jay t. groves · division director's fellow in the physical biosciences division at lawrence...

Jay T. Groves received his B.S. degree in

Physics and Chemistry from Tufts

University, and then went on to complete

his Ph.D. in Biophysics with Professors

Steven Boxer and Harden McConnell at

Stanford University. He then spent a year

as a visiting scholar at Academia Sinica in

Taipei, Taiwan before becoming the

Division Director's Fellow in the Physical

Biosciences Division at Lawrence Berkeley

National Laboratory. In 2001 he joined the

Chemistry Department at UC Berkeley as

an Assistant Professor. He was promoted to

Over the last twenty years, there has been

a staggering rate of development in optical

imaging technology. This has been largely

driven by the consumer electronics

industry, but the benefits to basic science

have been tremendous. Single molecule

imaging is now routine, and was even

recognized by awarding the Nobel Prize in

Chemistry for 2014 to W. E. Moerner, Eric

Betzig, and Stefan Hell. Single molecule

imaging continues to expose the molecular

level inner workings of living cells at

DAY 01

Jay T. GROVES

Professor Department of ChemistryUC Berkeley, USA

Molecular Imaging: From single molecule studies in living cells to handheld diagnostics

Biography

Associate Professor in 2007 and Professor

in 2010. In 2008 Professor Groves was

appointed as a Howard Hughes Medical

Institute Investigator. He has received the

Burroughs Wellcome Career Award in the

Biomedical Sciences (2000), the Searle

Scholars Award (2002), the MIT TR100

(2003), the Beckman Young Investigator

Award (2004), and the NSF CAREER Award

(2005). He has served as an Associate

Editor of the Annual Reviews of Physical

Chemistry since 2006.

progressively higher levels of precision. In

this talk, I will present recent developments

in the scientific laboratory on single

molecule studies in living cells, and

discuss how these advances in basic

science are enabling new opportunities for

clinical diagnostics well outside of the

laboratory setting.

Johannes Reisert studied Physics at

Siegen University (Germany) but soon

headed to Cambridge University (U.K.) to

pursue a Ph. D. in the Department of

Physiology. Thrown into a field Johannes

had never heard of before by his Ph.D.

supervisor Dr. H. R. Matthews, he began to

study how mouse and frog olfactory

receptor neurons (ORNs) code for odorant

stimulation, as well as investigating Ca2+

homeostasis mechanisms in ORNs and

The task of our senses is to explore, detect

and transform pertinent information in our

environment into electrical nerve signals to

guide behavioral decisions. For odorants,

this task is accomplished by olfactory

receptor neurons (ORNs) in the nasal cavity

that perceive volatile molecules carried by

the inhaled air and encode odorous

information into, ultimately, action

potentials that are conveyed to the

olfactory bulb in the brain. Odorant

DAY 01

Johannes REISERT

Associate Member Monell Chemical Senses CenterPhiladelphia, USA

How olfactory receptor neurons interpret our odorous world

Biography

how these contribute to odor adaptation.

After a quick detour via Germany to work

with Dr. S. Frings at Juelich, Johannes

joined the laboratory of Dr. K.-W. Yau at

Johns Hopkins University School of

Medicine in Baltimore to focus on Cl-

homeostasis mechanisms as well as

single molecule detection in ORNs. In

2005 he joined the faculty of the Monell

Chemical Senses Center in Philadelphia.

molecules bind to odorant receptors

located on ORNs, which is the first step in a

protein signaling cascade that leads to

gating of ion channels and cellular

depolarization. This presentation will

explore how ORNs and their signaling

components cope and make sense of the

vast variety of odorant molecules to

transduce them reliably (or not) into a time-

and concentration dependent output.

Dr Nic Lindley is a senior research director who has spent his entire professional life looking at how to understand and exploit microbial biodiversity as a source of innovation for industrial biotechnology applications. He has worked in the UK and in France, where he managed one of the most renowned public biotechnology research institute, before moving to Singapore to develop the new A*Star Biotransformation Innovation Platform and initiate research on how to translate the

Consumers are increasingly skeptical as to chemically synthesized flavor compounds but are also becoming increasingly interested in improving the taste and the nutritional content of their processed food. In addition the shift towards healthy foods is requiring additional efforts to make these products tasty. One of the challenges facing the food industry is to find alternative sources of existing, but also new molecules as natural flavours. Traditionally these compounds were often extracted from natural plant materials but these sources are often difficult to master both in quantity and quality of the desired molecules, as well as sometimes requiring extensive processing to remove other taste components which impart negative flavours to final products. The Biotransformation Innovation Platform at AStar is developing cell factory approaches to generate microbial strains able to synthesize high concentrations of specific flavour compounds. In order to exploit these high performance systems, we have to be able to screen and identify the right

DAY 01

Nic LINDLEY

Strategic Director A*STAR Biotransformation Innovation Platform, Singapore

Taste receptors as part of a screening process to discover new flavour molecules

Biography

academic scientific excellence into validated and innovative manufacturing, notably as regards high value-added flavours and fragrances for the food and consumer care sectors. He has worked extensively throughout his career with major biotechnology companies throughout the world and pioneered the metabolic engineering aspects associated with the cell factory concept (using microbial cells as a basis for complex synthetic reactions).

molecules. To facilitate this we have developed a taste receptor platform with two complimentary components. A high throughput screening system based on high efficiency cell transfection which allows us to couple any taste receptor variant to an artificial signaling pathway, and to thereby rapidly test its functional characteristics. The experimental setup allows us, for example to test receptor polymorphism and thereby determine how different variants of a given receptor associated with specific populations respond differently to key taste molecules. This experimental setup is associated to an in silico ligand binding modelling platform using structural understanding of the receptor’s biochemical affinity for taste molecules, to design ideal taste molecules and focus testing on the experimental screening platform in a given chemical space, thereby increasing the probability of finding new molecules. Some of the key technology factors involved in developing this taste receptor platform will be presented.

Dr. Toko is a Distinguished Professor of the Graduate School of Information Science and Electrical Engineering, Kyushu University, and was a dean for 2008-2011. He is now a Director of Research and Development Center for Taste and Odor Sensing, Kyushu University. He proposed a concept "to measure taste" about 30 years ago and succeeded in developing the taste sensor using lipid membranes, i.e. the electronic tongue first in the world. At present, over 400 taste sensor machines are used in food and pharmaceutical

Five years ago, IBM announced five innovations (Next 5 in 5), which are related to our five senses and will change our lives. Digital taste buds will help us to eat smarter, and computers have a sense of smell. In a similar way, four years ago, Google imagined a splendid world where we can record, transfer and realize smell anywhere and always.

Biochemical sensors which play the role of gustatory (taste) and olfactory (smell) senses have made rapid progress, and are named electronic tongues (e-tongues) and noses (e-noses), respectively. A taste sensor, which is a kind of e-tongue, has a property of global selectivity that implies a potential to decompose taste into five basic taste qualities (sourness, sweetness, bitterness, saltiness, umami) and quantify them; it was commercialized about 25 years ago [1-3]. It comprises several kinds of electrodes, on which a lipid/polymer membrane is pasted, and can discriminate, identify and quantify the taste. Generally speaking, e-tongues are designed to measure liquid samples using multiple sensor arrays with the low selectivity and high cross-selectivity instead of high selectivity to each chemical substance [4-6]. Each sensor electrode of the taste sensor is not specific to each chemical

DAY 01

Kiyoshi TOKO

Distinguished Professor Department of Electronics Graduate School of Information Science and Electrical Engineering, Graduate School of Systems Life Sciences

Director Research and Development Center for Taste and Odor Sensing Kyushu University, Japan

Biochemical Sensors for Taste and Smell

Biography

companies all over the world. He also succeeded in developing an electronic dog nose, the sensitivity of which is superior to dogs. He has directed and continues several government research projects in food, nanotechnology, and integrated sensing technology using biosensors and the taste/odor sensor. Due to these results, he won many prizes such as Prize for Science and Technology (MEXT) and Medal of Honor with Purple Ribbon. His research results are frequently on air in TV broadcast.

substance but to each taste quality in principle. The taste sensor has been applied to many kinds of foods such as coffee, tea, meat, rice, beer, milk, and wine, and has also been used to measure the taste of amino acids and medicines. It can provide a scale of taste, and can be utilized to produce new foods and medicines or control/monitor their qualities.

E-noses are biochemical sensors to detect smell, and then various types of materials and methods are reported: oxidized semiconductor, conductive polymer, transistor, quartz crystal microbalance (QCM), surface acoustic wave (SAW) and surface plasmon resonance (SPR). Among them, an “electronic dog nose” [7] based on SPR and an antigen–antibody interaction was developed. The detecting surface is chemically modified by a self-assembled monolayer containing oligo(ethylene glycol) and a surface-initiated atom transfer radical polymerization (SI-ATRP). As a result, TNT was detected at 5.7 pg/mL (ppt) using the optimized surface made by SI-ATRP and TNT–Ab by indirect competitive assay. In the near future, there will appear smart phones mounting the above kinds of e-tongues and e-noses.

Yanxia HOU has completed her Ph.D. in

Analytical Chemistry from Ecole Centrale

de Lyon (France). Then she spent a year as

postdoc at University of California San

Francisco/Touro University California

working on design of a biosensor to screen

small drug molecules against HIV. After,

she spent almost two years for a 2nd

postdoc at CEA-Leti in France, where she

worked on surface engineering of MEMS

and NEMS for electronic nose applications.

She is a permanent researcher at French

National Centre for Scientific Research

(CNRS) since 2008 in a joint laboratory

SyMMES (UGA-CEA-CNRS, UMR 5819)

located at Grenoble. In 2012, she

Nowadays, the development of novel

sensors that are able to provide reliable,

inexpensive and rapid analysis is driven by

the ever-expanding monitoring needs in

different domains, such as environment

monitoring (air/water quality control), the

detection of pollution or leaks of hazardous

materials, food safety and quality control,

and non-invasive medical diagnostics, etc.

Traditional techniques such as gas

chromatography and mass spectroscopy,

though accurate and reliable, are often

time-consuming and laborious to perform.

In this context, the electronic noses (eNs)

and electronic tongues (eTs) have emerged

as promising alternatives. They are

engineered to mimic the mammalian

DAY 01

Yanxia HOU-BROUTIN

Researcher Institut Nanoscienceset Cryogénie, CEA-Grenoble, CNRS, France

Optoelectronic Noses & Tongues for Imaging Smell & Taste

Biography

participated in the creation of a start-up

company Aryballe Technologies (Grenoble,

France) for the development of a universal

and portable optoelectronic nose. She is

also scientific advisor of the company. Her

major research areas are multiplexed

assays (biosensors and biochips) for

biomedical applications and electronic

tongues/noses for analysis of pure

proteins and complex mixtures in liquid or

in gas (VOCs) with potential applications in

diverse domains such as quality control of

food and beverages, food safety,

environmental monitoring, health-related

innovative technologies, etc.

olfactory system, consisting of an array of

low-selective sensors with

cross-sensitivity to different species in

complex mixtures and using advanced

mathematical procedures for signal

processing based on pattern recognition

and/or multivariate analysis. In this talk, I

will present recent developments in our

laboratory on the optoelectronic tongues

and noses based on an optical detection

system such as surface plasmon

resonance imaging. The obtained eTs and

eNs are capable of generating temporal

response with vivid 3D images as

“fingerprints” for the differentiation and

identificaton of the samples in liquid and in

gas.

Dr. Cho is a graduate of Stanford University where he

earned an M.S. in Materials Science and

Engineering, and a Ph.D. in Chemical Engineering

under the guidance of Professor Curtis W. Frank.

During his doctoral studies, Dr. Cho first gained an

interest in research at the interface of molecular

virology and biomaterials. The principal goal of his

thesis work was to develop lab-on-a-chip

technologies for analysis of viral protein interactions

with lipid membranes.

Dr. Cho then continued his postdoctoral training in

Professor Jeffrey S. Glenn’s group in the Division of

Gastroenterology and Hepatology at the Stanford

University School of Medicine. He applied these

engineering technologies to combat the Hepatitis C

virus (HCV), which affects over 150 million people

worldwide. His work has led to significant advances

for treating HCV, including new drugs currently in

preclinical or clinical trials. In addition, Dr. Cho has

DAY 01

Namjoon CHO

Associate Professor School of Materials Science and Engineering College of Engineering Nanyang Technological University, Singapore

Emerging Approaches to Fabricate Supported Lipid Bilayers: Moving Beyond Vesicles

Biography

pioneered a novel approach to liver tissue

engineering that has enabled an improved artificial

organ system for studying liver disease.

His passion for translational and regenerative

medicine has been recognized by several

prestigious international honors and awards from

the American Liver Foundation, Beckman

Foundation, and leading global universities and

companies including Chalmers University of

Technology and Roche Ltd. In 2011, Dr. Cho was

named an NRF Fellow by the Singapore National

Research Foundation, and was also appointed to a

Nanyang Associate Professorship. In addition to his

academic duties, Dr. Cho is the founder of

infollutionZERO, a global nonprofit organization

committed to building a green digital world for

future generations by eradicating infollution

(information + pollution) from the digital world.

Controlled self-assembly of model lipid membranes

at solid-liquid interfaces opens the door to a wide

range of applications across membrane biophysics,

biotechnology and medicine. Recently, we developed

the solvent-assisted lipid bilayer (SALB) method to

form supported lipid bilayers at interfaces. A key

feature of the SALB method is that the supported

bilayers form in an energetically favored scenario,

enabling bilayer fabrication on formerly intractable

surfaces like gold. Moreover, the process does not

require pre-formed precursor vesicles allowing for

arbitrary compositions. Aided by lipid-substrate

interactions, surface-adsorbed lipids in organic

solvent are rapidly converted into lamellar phase,

supported bilayer islands upon addition of aqueous

buffer solution. Lipid species in the aqueous solution

may attach to the bilayer islands and subsequently

rupture to form a contiguous, supported lipid bilayer.

Owing to the technically minimal requirements of

solvent-assisted lipid self-assembly, we have also

developed on-chip lipid microfluidics that take

advantage of the SALB method to form miniaturized

biomembranes with a rich complexity of components

reminiscent of natural cell membranes and that can

be utilized on a variety of substrates with different

atomic compositions and nanostructure

morphologies. In addition to the SALB method,

additional innovations have enabled streamlined

fabrication of supported lipid bilayers in fully

aqueous conditions by utilizing mixtures of

phospholipids with minimal preparation

requirements. In turn, all these capabilities should

further enable academic investigations related to

membrane biophysics and pharmaceutical drug

development efforts towards high-throughput lipid

membrane functional assays.

Prof. Jalali is the Northrop-Grumman

Endowed Chair and Professor of Electrical

Engineering at UCLA with joint

appointments in Biomedical Engineering,

California NanoSystems Institute (CNSI)

and Department of Surgery at the UCLA

School of Medicine. He received his Ph.D.

in Applied Physics from Columbia

This talk will describe two breakthrough

imaging modalities created at UCLA. The

first known as FIRE is the world’s fastest

fluorescent imaging modality with

applications blood analysis and brain

mapping. It labels fluorescence emission

from each pixel with a different radio

frequency (RF) tag and employs digital

wireless communication techniques to

create images. The startup company

DAY 01

Bahram JALALI

Professor and Northrop Grumman Opto-Electronic Chair

Department of Electrical Engineering

UCLA, USA Radio frequency multiplexed and time-stretch imaging techniques for cancer detection

Biography

University in 1989 and was with Bell

Laboratories in Murray Hill, New Jersey

until 1992 before joining UCLA. He is a

Fellow of IEEE, the Optical Society of

America (OSA), the American Physical

Society (APS) and SPIE. He was the

Founder and CEO of Cognet Microsystems,

a company acquired by Intel in 2001.

commercializing this technology was

recently acquired by BD Biosciences

(NASDAQ:BDX). The second technique

called time stretch imaging is the fastest

quantitative phase imaging modality. The

Artificial Intelligence (AI) augmented time

stretch microscope introduced in early

2016 has achieved label-free classification

of cancer cells in blood with record

accuracy and is rapidly gaining popularity.

Prof Luke P. Lee is a Tan Chin Tuan

Centennial Professor, Director of the

Biomedical Institute for Global Health

Research & Technology (BIGHEART), and

Associate President (International

Research and Innovation) at NUS.

He is a Fellow of the Royal Society of

Chemistry and the American Institute of

Medical and Biological Engineering.

Portable, low-cost, and quantitative nucleic

acid detection is desirable for point-of-care

diagnostics; however, current molecular

diagnostics often requires time-consuming

multiple steps and costly equipment. We

have developed smart mobile molecular

diagnostics system that leverages efficient

and dependable blood sampling,

automated sample preparation with digital

plasma separation, ultrafast photonic PCR

on chip, rapid optical detection of

multi-analyte nucleic acids and proteins,

and user-friendly systems integration with

wireless communication. The system

DAY 01

Luke P. LEE

Founding Director BIGHEART at NUSSingapore

Professor Departments of Bioengineering, Electrical Engineering and Computer Sciences, Biophysics Graduate Program, Berkeley Sensor & Actuator Center, UC Berkeley, USA

Smart Mobile Molecular Diagnostics System with Ultrafast Photonic PCR on Chip

Biography

Lee has over 350 peer-reviewed

publications and over 60 international

patents filed. He is a world-renowned

pioneer in nanobiophotonics, plasmonic

resonant energy transfer (PRET),

optofluidics, rapid photonic PCR,

microfluidics for quantitative life sciences,

and integrated molecular diagnostics

systems (iMDx).

includes a hand-held automated device

with an adaptive sample control module,

an optical signal transduction module, and

an interface to a smartphone making this a

reliable and field-applicable system for

point-of-care and on-demand diagnostics.

We envision that autonomous mobile

molecular diagnostic systems hold the

potential to breakthrough the number of

problems brought into the field of medical

diagnosis today and provide promising

foundations for future preventive

personalized medicine.

Dr. Dan Fletcher is the Chatterjee Professor

of Engineering Biological Systems at UC

Berkeley, where he also serves as Chair of

the Bioengineering Department and Chief

Technologist of the Blum Center for

Developing Economies. Dr. Fletcher

received a B.S. from Princeton University, a

D.Phil. from Oxford University as a Rhodes

Scholar, and a Ph.D. from Stanford

University as an NSF Graduate Research

Fellow. His bioengineering and biophysics

Light microscopy remains a critical tool for

disease diagnosis throughout the world.

Direct imaging of pathogens in blood,

sputum, and stool samples can provide a

rapid and definitive diagnosis of a broad

range of diseases, and specific labeling of

molecular components produced by or

induced by pathogens can provide further

information that directs treatment.

However, microscopy also requires skilled

technicians and equipment not routinely

available outside of centralized clinical

facilities. In recent years, mobile phones

have shown great potential to improve

DAY 01

Daniel FLETCHER

Professor and Chair Department of Bioengineering UC Berkeley, USA

Mobile phones as medical devices: Diagnosis of neglected diseases and beyond

Biography

research has been recognized with an NSF

CAREER Award, a Tech Award from the

San Jose Tech Museum, and a “Best of

What's New” designation by Popular

Science magazine. He served as a White

House Fellow in the Office of Science and

Technology Policy, is an elected Fellow of

the American Institute for Medical and

Biological Engineering, and was named

one of Foreign Policy's 100 Leading Global

Thinkers.

healthcare. As one example, the camera of

mobile phones can be converted into a

light microscope with sufficient resolution

to identify causative agents of disease and

detect molecular signatures. These mobile

phone microscopes, combined with the

continuously increasing computational

power of mobile phones, opens the

possibility of creating rapid point-of-care

diagnostic devices. This talk with describe

ongoing work that combines mobile phone

microscopy with both hardware and

software automation to expand access to

disease diagnosis.

DAY 02

Russell GRUEN

Executive DirectorNTU Institute for Health Technologies,

Professor of Surgery Lee Kong Chian School of Medicine Nanyang Technological University, Singapore

Saving lives through sensing: How new diagnostics will transform the care of severely injured

Biography

qualifications in health policy, medical ethics and business management from Harvard. He has over 180 publications spanning observational studies and clinical trials, systematic reviews, health policy and professional ethics, and many in journals such as The Lancet, JAMA and New England Journal of Medicine. He has received Australia’s premier clinician scientist awards, including the Practitioner Fellowship of the Australian National Health and Medical Research Council, and the John Mitchell Crouch Fellowship of the Royal Australasian College of Surgeons. From 2009-2015, as Director of Australia’s National Trauma Research Institute, his leadership responsibilities included large clinical trials and Australia’s National Trauma Registry, a binational trauma care program in India, the Lancet Commission in Global Surgery, and the WHO’s Global Alliance for Care of the Injured.

treatment protocols for the reception and

resuscitation of all seriously injured

patients. Given that not all patients exhibit

these changes, but that those that do can

deteriorate very rapidly, the need for early

and personalised treatments has become

apparent. Central to this mission is a new

generation of portable rapid diagnostic

tests that can be applied in the field and

guide early life-saving care.

Prof Gruen leads interdisciplinary health technology research at NTU, bringing together science and engineering expertise to focus on solving clinical problems. As a practising surgeon and clinician scientist, he is expert in health systems, ranging from biological processes and functional anatomy through to the organisation and delivery of health services. His clinical career of 25 years includes over a decade as trauma surgeon in Australia’s busiest trauma centres, and his trauma research interests span innovations for injury prevention, the control of bleeding, management of traumatic brain injury, and how trauma systems are best organised to provide time-critical care and promote long-term recovery. His work is interdisciplinary and highly translational, with strong emphasis on what works best for patients.

In addition to his clinical qualifications and PhD, Gruen has postdoctorate

Serious injuries sustained through falls,

interpersonal violence, road traffic and

workplace accidents, are a leading cause

of death and disability globally. In some

patients, tissue damage and haemorrhagic

shock cause a harmful cascade of

physiological changes that are apparent

within minutes of injury, lead to profound

coagulopathy, and are often fatal. Current

practices rely heavily on hospital-based

diagnostic tests, and on standardised

DAY 02

Hua ZHANG

Professor School of Materials Science and Engineering Nanyang Technological University, Singapore

Synthesis of Novel Two-Dimensional Nanomaterials for Sensing Applications

Biography

Dr. Hua Zhang obtained his B.S. and M.S.

degrees at Nanjing University in China in

1992 and 1995, respectively, and

completed his Ph.D. with Prof. Zhongfan

Liu at Peking University in China in July

1998. He joined Prof. Frans C. De

Schryver's group at Katholieke Universiteit

Leuven (KULeuven) in Belgium as a

Research Associate in January 1999. Then

he moved to Prof. Chad A. Mirkin's group at

Northwestern University as a Postdoctoral

Fellow in July 2001. He started to work at

NanoInk Inc. (USA) as a Research

Scientist/Chemist in August 2003. After

that, he worked as a Senior Research

Scientist at the Institute of Bioengineering

and Nanotechnology in Singapore from

November 2005 to July 2006. Then he

joined the School of Materials Science and

Engineering in Nanyang Technological

University (NTU) as an Assistant Professor.

On Sept. 1, 2013, he was promoted to Full

Professor. Until now, he has filed 68 patent

applications and 400+ papers with total

citation of over 36,100 and H-index of 91.

graphene-based composites, single- or

few-layer metal dichalcogenide

nanosheets and hybrid nanomaterials, 2D

metal-organic frameworks (MOFs), etc.

Then I will demonstrate their applications

in chemical and bio-sensors.

In this talk, I will summarize the recent

research on synthesis, characterization

and applications of two-dimensional (2D)

nanomaterials in my group. I will introduce

the synthesis and characterization of novel

low-dimensional nanomaterials, such as

DAY 02

Hung-Jen WU

Assistant Professor Department of Chemical EngineeringTexas A&M University, USA

A new device to probe complex multivalent

interactions on cellular surfaces

Biography

Dr. Hung-Jen Wu is an Assistant Professor

of Chemical Engineering at Texas A&M

University. He received his B.S. and M.S. in

Chemical Engineering from the National

Cheng-Kung University, Taiwan. He

completed his Ph.D. in Chemical

Engineering from Texas A&M University in

2006; then, he worked as a Postdoctoral

Fellow at the University of California,

Berkeley under Dr. Jay Groves's

supervision. After that, Dr. Wu worked in

the Nanomedicine Department at the

Houston Methodist Hospital Research

Institute, and was involved in developing

diagnostic tools for infectious diseases. Dr.

Wu joined Texas A&M University in the Fall

of 2013. Dr. Wu has received Kaneka

Junior Faculty Award in 2016. Dr. Wu's

research primarily focuses on the

development of quantitative tools for

diagnosis of diseases, including cancer

and infectious diseases.

lead to significant enhancement of protein

binding. I will first describe the influences

of multivalency and membrane dynamics

on lectin-glycolipid recognition. I will also

present a new sensing platform to

efficiently screen glycolipid receptor

candidates potentially involved in

hetero-multivalent lectin binding. Our

technology can conduct the multivalent

analysis in a highly accessible, flexible, and

inexpensive manner; thus, it will assist

scientists in understanding fundamental

principles of biological molecule

recognition, such as toxins, bacteria, and

virus, leading to new drug designs for

therapeutic purposes.

Proteins, bacteria, and virus often bind to

cell membranes via multivalent

interactions, leading to higher binding

avidity and specificity. Quantitative analysis

of analyte retention on cellular membranes

is essential in determining the rate of the

biochemical reactions and pathogenicity.

However, existing ligand-receptor binding

assays often fail to adequately describe

this essential process. We have developed

a plasmonic nanocube sensor coupled with

complex reaction analysis that enables us

to quantitatively explore multivalent protein

binding on cellular surfaces. We recently

observed that hetero-multivalency (a

protein simultaneously binding to two or

more different types of receptors) could

DAY 02

Shabbir M. MOOCHHALA

Distinguish Member of the Technical Staff Defence Medical & Environmental Research Institute DSO National Laboratories Singapore

Centre Director Centre for Molecular Diagnostics School of Applied Sciences Temasek Polytechnic Singapore

Biography

Dr Shabbir Moochhala concurrently holds joint

appointment as Centre Director, Centre for Molecular

Diagnostics, School of Applied Sciences at Temasek

Polytechnic as well as Distinguished Member of

Technical Staff at DSO National Laboratories. In

addition he is currently Adjunct Associate Professor

at Department of Pharmacology, Yong Loo Lin School

of Medicine, and National University of Singapore

and at School of Material Science & Engineering,

Nanyang Technological University. He has won both

local and international awards, including Sandoz

Grant for Gerontology (international)and European

Commission Grant (international) and is well

recognized both nationally and internationally in the

field of biomarker discovery, hemorrhagic shock,

neurotrauma, wound healing, gasobiology and

advance drug delivery systems. In 1997 he was

awarded the title of Charted Biologist (C BIOL) and

appointed to the membership to the Institute of

Biologist (M I Biol) in the United Kingdom. He has

also been invited to give seminars to Pre University

students and principals of secondary schools. Since

2001 till date he held 50 projects as PI/co-PI in

collaboration with various institutes in the areas of

combat care, human performance and drug delivery

systems. He has published more than 200 papers

(13 invited review papers); mostly in tier1-2 leading

cited international journals and has presented more

than 200 conference papers in local and

international conferences. He has written 9 chapters

for books and has filed three patents jointly with

other inventors from NUS & A*STAR Institutes. He is

often invited to peer review numerous scientific

paper for various local and internationally

accredited journals such as Lancet, Neurosurgery,

Journal of Clinical Investigation, European Journal

of Clinical Investigation, British Journal of

Pharmacology, Critical Care Medicine, Journal of

Pharmacy and Pharmaceutical Sciences, Life

Sciences, Singapore Medical Journal and Annals,

Academy of Medicine (Singapore). He is currently

Chairman, DSO ACUC (Institutional Animal Care and

Usage Committee), member of DSO IRB

(Institutional Review Board) and NHG DSRB-Domain

C IRB and Temasek Polytechnic IRB committees.

Novel Approaches for Detection of Exhaled

Gaseous Markersmarkers in exhaled breath. Most of these techniques

involve expensive instrumentation, high operational

cost, and long and tedious assay protocols.

Therefore, there is a strong demand for a facile and

point of care exhaled breath assay. This study

emphasizes on development of a sensitive and low

cost exhaled breath assay device for clinical

applications. Electrical, optical and microgravimetric

platforms are utilized in this study to identify and

quantify various bio-markers in the exhaled breath.

Heme-based receptors, organic recognition

molecules, polymeric materials are utilized for assay

of NOx, H2S, COx in exhaled breath. Their

multiplexed detection enabled finger print assay of

the various bio-markers in the exhaled breath.

Animal model studies (e.g. urban smoke inhalation

injury) have been carried out to validate the

developed sensor system. Subsequently, a prototype

for portable exhaled breath devices is illustrated

using the developed sensor system.

Gaseous markers in exhaled breath have been

associated with various diseases depending on their

in vivo levels .Detection of such exhaled gaseous

markers such as NOx (nitric oxide), H2S (hydrogen

sulphide), COx(carbon monoxide) is feasible and

could provide a non-invasive method for early

detection , continuous monitoring and management

of lung injury/diseases caused by battlefield trauma

and infectious/chronic illness. Currently, clinical

measurement of nitric oxide NOx in exhaled air is

proving to be a reliable marker of lung inflammation

and oxidative stress. However, current detector

systems are expensive, bench top system and only

detect a single analyte. Recently, several other

volatile gases, such as carbon monoxide COx and

H2S have been detected and correlated with various

types of pulmonary injury/disease. Unfortunately,

only a limited number of analytical techniques such

as chemi-luminescence, chromatography, and

spectrophotometry have been used for measuring

DAY 02

Mark PHONG

Asia Director Advanced Research Labs and Business Development L'Oréal Research & Innovation, Singapore

Fragrances and scents are a key to creating an augmented consumer experience in cosmetic products

Biography

Mark Phong currently holds multiple roles within the

research division of L’Oreal, where he is the Asia R&I

Director of the Advanced Research Labs and

Business Development. The L’Oreal Asia Advanced

Research labs are located in Tokyo, Seoul, Shanghai,

Singapore and Bangalore, usually as part of large

R&I Hubs or as standalone satellite centers. The

Advanced Research labs of L’Oreal are responsible

for the development of new cosmetics actives for all

categories of products in the L’Oreal portfolio. The

Advanced Research labs are also responsible for

cutting edge scientific research in biology,

chemistry, material sciences, biotechnology and

biophysics to advance our knowledge of skin and

hair properties in order to better understand the

specific needs of our diverse world-wide

consumers. He also heads the Asia-Pacific Business

Development and Strategic Foresight group, that is

responsible for identifying, sourcing and negotiating

the setup of new innovative external R&D

collaborations with organizations of all sizes (from

startups to other MNCs). Before L’Oreal, Mark has

extensive experience in the biotechnology and

Pharmaceutical industry, having worked in the past

as the VP of R&D at Curiox Biosystems, a successful

Singapore based Startup Company and as a Group

Leader with the American Pharmaceutical company

Eli Lilly & Co based in their Singapore Research

Center (Lilly Singapore Center for Drug Discovery).

Mark graduated with a double degree in

Bioengineering and Economics from the University

of Pennsylvania in the USA, followed by a PhD in

Pharmacology from the National University of

Singapore and finally an MBA from INSEAD in

France/Singapore.

molecules used in fragrance are able to elicit stress

reducing responses by activating specific olfactory

receptors in both the limbic nervous system as well

as the cerebral cortex. In addition to the well

appreciated role of scent molecules in driving

emotional responses, research has also shown that

certain fragrance molecules are also effective in

driving physiological responses in skin health,

including activating cell signaling pathways linked to

increased wound repair and keratinocyte

proliferation leading to potential benefits in skin

renewal. Such effects of fragrance modulation on

neural signaling pathways have been published

since the 1990s showing the link between olfactory

sensing and skin health including publications by the

Shiseido research labs on the

neuro-immuno-cutaneous-endocrine system. Much

of the impact of fragrance on cutaneous health

through these signaling pathways are linked to

modulation of systemic stress response including

levels of stress molecules such as DHEA, cortisol &

substance-P. Fragrances with the ability of triggering

physiologically positive effects in skin such as

wound repair and keratinocyte proliferation increase

the overall value perception of a top skin care

cosmetic product.

The cosmetics & personal care industry invests a

large amount of time, money and effort in creating

the ideal sensorial experience with their product. For

top cosmetics companies such as the L’Oreal Group,

a consumer centric design of products take into

consideration everything from the formula,

packaging, dispensing and deposition of a cosmetic

product onto the skin and hair, in line with

consumer’s expectations. Key elements considered

in the design of a formula include elements such as

the texture, smell (fragrance), and color. These

properties are key in eliciting emotional responses

from consumers and imparting feelings of

performance, exclusivity and luxury when the

product is used. Developing strong positive

emotional connections between a cosmetic product

and the customer has been proven by consumer

research to increase brand loyalty and repeat

purchase of a product.

Creating a fragrance that cues performance, and

elicits the right level of emotional response is among

the most difficult tasks in formula development. The

scientific basis of fragrance preferences, the likes

and dislikes in smell is an ever increasingly

important research topic and is key towards

developing winning cosmetic products. For example,

research studies have shown than certain olfactory

DAY 02

Gaurav SAINI

Principal Scientist / Senior Perfumer Flavors & Fragrances GCO Procter & Gamble Singapore Innovation Center, Singapore

Emerging challenges and opportunities in

fragrance design : An industry perspective

Biography

I am originally from India with a Bachelor in

Technology, Chemical Engineering from IIT

Bombay; followed with a Diploma in

Creative Perfumery at the P&G Perfumery

School. Over the last 25 years, I have had

the pleasure of working on several Procter

& Gamble businesses in different parts of

the world including India, Japan, US,

Belgium and now Singapore. Several

cleaning products brands such as Ariel,

Bold, Lenor, Downy, Joy, Wella Sebastien,

Fairy, Ambipur and Febreze have

fragrances designed by me. I have

partnered with P&G technologists to

design and bring fragrance delivery

systems/ technologies to market; which

deliver irresistible scent experiences

during product usage. I have had the

privilege of partnering closely with Flavors

& Fragrances industry spread all over the

world, including the big multinationals, the

niche naturals growers etc.

greater delight to the user and accelerate

the innovation cycle?. Going forward, what

are the new to world problems worth

solving using olfactive/ sensory science in

the next 10-20 years?

This talk discusses the challenges,

opportunities and the evolving role of the

perfumer in the fragrance industry. What

present challenges do perfumers have to

overcome while designing novel

fragrances? What are the new emerging

opportunities that allow perfumers and the

industry to improve fragrance design, bring

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Self Assembly of Protein‐Polymer Conjugate towards Nanocapsules, Proteinosome: A Sensing PlatformAmit Kumar KHAN, Bo LIEDBERG

Interdisciplinary Graduate School, Nanyang Technological University, Singapore

Unconventional Platforms for Detection of Growth Hormone Doping in SportsAntareep SHARMA, Kok On LEE, Bo LIEDBERG, Alfred TOK


Novel Approaches of Biomolecular Sensing: A Special Focus on Optical Sensors, Electronic Sensing and Point of Care Immunoassays Antareep SHARMA, Dorin HARPAZ, Kehan YE, Bo LIEDBERG, Robert MARKS, Alfred TOK


Visualizing Gene Expression in Live Cells with Versatile NanosensorsDavid YEO, Christian WIRAJA, Limin TAY, Chenjie XU

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore

‘Stack‐Pad’ a Novel Quantitative & Multiplex Point‐of‐Care Test (POCT) BiosensorDorin HARPAZ, Evgeni ELTZOV, Tim AXELROD, Luka FAJS, Supriya KUMAR, Raymond C.S. SEET, Alfred I.Y. TOK, Robert S. MARKS

School of Materials Science and Engineering, Nanyang Technological University, Singapore

Single Molecule Sensing of Protein DynamicsFrank VOLLMERLiving Systems Institute, University of Exeter

Development of a colorimetric assay for detection of matrix metalloproteinasesGarima GOYAL, Peng CHEN, Mrksich MILAN, Bo LIEDBERG

Interdisciplinary Graduate School/NTU‐Northwestern Institute for Nanomedicine, Nanyang Technological University, Singapore

Improving sensitivity of bacterial assays by optimizing peptide substrate for membrane proteases using a bottom‐up proteomic approachGaurav SINSINBAR, Gudlur SUSHANTH, Kevin METCALF, Mrksich MILAN, Nallani MADHAVAN, Bo LIEDBERG


Lab on Paper using Conjugated Polymers for Sensing ApplicationsGopal AMMANATH, Meng YU, Yildiz CHE, Umit Hakan Palaniappan ALAGAPPAN, Bo LIEDBERG


Enabling biocompatible electronics for sensing applicationsJieun KO, Luong T. H. NGUYEN, Abhijith SURENDRAN, Kee Woei NG, Wei Lin LEONG

School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore

An Auto‐Regeneratable Electrochemical Aptasensor for Continuous Monitoring of Biomolecules Enabled by Ion Concentration PolarizationLin JIN, Sun TAO, Dinh‐Tuan PHAN, Chia‐Hung CHEN

School of Biomedical Engineering, National University of Singapore

Diffraction limited sub‐wavelength imaging with a scattering lens for biomedical applications Manish VERMA, Quan LIU


Photonic Technology based Sensor for Biomedical ApplicationsMayur Kumar CHHIPA, SRIMANNARAYANA, K.

Electronics And Communication, K L University, Guntur, A.P, India

E2—The First Nonviral Protein Nanocage as Pickering EmulsifierMridul SARKER, Sierin LIM


Detection of Cancer Biomarkers Utilizing Gold Nanoparticles and Reduced Graphene OxidePeng CHEN, Bo LIEDBERG


High Shear Stresses under Exercise Condition Destroy Circulating Tumor Cells in a Microfluidic SystemSagar REGMI, Afu FU, Sierin LIM, Kathy Qian LUO


Identification of ATP‐competitive PAK4 inhibitors by biophysical assayHongyan SONG, Wui Siew TAN

Materials Processing and Characterisation Department, A*STAR, Institute of Materials Research and Engineering

Highly Sensitive, Label‐Free Detection of 2,4‐Dichlorophenoxyacetic Acid using an Optofluidic ChipXueling FENG, Gong ZHANG, Lip Ket CHIN, Ai Qun LIU, Bo LIEDBERG


Metasurface‐based structured illumination for super‐resolution imagingZhengji XU, Ting YU, Yu LUO, Dao‐Hua ZHANG, Qilong TAN, Shuang ZHANG

School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore

Multi‐Responsive Fluorescence Sensing Based on a Donor‐Acceptor‐Donor Molecule for Highly Sensitive Detection of Water and CyanideCangjie YANG, Xiaochen WANG, Zhigang XU, Mingfeng WANG


Sustained and Cost Effective Silver Substrate for Surface Enhanced Raman Spectroscopy Based Biosensing Jian JU, Quan LIU


jay t. groves · division director's fellow in the physical biosciences division at lawrence...

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