john a.g. briggs retrovirus structure and assembly
DESCRIPTION
John A.G. Briggs Retrovirus structure and assembly. The structural genomics of HIV. Gag. Immature virion. Immature HIV. p17. p24. p7. p2. p1. Gag cleavage – can be inhibited. maturation. MMTV. Immature virion. HIV. 2 pathways of Retrovirus formation. Immature virion. - PowerPoint PPT PresentationTRANSCRIPT
John A.G. Briggs
Retrovirus structure and assembly
The structural genomics of HIV
Gag
Immature virion
Immature HIV
Gag cleavage – can be inhibited
p2 p1p17 p24 p7
maturation
MMTV
HIV
2 pathways of Retrovirus formation.
Immature virion
Immature virion
Internal scaffold domainCytoplasmic retention motif
MMTV
HIV
Envelopment?
Immature virion
cytosol
cytosol
maturation
Finch/Sundquist lab
Core formed from ~1200 capsid proteins
Arranged in ~96Å hexagonal lattice
maturation
What is the structure of the lattice here?
In-vitro assembledImmature HIV-1
If it is made from 80A hexagons, with six proteins per hexagon then 3400 ± 200 gag proteins
Mean mass: 165 ± 29 MDa
3800 ± 700 gag proteins
Scanning Transmission Electron Microscopy
145nm immature virion formed from ~5000 Gag proteins
Arranged in ~80 Å hexagonal lattice
Immature virion
Tighter hexagonal lattice with 8 nm unit cell.
~5000 gag proteins form a complete shell for a 145 nm virus
Mature virion
Looser hexagonal lattice with 9.6 nm unit cell.
~1200 CA proteins form the conical core, leaving a large number elsewhere
VirologyUniversity of HeidelbergUniversity of OxfordCornell University
Hans-Georg KraüsslichBarbara MüllerMarko LampeBärbel GlassMarc JohnsonVolker VogtQuentin Sattentau
Electron MicroscopyUniversity of OxfordMax-Planck Institute for Biochemistry
Stephen FullerKay GrünewaldFriedrich FörsterThomas WilkGiulia Zanetti