jeffrey chou ee235
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Nanoimprint Lithography Based Fabrication of Shape-Specific Enzymatically -Triggered Smart N anoparticles. Jeffrey Chou EE235. Papers Reviewed. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Jeffrey Chou EE235](https://reader036.vdocuments.us/reader036/viewer/2022062323/5681629e550346895dd3146e/html5/thumbnails/1.jpg)
Nanoimprint Lithography Based Fabrication of Shape-Specific Enzymatically-Triggered Smart Nanoparticles
Jeffrey ChouEE235
![Page 2: Jeffrey Chou EE235](https://reader036.vdocuments.us/reader036/viewer/2022062323/5681629e550346895dd3146e/html5/thumbnails/2.jpg)
Papers Reviewed
[1] Luz Cristal Glangchai, Mary Caldorera-Moore, Li Shi, Krishnendu Roy, Nanoimprint lithography based fabrication of shape-specific, enzymatically-triggered smart nanoparticles, Journal of Controlled ReleaseVolume 125, Issue 3, , 11 February 2008, Pages 263-272.
[2] J.A. Champion, Y.K. Katare and S. Mitragotri, Particle shape: a new design parameter for micro-and nanoscale drug delivery carriers, J. Control. Release 121 (1–2) (2007), pp. 3–9.
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Basic Idea
Enzyme Release
Drug Encapsulation
PEGDA Insert to Cells
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Nanoimprint Fabrication
1)PEGDA Applied to silicon
2)Quartz template pressed onto PEGDA
3)Remove template4)Oxygen etch to
remove residual layer5)Particles harvested by
water buffer.
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Shape Variability for Delivery
-Shape form factors, aspect ratios, or edges affect particle transport.
- Eg: Cell membrane interaction is heavily dependent on shape of particle.
50nm -> 400nm particles
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Shape Dependence
-With nanoimprint, a wide variety of shapes and depth can be made for custom drug delivery.
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Drug Encapsulation
-Fluorescently labeled antibodies were trapped within 400nm nanocarriers.- After releasing, particles remained intact by retaining its fluorescence.- Shows that encapsulation does not degrade drug
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Enzyme Based Release-PEGDA is mixed with an enzymatically degradable peptide GFLG- GFLG Sensitive to Cathepsin B enzyme
- Which is present in lung, ovarian, and colorectal tumor cells.
- Other peptides can be used for other diseases
- Eg: Breast cancer cells have high concentrations o f Cathepsin D enzyme
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Enzyme Release
Cathepsin B based biodegradable encapsulation-(a) No Cathepsin B- (b) 30min. In Cathepsin B- (c) 12h in Cathepsin B- (d) 48h in Cathepsin B
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Basic Idea
Enzyme Release
Drug Encapsulation
PEGDA Insert to Cells
![Page 11: Jeffrey Chou EE235](https://reader036.vdocuments.us/reader036/viewer/2022062323/5681629e550346895dd3146e/html5/thumbnails/11.jpg)
ConclusionDemonstrated the potential for enzyme
based nano drug delivery method for cells.PEGDA and drugs can be easily mixed.Nanoimprint allows for small particles
(~50nm) to be internalized by cells.Enzyme based release allows for “smart”
drug delivery.