Nuclease Process requires a license from Applied Biosystems. The purchase of TaqMan® SNP Genotyping Assays includes an immunity fromsuit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research when used with the separate purchase of an Authorized 5’ Nuclease Core Kit. No other patent rights areconveyed expressly, by implication, or by estoppel. For further information on purchasing licenses contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. © 2009 AppliedBiosystems. All rights reserved. Applied Biosystems and AB (Design) are registered trademarks of Applied Biosystems or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademark of Roche MolecularSystems, Inc. OpenArray is a registered trademark of BioTrove, Inc.
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LIFE SCIENCE TECHNOLOGIESAAAS/Science Business Office
Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizationsare featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of anyproducts or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.
Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.
113www.sciencemag.org/products SCIENCE VOL 324 3 APRIL 2009
Tissue Culture PlatesA new product line features tissue culture plates coated with a selection
of matrices that enhance cell growth and differentiation for stem
cell, cancer, cell signaling, and other research applications. Millicoat
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Millipore
For information 978-762-5170
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Automated DispensingFill-It is a new product for rapidly dispensing cell suspensions and
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The Automation Partnership
For information +44-(0)-1252-37300
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Parallel Research SystemThe Synthesis 1 can be configured for parallel solid and liquid phase
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Heidolph UK
For information +44-1799-513320
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High Throughput HPLC SystemThe ExpressHT-Ultra HPLC (high performance liquid chromatography)
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drugmetabolism.With cycle timesas short as60 seconds, theExpressHT-
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Featuring columns with diameters of 1 mm or smaller, the system
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Eksigent
For information 925-560-2628
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Molecular Modeling SoftwareFieldAlign and FieldTemplater are designed for molecular modelers
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feature proven Field Point technology to describe molecules of interest
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to a three-dimensional field template created from an active molecule.
The molecules the user is considering synthesizing can be entered in
two dimensions. FieldTemplater finds the three-dimensional bioactive
conformation from known active compounds. Three active compounds
are all that is needed to get started.
CambridgeSoft
For information 800-315-7300
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Electrophoresis SystemsTwonewgel systemsare suited for high throughput electrophoresis of polymerase chain reaction (PCR)
products from 96-well microtiter plates. The compact CSL multiSUB Midi 96 system is designed to be
used in conjunction with samples from 96-well plates to allow direct loading and easy identification
of samples. The run length between samples is 17mm. The multiSUB MIDI96 stretch has a run length
of 34 mm for extended separations. With an average run-time of just 15 to 30 minutes, the Midi 96
version allows rapid identification of samples. The eight-channel pipettes facilitate sample loading.
Two comb types are available, one with eight wells plus an additional lane for DNA markers and one
with eight wells plus two lanes for markers. Custom-made combs are also available. The buffer volume
of the Midi 96 is just 300ml, which can be reduced to 200ml by using buffer saver blocks.
Cleaver Scientific
For information +44-(0)-1788-565-300
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New Products
Dr. Nubia MuñozVisiting ScientistCatalan Institute of OncologyBarcelona, Spain
�e 2009 Canada Gairdner International Awardees are:
�e Inaugural Canada Gairdner Global Health Awardee is:
“for her epidemiological studiesthat defined the essential role of thehuman papilloma virus in theetiology of cervical cancer on a globallevel which led to the developmentof successful prophylactic vaccines”
SEVEN OF THE WORLD’S LEADINGSCIENTISTS ARE RECIPIENTS OF THE2009 CANADA GAIRDNER AWARDS
50 years ago, JamesGairdner createdThe Gairdner Awardsto recognize thebreakthroughs of theworld’s leading medicalscientists and to bringthe workings of ‘highscience’ to the public.The Gairdners arenow Canada’s topinternational prize andone of the most esteemedawards in the world ofscience.
The importance of theGairdners to Canada’sfuture as a world leaderin scientific research wasunderscored when theGovernment of Canadalast year announced anendowment to increaseits awards to $100,000each and to create theworld’s first individualaward for Global Health.Starting this year, theywill benamed theCanadaGairdner InternationalAwards and the CanadaGairdner Global HealthAward.
Supported nationally by:
Gairdner WightmanAwardDr. David Sackett. MD, ScDProfessor EmeritusMcMaster UniversityHamilton, OntarioCanada
“for his leadership in the fields ofclinical epidemiology and evidence-based medicine, which have hadmajor impacts internationally inapplied clinical research and inthe practice of medicine”
Dr. Shinya YamanakaProfessor, Department of Stem Cell BiologyInstitute for Frontier Medical SciencesDirector, Centre for iPS Cell Research andApplicationsInstitute for Integrated Cell-Material SciencesKyoto UniversityJapan
“for his demonstration that thekey transcription factors whichspecify pluripotency may becomereprogrammed somatic cells topluripotent stem cells”
Dr. Kazutoshi MoriProfessor, Department of BiophysicsGraduate School of ScienceKyoto UniversityJapan
Dr. Peter WalterHoward Hughes Medical InstituteDepartment of Biochemistry and BiophysicsUniversity of California, San FransicsoSan Francisco, CA
“for their dissection and elucidationof a key pathway in the unfoldedprotein response which regulatesprotein folding in the cell”
Dr. Richard LosickMaria Moors Cabot Professor of BiologyHoward Hughes Medical Institute ProfessorFaculty of Arts and SciencesHarvard UniversityCambridge, MA
Dr. Lucy ShapiroD.K. Ludwig ProfessorDepartment of Developmental BiologyDirector, Beckman Center for Molecular andGenetic MedicineStanford University School of MedicineStanford, CA
“for their discovery of mechanismsthat define cell polarity andasymmetric cell division, processeskey in cell differentiation and inthe generation of cell diversity”
www.gairdner.org
R&D Systems Tools for Cell Biology Research™
USA & Canada R&D Systems, Inc. Tel: (800) 343-7475 [email protected]
Europe R&D Systems Europe, Ltd. Tel: +44 (0)1235 529449 [email protected]
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