List%of%Chemicals%for%2016417 Aneexure4A

S.No. Chemicals Name 1 1"#naphthyl"acetate2 1,4"Dioxane3 100"bp"ladder"marker"upto"5Kba4 100bp"DNA"Ladder5 100bp"Ladder,"1kb"ladder6 100g"o#Phenylenediamine7 10x"TBS"Buffer8 10x"TE9 1kb"DNA"Ladder10 20"bp"DNA"Ladder11 20"bp"ladder"marker12 2#Mercaptoethanol13 2#Naphthyl"acetate14 3100"Pop#6"EA15 3730"buffer""10X"with"EDTA,"500"ml16 50bp"DNA"Ladder17 6X"DNA"loading"dye18 6X"Loading"buffer19 6X"Loading"buffer"&dye20 6X"Loading"buffer,"15%"Ficoll21 Acd3"purified"145#2C1122 Acetone"500ml"EMPLURA"MERCK23 Afl"III24 AFl#III#1000unit25 Agarose26 Agarose"Low"EEO"100g27 Agarose,1KG28 Albumin29 Alkaline"Phospahate30 Amber"centrifuge"tube"conical"bottom"

(15ml)31 Amber"centrifuge"tube"conical"bottom"

(50ml)32 Ammonium#bt"Carbonate33 ANTI"GADPH34 Anti"Human"cd3"zeta"chain"35 Anti#"IL#17"purified"antibody36 ANTI"JAGGERED37 Anti"mouse"HR38 Anti"mousecd3"zeta"chain"39 ANTI"NOTCH"

40 Anti#CD341 Apo"I42 Auto"Bilirubin"(T&D)43 Barbitone"buffer44 BD"Falcon"50"ml"Conical"Tube"Sterile,"

Nonpyrogenic"with"Screw"Cap"45 BD"round"POLYSTYRENE"TEST"TUBE"

12X75MM,"5ML,"46 BDT"sequencing,"v3.1"cycle,"100"rections47 Big"Dye"terminator48 Bis#Acrylamide49 B#Naphthyl"acetate50 Boric"acid51 Boric"acid"molecular"grade52 Brad"ford"reagent53 Brij#2354 Bromophenol"blue55 BSA"(Bovine"Serum"Albumin)56 BSA"Bovine"Serum"albumin57 BseJI"(BsaBI)58 BseJI"(BsaBI)"2000units59 Bsl"I60 Bsr"I61 BstN"I62 Cac8I63 Calcium"chloride64 Calcium"Green"Dye65 Capillary"array,"96x50cm"each66 Casein67 Casein"100g68 CCR7"BV4214B1269 CD11aFITC"M17/470 CD11Bfitc"M1/7071 CD#19FITC"1D372 CD25PE"pc6173 CD2874 CD28"human75 CD29PE""HM"β1#176 CD3"human77 CD34APC"RAM3478 CD49dPE"R1-279 CD81PE"C15.680 CD8aFITC"11B1181 CD9BIOTIN"53#6.782 cDNA"synthesis"Kit

83 Cell"Culture"Plates"Sterile"Tissue"Culture"Treated"Polystyrene,"12#well,flat#bottom"with"lid"

84 Chaps#85 Charchol"activated86 Charge"Lighter"Gas"for"burner87 Chemisol88 Chloramphenicol89 Chloramphenicol#500gm90 Chloroamphenicol91 Chloroform92 Chloroform"MB"Grade93 Chloroform"molecular"grade94 Chloroquine"diphosphate#"salt95 Cholestrol96 Chymotrypsin97 CLONED"AMV"RT"750U"TRANSCRIPTASE98 Cocaltchoride99 Coenzyme"A100 Colloidal"stain101 Color"Change"0°"Labtop"Cooler"for"0.2"ml102 Commaric"Brilliant"blue103 Conjugated"Control"IgG104 Control"IgG105 Coomassie"Blue106 Coper"sulphate107 Creatinine108 Cryo"box"rack109 Crystl"Violet110 culture"flask111 Custom"Antibody"Services112 Cyclohexane113 Cytochrome"C114 DAPT115 Dde"I116 ddNTPs#DNA"Sequencing"Chemical"

(Sanager"based)117 Dehydrin"Antibody118 Delta"methrin119 DEPC120 DEPC"treated"water121 DEPC"water122 Dessicant123 Dialysis"membrane124 Diethyl"ether

125 Diethyl"Ether"500ml"126 Diethyl"pyrocarbonate127 Diethylamine"(CDH)128 Disodium"hydrogen"Ortho"phosphate"129 Disodium"hydrogen"phosphate"dehydrate,

130 Disodium"sodium"hydrogen"phosphate131 Dithiobis"2#nitrobenzoic"acid132 DMSO"(dimethylsulphoxide)133 DNA"ladder134 DNA"ladder#20bp,50bp,100bp,1kb135 DNA"Polymerase136 DNA"stabilizer137 DNAase138 DNase#Free"RNase139 DNASeI140 DNAZOL"REAGENT"141 Dneasy"to"remove"DNA"contaminants142 DNTP"mix143 dNTP"mix"2.5"mM"each144 dNTP"mix,"10mM"(2.5mM"each)"4x200µl145 dNTP"Mix#10"mM"(2.5"mM"each),"1000"μl146 dNTP"mixture147 DNTPs148 DNTPs"Mix(2.5mM"each)149 DNTPs"Mix(2.5mM"each)150 Dopamine151 DPEC152 DPIP153 Dpn"II154 DPX"mountant""for"microscopy155 Dra"I156 Drabkin"reagents157 Dream"taq158 Dream"Taq""Master"Mix"(genetix)159 DTT"(Dithiothreitol)160 DTT"(Di#thiothrietol)161 Eco"RV162 Eco31I"(BsaI)163 Eco88I"(AvaI)164 Eco88I"(AvaI)"1000units165 EDTA166 EDTA"(Ethylenediaminetetraacetate)167 EDTA"500g168 EDTA"molecular"grade

169 EDTA"vacutainer170 EDTA,"Free"Acid171 EmaraldAmp"Max"PCR"Master"Mix172 Eosin"yellow173 Eosin"Yellowish"stain"#"for"microscopy174 Eosine"Yellowish"Merck175 Epoxomicin176 ETBR#Ready"to"use177 Ethanol178 Ethanol"500ml179 ETHANOL"ANHYDROUS180 Ethanol"molecular"grade181 Ether182 Ether"500ml183 Ethidium"bromide184 Ethidium"bromide"molecular"grade185 Ethyl"alchol186 Ethyl"ether187 Ethylene"glycol"(EGTA)188 Exonuclease189 Exonuclease"I"E.coli"#4000units190 Exonuclease#1191 ExoSAP#IT®"For"PCR#Product"Clean#Up"#"

200"ul192 Expansin"Antibody193 FAST"AP194 Fast"blue"B"salt195 Fast#Media®"Amp"XGal196 FCS197 Fetal"Bovine"Serum"(FBS)198 FicollImmunohistochemistry:"UltraCruz"

Mounting"Medium199 FITC"labeled"Anti"Rabbit"secondary"IgG"

antibody200 FITC"tagged"antibodies201 Fok"I202 Folic"acid203 Forceps204 Formaldehyde205 Formaldehyde"Soln."500ml206 Formic"acid207 FoxP3"PE""MF#23208 G6PD"diagnosis""kit209 Gamma"secretase"DAPT210 Geimsa"stain

211 Gene"ruler"100"bp"DNA"ladder212 GeneCompTM"101"Competent"Cells"

Strain:"DH5α,">1x108213 GeneRulerTM"50"bp"DNA"Ladder"ready#to#

use"50μg214 GeneRuler™"50"bp"DNA"Ladder"50μg215 Genomic"DNA"isolation"Kit"(from"filter"

paper)216 Gentamicin"antibiotic"solution217 Gentamycin218 Giemsa's"Stain"Sol"125ml"MERCK219 Glacial"Acetic"acid220 Glacial"Acetic"Acid"500ml221 Glucose222 Glucose"6"phosphate223 Glutathione224 Glutathione"oxidised"(GSSG)225 Glutathione"oxidized226 Glutathione"reduced227 Glycerol228 Glycerol"(AR"Grade)229 Glycerol"Anhydrous"2.5"Ltr"Emplura230 Glycine231 Glycogen232 Golgi"Stop233 Good"view"dye234 GR#1PE"1A8235 green"taq"PCR"master"mix236 GTP237 Guanindine"Hydrochloride238 H2O2"(Hydrogen"Peroxide)239 HaeIII240 Hcl241 HDL"cholestrol242 Hema"3"System"(includes"Fixative"and"

Solutions"I"and"II)243 HEPES"100ML244 HEPES"buffer245 HEPES"free"acid246 HES#1247 Hi#Di"Formamide"Bottle"#25ml248 High"mol."Wght"marker249 Hydrochloric"acid250 Hydrochloric"Acid251 Hydrochloric"acid"molecular"grade

252 HYDROCHLORIC"ACID,500ML"253 Hyoxanthine254 IFN#g"APC255 IL#12FITC"TC11#18H10256 IL#17PE257 IL#4PURIFIED258 IL#6259 IL#9PURIFIED"D8402E8260 Imidazole261 Immersion"Oil262 IMMERSION"OIL(Microscopy"Grade)263 Immidazole264 Indicator"tape"for"steam"autoclave265 Insuline"sringes266 Iodoacetamide"(IAA)267 Ion"exchange"resins268 IPG"strips269 IPTG"(Isopropyl"β#D#1#

thiogalactopyranoside)270 Iso"octane"(HPLC"gr)271 Isoamyle"alcohol272 Isopropanol273 Isopropyl"alcohol274 Isopropyl"alcohol"(HPLC"gr)275 Isopropyl"thiogalacto"pyranoside276 J.S.B.#"STAIN"SOLUTION"No.#1277 J.S.B.#"STAIN"SOLUTION"No.#2278 K2HPo4279 Kanamycin280 Kappa"Hi#fi"Hot"start"readymix281 Kappa"HiFi"Hotstart"Ready"Mix#500rxn282 Kcl283 KH2Po4284 KMC8285 KOD"plus286 L#"arginine287 L"GLUTAMINE"100X"100ML288 L#arginine289 LB"AGAR290 LB"agar291 LB"media292 L#Canavaline293 L#Citrulin294 L#GLUTATHIONE"REDUCED"295 LightCycler480"Probes"Master

296 L#NAME297 LongAmp"Taq"DNA"Polymerase"#"500"units

298 Low"molecular"weig.""marker299 LPS300 LS"COLUMN301 Lysozyme302 Lysozyme"molecular"grade303 M#30"D"Diluent"20"Ltr304 M#30"E#Z"Cleanser"100ml"X"1305 M#30"P"Probe"Cleanser"17ml"X"1306 M#30"R#Rinse"20"Ltr307 magnaetic"BEADS"CD11c308 magnaetic"CD"4"309 magnaetic"CD"8a310 Magnesium"chloride311 MaximaTM"Hot"Start"PCR"Master"Mix312 MbolI313 Metaphor"Agarose"(DNase/RNase"free)"

for"small"fragment"separation"(<10bp)314 Methanol315 Methanol"(HPLC"grade)316 Methanol"500ml317 Methnaol318 Methylene"Blue319 Methylene"Blue"Powder"MERCK320 Methylene"blue"stain"#"for"microscopy321 Methyline"blue"322 Mgcl2323 micro"tips"200ul324 micro"tips"2ul325 MicroAMp"Fast"Reaction"cap"(8cap/strip)326 MicroAMp"Fast"Reaction"tubes"

(8tubes/strip)327 MicroAmp"Optical"Adhesive"film328 Microcrystalline"cellulose329 Microtube"Tough"Spots"3/8''"diameter330 Mineral"Oil331 MluC"I332 Mnl"I333 Molecular"Biology"Grade"Water334 Mono"Dansyl"Cadavarin335 Mouse"CD#27"V450"LG.3A10336 Mouse"IL#10"PE"JES5#16E3"Mouse"337 Murine"IFN#gamma"

338 Mwo"I339 Na2HPO4340 Nacl341 NaCl"molecular"grade,"342 NADP343 NEB"5#alpha"F"Iq"Competent"E.coli(High"

Efficiency)"#"6x0.2"ml344 NGS/Metagenomic/"Bioinformatic"Service

345 Ni#NTA"agarose"beads346 Nitric"acid347 Nitric"Oxide"Inhibitor":"1,3#PBITU,"

Dihydrobromide348 Nitroblue"tetrazolium"chloride349 Nitrocellulose"Membrane350 NOR"NOHA351 Nuclease"free"water352 NUCLEASE#FREE"WATER"1"LITER353 Octopamine354 O#DIANISIDINE355 Oligo"(Primer)356 O#Phenylenediamine357 Optisafe"gel"hand"gel358 Oregon"Green"AM359 Ortho"phosphoric"acid360 Para"Nitrophenyl"acetate"Acetonitrile361 Parafilm362 PBS"12X1L363 PBS"with"Tween"(PBS#T)364 PCR"marker"mixture365 PCR"purification"kit366 Pecoll367 PENICILLIN"STREPTOMYCIN"SOL"100ML368 PEPES369 Percoll370 Permethrin"standard371 pH"strip372 Phenazonium"Methosulphate373 Phenol374 Phosphate#Buffer"Saline"(PBS)375 Phosphoric"acid376 Phusion"Hi#Fidelity"taq"polymerase377 PMS378 PMSF379 p#Naphthyl"acetate

380 Poneau"Dye381 POP7"Polymer"for"3730XL"DNA"analyzer382 Potacium"dichromate383 Potasium"acetate384 Potasium"chloride385 Potasium"nitrite386 Potassium"acid"phosphate387 Potassium"dichromate"EMPLURA388 Potassium"Dicromate389 Potassium"phosphate,"monobasic"500g390 Pottasium"Dichromate391 Pottasium"phosphate392 Powder"free"Nitrile"Gloves"(Small,"

Medium,"Large)393 Pre#mixed"green"dye"PCR"Master"Mix",2X,"

1000rxn394 Primer"395 Primer"5000bp396 Propanol#2"MB"Grade397 Propionic"acid"250ml398 Propoxur"Pestanal"(2#isopropoxy#phenyl)399 Propoxur,"Acetylthiocholine"iodide400 Propyl"alcohol"(AR,"HPLC)401 Protease"Inhibitor"cocktail402 Protease"inhibitor"cocktail"tablet403 Protease"inhibitors"set404 Protein"Estimation"Kit405 Protein"Marker"(Colored)406 Protein"Molecular"weight"markers407 Proteinase"K408 Proteomics"(iTRAQ,"LC#MS,"MALDI)"

services409 Pst"1"3000"units410 PVDF"membrane411 PVDF"membranes412 Rapamycin413 Rapigest414 Real#time"PCR"master"mix"(SYBR)#200"x"50"

ul415 recombinant"proteins"FGF416 recombinant"proteins"GMCSF417 recombinant"proteins"IFNg418 recombinant"proteins"IL#10419 recombinant"proteins"IL#12p40420 recombinant"proteins"IL#1beta

421 recombinant"proteins"IL#21422 recombinant"proteins"IL#4423 Restriction"enzyme"424 Restriction"Enzyme"Alu"I,"Pst"I,"Sal"I425 Reverse"Trancriptase426 Ribolock427 Ribozol"RNA"extraction"reagent428 RNA"purification"kit429 RNAlater430 RNAse"Inhibitor431 RNase#Free"DNase432 RPMI"1640"1X10L433 RPMI"1640"with"HEPES,L#glutamine434 RPMI#1640"culture"media"with"L#

glutamine"and"25mM"HEPES","without"Sodium"bicarbonate

435 rSAP436 Safeskin"Purple"Nitrile"gloves#9.5"inch"

Length"(Small)"437 Saponin438 Sca#1FITC"D7439 SDS440 Sephadex"G#75441 Serological"pipettee442 Serotonin443 SGOT444 SGPT445 SHEATH"FLUID"446 SHEATH"FLUID"447 Shirp"alkaline"phosphatase448 Shrimp"Alkaline"Phosphatase,"

Recombnant"(rSAP)"#1000Units"(1U/ul)449 Skimmed"Milk450 Skimmed"milk451 Slide"box452 Slide"staining"kit453 Sodium"acetate454 Sodium"Acetate455 Sodium"azide#100gm456 Sodium"Bicarbonate457 Sodium"carbonate458 Sodium"chloride459 Sodium"dihydrogen"phosphate460 Sodium"dithionate461 Sodium"Dodecyl"Sulphate

462 Sodium"hydrogen"carbonate463 Sodium"hydroxide464 Sodium"hydroxide"molecular"grade465 Sodium"Hypochlorite466 Sodium"Hypochlorite"EMPLURA467 Sodium"Hypochlorite"LR468 Sodium"metavanadate469 Sodium"nitrite470 Sodium"Phosphate"(dibasic)471 Sodium"Phosphate"(monobasic)472 Sodium"phosphate,"dibasic,"

anhydrous500g473 Sodium"potasium"tartarate474 Sodium"Succinate475 Sorbitol476 Spilfyter"labsoakers477 Sringe"filter"0.45um478 Sringes"10ml479 Sringes"1ml"480 Sringes"50ml481 SsiI"(AciI)482 SsiI"(AciI)"200units483 Stat"3"inhibitor484 StepUp"100bp"DNA"ladder,"100loads,"

50µg485 Sterile"H2O486 Sterile"PBS487 Sterile"TE488 Streptavidin#PhycoerythrinFITC"Eat2"

KMC8489 Streptomycin490 Streptomycin"sulphate491 Sucrose492 Sulpho"salicylic"acid493 Sulphuric"acid494 Sulphuric"Acid"(commercial)495 Syber"green"master"mix496 SYBR"Green"Master"Mix(roche)497 Tag"Man"Probes/primer498 Taq"DNA"polymerase"499 Taq"DNA"polymerase"Pfu(for"large"

fragment)500 Taq"DNA"polymerase"with"standard"Taq"

buffer#2000"units501 Taq"DNAPolymerase"(3U/µl)"1000U

502 Taq"I503 TCA504 TCRabAPC"H57#597505 TEMED506 TEMED"

(Tetramethylethylenediamine)507 Terific"Broth508 Tetra"acetic"acid509 Tetracycline,50mg/ml,20ml510 TETRAMETHYLBENZIDINE"511 TGF#beta"1"512 Top"Taq"Master"Mix"Kit513 Toptaq"DNA"polymerase514 Torin"2515 Total"Protein516 Tough"spots517 Triglycerides518 Tris519 Tris"(hydroxymethyl)"aminomethane520 Tris"500g521 Tris"Base522 Tris(Hydroxymethyl)Aminomethane523 Tris#HCL"molecular"Grade"(Dnase/RNase"

free)524 Triton"X"100525 Trizol526 Trypan"Blue527 Trypsin"proteomic"grade528 Tween"20529 Tyramine530 Universal"Methylated"DNA"Strand531 Urea532 Uric"Acid"533 VIRAL"RNA"isolation"Kit534 Viral"RT#PCR"Kit535 Vsp"I536 Water"HPLAC"grade537 Water"HPLC""grade"DNase/RNase"free"538 Xmn"I539 Xylene"(GR"Grade)540 Xylene"Gr"2.5"Ltr541 Β#Mercaptoethanol

List%of%Consumables%for%2016417 Aneexure4B

S.No. Consumables Name 1 Cryo"Tags"(30X19)2 Cryo"Tags"(32.5X12.7)3 Forceps4 Freezing"cardboard"cryobox5 Hand"onTM"Nitrile"Examination"Gloves6 Hb"microcuvatte7 Indicator"Bacillus"strips"for"steam"autoclave8 Indicator"tape"for"steam"autoclave9 K2EDTA"vacutainer10 Lansets11 Medium"safe"skin"nitrile"Gloves12 Nitrile"Gloves"large13 Pricking"needles14 Small"safe"skin"nitrile"Gloves15 Tough"Spot16 Tough"Tags"17 Whatman"Filter"Paper18 Whatman"Filter"Paper"3CHR"19 Whatmenn"filter"paper"no."(12.5"cm)

List%of%Glasswares%for%2016417 Aneexure4C

S.No. Glasswares Name

1Beaker""(25ml,"50"ml,"100ml,"250ml"&"500ml)/"Glassware

2 Beakers#100ml,250ml,500ml,1000ml3 Carboy"with"stopcock

4Conical""Flask"(25ml,"50"ml,"100ml,"250ml"&"500ml)/"Glassware

5 Conical"flask#100ml,250ml,500ml,1000ml6 Conical"flasks7 Conical"Tubes"(15"ml)8 Conical"Tubes"(50"ml)9 Culture"vials(Borosil)10 Giemsa"stain"sol.

11Glass"measuring"cylinders#25ml,20ml,100ml,250ml,500ml,1000ml

12 Glass"slides13 Glass"Tubing"(Borosil)14 Glasswares15 Measuring"cylinders

16Micro"Slide"75"x"25mm."1.1mm."Froste"D"(50/Pack)

17 Micro"slides

18Micro"Slides"75x25"mm"(Thickness:"1.2mm)

19 Narrow"Mouth"bottle,"1000ml20 Narrow"Mouth"bottle,"500ml21 Reagent"bottles"1000ml22 Reagent"bottles"500ml

23Round"bottom"flask,"100ml,250ml,500"ml,1000ml,2000,3000"ml

24Schott"Duran"Bottles#50ml,100ml,250ml,500ml,1000ml

25 Stirring"rod26 Test"tube27 Test"tubes#2ml#10ml28 Wide"Mouth"bottle,"1000ml29 Wide"Mouth"bottle,"500ml

List%of%Kits%for%2016417 Aneexure4D

S.No. Kits Name1 "Mouse"10#PLEX"FOR"CYTKINE"DETECTION:"

IL1b,"IL4,"IL6,"IL10,"IL12p"40","TNF"a,"IL17,"IFN"g,"MIP1,"IL2

2 Ni#NTA"spin"columns3 2D"starter"Kit4 7#AMINOACTINOMYCIN"D"(7#A"1"MG.5 AgenCourt"Ampure"XP"Kit6 Arginase"detedtin"kit7 Axyprep"blood"genomic"DNA"miniprep"kit8 Buffer"kit9 cDNA"synthesis"Kit10 Dengue"NS1"Ag"+"IgG/IgM"Kit11 DNA"Blood"Mini"Kit"(250)

12DNA"isloation"Kit"from"animal"tissues,"whole"blood,dried"blood"spot,250"rxns

13 DNA"isolation"kit14 DNase"1000"Units15 DreamTaq"DNA"polymerase"500"units16 Dynabeads"mRNA"direct"micro"kit17 E#Gel"size"select"2%18 Elisa"Kit19 Falcivax"Kit20 Falcivax"Pf/Pv"RDT"kit

21 Flacivax"Divice22 G6PD"kit23 G6PD"kit"Span"Diagnostic24 G6PD"Test"Kit25 Gel"extraction"kit#250rxns26 Gel"purification"kits27 GeneJet"Gel"extraction"kit,"250"prep28 GeneJet"PCR"purification"kit29 GeneJET"Whole"Blod"Genomic"DNA"

Purification30 Genescan#500(LIZ)"Size"Std"Kit"EA31 GST"resins32 High"Sensitivity"DNA"Kit"for"Bioanalyzer33 Human10#PLEX"FOR"CYTKINE"DETECTION:"

IL1b,"IL4,"IL6,"IL10,"IL12p"40","TNF"a,"IL17,"IFN"g,"MIP1,"IL2

34IL12,"IL10,"TNF#alpa,"IFN#Gamma"ELISA"KITS

35 ION"314"CHIP"KIT"V2"EACH36 ION"316"CHIP"KIT"V2"(4"PACK)"EACH37 ION"318CHIP"KIT"(4"PACK)"EACH38 ION"PGM"ENRICHMENT"BEADS"EACH39 ION"PGM"SEQUENCING"400"KIT"EACH40 ION"PGM"TEMPLATE"OT2"400"KIT"EACH41 ION"SPHERE"QUALITY"CONTROL"KIT"EACH42 ION"TORRENT"1#16"barcode"kit43 ION"Torrent"DNA"STANDARD"&"Primer"

Premix"kit44 ION"TORRENT"RNA"Library"preparation"kit45 ION"total"RNA"–Seq"Kit46 iTaq"SybrGreen"supermix47 Malaria"diagnosis"kit

48Maxima"SYBR"Green"qPCR"master"mix,"200"reacts

49 MTT"cell"proliferation"kit

50NEBNext"Fast"DNA"Fragmentation"and"Library#Prep"Set"for"Ion"Torrent"#"50"rxns

51 Notch"Receptor"Interaction"Antibody"Sampler"Kit

52 Parachek"Divice53 PCR"Cloning"Kit54 PCR"Purification"Kit,250rxns55 pGEM"T"Easy"Vector"System

56Phospho#Akt"Pathway"Antibody"Sampler"Kit

57 Pierce"GST"Protein"Interaction"Pull"Down"Kit,"25rxn

58Pierce"HIS"Protein"Interaction"Pull"Down"Kit,"25rxn

59 Plasmid"DNA"isolation"kits

60PLATINUM"PCR"SUPERMIX"HIGH"FI"100"REACTIONS

61 PROSTAGLANDIN"E2"PARAMETER"ASSAY"KIT

62 Protein"digetion"kit(in"gel)63 Q"PCR"kit64 QIAamp"DNA"Blood"Mini"Kit"(250)65 QIAamp"DNA"mini"kit,"250prep66 QIAamp"Viral"RNA"mini"kit67 Qiagen"One"step"RT#PCR"Kit68 QIAquick"PCR"Purification"KIT69 QIAquick"PCR"Purification"Kit(250)70 Quibet"quantification"kit71 Quick"Start"Protein"Assay"Kit72 Real"time"PCR"kit73 RevertAid"premium"first"strand"cDNA"

synthesis"kit,"50"reacts74 RNA"In#Situ"Hybridization"Kit75 RNA"isolation"kit76 RNA"purification"kit77 Silver"stain"kit78 SMARTer"cDNA"synthesis"kit79 SYBR"FAST"qPCR"kit80 Taq"DNA"polymerase"3U/ul81 TGFb"single"plex

82Transcript"Aid"T7"high"yield"transcription"kit

83 Urea"detection"kits84 Whole"Blood"DNA"Isolation"Kit85 Whole"Genome"Amplification"Kit86 Kinore#Glo"ADP#Glo"Kinore"Assay"Kit

List%of%Plasticwares%for%2016417 Aneexure4E

S.No. Plasticware Name1 0.2"micron"syringe"filters2 0.2ml"96"well"plates3 0.2ml"PCR"8#Strip"Tubes"with"attached"

Domed"Caps,4 0.2ml"PCR"Tube"with"domed"cap

5 0.2ml"PCR"tube"with"flat"cap,1000/Pk6 0.2ml"PCR"tubes7 0.2ml"PCR"tubes,"flat"cap8 0.2ml"PCR"tubes,"Strips9 0.45"micron"syringe"filters10 0.5ml"Micro"Centrifuge"Tube11 0.5ml"Micro"Tube"Box12 0.5ml,0.6ml"low"retention"tubes"with"cap13 0.6ul"microtubes14 1"ml"pipette"tips15 1.5"ml"eppendorf"tubes16 1.5ml"Micro"AmberTube17 1.5ml"Micro"Centrifuge"Tube18 1.5ml"Micro"Tube"Box19 1.6ml"Graduated,"MCT20 10"ul"pipette"tips21 10µl"Bulk"Tip22 10µl"Filter"Tip23 1000"ul"Tips24 1000ul"barrier"micro"tips25 1000ul"filter"tips26 100ul"barrier"micro"tips27 100ul"barrier"micro"

tips,Filtered,sterile,Low28 10ul"barrier"micro"tips,Filtered,sterile,Low

29 1250ul"barrier"micro"tips,Filtered,sterile,Low"retention,

30 15"ml"falcon"tubes31 15ML"POLYPROPYLENE"CONICAL"

CENTRIFUGE"TUBE,"32 2"ml"eppendorf"tubes33 200"µl"Bulk"Tip34 200"µl"Filter"Tip35 200"µl"Micro"Tips36 200#1000µl"Micro"Tips37 200ul"barrier"micro"tips38 200ul"barrier"micro"

tips,Filtered,sterile,Low39 200ul"pipette"tips40 200ul"Tips41 50"ml"flacon"tubes42 5#10"ul"Tips43 5#10µl"Micro"Tips44 96"well"plates"Flat#bottom"with"lid

45 Aerosol"Barrier"UniversalTips,0.5#10µl"46 Aerosol"Barrier"UniversalTips,100#1000µl"47 Aerosol"Barrier"UniversalTips,1#200µl"48 Amber"centrifuge"tube"conical"bottom"

(15ml)49 Amber"centrifuge"tube"conical"bottom"

(50ml)50 Amber"wide"mouth"bottle"500ml"(6"Pack)51 Autoclave"bags52 BD"Falcon"50"ml"Conical"Tube"Sterile,"

Nonpyrogenic"with"Screw"Cap"53 BD"round"POLYSTYRENE"TEST"TUBE"

12X75MM,"5ML,"54 Beaker"500ml"Tpx"(6/Pack)55 Beaker#100ml,250ml,500ml,1000ml56 Blood"Clooection"Tubes"Sterile"Vaccum"K2"

EDTA"13x75mm,"3ml"(BD)57 Blood"Clooection"Tubes"Sterile"Vaccum"

Vacutainer"Slica"Clot13x75mm,"4ml"(BD)58 Brown"dark"colour"1.5#2.0"ml"MCT59 Card"Board"Cryobox"81"Place"Box"for"

1ml/2ml"Vials"(10/"Pack)60 Cardboard"Cryobox61 Cardboard"Cryobox#100"place"box"for"1.0#

2ml62 Cell"Culture"Plates"Sterile"Tissue"Culture"

Treated"Polystyrene,"12#well,flat#bottom"with"lid"

63 Cell"Culture"Plates"Sterile"Tissue"Culture"Treated"Polystyrene,"6#well,flat#bottom"with"lid"

64 Centifuge"tube""15"ml65 Centifuge"tube"50"ml66 Centifuge"tube,"Conical"bottom,"15ml67 Centifuge"tube,"Conical"bottom,"50ml68 Centricons"for"protein"concentration69 Centrifuge"tube"17"x"120mm,"15"mL70 Centrifuge"Tube"Conticla"Bottom"15ml"PP"

sterile"(500/"Pack)71 Centrifuge"tubes"2"ml72 Centrifuge"vials73 Conical"Centrifuge"Tube,PP/HDPE,"15ml,"74 Conical"flask"1000ml75 Conical"flask"250ml76 Conical"flask#100ml,250ml,500ml,1000ml

77 Conical"Tube"Rack78 Conical"Tubes"(15"ml)79 Conical"Tubes"(50"ml)80 Cryo"Box81 Cryo"Box"1.0"&"1.8ml"81"Places"(4/Pack)82 Cryo"box"rack83 Cryo"spots"for"tubes"labelling84 Cryo"vials"1.2"Ml"(internal"thread)85 Cryo"Vials"Sterile"1.8ml"(500/Pack)86 Cryotags#1.28"x0.5087 Cryovial"sterile"threaded"(1.0ml)88 Cryovial,"0.5ml,"Natural,"PP,"Sterile,"Bulk,89 Culture"Flask"(25cc"sterile;"Non"vented;"

Closed"cap)90 Culture"Flask"(25cc"sterile;"vented)91 Culture"Flask"(75cc"sterile;"Non"vented;"

Closed"cap)92 Culture"petri"plates"(sterile)93 Disposable"basins"94 Disposable"filters"1"ltr95 Disposable"filters"250"ml96 ECO"Illumina"48well"plates"(Real"time"

plate)97 ELISA"PLATES98 Eppendorf""Tube#"1.5mL99 Eppendorf"(1.5ml)100 Eppendorf"stands101 Eppendorf"Tubes"(1.5ml,"2"ml)102 Falcons"15"ml103 Filter"discs104 Filter"tips105 filters".22um106 Forcep107 Freezer"Change"Rack"for"1.5#2ml"tubes96"

places,44x99x141108 Frosted"Slide"109 Funnel110 Gloves"(Nitrile)"Powder"free111 Gloves"Rack112 Glucose"201"Microcuvettes"(4x25)113 Hand"onTM"Nitrile"Examination"Gloves114 Indicator"tape"for"steam"autoclave115 Insuline"sringes116 Internal"thread117 IsoFreeze"PCR"Rack"

118 LightCycler®"480"Multiwell"plate"96,"white"

119 LightCycler®"480"Multiwell"Plate"96,"white"with"sealing

120 LightCycler480"Multiwell"Plate121 Magnetic"beads"for"stirrer"with"its"

magnetic"rod122 Magnetic"stand"for"1.5#2ml"tubes123 Maxymium"recovery"tips,""bulk"0.5#10µl"124 Maxymium"recovery"tips,""bulk"100#

1000µl"125 Maxymium"recovery"tips,""bulk"1#200µl"126 Measuring"Cylinder"(25ml,"50"ml,"100ml,"

250ml"&"500ml)/"Glassware127 Measuring"scoop"(100)128 Measuring"scoop"(5)129 Measuring"scoop"(50)130 Membrane"filter"Holder#47mm131 Micro"centrifuge"tube"0.5ml132 Micro"centrifuge"tube"1.5ml133 Micro"centrifuge"tube"1.5ml134 Micro"centrifuge"tube"2.0ml135 Micro"centrifuge"tube,"Amber"colour,"

0.5ml136 Micro"centrifuge"tube,"Amber"colour,"

1.5ml137 Micro"centrifuge"tube,"Amber"colour,"2ml138 Micro"Cetrifuge"Tube"1.5ml""(500/Pack)139 Micro"Pipette"box"(Small"&"Large)140 Micro"Pipette"tips"1000"µl"500"No141 Micro"test"plate142 Micro"tip"(0.2#10microlitre)143 Micro"tip"(10#200microlitre)144 micro"tips"1000ul145 micro"tips"200ul146 micro"tips"2ul147 Micro"Tube"box,"1.5ml148 MicroAmp®"EnduraPlate™"Optical"96#Well"

Fast"Clear"Reaction"Plates"with"Barcode"

149 MicroAmp®"EnduraPlate™"Optical"96#Well"Fast"Clear"Reaction"Plates"with"Barcode"

150 MicroAmp®"Fast"8#Tube"Strip,"0.1"mL

151 MicroAmp®"Fast"Reaction"Tube"with"Cap,"0.1"mL

152 Micro#Capillary"Tubes153 Micropestle154 Microtips".1#10ul155 Microtips"100#1000ul156 Microtips"2#200ul157 Microtube"Tough"Spots"158 Midi"Sub"Prep"System#Size"13cmX"25cm159 Mini"Kit,"50prep160 Minisart"nylon0.45"ul"25"mm161 Multipurpose"labeling"tape"(mixed)162 Narrow"Mouth"bottle,"1000ml163 Narrow"Mouth"bottle,"500ml164 Non"Filter"Microtips"(1000"ul)165 Non"Filter"Microtips"(200"ul)166 Non"skirted"96"well"plate"standard167 Non#Filter"Microtips"(10"ul)168 Non#Filter"Microtips"(100"ul)169 Oak"Ridge"Centrifuge"Tube"10ml"PP"(Pack"

of"24x12"Piece)170 Parafilm171 Parafilm"dispenser172 Parafilm,"4"x125',"1Pk173 Parafilm,"4"x250,"1"PK174 Pasteur"Pipette"(1ml"Sterile)175 Pasteur"Pipette"Rubber"Bulb176 Pasture"Pipette"1ml"Strile"(500/"Pack)177 Pasture"Pipette"3ml"Strile"(500/"Pack)178 PCR"0.2ml"tube"rack"with"cover179 PCR"Plates180 PCR"tube"racks181 PCR"Tubes182 PCR"tubes"183 PCR"Tubes"&"Caps,"RNase#free,"0.2"ml(8"

stript"formate)184 PCR"tubes"(Acer"+"Thermo"cycler)185 PCR"tubes"caps186 PCR"Tubes(0.2ml"flat"cap"tubes)187 PD"10"empty"gravity"flow"columns188 Petridishes189 Petridishes#90mm190 Pipette"mates191 Pipette"Stands192 Plastic"Beaker

193 Plasticware"(tips"&tubes194 Powder"Free"Nitrile"Exam"Gloves#

Small,Medium,Large"size195 Pricking"Needle196 Rack"for"Microtube,"0.5ml"X24197 Rack"for"Microtube,"1.5ml"X24198 Rack"for"Microtube,"1.5ml"X48199 Racked#96,768/pk200 Radiation"Sterilized"Falcon"(15ml,50ml)201 Reagent"bottles"1000ml202 Reagent"bottles"500ml203 Reagent"Reservoir#25ml204 Recombinant"proteins"IFNg205 retention,Racked#96,960/pk,"low"

retention"tips206 Reversible"Racks"#96"places,12x8"array207 Safeskin"Purple"Nitrile"Gloves"Medium"

(100/Pack)208 Scissors209 Serological"pipette""sterile"(2ml,"5ml,10ml)

210 Serological"pipettee211 Serological"pipettes"(10ml)212 Serological"pipettes"(1ml)213 Serological"pipettes"(2ml)214 Serological"pipettes"(5ml)215 SHEATH"FLUID"216 Slide"box217 Slide"staining"kit218 Spilfyter"labsoakers219 Storage"boxes"for"eppendorf"tubes220 Storage"vials"2ml221 Storage"vials"5"ml222 Supreno"SE,"Nitrile",Powder"Free","

Small,100"PC/bx223 Syringe"Filter224 Syringe"filters#"0.2#0.45um"size225 Syringes226 Syringes"10"ml227 Syringes"1ml"228 Syringes"50"ml229 Syringes#5#10ml230 TC"Flask"25"cm2"(with"doubel"seal"cap)231 TC"Flask"75"cm2"(with"doubel"seal"cap)232 Test"tubes#2ml#10ml

233 Thermal"paper234 Thin#walled,"frosted"lid,"RNase#free"PCR"

tubes"(0.2"mL)235 Thin#walled,"frosted"lid,"RNase#free"PCR"

tubes"(0.2"mL)236 Tips"0.5#"10"ul237 Tips"100#1000""ul238 Tips"1#200"ul"ul239 Tough"spots240 Tubes"&"Caps,"50/Bg"241 Tubes"stand"for"15ml#"50ml"falcon"tubes242 Utility"tray#360X310X130"MM243 Vaccutainer"(Heparinized)244 Vacutainer"(EDTA"coated)245 Vacuum#driven"Filters"MCE"hydrophilic"

membrane#Pore"size"0.22"mm,"250"ml"volume,"12No/pack

246 Wide"Mouth"bottle,"1000ml247 Wide"Mouth"bottle,"500ml

List%of%Sequencing%for%2016417 Aneexure4F

S.No. Sequencing1 Genome"sequencing,"Transcritome"

Sequencing:"Illumina/Roche/PAC"BIO"Services

2 Sequencing3 Standard"single(PCR)"sequncing4 Standard#seq"plate(Multi"primer)5 I#D6 2#DE7 In"Solution"Digestion8 In"Gel"Digestion9 LC#MS/MS10 MALDI#TOF11 iTRAQ12 TMT