introduction to microbiology - ksu...
TRANSCRIPT
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Introduction to Microbiology
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Laboratory Safety
• Don’t eat or drink in the lab
• Always wear lab coat and gloves inside
the lab
• Cover any minor cuts on your body
• Personal stuff will not be allowed on the
lab bench top
• You must wear shoes not sandals
• Long hair should be tied back
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• Report any accident to your supervisor
• Never take any culture out of the lab
• Never mouth-pipette
• Clean your bench after work
• Always wash your hands before leaving
the lab.
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Definitions
• Microbiology
It is a branch of biology that studies
microorganisms and their effects on humans
• Microorganisms
a collection of organisms that share the
characteristic of being visible only with a
microscope
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Microorganisms
include: Bacteria,
Viruses, Fungi, and
Parasites
They found
everywhere, Ex: in
the air, soil, water, in
& on our bodies
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Common Items Used in Microbiology Lab
• Bunsen burner
(flame):
Used to provide area
almost free of organism.
• Bacti-burner:
Same as Bunsen burner
but it is electrical.
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• Loops:
Used to o transfer organism from one place to
another (from culture to the slide).
A- Wire loop: large amount.
B- Straight loop: small amount
The loops are made up of special material
called Nichrome, it heats and get cool rapidly.
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• Slide:
Used to place the organism on top of it to examine it
under the microscope.
A- Plain slide.
B- Frosted end slide.
• Cover slip:
Used to cover the stained slide
to protect it from scratching,
contamination, and save it for a long time.
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Pipettes: Used to transfer solution from one place to
another.
• Graduated pipette: to transfer specific amount of solution. (1ml, 5ml, 10ml) Use Bi-pump with it.
• Pasteur pipette: to transfer non specific amount of solution.
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Container: Used to collect sample from the patient.
It should be sterile.
• Universal container:
(25 ml) used to collect large amount of sample, like: urine.
• Bijou bottle:
(5 ml) used to collect small amount of sample, like: CFS, gland fluid
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Petri dish:
Use it to put media inside it for culturing.
It should be sterile.
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• Disinfectant jar:
Used for the disposal of some used items, as:
Slides, cover slips, pipettes.
Disinfectant:
It is a chemical substance that kill or inhibit the
growth of micro organisms. Ex: Dettol, Clorox
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Equipments in Microbiology Lab
• Incubator: is a device used to grow and
maintain microbiological cultures (temperature
37º C)
• Autoclave: is a device used for sterilization at
temperature 121ºC, under pressure 15 atm for
15-20 min.
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Incubator Autoclave
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Saline
• It is a solution composed of 0.9% NaCl dissolved
in H2O (0.9 g NaCl dissolved in 100 ml water)
• Used to keep the intact shape of bacterial cell by
regulating the osmotic pressure
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Disposal of used items
• Trash bag: for disposal of non infectious material.
(Tissues, covering plastic).
• Disinfectant jar: for disposal of used pipettes,
slides & cover slips.
• Autoclave bag (Made of material that tolerate
high temperature): for disposal of plastic items
• Contaminated glass wears and flasks (reusable
items) must be sterilized by autoclave
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• Autoclave bag