investigating enzyme function. activity 11: investigating enzyme function limited license to modify....
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Activity 11
Investigating Enzyme Function
Activity 11: Investigating Enzyme Function
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Activity 11: Investigating Enzyme Function
Get Started
What are some of the specific things that enzymes do in cells?
What is unique about each protein’s structure that allows it to perform its function?
Activity 11: Investigating Enzyme Function
Introduction
An enzyme is a type of protein that speeds up, or catalyzes, a specific chemical reaction.
Read the introduction.
Activity 11: Investigating Enzyme Function
Introduction
Do you know anyone with lactose intolerance?
What do you know about the condition?
Activity 11: Investigating Enzyme Function
Introduction
Breakdown of lactose
Lactose + water glucose + galactose
Breakdown of glucose
Glucose carbon dioxide + water
lactase
yeast
Activity 11: Investigating Enzyme FunctionActivity 11: Investigating Enzyme Function
How do pH and temperature affect the function of the enzyme lactase?
Challenge
Activity 11: Investigating Enzyme FunctionActivity 11: Investigating Enzyme Function
Wear safety goggles at all times during this laboratory.
Keep your hands away from the hot plate, and wear insulated gloves or use potholders to move the beaker as you finish.
Know the safety procedures in case of fire. Do not allow solutions to touch your skin or
clothing. Clean up spills immediately. If accidental contact occurs, inform me, and rinse
exposed areas.
Safety Guidelines
Activity 11: Investigating Enzyme Function
Procedure
The lactose should not be mixed with the lactase until after the lactase has been heated.
Once the yeast is added to a test tube, fix a balloon over it as quickly as possible before placing the tube in a water bath.
You must work within the following parameters:
Activity 11: Investigating Enzyme Function
Procedure
Find a partner from another group and compare the results of your investigations.
Go back to your group, share the new information with your partner, then share it with the other pair in the group.
Informal Meeting of the Minds
Activity 11: Investigating Enzyme FunctionActivity 11: Investigating Enzyme Function
How Enzymes Work
Activity 11: Investigating Enzyme FunctionActivity 11: Investigating Enzyme Function
A. Does temperature affect the function of lactase? If yes, what is the effect? Explain, using evidence from the investigation.
B. Does pH affect the functioning of lactase? If yes, what is the effect? Explain, using evidence from the investigation.
Analysis 4
Activity 11: Investigating Enzyme Function
Follow Up
Most human cells function at a temperature of 37°C and a pH of around 7. From what you learned in this activity explain why it is important for humans to maintain temperature and pH homeostasis.
Analysis 6
Activity 11: Investigating Enzyme FunctionActivity 11: Investigating Enzyme Function
How do pH and temperature affect the function of the enzyme lactase?
Revisit the Challenge
Activity 11: Investigating Enzyme FunctionActivity 11: Investigating Enzyme Function
How Enzymes Work
Activity 11: Investigating Enzyme FunctionActivity 11: Investigating Enzyme Function
binding site catalyze enzyme lactase lactose pH protein
Key Vocabulary