isolation and characterization of magnetic bacteria from ... · this powerpoint 2007 template...
TRANSCRIPT
RESEARCH POSTER PRESENTATION DESIGN © 2015
www.PosterPresentations.com
(—THIS SIDEBAR DOES NOT PRINT—)
DES IG N G U IDE
This PowerPoint 2007 template produces a
36”x48” presentation poster. You can use it
to create your research poster and save
valuable time placing titles, subtitles, text,
and graphics.
We provide a series of online tutorials that
will guide you through the poster design
process and answer your poster production
questions. To view our template tutorials, go
online to PosterPresentations.com and click
on HELP DESK.
When you are ready to print your poster, go
online to PosterPresentations.com
Need assistance? Call us at 1.510.649.3001
QU ICK START
Zoom in and out As you work on your poster zoom in
and out to the level that is more
comfortable to you.
Go to VIEW > ZOOM.
Title, Authors, and Affiliations Start designing your poster by adding the title, the
names of the authors, and the affiliated institutions.
You can type or paste text into the provided boxes.
The template will automatically adjust the size of
your text to fit the title box. You can manually
override this feature and change the size of your
text.
TIP: The font size of your title should be bigger than
your name(s) and institution name(s).
Adding Logos / Seals Most often, logos are added on each side of the title.
You can insert a logo by dragging and dropping it
from your desktop, copy and paste or by going to
INSERT > PICTURES. Logos taken from web sites are
likely to be low quality when printed. Zoom it at
100% to see what the logo will look like on the final
poster and make any necessary adjustments.
TIP: See if your school’s logo is available on our
free poster templates page.
Photographs / Graphics You can add images by dragging and dropping from
your desktop, copy and paste, or by going to INSERT
> PICTURES. Resize images proportionally by holding
down the SHIFT key and dragging one of the corner
handles. For a professional-looking poster, do not
distort your images by enlarging them
disproportionally.
Image Quality Check Zoom in and look at your images at 100%
magnification. If they look good they will print well.
ORIGINAL
DISTORTED
Corner handles
Go
od
pri
nti
ng
qu
alit
y
Bad
pri
nti
ng
qu
alit
y
QU ICK START ( con t . )
How to change the template color
theme You can easily change the color theme of your poster
by going to the DESIGN menu, click on COLORS, and
choose the color theme of your choice. You can also
create your own color theme.
You can also manually change the color of your
background by going to VIEW > SLIDE MASTER. After
you finish working on the master be sure to go to
VIEW > NORMAL to continue working on your poster.
How to add Text The template comes with a
number of pre-formatted
placeholders for headers and
text blocks. You can add
more blocks by copying and
pasting the existing ones or
by adding a text box from the
HOME menu.
Text size Adjust the size of your text based on how much
content you have to present. The default template
text offers a good starting point. Follow the
conference requirements.
How to add Tables To add a table from scratch go to the
INSERT menu and
click on TABLE. A drop-down box will
help you select rows and columns.
You can also copy and a paste a table from Word or
another PowerPoint document. A pasted table may
need to be re-formatted by RIGHT-CLICK > FORMAT
SHAPE, TEXT BOX, Margins.
Graphs / Charts You can simply copy and paste charts and graphs
from Excel or Word. Some reformatting may be
required depending on how the original document
has been created.
How to change the column
configuration RIGHT-CLICK on the poster background and select
LAYOUT to see the column options available for this
template. The poster columns can also be
customized on the Master. VIEW > MASTER.
How to remove the info bars If you are working in PowerPoint for Windows and
have finished your poster, save as PDF and the bars
will not be included. You can also delete them by
going to VIEW > MASTER. On the Mac adjust the Page-
Setup to match the Page-Setup in PowerPoint before
you create a PDF. You can also delete them from the
Slide Master.
Save your work Save your template as a PowerPoint document. For
printing, save as PowerPoint or “Print-quality” PDF.
Print your poster When you are ready to have your poster printed go
online to PosterPresentations.com and click on the
“Order Your Poster” button. Choose the poster type
the best suits your needs and submit your order. If
you submit a PowerPoint document you will be
receiving a PDF proof for your approval prior to
printing. If your order is placed and paid for before
noon, Pacific, Monday through Friday, your order will
ship out that same day. Next day, Second day, Third
day, and Free Ground services are offered. Go to
PosterPresentations.com for more information.
Student discounts are available on our Facebook
page.
Go to PosterPresentations.com and click on the
FB icon.
© 2015 PosterPresentations.com 2117 Fourth Street , Unit C Berkeley CA 94710
Magnetotactic bacteria (MTB) are
aquatic fastidious prokaryotes whose
direction of motility is directed by the
Earth's geomagnetic and externally applied
magnetic fields. This response is due
Nano-sized magnetic crystals inside the
cells. These crystals called magnetosomes
and known to consist two types of minerals
i.e., iron oxides magnetite (Fe3O4) and
iron sulfides greigite (Fe3S4). They vary in
morphology, size, composition as well as
arrangements; and a single cell may
synthesize one-multiple chains. MTB vary
in Shapes including bacillus, cocci and
multicellular forms adding diverse
ecological habitats ranging from aquatic
(marine and freshwaters), to deep sea
sediments. The highest numbers of MTB
are reported close to the oxic-anoxic
transition zone. In this work, collection
method was designed to isolate MTB from
different water environment sources in
Saudi Arabia.
Introduction
Objective
Sites of collection:
(Fig. 1) Schematic diagram
showing the samples were
collected from different water
environment sources in Saudi
Arabia.
Materials and methods
C- Transmission electron
microscope :
Result
Conclusion
The present study, the effort mainly
focused on isolation and characterization
of magnetotactic bacteria from different
water environment sources in Saudi Arabia
based on a combination of different
techniques that used by researchers around
the world and our personal experiments.
MTB were isolated from Hanifa
Valley sample in a mixed culture and their
circular magnetite magnetosomes
arrangement were observed under TEM as
singles, chains and clusters. Species was
not determined due to the time limitation,
difficulties in separation methods as well
as unavailability of selective media in the
place of this study.
Moreover, there are several great
applications for this group of bacteria in
nanotechnology such as use the
magnetosomes as a potential drug-carrier
for tumor treatment and as contrast
material for Magnetic resonance imaging
(MRI), which attract our attention to
complete research on this area in the near
future.
References
1-Jogler C, et al. 2009. Towards cloning
the magnetotactic metagenome:
identification of magnetosome island gene
clusters in uncultivated magnetotactic
bacteria from different aquatic sediments.
Appl. Environ. Microbiol. 75:3972–3979.
2- Lefèvre, C.T.,and Abreu, F., Lins, U.,
Bazylinskim, D.A. 2011.A bacterial
backbone: magnetosomes in magnetotactic
bacteria. Springer 15, 75-102.
3- Yan L, Zhang S, Chen P, Liu H, Yin H,
et al. (2012) Magnetotactic bacteria,
magnetosomes and their application.
Microbiol Res 167: 507-519.
Acknowledgement
The authors extend their appreciation to
the Deanship of Scientific Research at
King Saud University for funding this
work through the Undergraduate Research
Support Program, Project no. (URSP -3-
18-142).
We are thankful to the Botany and
Microbiology department, College of
Science at King Saud University for giving
us the opportunity to complete this
research.
We also would like to express our very
great appreciation to Dr. Abeer Hashem for
her valuable and constructive suggestions
during the planning and development of
this research work. With a special thanks to
Jeddah divers league for collecting and
shipping JED samples and Dr. Manal Al
khulifi for discussions and our supervisor
Roua Alkufeidy.
Isolation and characterization of
Magnetic Bacteria from different water
environment sources in Saudi Arabia.
Supervised by: Dr.Roua ALKufeidy
Isra Faisal Abuturbush – Raneem Saleh Aldouhan - Moneera Ibrahim AlSanea
[email protected] [email protected] [email protected]
Isolation And Characterization of Magnetic Bacteria from
different water environment sources in Saudi Arabia Deanship of Scientific Research
Enrichment and
observation
Incubation at room temperature
Isolation and Cultivation of MTB
Microscopic examination
Transmission electron microscopy
Energy dispersive x-ray
spectroscopy
Isolation and Enrichment of the samples from natural environment
A- Results of the different
cultures collection:
The following table lists site, type
and date of collection, incubation
period, enrichment and first hanging
drop result for each sample
Gram stained bacteria appeared in different shapes such as cocci and bacilli, even though all of them were gram positive and negative .
Bacteria were assembled and attracted to the edge of the hanging drop during magnet placement, under both of light and digital microscope.
1.*: The sample was excluded in the mentioned stage. **: The test was not performed
D- Energy dispersive x-ray
spectroscopy:
EDXS analyses have been used to evaluate single-element incorporations into magnetite crystals produced by MTB.
Transmission electron microscope (TEM) is the technique of choice for observing individual magnetosomes and to absolutely classify a bacterium as capable of producing magnetosomes.
We used both Light and digital microscope , MTB were found to be highly motile and refractile and exhibited clumping under the application of magnetic field.
The magnet is an effective tool that was used as a MTB trap
(several days to months ).
Nutrient agar and Luria-Bertani and Charcoal agar solid media were used for bacterial cultivation.
(Fig. 13) EDXS microanalysis of the grid (A)analysis revealed that Fe and O are present in the specimen of Hanifa Valley. (B)S, Fe and O are present in the specimen of Jeddah. (C)Fe and O are present in the specimen of Yanbu. (D)Fe does not exist in the specimen of AL-Khafji.
(Fig. 11) Transmission electron
micrograph of a thin-sectioned cell
of MTB showing growing magnetite
crystals within the vesicles of the
magnetosome membrane (MM).
(Fig. 12) Transmission electron micrograph of another thin-sectioned cell of MTB revealed the magneto particles to range between 10 to 20 nm in size presented in Hanifa Valley (freshwater) (E, K) ,Jeddah(F) and Yanbu(I) (Red Sea) All of them were coccoid. Negative result appeared in Al khafji city(H) (The Arabian Gulf sample ) .
B-Light and digital microscopes :
(Fig 2)
(Fig 3)
(Fig 4)
(Fig 5)
(Fig 6)
(Fig 8)
(Fig 9)
(Fig 10)
(Fig.
7G)
(Fig.
7D)
(Fig.
7A)
(Fig.
7B) (Fig.
7C)
(Fig.
7E)
(Fig.
7F)
(Fig.
7H)
(Fig.
7L)
(Fig.
7K) (Fig.
7J)
(Fig.
7I)
(Fig 6)
Hanging drop
method result
P18