introduction to the genomics sciences lab services and support
TRANSCRIPT
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Introduction to
The Genomics Sciences Lab Services and Support
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The GSL operates as a solution center and provides CHHE and the NCSU community, collaborators, and the scientific community with access to HTS, genotyping and support services
In addition to serving as a research resource, the GSL is committed to the education and training of CHHE students, faculty, and staff.
GSL General Mission
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GSL MISSION for CHHE
High-throughput sequencing technology at NCSU GSL will support CHHE researchers to conduct • Complete transcriptome analysis Transcriptome development Gene expression analysis)• ChIPseq• Gene mutation analysis• SNP analysis• DNA methylation • miRNA analysis• eQTL analysis• Genotyping by Sequencing • Genome wide association studies
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AIM 1. Provide expertise in study design and sample preparation. The Genomics Section will provide guidance in all areas of high-throughput sequencing services, including assistance with sample and project preparation, and implementation of protocols for sequencing on various platforms.
AIM 2. Provide high-throughput sequencing services. The Genomics Section will provide high-throughput sequencing technology and instrumentation, library preparation, and consultation for all levels of experimental design, development, and assessment.
AIM 3. Provide guidance and expertise on Big Data analysis. Provide guidance and expertise on Big Data analysis. The GSL is linked to NC State’s Bioinformatics Research Center (BRC) and large dataset management and analysis will be conducted at the BRC by coordinating with the STC Bioinformatician when needed.
GSL AIMS for CHHE
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Support from start to finish – AIMS 1-3
Experiment design
Experiment execution
Tissue collection
Nucleic acid extractionNucleic acid QC
Library synthesis
Sequencing
Data QC
Data analysis
Publication
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Experiment design
Experiment execution
Tissue collection
Nucleic acid extractionNucleic acid QC
Library synthesis
Sequencing
Data QC
Data analysis
Publication
Choosing the best platform(s), pooling and lane use
Watch and learn, FREE for CHHE - TruSeq, RiboZero, NebNext etc. Low input, Ultra low input
Support from start to finish – AIMS 1-3
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http://research.ncsu.edu/gsl
AIM 2
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AIM 2
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+ NextSeq500!!Coming soon!
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HiSeq
MiSeq
PacBio
These are cartoons of reads!!
Choosing the best platform – AIM 1
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HiSeq
MiSeq
PacBio
Choosing the best platform – AIM 1
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HiSeq
MiSeq
PacBio
Error typeLow complexity/diversity
AccuracyStarting quantity
Choosing the best platform – AIM 1
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Sanger Sequencing
Is your tube Single and alone?Bring it to us!
For the LOW price of $4.00 your tube can be SEQUENCED and finally find a home!
*full plates too!*free pick-up service*data delivered 4-5pm
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Other equipment and services
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http://research.ncsu.edu/gsl