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EEC code IT01 bu ITALIAN MEDITERRANEAN WATER BUFFALO The Best Genetics Available in Italy ARTIFICIAL INSEMINATION CENTER

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EEC code IT01 bu

ITALIAN MEDITERRANEAN WATER BUFFALO The Best Genetics Available in Italy

ARTIFICIAL INSEMINATION CENTER

The buffalo has always being part of Italian culture as a matter of fact, according to some historians the buffalo has being in our country since the Longobardi eras, instead according to others it has being around since the roman’s. In Italy it has always being used to produce milk out of which, as you all know, we mostly make mozzarella cheese. In the rest of the world instead the buffalo has being used mainly to help the man working the land, and in some country it still is. Do to his versatility we can find in the world, like we do also for the bovine species, different type of breeds that satisfy the different needs. We have the Italian Mediterranean buffalo, the Indian buffalo (we can find the Carabao in southern Asia, instead the Arni we can find it more in Vietnam, China , Philippine, Taiwan, Indonesia and Thailand), the African Black Buffalo, the Bison (we can find it in United States of America although there is also an European cousin), the Murrah buffalo (originally comes from the some regions of India more precise from Punjab and Haryana and we can find it too in Pakistan), then similar to the Murrah we also have the Nili-Ravi buffalo (we can find it mainly in Pakistan and in India in the region of Punjab). So we can say that up to some years ago the buffalo was more of a Asian/European prerogative, instead in this last few years we have seeing an interest in it from other country like Australia, Canada and United State, this because is a rustic animal ad has the capability of adapting to any kind of climate. The world buffalo population is estimated to be around 174 million heads of which 97% is in Asia (concentrate for the most in India, China and Pakistan), 2% in Africa (for the most part in Egypt) and the remaining is spread in Europe (Italy, Romania and Turkey) and South America (Brazil, Venezuela, Colombia and Argentina). As you can see that is a great potential, very interesting for all of the breeders in particular the Mediterranean’s. the buffalo are animals that, for us that we work with them daily we know for sure, can give great satisfactions. Just by looking at these numbers makes you understand the great potential there is and the future that we can build.

di Arturo Dr. Casali

With the September 2015 genetic index published by ANASB, COFA confirms is leadership in the Italian genetic selection. If you look at a the indexes you may see that in the first 120 indexed buffalo 40 are out of COFA’s sire, quite an excellent result.

We got an extraordinary result on the heifers, as you can see in the first 120 indexed heifer 66 are daughters of COFA’s sires.

ZERBIO with the September 2015 Genetic Index he confirms his fantastic data, ZERBIO stays the # 1 bulls in the world, extraordinary is his milk and Kg of protein index. His daughter are exceptional animals with great structure and dairyness, he’s for sure a great sire and will live a strong effect on the Italian genetic selection. OTELLO after his first excellent index, he manages to upgrade is milk index to a +338,3 placing himself within the first 10 best sire for milk index, furthermore his daughters are animals with excellent udders. MALANDRINO III after so many years of leading the chart he had to leave the way to ZERBIO. GOKU is confirming with his 212 daughters all of the positive traits for milk production and % of fat and protein with a 98% reliability. LUCKY with his 187 daughters and 98% reliability, confirms his strength in transmitting high production with good structure, udder and feet & legs. CAESAR is confirming with his 262 indexed daughters a sire very well balanced for production, milk quality and excellent morphology. O-B-ONE with his 434 daughters and a 99% reliability is a sire very well balanced on his production traits and gives daughters with exceptional udders. TORCINO has confirm his good data for production. ETTORE is conforming well balance on production. SPARTACUS is confirm his good data for production. JAFAR one of our pioneer of our genetic, with his 173 daughters is confirming is accuracy on his production traits.

With the support of ANASB and the cooperation of our fellow farmers, we have being able to achieve all this extraordinary result on the Italian buffalo selection. Giovanni Binotti Director of COFA A.I. stud

di Galli Prof. Cesare

COFA Sire 40 Sire from other AI stud 13 Netural mounting 67

Total: 120

COFA Sire 66 Sire from other AI stud 26 Netural mounting 28

Total: 120

Zerbio

Malandrino III

Goku

THE BUFFALO IN THE WORLD

by Mattia Casali

Mediterraneo

137

104

92

130

115

121

124

124

123

110

117

121

116

117

80 92 104 116 128

O-B-ONE da Izano IT019500215620

PKM +204,40 Milk +906,90 F Kg +59,70 F % -0,44 P Kg +39,50 P % -010,

Data ANASB Septembre 2015

Foto 20-09-2006

OTT

AVIA

– Z

ERBI

O’s

Mat

erna

l Gra

ndda

m

Ow

ner:

Cap

rare

lli F.

lli

Data ANASB December 2014

Sire MALANDRINO III VG 89

TEOSIO IT003900021247 PKM 169,10 Dati ANASB 2015

I.G.P. PKM 84,55 Set. 2015 PIA IT003900001886

2 03.06 214 2805 7,9 4,6

Dam MALANDRINO III VG 89

TERZA IT003900021248 PKM 169,10 Dati ANASB 2015

n° year days Milk F% P% OTTAVIA IT003108000842

1 02.07 270 4623 7,2 4,3 2 04.09 270 6460 8,4 4,5 3 06.04 270 5345 7,4 4,1

n° year days Milk F% P% 2 03.11 270 4069 7,4 4,4

TER

ZA -

ZER

BIO

’s D

am

Udder close up

T059990136387 ZER

BIO’s D

aughter

Zerbio’s daughters distinguish themselves as animals with a big structure and for the excellent udder composite

Daug. 98 Herds 30 General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Daug. 129 Lact. 242 Herds 36 97% R

Daug. 262 Lact. 670 Herds 50 98% R

Born on 06-10-2000 IT019500148631 Owner: Az Agr Massari F.lli Izano Cremona Italy

PKM +51,60 Milk 208,20 F Kg +15,60 F % -0,05 P Kg +9,70 P % +0,00

Pictured 10-05-2005 Born on 07-10-2002 IT019500348511 Owner: Az Agr Massari F.lli Izano Cremona Italy

PKM +96,30 Milk +424,00 F Kg +29,00 F % -0,18 P Kg +18,30 P % -0,07

Daug. 187 Lact. 465 Herds 52 98% R

Foto 10-05-2005

Sire VG 85 TONINO II° CE 24521

NAPOLI CE 28216

PKM 4,49 Data ANASB 2012 NONTISAZI MAI CE 18428

4 1998 270 2884 8,1

Dam MALANDRINO CE 24552

INSEGNA CR 630 G+ 82

n° year days Milk F% P% COLORIZIO CE 28255

1 1995 270 2713 9,7 4,7 2 1996 270 3726 8,0 4,5 3 1997 263 3664 7,7 4,7 4 1999 270 3944 8,1 4,7 5 2000 270 3849 7,8 4,6 6 2001 270 4161 8,0 4,6

n° anno gg Latte G% P% 6 1998 270 4379 7,2 4,0

100

109

104

118

114

103

102

115

101

108

111

115

109

109

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Daug. 190 Herds 43

Data ANASB September 2015

Sire VG 85,5 MALANDRINO CE 24552

MASTER IT054CR003C202

CR 1595

Dam MALANDRINO CE 24552

INSEGNA CR 630 G+ 82

n° year days Milk F% P% COLORIZIO CE 28255

1 1995 270 2713 9,7 4,7 2 1996 270 3726 8,0 4,5 3 1997 263 3664 7,7 4,7 4 1999 270 3944 8,1 4,7 5 2000 270 3849 7,8 4,6 6 2001 270 4161 8,0 4,6

n° year days Milk F% P% 6 1998 270 4379 7,2 4,0

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length 97

105

91

126

123

112

123

107

117

119

122

125

121

124

80 92 104 116 128

Daug. 130 Herds 43 Data ANASB June 2015

TESTED BULLS

Data ANASB September 2015

Data ANASB June 2015

89

127

107

119

125

98

129

122

122

134

133

97

130

131

80 92 104 116 128

O-B-ONE da Izano IT019500215620

Daug. 434 Lact. 1033 Herds 61 99% R PKM +27,30

Milk +141,20 F Kg +8,80 F % -0,10 P Kg +5,00 P % -0,07

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Data ANASB Sept. 2015

Sire VG 89 ALDESTE SA 42196

JAFAR IT054CR003C167 PKM 45,20 Data ANASB 2015 INSEGNA CR 630 G+ 82

6 2001 270 4161 8,0 4,6

Dam ALESSIO CR 721 VG 89

RESICA CR 1574 VG 86

n° year days Milk F% P% Az 35 CE 24597 G+ 81

1 1998 270 3237 8,0 4,5 2 2000 270 5178 7,4 4,2 3 2001 270 5118 7,6 4,3 4 2002 270 4510 7,9 4,6 5 2004 270 5027 7,8 4,4

n° year days Milk F% P% 6 1998 270 4379 7,2 4,0

Foto 20-09-2006

Daug. 313 Herds 50 95% R

Available Straws 0,50cc and 0,25cc

RES

ICA

ow

ner:

Mas

sari

F.lli

Ow

ner:

Mas

sari

F.lli

O-B-ONE daughter

RESICA - Dam of O-B-ONE

Reserve Champion at the interregional fair in Eboli Salerno 2007

Data ANASB Dicember 2015

101

101

101

101

101

101

88 100 112 124 136 148

Daug. 14 Lact. 24 Herds 7 83% R

Born on 27-03-2007 IT019990352839 Owner: Agricola Caffi srl Sesto Cr/Se Cremona Italy

PKM +77,30 Milk 338,30 F Kg +13,06 F % +0,04 P Kg +25,00 P % -0,04

Pictured 10-05-2005 Born on 20-06-2003 IT061000104834 Owner: Agricola Vitale Ciorlano Caserta Italy

PKM +13,50 Milk +234,40 F Kg -2,40 F % -0,87 P Kg +5,30 P % -0,23

Daug. 72 Lact. 168 Herds 22 95% R

101

101

101

101

101

101

88 100 112 124 136 148

Foto 10-05-2005

General Score Structure Feet & Legs Udder Composite Stature

General Score Structure Feet & Legs Udder Composite Stature

Sire VG 86,5

IT061000008273

Dam RADAR SA 49426

IT021AP357C126 G+ 84

n° year days Milk F% P% GINESTRA AP 0000009

1 03.01 270 3909 6,0 4,0 2 04.07 252 3913 8,7 4,6 3 05.08 270 3975 7,7 4,4 4 06.09 245 3473 7,1 4,4 5 07.10 270 4479 7,4 4,2 6 08.11 270 4026 9,3 4,7

n° year days Milk F% P% 4 05.10 270 2187 8,4 4,5

Sire EX 90 LT 6997 EX 93

CIRCE 1 IT059000066852

PKM 15,80 Data ANASB 2015 LT 6710 G+ 84

n° year days Milk F% P% 6 07.10 252 5963 9,1 4,4

Dam IT051CR035C004 VG 88

COROLLA IT019500176494

n° year days Milk F% P% CR 1469 G+ 81

1 02.06 270 2317 9,0 4,8 2 03.09 270 3205 8,3 4,5 3 05.01 260 3162 7,8 4,4 4 06.04 270 3236 8,4 4,4

n° year days Milk F% P% 8 10.01 270 3382 9,2 4,9

Data ANASB September 2015 Data ANASB September 2015

TESTED BULLS

PKM +108,63 Milk +487,45 F Kg +33,70 F % -0,20 P Kg +20,38 P % -0,09

100

106,5

103,5

123,5

118,5

109

122

102

121,5

117

115

111

117

117,5

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Data ANASB September 2015

Sire VG 88,5 Malandrino ITCE000024552 VG 89

MALANDRINO III IT054CR003C174

PKM 169,10 Data ANASB 2015 RESICA ITCR000001574 VG 86

n° year days Milk F% P% 3 05.01 270 5118 7,6 4,3

Dam PKM 153,60 Sept 2015 LUCKY IT019500348511 VG 87

River PERLA IT077990042258 G+ 83 PKM 96,30 Data ANASB 2015

n° year days Milk F% P% Az 3002 IT059990044055 G+ 84

1 02.07 270 4465 7,4 4,7 2 03.10 270 4285 8,0 4,8

n° year days Milk F% P% 3 04.10 270 4712 7,0 4,4

Foto 20-09-2006

LUC

KY O

wne

r: M

assa

ri F.

lli

MAL

AND

RIN

I III°

Ow

ner:

Mas

sari

F.lli

Data ANASB Dicember 2014

PKM +161,35 Milk +764,65 F Kg +45,10 F % -0,37 P Kg +32,05 P % -0,10

BLACKSTAR #1 among the young bulls has a fantastic Expected Morphology Index, as a matter of fact by looking at the graph, it comes to attention it’s positive impact on all of the morphology traits. In particular you can see that it’s very strong on the Front and Rear Udder Attachment, Stature and Body Length. Very good also on Foot & Legs and Fetterlock Strength, traits that are very important on Buffalo selection. BLACKSTAR is a sure bet because is improving production and all of the morphology traits.

BLACKSTAR is the #1 among the YOUNG BULLS. The best bull in A. I. with the Sire and Grand Sire

with Production & Morphology indexes.

+F

Foto 22-10-2009

Milk +936,30 F Kg +46,70 F % -0,40 P Kg +37,50 P % -0,06

Milk +538,20 F Kg +27,75 F % -0,25 P Kg +21,25 P % -0,06

BATIMAN great sons of O-B-One with a Dam that has an outstanding production.

Milk + F Kg + F % - P Kg + P % +

Milk + F Kg + F % - P Kg + P % +

Data ANASB September 2015 Data ANASB September 2015

Sire VG 85 JAFAR IT054CR003C167

O-B-ONE IT019500215620

PKM 27,30 Data ANASB 2015 RESICA CR 1574 VG 86

n° year days Milk F% P% 3 05.01 270 5118 7,6 4,3

Dam IT019500131505

FAEMA IT019500263675

n° year days Milk F% P% IT051CR035C058 G+ 84

1 02.02 270 3185 7,2 4,4 2 03.04 242 3461 8,0 4,5 3 04.05 260 3839 6,6 4,4 4 05.05 270 3410 8,6 4,5 5 06.05 270 3635 8,4 4,4

n° year days Milk F% P% 4 05.07 270 3670 7,5 4,4

O-B-One

Sire VG 85 JAFAR IT054CR003C167

O-B-ONE IT019500215620

PKM 27,30 Data ANASB 2015 RESICA CR 1574 VG 86

n° year days Milk F% P% 3 05.01 270 5118 7,6 4,3

Dam LINUS VA 1008

TAITI IT003900021197

n° year days Milk F% P%

1 02.01 270 3809 6,9 4,5 2 03.07 270 4940 7,9 4,6 3 05.00 270 4535 7,9 4,7 4 06.03 270 4303 7,2 4,2 5 07.05 270 5242 7,4 4,3 6 09.09 270 4978 5,5 4,0

n° year days Milk F% P%

O-B-One

Data ANASB September 2015 Data ANASB September 2015

PKM +55,93 Milk +261,33 F Kg +17,63 F % -0,12 P Kg +10,40 P % -0,07

99

117

112

118

119

103

126

110

125

125

121

97

122

121

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Sire VG 85 JAFAR IT054CR003C167 VG 89

O-B ONE IT019500215620 PKM 45,20 Data ANASB 2015

PKM 27,30 Data ANASB 2015 RESICA ITCR000001574 VG 86

n° year days Milk F% P% 3 05.01 270 5118 7,6 4,3

Dam PKM 255,40 Sept 2015 Malandrino III IT054CR003C174 VG 89

UDEA IT003990003934 VG 86 PKM 169,10 Data ANASB 2015

n° year days Milk F% P% OLIMPIA IT003108000786 G+ 80

1 02.03 270 4880 7,8 4,8 2 03.10 270 5094 7,2 4,6 3 05.01 270 4637 8,2 4,4 4 06.06 270 4627 8,2 4,4 5 07.08 270 3627 7,8 4,5

n° year days Milk F% P% 1 02.04 270 3959 8,7 4,8

Foto 20-09-2006

MAL

AND

RIN

O II

I° O

wne

r : M

assa

ri F.

lli

O-B

- One

Ow

ner :

Mas

sari

F.lli

PKM +141,35 Milk +673,20 F Kg +39,10 F % -0,25 P Kg +28,05 P % -0,06

CICLOPE is a progeny bulls that has excellent Expected Indexes, in particularly the Expected Morphology Index, because it has a linage with big structure, exceptional udder and excellent teats treats, dimension and position, very strong on the body length, furthermore the daughters are animals well structured with bug dairyness traits. A sure promise for the Italian selection.

CICLOPE bull in A.I. with the Sire and Maternal Grand Sire that has

the Production and Morphology Indexes.

+F

Data ANASB Dicember 2014

Data ANASB September 2015

Az. Agr. Caprarelli F.lli al centro Alessandro e Giuseppe

For the first time in the history the #1 buffalo for PKM comes from the Latina province. Born and raised in the farm of Giuseppe and Alessandro Caprarelli, one of the historic farm of the Agro Pontino, the buffalo az 427 IT059990136247 has succeeded in being at the first place for PKM on the September genetic index 2014 published by ANASB but this is not all, this extraordinary animal has succeeded in confirming his status of #1 buffalo for PKM by staying at the first place also on the publication by ANASB of the genetic index of March and September 2015. This buffalo, with extraordinary production is scored G+84 and it’s a daughter of Zerbio, best sire for PKM and milk index. Right now this buffalo is pregnant with Ombra e Nisiuno, could be a future sire to put on AI, certainly it would have an extraordinary and unique pedigree. .

di Arturo Dr. Casali

COFA Centro Bovino e Bufalino

ISIDORO is a new progeny bull with extraordinary morphology and genetic traits. The mother is a Zebio’s daughter from the Caprarelli herd in Latina, with a PKM of +173,9 that place her on the 49th place in the genetic index of September 2015. The father Allbalck is a progeny bull with an index, Isidoro is a

DIRECTION is a good Goku’s son with a very good mother, JANA daughter of Malandrino III with a PKM of +179,6 that place her on the 33rd position on the genetic index of September 2015. On Direction’s pedigree we can find a fusion between the very strong traits of Goku in production and %

GOKHAN is a new AI bull son of Goku, a sire with great production traits and excellent deviation on the % of fat and protein. His mother, Udea, is a daughter of Malandrino III and she place at 2nd place on the September 2015 genetic index. Udea is an exceptional buffalo on production and very strong on

bull very strait with a good structure, dairyness and feet & legs traits, it was scored VG 85 at 2 year of age. A good investment in order to improve the genetic in the farm.

of fat and protein, and the capacity of Malandrino III to pass on to a strong production combined with great structures.

morphology, she was scored VG 86. Gokhan is for sure one of the best young sire available for AI to help the farmer improves the genetic in the herd.

APPIO is a new AI bull son of Malandrino III, a sire that we all know well capable of passing on to his progeny great potential in production, the mother on the other hand, AZ3356, is the daughter of a fantastic buffalo, AZ221, that produced on her 2nd lactation Kg4043 of milk with 8,3% of fat and 4,5% of protein.

AGRIPPA is a new AI bull sons of Jesce Sole a sire with big productive traits, and with a mother, az 895, daughter of Goku a sire that is very strong on % of fat and protein. The mother is a buffalo with good production scored G+83. This pedigree is very interesting because it unites the up grating a the

Appio is a sire with good structure and stature, with very good dairyness, feet & legs very correct, strong fetterlock and a excellent strength in the dorsal line. This sire is for sure the bull to use if you want to improve your functional morphology.

productivity traits of two extraordinary sires. Agrippa is a young bull morphologically correct with good structures and dairyness, for sure a very interesting bulls to improves productivity.

With the 215/16 progeny bulls COFA makes another step forward on the Italians genetic selection, proposing a group of new buffalos with very important and significant characteristics, to start with all of them are sons of AI sire and 4 out of 5 have the mother too out of a AI sire. Furthermore we tried to select bulls that could satisfy the needs of the breeders in terms of improving the productivity and the morphology. In this group we can find sire like Isidoro and Appio with strong trait concerning the structure and dairyness, we have also sire like Gokhan, Direction and Agrippa with strong traits in dairyness especially on the % of protein. With those prerogatives we wish you all to have a good start. Giovanni Binotti Sire Analyst COFA

New Bulls

ISID

OR

O

IT05

9902

2094

9 V

G 8

5 Th

e co

mpl

ete

staf

f of t

he C

apra

relli

farm

deliv year days Milk F % P %

1 03-00 270 3854 9,52 4,80

2 04-01 270 5872 7,48 4,73 Production of the buffalo az 427 IT059990136247

These excellent result are possible do to the tight cooperation with their consultant Dr. Giuseppe Ronci, with which they had planned, a few year earlier, a specific program where the goal was to upgrade the genetic of the herd. In no time the result that they were expecting came through, and the Caprarelli farm was able, with the cooperation of GB Genetics, to put into the COFA A.I. stud, to start a progeny test program, a young bull with great genetic characteristics, ISIDORO. This young sire is scored VG 85 at 2 years, is a sun of Allblack out of a Zerbio’s daughter with a Ciripicchio grand dame, a pedigree that will guarantee animals with great structure, high productivity and correct and strong feet & legs.

PKM +144,48,23 Milk +644,20 F Kg +42,08 F % -0,29 P Kg +27,65 P % +0,08

101

101

101

101

101

101

101

101

101

101

101

101

101

101

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Data ANASB Setpember 2015

Sire MALANDRINO III IT054CR003C174 VG 88,5

ALLBLACK IT077990045507 PKM 169,10 Data ANASB 2015

PKM 42,28 Data ANASB 2015 River ILENYA IT059990056285

n° year days Milk F% P% 3 03.07 270 5361 8,3 4,6

Dam PKM 173,90 Sept 2015 ZERBIO IT003990022938

Az 428 IT059990136387 VG 86 PKM 204,40 Data ANASB 2015

n° year days Milk F% P% Az. 184 IT059000222006 (Ciripicchio)

1 03.00 270 3615 9,8 4,8 2 04.01 270 3810 8,7 4,9

n° year days Milk F% P% 4 09.08 270 3140 8,3 4,9 Foto 20-09-2006

ZER

BIO

Ow

ner :

Fac

chi F

.lli

ALL

BLAC

K O

wne

r : B

rada

no R

iver

Data ANASB PKM +129,22 Milk +554,30 F Kg +39,48 F % -0,10 P Kg +24,00 P % -0,05

ISIDORO although it doesn’t have an Expected Morphology Index, is a sire that has the possibility to pass on to is progeny good and strong morphology treats in terms of structure, dairyness, udder composite and feet & legs. You can deduct this by looking at its pedigree, because we can find sire of the weight of Malandrino III and Zerbio that can give us good hope on the passing on of good jeans to is progeny. Isidoro is a bull that was score VG85 at the age of 2 years and its mother was score VG86 on second lactation. Because of its characteristics we don’t advise it on heifers..

ISIDORO new bull in the progeny test program, strong on structure and dairyness, it was scored VG 85

at 2 years.

+F

New Bull

Foto 22-10-2009

Milk +728,60 F Kg +48,00 F % -0,12 P Kg +31,00 P % -0,03

Data ANASB September 2015

Milk +745,75 F Kg +50,45 F % -0,21 P Kg +31,30 P % -0,08

Data ANASB September 2015 PASSATORE’s Sire

Sire IT054CR003C174 MALANDRINO CE 24552

MALANDRINO III

PKM 169,10 Data ANASB 2015

RESICA CR 1574 VG 86

n° year days Milk F% P% 3 04.06 270 51,18 7,6 4,3

Dam PKM 163,10 Sept 2015 AZ 3/95 ITLT000010377 EX 90,5

Az.1063 IT059990018965 VG 84

n° year days Milk F% P% AZ 336 ITLT000008380 G+ 83

1 02.07 270 3039 8,3 4,5 2 03.08 268 3655 8,6 4,6 3 04.07 241 3208 8,1 4,5 4 05.08 270 4173 7,6 4,4 5 06.10 270 3977 7,9 4,6

n° year days Milk F% P% 3 05.03 270 4329 8,0 4,5

PASSATORE exceptional Malandrino’s III son waiting for its proof.

Milk +442,35 F Kg +23,43 F % -0,29 P Kg +21,15 P % +0,02

Milk +676,50 F Kg +31,30 F % -0,54 P Kg +32,60 P % +0,06

CAESAR

Sire VG NAPOLI CE 28216

CAESAR IT019500148631

PKM 51,60 Data ANASB 2015 INSEGNA CR 630 G+ 82

n° year days Milk F% P% 6 08.02 270 4161 8,0 4,6

Dam PKM 147,20 Sept 2015 IT051CR035C004 VG 88,5

FIULA IT019990236392

n° year days Milk F% P% BAGOLA IT019500131508

1 02.07 270 3010 8,0 4,3 2 04.05 270 3833 7,6 5,0 3 05.09 270 4515 7,8 5,1 4 07.02 270 5209 8,4 4,7

n° year days Milk F% P% 3 05.02 258 2596 7,9 4,9

Data ANASB September 2015 Data ANASB September 2015

MALANDRINO III°

PKM +152,95 Milk +615,45 F Kg +46,75 F % -0,09 P Kg +28,70 P % +0,00

102

90

102

100

98

108

111

102

111

99

106

103

111

103

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Data ANASB September 2015

Sire VG 85 UNICO LT 6169 VG 89

GOKU IT019500148587

PKM 136,80 Data ANASB 2015 MAESTRA ITCR000000866 G+ 84

3 1997 270 4531 8,0 5,0

Dam PKM 179,60 Sept 2015 Malandrino III IT054CR003C174 VG 87

Rver JANA IT077990042179 G+ 82 PKM 169,10 Data ANASB 2015

n° year days Milk F% P% River IT026990190977

1 02.08 260 3364 7,9 4,7 2 03.08 270 4519 8,2 5,0 3 05.01 270 3705 7,9 4,8

n° year days Milk F% P%

Foto 20-09-2006

MAL

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GO

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Data ANASB Dicember 2015 PKM +158,20 Milk +598,10 F Kg +45,95 F % +0,05 P Kg +30,25 P % +0,06

DIRECTION is a son of Goku on a Malandrino III daughters, his expected morphology index highlights his excellent traits especially the structure, production capability very good Feet & Legs and Fetterlock Strength. Direction is a bull that has to be protected on the mammary system, that means that he has to be bred with buffalos that have good Front Udder Attachment and Teats well placed. Direction goes well with daughter of: O-B-One, Caesar, Lucky Jece Sole and Zerbio.

DIRECTION new bull in A.I. with the Sire and Maternal Grand Sire that has the Production and

Morphology Indexes.

New Bull

PKM +127,40 Milk +521,65 F Kg +36,55 F % -0,23 P Kg +24,75 P % +0,01

103

100

90

108

96

106

98

103

94

104

105

110

104

106

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Data ANASB Septembree2015

Foto 20-09-2006

GO

KU O

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ri F.

lli

Data ANASB Dicember 2015 PKM +107,30 Milk +496,85 F Kg +30,80 F % -0,30 P Kg +20,90 P % -0,06

AGRIPPA is a new A.I. progeny bull that has very good expected morphology indexes, you can see that from the graph that brings to attention the fact that it improves the stature, the dairyness, feet & legs and the fetterlock straight. Very goon on the rear udder attachment. It should be used on buffalo that have good stature and a high udder position compare to the distance to the hock. A sure promise for the Italian selection.

AGRIPPA is a son of Jesce Sole on a Goku’s dam, a very interesting breed for its production and

dairyness potential.

+F

Sire VG 85 ITCE000055811

JESCE SOLE IT061000057361

PKM 118,00 Dati ANASB 2015

IT061000023393 G+ 80 86

n° year days Milk F% P% 5 08.06 270 3566 9,6 4,9

Dam PKM 96,60 Sept 2015 GOKU IT019500148587

Az.895 IT059990097279 G+ 83

n° year days Milk F% P% AZ 1051 IT059000237698 G+ 82

1 02.00 270 2797 7,3 4,4 2 03.06 268 3624 7,9 4,5 3 04.10 250 3656 8,4 4,5 4 05.10 242 2314 8,7 4,5 5 06.10 231 2022 8,7 5,0

n° year days Milk F% P% 3 06.02 270 3337 8,3 4,5

JESC

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F.lli

Cas

erta

New Bull

PKM Milk F Kg F % P Kg P %

101

101

101

101

101

101

101

101

101

101

101

101

101

101

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Data ANASB September 2015

Foto 20-09-2006

MAL

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Data ANASB PKM +148,70 Milk +672,60 F Kg +46,40 F % -0,16 P Kg +27,40 P % -0,10

APPIO is a new A.I. progeny bull that is impressive in stature, body length and chest high. Very strong on the back line and for its strong dairyness. Appio’s mother is an excellent buffalo scored G+83 on first lactation. Very good is her production trait in the genetic index +582,30 and exceptionally strong on the % of protein +0.06.

+F

Sire IT054CR003C174 MALANDRINO CE 24552

MALANDRINO III

PKM 169,10 Data ANASB 2015 RESICA CR 1574 VG 86

n° year days Milk F% P% 3 04.06 270 51,18 7,6 4,3

Dam PKM 128,20 Sept 2015 AZ 10/06 IT059990064982

Az.3356 IT059990175801 G+ 83

n° year days Milk F% P% AZ 221 IT059000211637 G+ 81

1 02.08 270 2870 8,0 4,7 2 03.08 270 3570 8,2 4,7

n° year days Milk F% P% 2 04.09 270 4043 8,3 4,5

PKM +128,20 Milk +582,30 F Kg +39,90 F % -0,01 P Kg +23,20 P % +0,06

Data ANASB September 2015

New Bull

Sire VG 85 ITCE000055811

JESCE SOLE IT061000057361

PKM 118,0 Milk 575,3 Sept. 2015 IT061000023393 G+ 80

n° year days Milk F% P% 5 08.06 270 3566 9,6 4,9

Dam PKM 137,00 Sept 2014 ITLT000006997 EX 93

AZ 935 IT059000107917 G 79

n° year days Milk F% P% AZ 119 ITLT000006715 G+ 80

1 02.05 270 3071 8,0 4,6 2 03.08 270 3810 7,4 4,7 3 04.10 266 3380 8,5 4,7 4 05.10 270 3564 8,4 4,7 5 06.10 245 2999 8,1 4,6 6 07.10 270 2814 7,7 4,7 7 09.01 270 3534 8,1 4,7

n° year days Milk F% P% 4 06.00 270 4267 8,1 4,6

Pictured 20-09-2006

Sire 11/96 ITLT000013046 VG 88,5

7/06 IT059990064965 VG 86

AZ 613 IT059000058777 G+ 84

n° year days Milk F% P% 4 05.11 237 3405 8,1 4,6

Dam PKM 177,00 Sept 2015 3/95 ITLT000010377 EX 90,5

AZ 1169 IT059990037772 G+ 80

n° year days Milk F% P% AZ 345 IT059000024181 G+ 82

1 02.10 270 3866 8,9 4,6 2 04.00 270 3742 7,9 4,7 3 05.02 229 3713 7,0 4,3 4 06.02 270 3847 7,7 4,5 5 07.04 270 4117 8,3 4,4

n° year days Milk F% P% 5 07.07 270 3101 8,9 4,6

JAFAR da Izano IT054CR003C167

Sire VG 85 ITCE000055811

JESCE SOLE IT061000057361

PKM 118,0 Milk 575,3 Sept 2015 IT061000023393 G+ 80

n° year days Milk F% P% 5 08.06 270 3566 9,6 4,9

Dam PKM 146,60 Sept 2015 ITLT000011712 VG 89,5

AZ 1002 IT059000237648 G+ 83

n° year days Milk F% P% AZ 898 IT059000107895 G+ 81

1 02.11 270 3829 8,4 4,6 2 04.05 237 3907 9,0 4,7 3 05.05 230 3666 8,4 4,6 4 06.06 270 3532 7,0 4,4 5 07.10 270 3485 7,7 4,4

n° year days Milk F% P% 2 04.01 250 2470 8,7 4,7

+F

+F

+F

PKM +88,50 Milk +413,60 F Kg +25,90 F % -0,08 P Kg +16,09 P % -0,03

Data ANASB September 2015

PKM +132,30 Milk +618,90 F Kg +37,15 F % -0,31 P Kg +25,95 P % -0,03

Data ANASB September 2015

PKM +129,20 Milk +590,81 F Kg +35,02 F % -0,39 P Kg +25,88 P % -0,05

Data ANASB September 2014

Mastro Titta is an excellent new A.I. progeny bull that deep roots in the Circe farm. The father is a farm bull was scored VG 86, a bull with great stature and very correct on all of is functional traits. The mother, Az.1169, is a buffalo with big productions, on her 5th lactation she had Kg 4117 of milk in 270 days, she’s 32nd on the September 2014 genetic index with a PKM of 184,20 and +853 in milk. Mastro Titta is scored G+84, it has great strength on the back line excellent rump and an extraordinary dairyness capability.

Musolino is a son of Jesce Sole a bull that doesn’t need any comment. The mother, Az 1002, is a buffalo with very good productions scored G+83, present on the September 2014 genetic Index on the 80th place with a PKM of 157,10 and +701,16 on milk with good fat and protein. The maternal grand sire was scored VG 89,5 a lot of its daughters that are present in the farm distinguish themselves for their productions. Musolino is scored G +82 with very good feet & legs trait and a good structure and very good dairyness.

Papa Ciro is a son of Jesce Sole, the mother, Az936, is a buffalo with very good productions and very regular on the delivery. The maternal dam, Az119, is a fantastic buffalo that had a production of Kg 4267 of milk with 8,1% fat and 4,6% protein. The maternal grand sire, LT6997, is an extraordinary bull scored Ex93, one among the best bulls ever scored in the selection of the Mediterranean Italian Buffalo. Papa Ciro is scored G+83, a bull well balance on all of is morphology trait and especially good on feet & legs.

Expected Genetic Index

Expected Genetic Index

Expected Genetic Index

PKM +110,68 Milk +424,73 F Kg +33,53 F % -0,02 P Kg +20,08 P % +0,03

102

90

102

100

98

108

111

102

111

99

106

103

111

103

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Data ANASB September 2015

Sire VG 85 UNICO LT 6169 VG 89

GOKU IT019500148587

PKM 136,80 Data ANASB 2015 MAESTRA ITCR000000866 G+ 84

n° year days Milk F% P% 3 1997 270 4531 8,0 5,0

Dam PKM 255,40 Set 2015 Malandrino III IT054CR003C174 VG 88

UDEA IT003990003934 VG 86 PKM 169,10 Data ANASB 2015

n° year days Milk F% P% OLIMPIA IT003108000786 G+ 80

1 02.03 270 4880 7,8 4,8 2 03.10 270 5094 7,2 4,6 3 05.01 270 4637 8,2 4,4 4 06.06 270 4627 8,2 4,4 5 07.08 270 3627 7,8 4,3

n° year days Milk F% P% 1 02.04 270 3959 8,7 4,8 Foto 20-09-2006

MAL

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Data ANASB Dicember 2015 PKM +196,10 Milk +836,60 F Kg +55,00 F % -0,14 P Kg +38,45 P % +0,04

GOKHAN is new progeny bull that, as you may see from its expected Morphology Index, has great traits on structure, stature and length of the body. Excellent feet & legs and fetterlock strength. On the udder side it’s excellent its rear attachment. It has slight negativity on the length of front udder attachment and on the direction of the teats. Gokhan is a bull to be used on buffalo with good udder composite.

GOKHAN new A.I. progeny bull with sire and maternal grand sire with Production and

Morphology indexes

New Bull

PKM Milk F Kg F % P Kg P %

Data ANASB September 2015

Sire VG 85 ITCE000055811

JESCE SOLE IT061000057361

PKM 118,00 Data ANASB 2015 IT061000023393 G+ 80

5 08.06 270 3566 9,6 4,9

Dam PKM 215,40 Sept 2015

River ALIDA IT059990056213 G+ 84

n° year days Milk F% P% IT059990000817

1 02.06 270 2722 9,9 5,7 2 03.09 270 4062 9,1 5,5 3 04.11 270 4858 8,6 5,7 4 06.00 270 5110 8,6 5,3 5 07.03 270 4438 8,9 5,5

Foto 20-09-2006

JESC

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F.lli

Cas

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PKM +166,70 Milk +600,10 F Kg +38,35 F % -0,14 P Kg +38,50 P % +0,13

5,205,305,405,505,605,705,805,906,006,106,20

30 60 90 120 150 180 210 240 270

1° Lactation2° Lactation3° Lactation

Days in Lactation

% o

f Pro

tein

PERCENTAGE OF PROTEIN PRODUCES IN 3 DIFFERENT

LACTATIONS

0,02,55,07,5

10,012,515,017,520,022,525,0

30 60 90 120 150 180 210 240 270

1° Lactation2° Lactation3° Lactation

Lite

rs o

f milk

pro

duce

d pe

r day

Days in Lactation

MILK PRODUCES IN 3 DIFFERENT LACTATIONS

PKM 215,40 Milk +624,90 F Kg +52,40 F % +0,38 P Kg +44,20 P % +0,38

River ALIDA is the # 1 for % of Protein.

ALIDA is a fantastic buffalo of the Bradano River farm that combines with her tremendous production extraordinary percentages of fat and protein. ALIDA is the best buffalo in Italy on the index for protein % with her +0,38%.

Data ANASB September 2015

RAINBOW a young progeny bulls that promises excellent milk production with great Protein.

+F

ISIDORO BLACKSTAR Our selection objectives, for what concerns the morphology side, are concentrated on those sire that have the capability to transfer on their daughters great stature with excellent udder composites and correct feet & legs. In order to do this we have insert in our team sons of Malandrino III like BLACKSTAR, PASSATORE, ALLBLACK and APPIO, this is to guarantee animals with great stature. We also insert sons of O-B-One like CICLOPE, BATIMAN and CARLO II, in order to obtain good result on udder indexes, in a near future we are thinking in getting sons of BASULU’ and OMBRA e NISCIUNO, to guarantee us excellent udder composite with good conformation and direction of the teats, the udder has a weight of 40% on the evaluation of a buffalo that is why these traits are a priority and fundamental to have in a buffalo. To improve feet & legs we are putting our trust on sire like LUCKY, CAESAR, ZERBIO, BLACKSTAR and BASULU’. All those characteristics are necessary in order to get animals that are productive, functional and with good longevity, all traits necessary in order to help the farmers in getting well balance animals that helps them to get a positive financial balance at the end.

MORPHOLOGY PRODUCTION

When we select sire for the future generation we ask ourselves two questions: 1 - Farmer’s needs. 2 - Use of the milk. Concerning point 1 is useless to say that the quantity of milk is the first priority for a farmer, that is why we select in the past sire that could guarantee high productions like the sons of Malandrino III, Jesce Sole, now we are looking at the new generation with sons of GOKU, OMBRA e NISCIUNO and in particularly ZERBIO that with is 129 indexed daughters is for sure the n°1. Beside the quantity of milk we are looking also at the quality of it in terms of % of fat and protein, very important factors when you transforms it into cheese and a sire that will definitely help us will be GOKU, that is why we’re trying to have it either in the paternal line than in the maternal one. Concerning point 2 is well known that 90% of the milk, in Italy, is used for the production of mozzarella cheese and other semi aged type of cheeses. By keeping this in mind it explains why it’s a must that we need a sire that has high and positive trait on fat and protein. This is the reason why we use young sire with, on their blood line the sire GOKU, that for sure is the best Tested Bull that can unite to an excellent production an extraordinary quality of the milk in terms of % of fat and protein. It goes without saying that in order to have a good buffalo we can’t just look at production but we have to look also at other morphology trait that can guarantee us a good food intake with good transformation and longevity, so we may end by saying that production and longevity have a close correlation with functionality.

We are working very hard in order to be able to make available for the

farmers the best genetic

Pretty soon we’ll have available sons of: ZERBIO

OMBRA e NISCIUNO BASULU ‘

82 99

87 101

96

82 97

86 111

101

89 99

80 92 104 116 128

GOKU da Izano IT019500148587

Sire VG 85 SA 69871

IT065000261262

CAMERIERA SA 59489

4 06.01 270 3398 8,5 5,0

Dam SA 50780 VG 86

MATTINIERE IT099SA001C035

n° year days Milk F% P% TROFEO SA 63119

1 02.09 270 3029 8,2 4,7 2 03.10 254 3078 7,8 4,8 3 04.11 238 3457 8,9 4,8 4 05.11 220 3199 7,8 4,9 5 06.10 260 3519 8,4 4,8 6 07.11 236 3032 8,2 4,3

n° year days Milk F% P% 4 07.00 270 3943 9,7 5,4

Pictured 20-09-2006

Data ANASB September 2015

Sire VG 89 LT 642 VG 85

UNICO LT 6169

PKM 1,21 Dati ANASB 2010

2 270 3191 7,4

Dam MALANDRINO CE 25552

MAESTRA CR 866 G+ 84

n° year days Milk F% P% AZ. 32 CE 24579 G+ 81

1 1995 270 3118 8,8 4,9 2 1996 270 3672 8,0 4,9 3 1997 270 4531 8,0 5,0 4 1999 270 4563 8,1 4,7

n° year days Milk F% P% 3 1998 270 3412 8,1 4,5

95

88 82 82

105 99

105 95

83 104

109 108

95

80 92 104 116 128

PKM +136,80 Milk +468,00 F Kg +40,60 F % +0,12 P Kg +25,80 P % +0,13

Daug. 150 Herds 46

Daug. 212 Lact. 571 Herds 56 98% R

Daug.173 Lact. 560 Herds 39 98% R

PKM +46,70 Milk +162,50 F Kg +10,60 F % -0,03 P Kg +10,00 P % +0,09

Daug. 86 Lact. 227 Herds 26 96% R

JAFAR da Izano IT054CR003C167

Sire MARCUCCIO SA 28412

ALDESTE SA 42196

DATA SA 31116

3 270 4575 8,7

Dam MALANDRINO CE 25552

INSEGNA CR 630 G+ 82

n° year days Milk F% P% COLORIZIO CE 28245

1 1995 270 2713 9,7 4,7 2 1996 270 3726 8,0 4,5 3 1997 263 3664 7,7 4,7 4 1999 270 3944 8,1 4,7 5 2000 270 3849 7,8 4,6 6 2001 270 4161 8,0 4,6

n° year days Milk F% P% 6 1998 270 4379 7,2 4,0

114 107

102 109

88 107

105 100

123 117

95 106

116

80 92 104 116 128

GOKU’s Daughter GOKU’s Dam

Mother of JAFAR

MAESTRA

INSEGNA

MATTINIERE

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Daug. 121 Herds 36 General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

PKM +45,20 Milk +163,40 F Kg +16,40 F % +0,13 P Kg +7,40 P % -0,01

Data ANASB September 2015

Data ANASB September 2015

Mother of

SPARTACUS

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Daug. 63 Herds 23

GOKU da Izano IT019500148587

Nato il 02-01-2005: Morese Giuseppe

Pontecagnano Salerno Italy

Sire VG 85 SA 69871

IT065000261262

CAMERIERA SA 59489

n° year days Milk F% P% 4 06.01 270 3398 8,5 5,0

Dam SA 50780 VG 86

MATTINIERE IT099SA001C035

n° year days Milk F% P% TROFEO SA 63119

1 02.09 270 3029 8,2 4,7 2 03.10 254 3078 7,8 4,8 3 04.11 238 3457 8,9 4,8 4 05.11 220 3199 7,8 4,9 5 06.10 260 3519 8,4 4,8 6 07.11 236 3032 8,2 4,3

n° year days Milk F% P% 4 07.00 270 3943 9,7 5,4

Pictured 20-09-2006

Data ANASB September 2015

PKM +84,55 Milk +381,45 F Kg +26,45 F % -0,15 P Kg +15,80 P % -0,07

PKM +28,60 Milk +97,20 F Kg +10,40 F % +0,13 P Kg +4,60 P % -0,01

Daug. 77 Lact. 214 Herds 28 96% R

JAFAR da Izano IT054CR003C167

PKM +138,00 Milk +841,15 F Kg +48,81 F % -0,16 P Kg +30,13 P % -0,12

Data ANASB September 2010

Data ANASB September 2015

Sire IT054CR003C174 MALANDRINO CE 25552

MALANDRINO III

RESICA CR 1574 VG 86

n° year days Milk F% P% 3 04.06 270 5118 7,6 4,3

Dam

River ILENYA T059990056285

n° year days Milk F% P%

1 02.05 270 3523 8,5 4,9 2 03.07 270 5361 8,3 4,6

PKM 169,10 Data ANASB 2015

Sire GECK SA 38324

CONDOR VA 277 VG 88

Az 3054 SA 36066 G+ 82

5 08.02 255 3908 8,2 4,2

Dam MALANDRINO III

TERZA IT003900021248

n° year days Milk F% P% OTTAVIA IT003108000842

1 02.07 270 4623 7,2 4,3 2 04.09 270 6460 8,4 4,5

n° year days Milk F% P% 2 03.11 270 4069 7,4 4,4

MALANDRINO III

TERZA

TERZA Mother of BASULU’ Az. Agr. Facchi F.lli Novara

MALANDRINO III° Father of ALLBLACK Az. Agr. Massari F.lli Cremona

Milk +1.682,30 F Kg +97,61 F % -0,32 P Kg +60,26 P % -0,25

Data ANASB

September 2010

97

102

99

104

81

113

130

115

93

110

91

117

101

80 92 104 116 128

General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Daug. 53 Herds 22

ETTORE Champion at the 2013 national fair in Naples

List 1

List1: Traits considered by ANASB on the morphology index.

List 2: Hereditability of the considered traits..

Source ANASB

List 2

HEREDITABILITY As you can see on List 2 the hereditability of the traits varies a lot, we can say that the higher the hereditability the faster are the genetic changes of that traits. Low hereditability correspond to a slower genetic improvement. We can see that the traits DC, FOPA, LAAM and ARTP are traits with low hereditability and you can see this on the buffalo population because a lot of animals have those negative characteristics. On the contrary STAT, PG and AM are traits that improve quicker. It’s advisable then to concentrate more on the traits with low hereditability.

COMPOUNDS TRAITS These are the traits that on a morphology evaluation of a buffalo are grouped together, with different weigh in order to be able to score the four traits listed below. • SRT is the evaluation of all of the traits that

identify the structure of a buffalo. • ARTP is the evaluation of all of the traits of the

feet and legs of a buffalo. • PP is the evaluation of all of the traits that

influence the production capacity of a buffalo. • AM is the evaluation of all of the traits of the

mammary system of a buffalo. Evaluated those four traits you use them to create the final evaluation of the animal • PG is the general or final evaluation of the

animal that contains with different waits (List 3), all of the compounds traits.

The result of all these evaluations it helps us understand how close the animal is to our ideal model. Giovanni Binotti Sire Analyst COFA

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STR ARTP PP AM PG

Structure Feet & Legs Dairyness Udder Composite General Score

List 3 Source ANASB

% 15 25 20 40

WAITS OF THE COMPOUNDS TRAITS

THE IDEAL BUFFALO In the criteria of the selection o f any productive animals a big and important aspect is taken by the functional morphology, this because of the productivity that an animal can give to the farmers, that is why we try with genetics improvement to get as close as possible to the ideal model. Talking especially about buffalos it is clear that the ideal buffalo has to be an animal with high milk productions and in order to do so it has to have a morphology that will allow it to intake and transform a big quantity of food, therefore are very important the traits in term of Stature, Transversal Diameters and Longitudinal Diameters. What we have said up to now is not sufficient, a buffalo to be productive it has to stay on the farm as long as possible that is why Feet & Legs are very important, a buffalo with big lacks won’t be productive and will not stay long on the farm. All of this is still not sufficient, if we consider that we are selecting an animal that has to produce milk, the Udder Composite is fundamental and for sure on the first place on our list. Udder with defect causes problems in milking, have tendency to have mastitis and inevitably animals with little longevity and productivity that will leave soon the herd.

PG STR ARTP PP AM STAT AT LT FOPA LAAM LAPM DPG DC LC

MORPHOLOGY TRAITS General Score Structure Feet & Legs Dairyness Udder Composite Stature Chest High Body Length Fetterlock Strength Front Udder Attachment Length Rear Udder Attachment With Distance to the Hock Teats Direction Teats Length

Hereditability h*

0,33 0,25 0,19 0,20 0,30 0,39 0,23 0,20 0,17 0,18 0,24 0,27 0,15 0,21

F G

nRH

PGF 2cc

GnR

H

A.I.

CL Day 0: 1 shot of GnRH Day 7: 1 shot of prostaglandine F2a Day 9: 1 shot of GnRH 48 hours after PGF2a Day 10: Inseminate 16 hours after day 9 GnRH shot

CL Regression

0 days 7 days 48 hours 16 hours

28 ± 4h ovulation

Ovulation or CL formation

Diagram of ovarian dynamics and synchronization response for traditional OvSynch hormone injection schedule

F=follicle; CL=corpus luteum

Ovsynch protocol in buffalo

day 28 day 35

Buffaloes closed

PGF2a to open buffaloes

day 37

GnRH + TAI (16 h) later GnRH to all Buffaloes

Ultrasaund

TAI

day 0

Resynch protocol in buffalo

Prosynch protocol in buffalo heifers

GnR

H

GnR

H

PGF 2cc

PGF 2cc

GnR

H

A.I.

Diagram of ovarian dynamics and synchronization response for Prosynch Protocol hormone injection schedule in Buffaloes heifers

Ovulation or CL formation

CL from earlier GnRH Plus CL formed from this GnRH

Ovulation or CL formation

F CL CL CL CL CL CL F

Regression Regression

0 days 3 days 8 days 7 days 48 hours 16 hours

Pict. 1

Pict. 2

Pict. 3

Ovsynch pict. 2 Is the appropriate protocol for ovulation induction for the buffalos in lactation.

Resynch pict. 3 ( INSEMINATION PRESSING ) Is the appropriate protocol in order to reduce the synchronization times of the unpregment buffalos and to strengthen the pregnant buffalos.

Prosynch pict. 1 Is the appropriate protocol for synchronization of the ovulation for the buffalo heifers

In order to obtain great result in conception rate, you don’t only need good herd management with a correct ration and a good technic in artificial insemination, you also need to have great quality semen material. That is why COFA, on this matter has always put special attention, and has come up with specific protocol for the semen buffalo production. At COFA, we don’t only look at the motility and concentration of the semen, but we pay very good attention at the morphology evaluation of the sperm, that allows us to make and estimate of the fertility, not only in theory at the lab but also on hand in the field.

Another very important aspect, in order to be able to produce high semen quality, is the freezing, a very delicate process. That is why over the years we have created and perfected a SPECIAL curve only for the buffalo semen, with great result.

During the year 2014 we had 14 buffalo in production, and we had an excellent result on the quality of the semen on all of them like it shown on the table below: PROGRESSIVE MOTILITY 57,0% TOTAL CONCENTRATION 18,1 millions NPMS (Number of Progressive Motile Sperm) 10,3 millions MORFOLOGY anomalies less than 20%

With this result COFA puts itself as the world leading company in the production of high quality buffalo semen material. In the semen we make, you won’t only find and get high genetics, but you’ll also get great sperm quality. We can say this because of the feedback we get from or numerous client and veterinarian that operates in the field in Italy and all over the world. Cinzia Pozzaglio COFA Laboratory Technician

Via Orezola snc 26048 Sospiro

Cremona Italy

Warehouse of the A.I. Stud Benedetta Bischeri

Ph. : (+39) 0372.621248 Fax: (+39) 0372.621519

e-mail: [email protected] Web site: www.cofa-it.com

Laboratory of the A.I. Stud

Cinzia Pozzaglio Ph. : (+39) 0372.621248 Fax: (+39) 0372.621519

e-mail: [email protected]

Foreign Market Mattia Casali

e-mail: [email protected] Skype: mattia1465

Cell.: (+39) 335.6155915

Accounting Maddalena Maiocchi

e-mail: [email protected] Ph.: (+39) 0372.28842 Fax: (+39) 0372.26106

Italian Market

Giovanni Binotti G.B. GENETICS

e-mail: [email protected] Cell.: (+39) 338.5827257

Az. Ag. Facchi f.lli – Heifers Barn

Bradano River ZEUDI IT059990072579 2° lact. 299 days Milk Kg 4.727 F % 8,42 P % 4,96 BEST UDDER at the National Fair in Foggia in 2010