isolating bacteria

7/25/2019 Isolating Bacteria

http://slidepdf.com/reader/full/isolating-bacteria 1/6

Isolating Bacteria

Prepared by:

Mohammad Yosry Himoony 20910850

li l!"assar 20911#2$

%baydah &ar %mar 2091109'

Mohammad ta(neh 20910#$2

)o*ember 2011

Page1

Micro. Lab.

Dr. Murad Ibraheem



Results:

+pecimen

, itshemolysis

Blood ger -hoclateger

Ma-on.yger

/+

E.Coli.Gamma

reysmall

Bro(n

largeood

gro(th

+malllact

ose

3erementin

g and pin.!*e gram

4arge

(hiteood

dro(th

MoraxellaCatarrhalis

.Gama

+mall and

bro(n

oodgro(thBro(n

)o gro(th!*e gram

ery littlegro(thsm

all and

(hite

Pseudomo

nas

aeruginos

aBeta

ood

gro(th+mall

rey and

large

4ittle

gro(th!*e gram

ery large

gro(th

and green

colonies

Salmonella

speciesGama

ood

gro(th

and grey

4arge and

grey

4arge

colonies

non

3erementin

g pale

colored6*e gram

ood

gro(th

small and

(hite

Shigella

speciesGama

ery good

gro(th

and smallrey

4arge and

grey

4arge andnon

3erementin

g pale6*e gram

ood

gro(th

smalla

and yello(

Page2



Staphyloc

occus

aureusBeta

4argerey

rey and

large

)o gro(th6*e gram

Yello( or

golden

Staphyloc

occus

epidermidi

s.Gama

7hite nd

small

+malla

and (hite

)o gro(th6*e gram

7hite and

good

gro(th

Streptococ

cus

pneumoni

alpha

yello(good

gro(th&iplococci

green andlittle

gro(th

)o gro(th6*e gram

ery little

gro(th

Streptococ

cus

pyogensBeta

reen and

some

gro(th

+mall and

(hite

)o gro(th6*e gram

ery small

and

(hite

littlegro(th

Strepyoco

ccus

!iridanslpha

+mall and

green

+mall and

(hite

)o gro(th6*e gram

Isolated

(hite and

*ery small4ittle

gro(th

CandidaGama

+mall and

(hite

+mall and

(hite

)o gro(th Yeast

+mall and

(hite

Page"



#iscussion:

1. $hat is the di%erence &et'een selecti!e and di%erentialmedia( Gi!e example o) each.

• &ierential media: sed to dierentiate bet(een gro(n

bacteria or ;<amples: Blood gar , Mac-on.ey 3or

gram negati*e bacteria

• +electi*e media: allo( gro(th 3or only certain bacteria and

inhibit gro(th o3 other 3orms e<amples inclde Mac-on.ey

3or lactose!3ermenter bacteria

2. $hich organism produces pin* colonies on MacCon*eyagar+ Explain(

Page,



• 4actose!3ermenter gram negati*e prodce pin. colonies

Becase it prodces lactic acid (hich lo(ers the pH o3 the

agar belo( #8 and react (ith agar reslting in appearance o3

pin. colonies

". -ou ha!e incu&ated some o) the plates in a candle ar+explain 'hy(

• /o pro*ide an optimm temperatre 3or bacterial gro(th and

then recogni=e its gro(th 3eatres

,. $hich is easier( /o isolate a pathogen )orm stool

specimen or )orm CS0 specimen 'hy(

• Isolating a pathogen 3rom -+ is mch easier than isolating it

3rom stool specimen becase stool is rich (ith normal >ora

. #iscuss di%erent patterns o) hemolysis on &lood agar($hat is producing hemolysis(

• /here are three patterns o3 hemolysis:1 lpha: partial hemolysis appears as greenish color2 Beta: -omplete hemolysis appears as clear =one o3hemolysis? ama: )o hemolysis

Page



• Hemolysis prodced by haemolytic en=ymes 3ond in some

bacteria

3. 0rom the &acteria you use 'hich one causes:

1 Pharyngitis: +treptococcs pyogens

2 @rinary tract in3ection: ; coli

? 7ond and brn in3ection: +taphylococcs ares

$ Bloody diarrhea: +almonella

5 bscess and s.in in3ections: +taphylococcs epidermis

# ;ndocarditis in I drg sers: +taphylococcs ares

' ;ndocarditis a3ter dental e<traction: +treptococcs *iridans

8 %ral or *aginal thrsh: -andida albicans

;)& % A;P%A/

Page3

isolating bacteria

Documents