introduction to sperm hy-liter tm tests for forensic laboratories

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SPERM HY LITER Introduction to SPERM HY-LITER TM Tests for Forensic Laboratories Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence SPERM HY-LITER TM SPERM HY-LITER PLUS TM

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Introduction to SPERM HY-LITER TM Tests for Forensic Laboratories. Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence. SPERM HY-LITER TM. SPERM HY-LITER PLUS TM. SPERM. -. HY. LITER. SPERM HY-LITER TM. - PowerPoint PPT Presentation

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Page 1: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

Introduction to SPERM HY-LITERTM

Tests for Forensic Laboratories

Monoclonal Antibody-Based Fluorescent Detection of Human Sperm

from Sexual Assault Evidence

SPERM HY-LITERTM

SPERM HY-LITER PLUSTM

Page 2: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

SPERM HY-LITERTMSPERM

HY LITER- Test Format: Microscopic Screening Method for Sperm

Antigen Detected: Human Sperm Heads

Antibodies: Mouse monoclonal

Labeling: Sperm: Alexa 488 / Nuclei: DAPI

Sensitivity: Single Sperm Head

Cross-reaction: None detected

Test Read-out: Visual

Preparation Time: 120 minutes – 10 minutes ‘hands-on’

Page 3: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

SPERM HY-LITERTMSPERM

HY LITER-

SPERM HY-LITERTM staining

Sensitivity & SpecificityBuccal, Urine & Blood Extracts

Down to a Single Sperm

Phase Contrast

DAPI Filter FITC Filter

Page 4: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

SPERM

HY LITER-

SPERM HY-LITERTM staining

Species SpecificityAnimal Semen (various) without Human Semen

Phase Contrast

DAPI Filter FITC Filter

SPERM HY-LITER PLUSTM

Page 5: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

SPERM HY-LITERTMSPERM

HY LITER-

SPERM HY-LITERTM staining

Species SpecificityAnimal Semen (various)

with Human Semen

Phase Contrast

DAPI Filter FITC Filter

Page 6: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

SPERM HY-LITERTMSPERM

HY LITER-

First Positive Identification for Human Sperm

First Specific Identification for Human Sperm

Sensitivity down to a single sperm

Fully Compatible with Automated Methods

SPERM HY-LITER PLUSTM

Scan slides at lower magnification: 40X

Page 7: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

Jennifer Old, Ph.D.

Brett Schweers, Ph.D.

P.W. Boonlayangoor, Ph.D. Chicago

Liz Graffy, MS

Marisa Farhner, M.S

Acknowledgements:

SPERM HY-LITER PLUSTM

SPERM HY-LITERTM

Page 8: Introduction to  SPERM  HY-LITER TM Tests for Forensic Laboratories

SPERM

HY LITER

Questions

Comments Samples Gripes

Karl Reich

Tel 708.234.1200Fax 708.978.5115

[email protected]