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Syllabus)

November 13hours(IV)1110-1335

The mitochondrion, respiratory chain, electron transport system, production of ATP, chemiosmotic hypothesis.The cytoskeleton (microtubules, microfilaments, intermediate filaments), cell motility, movement of organelles, cellular motors.

Practical courses & seminars

Lecturers

Prof. Dr. Miroslav Cervinka, M.D., Ph.D. Prof. Dr. Emil Rudolf, Ph.D. Dr. Vera Kralova, Ph.D.

Practical courses

7. 10.1. Microscopic techniques I.

1.1 General terms and conditions of the biology course 1.2 Introduction to light microscopy 1.3 Permanent mount of cells cultivated in vitro, size estimation 1.4 Vital staining 14. 10. 2. Microscopic techniques II. 2.1 Phase contrast microscopy 2.2 Fluorescence microscopy 2.3 Native and permanent mount of animal cells (buccal smear) 21. 10. 3. Cellular membranes - osmotic phenomena 3.1 PEGstimulatedfusion 3.2 Osmoticchangesinerythrocytesafterthetreatmentwithanisotropicsolutions 3.3 Plasmolysisinplantcell(Elodeadensa) 4. 11. 4. Cell motility 4.1 Cytoskeleton-thestructureandfunction 4.2 Cyclosisinplantcells(Elodeadensa) 4.3 Amoeboidmovement 4.4 Flagellarmovement

11. 11.5. Cell cultures in biomedicine

5.1 Modelinginbiomedicine(3Rprinciple,in vitro models) 5.2 Cultivation of cells in vitro 5.3 Introductiontothetissueculturelaboratory 5.4 Practicaldemonstrationoflaboratorytechniques 18. 11. 6. Information sources and their relevance in biomedicine (seminar) 6.1 Scientific information in biomedicine - the structure of scientific article 6.2 Electronic bibliographic databases (MedLine, EBSCO, Proquest) - a mini review 6.3 In vitro tests used for cytotoxicity assays - dental applications 25. 11. 7. Cell death - scientific methodology 7.1 Cultured cells in suspension, vital staining 7.2 Influence of UV light on survival of Paramecium 7.3 Photodynamic effect 2. 12. 8. Cell cycle and cell division

8.1 Mitosis in permanent mounts of plant and animal cells 8.2 Mitosis in cells of cell cultures 8.3 Determination of the mitotic index and particular phases of mitosis 9. 12. 9. Advances in biosciences (seminar) 9.1 Student presentations (biodentistry oriented) 16. 12. 10. CREDIT 10.1 Final test

Rohini Selvarajah,2003/2004

Guaranteed by: Department of Medical Biology and Genetics (15-120)

Faculty: Faculty of Medicine in Hradec Králové

Requirements to the exam

Requirements for credit

a) Attendance + active participation

Attendance at practical classes is mandatory. Each student is allowed one absence (medically excused) from one practical class.

Practicals begin at 11.10 a.m. and students are required to be in their seat. If the student is not present at that time, he will be marked absent.

Students must be prepared for the class.

b) Report, karyotype, presentation, manual

Studentsareobligedtohandintheirownkaryotypealongwithareferencephotograph.Thedeadlineis

Students are also obliged to preparean abstract of their oral presentation and submit this abstract via email for checkout.The topics will be specified at the beginning of the semester. The deadline is December 6th 2003 . In case of the inappropriate presentation topic, we reserve the right to reject it and ask for the refinement or topic change. The full text version of presented topics should be submitted by e-mail before December 20th 2003.

Manualpresentedduringcreditsessionmusthaveanappropriateexternalform;i.e.beclean,intactasithasstatusofofficialdocument.

c) Tests

There is one final test which must be passed. Passing grade is 75 %. In case of the test failure, the student has two more chances to pass written make-up tests.

d) CreditThe credit in this term consists of:

Final test with minimal scoring grade of 75 %

Completedmanualincludingdrawingsandanswerstoquestions (in case of any manual damage or loss, the student has to acquire a new one from the department against financial compensation).

Preparedkaryotype Successfully presented topic (evaluation of presentation will include the topic itself as well as

the quality of presentation)

Appointmentsforwintercreditarescheduledtowinter examination period (January 20st 2004 - February 16th 2004), summer examination period (June 2rd 2004 - June 29 th 2004) andduring September (1. - 26. 9. 2004),each week on Thursdays from 2 to 3pm only.

Generally, in case of failure in credit (final test), the next possible chance is after one week!!!!

The students who seek the exemption from practical classes in medical biology and genetics have to submit their request before the start of practical classes. Their request must be accompanied by a detailed syllabus of biology course they had completed which will be evaluated by the head of the department of medical biology and genetics.

e) Examination

Before signing for the final examination each student must have two credits, one credit in the summer term of the first year and the second one in the winter term of the second year.

Cheating during any form of biology exam (credit test and oral examination) including preparatory period is not tolerated! Use of any electronic devices (cell phones, tablets, notebooks, phablets etc.), unauthorized materials or communication with other students in these instances is strictly prohibited! After selecting final exam questions it is not allowed to leave the room and when necessary an escort will be provided. In case of failure to comply with these rules, student will be classified "failed".

Syllabus)

Lecturers

Prof. Dr. Miroslav Cervinka, M.D., Ph.D. Prof. Dr. Emil Rudolf, Ph.D.

October 13 hours (III) 800-1025

The mitochondrion, mitochondrial DNA.The cytoskeleton (microtubules, microfilaments, intermediate filaments), cell motility, movement of organels, cellular motors.

October 83 hours (II) 800-1025

Cell growth and cell division, cell cycle, mitosis.Cell death, apoptosis.Gene, genetic code.


Mutations, reparation of damaged DNA, screenings for mutagens.Chromosomes, human karyotype. Structural and numerical aberrations of autosomes.


Sexual reproduction, meiosis, abnormalities of gonosomesX-linked genes, X chromosome inactivation.


Genetic manipulations - basic methods and principles.

November 52 hours (II) 850-1025

DNA sequencing, genomics.Recombination of DNA, PCR, cDNA.


DNA diagnostics. Production of recombinant molecules.


Gene therapy.


Genetics of multicellular organisms, Mendel’s law, Morgan’s law, pedigree analysis.

December 32 hours (II) 850-1025

Twins in genetic research, cytogenetics, prenatal diagnosis.


Population genetics - Hardy-Weinberg law.


Metabolic disorders.

Practical courses

Lecturers

Prof. Dr. Emil Rudolf, Ph.D. Dr. Vera Kralova, Ph.D.

8., 10.10.1. The cell cycle and cell division

1.1 Mitosis in permanent mounts of plant and animal cells 1.2 Mitosis in cells of cell cultures 1.3 Determination of the mitotic index and particular phases of mitosis 15., 17.10. 2. A Karyotyping using human cells (1st subgroup) 2. B The summer term DNA samples- electrophoresis (2nd subgroup) 2. B1 Blotting, DNA hybridization 29., 31. 10.3. A The summer term DNA samples- electrophoresis (1st subgroup) 3. A1 Blotting, DNA hybridization 3. B Karyotyping using human cells (2nd subgroup) 22.,24.10. 4. Eukaryotic chromosomes and meiosis 4.1 Polytene chromosomes of Drosophila melanogaster 4.B.12Meiosis in cells of locust 5., 7.11. 5. Cytogenetic tests and their use in clinical genetics 5.1 Clinicalindicationsforkaryotypeanalysis 5.2 Chromosomeidentificationandclassification(banding,SCE,FISHandothertechniques) 12.,14.11. 6. Sex chromatin 6.1 Sexchromatin-classificationandlocalization 6.2 Structuralandnumericalaberrationsofsexchromatin 6.3 Sexchromatininbuccalcells 6.4 Sexchromatininleukocytesmear 6.5 Evaluationofthepreparedkaryotypes 19., 21.11. 7. Mendelian genetics-pedigrees 7.1 Pedigree analysis, symbols and rules applied 7.2 Autosomal and X-linked inheritance 7.3 Selected clinical cases - workshop I

26., 28.11. 8. Molecular biology techniques in human genetics Biological typing in man 8.1 Genetics of ABO, MNSs and Rh blood groups 8.2 DNA diagnostics 8.3 Selected clinical cases - workshop II 3., 5. 12. 9. Tumor biology I. 9.1 Tumor classification (benign and malignant) 9.2 Basic histopathology of the selected tumors - demonstration 9.3 Normal and tumor cell in vitro and in vivo 9.4 Karyotypes of normal and tumor cells 10., 12.12. 10. Tumor biology II. 10.1 Cell malignant transformation - mutagenesis and carcinogenesis 10.2 Protooncogenes, oncogenes and TSGs 10.3 Defects in DNA repair genes 10.4 Familial cancer syndromes and other heritable malignancies 17., 19. 12.11. Population genetics 11.1 Introductory concepts - Hardy-Weinberg law 11.2 Practical applications of population studies 11.3 Students presentation I 7., 9.1. 20 12. Advances in molecular biology in 2013 12.1 Students presentation II 12.2 Discussion 14., 16.1. 13. CREDIT 13.1 Final revision test

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