introduction to microbes and infectious disease most microbes do not cause disease (what’s the...
DESCRIPTION
Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?) Microbes cause different diseases (why?) How do microbes get into the body (portals of entry) and spread (portals of exit)? . - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/1.jpg)
Introduction to microbes and infectious disease
Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulencefactor?)
Microbes cause different diseases (why?)
How do microbes get into the body (portals ofentry) and spread (portals of exit)?
![Page 2: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/2.jpg)
Why do some organisms cause disease whileothers do not?
Pathogens cause diseaseevade immune systemcause tissue damage
Other organisms harmlessly colonize the body
(Ch. 22-28; why do organisms cause disease ina particular part of the body?)
![Page 3: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/3.jpg)
p. 460
![Page 4: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/4.jpg)
p. 461; locations for normal flora
Resident?Transient?
![Page 5: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/5.jpg)
Pathogenesis of infectious disease
How does an organism cause disease?(impairment of function)
What is the course of disease?How is disease spread?
How is the organism shown to be pathogenic?
Is it always pathogenic (primary pathogen) oronly is susceptible people (opportunistic)
How virulent is the infectious organism?
![Page 6: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/6.jpg)
Contagious (communicable) diseases are easilyspread
Sometimes the infectious dose is very low (therefore easier to spread)
Example: dose is high for Salmonellalow for Shigella (both cause diarrhea)
Infectious dose can be calculated
![Page 7: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/7.jpg)
General sequence of events in infectiousdisease
p. 463Infection may be localized or systemicTime course can be short or long
![Page 8: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/8.jpg)
Koch’s postulates- classical way to determineif a particular organism causes disease
1. Microbe is present in all affected individuals2. Organism is isolated from a sick individual3. When organism is introduced into new hosts,
they get sick, too- and same organism is isolated from them.
Not all organism can be grown in pure cultureor tested
Postulates are applied on molecular level(what microbial product causes disease)
![Page 9: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/9.jpg)
Mechanisms of pathogenicity
Toxin production (endotoxin or exotoxin)toxins can be produced in body oringested
Colonization (usually GI or respiratory tract)acquire food, overcome normal flora
Invasion of tissues (avoid immune system)
Or some combination!
![Page 10: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/10.jpg)
How do bacteria establish infections?
Adhesion (adherence) is often a necessaryfirst step
Adhesins bind to specific molecules on cellsurface- contributes to tissue specificity
i.e., E.coli in urinary tract; Neisseria in repro-ductive tract
![Page 11: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/11.jpg)
How do microbes invade body
Skin- through lesions or vectors (bites)
Many bacteria have developed ways to getthrough innate immune barriers or bycrossing epithelia (cells specialized forengulfment and/or transport)
Some bacteria hide inside host cells(e.g., Listeria)
![Page 12: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/12.jpg)
Avoiding immune mechanisms
Gram-positive organisms are not sensitive tocomplement-mediated lysis
Some Gram-negatives use complement receptors or special glycosylation
Avoiding phagocytosiscapsules; complement inactivation
streptococci, staphylococci
![Page 13: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/13.jpg)
Surviving phagocytosisListeria, Shigella get out of phagosome
Salmonella- prevent fusion with lysosome
IgA proteases
Antigenic variation; mimicking host molecules(streptococci good at this)
![Page 14: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/14.jpg)
Pathogenic mechanisms
• Toxins– “A-B” (“B” attaches to cell (specificity); “A” part is the toxin– Cytotoxins damage cells membranes– Superantigens aggravate immune
response– Other damage specific tissues
![Page 15: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/15.jpg)
How do toxins damage host? (see p. 473)
A-B toxins: examples
I. Neurotoxins- interfere with transmission of nervous signal
C. botulinum- prevents release of acetylcholine(flaccid)
C. tetani- blocks inhibitory neuronsspastic
![Page 16: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/16.jpg)
II. Enterotoxins- oversecretion of fluids intointestine- E. coli; V. cholerae
III. Cytotoxins
B. anthracis, B. pertussis- oversecretion
C. diphtheriae, E. coli O157:H7, S. dysenteriaeinhibit protein synthesis
![Page 17: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/17.jpg)
Membrane-damaging toxins have differentstructure, directly attacking cell membranes
(Have different structure than A-B toxins)
Example: hemolytic bacteria (can lyse other membranes beside RBC membranes)
![Page 18: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/18.jpg)
Superantigens break the rules of antigenspecificity (also act differently than otherexotoxins)
Recall that T cells recognize antigen “presented”to them by MHC Class II on APCs (see p. 475)
Superantigens bind differently; can activatemay different T cells simultaneously
Excess stimulation can cause nausea, vomitingand sometimes shock
S. aureus toxin is well characterized (TSS)
![Page 19: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/19.jpg)
Most exotoxins are not heat-stable (exceptS. aureus superantigen)
Many exotoxins have been isolated and areused (as toxoids) for vaccines
See p. 477 for comparison with endotoxin
![Page 20: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/20.jpg)
Endotoxin is actually component of Gram-negatives (LPS), so cannot be isolatedlike an exotoxin can
Damage is due to inflammatory response to itcan be fatal (septic shock)
Heat-stable; not autoclavable
Limulus amoebocyte assay is used to test forendotoxins
![Page 21: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/21.jpg)
Thus immune response itself can contributeto disease
Inflammation
Hypersensitivity
Autoimmune disease (cross-reactive antibodies)
“Immune complex disease” can lead to kidneydamage
![Page 22: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/22.jpg)
Viral pathogenesis
All viruses must live within cell; some can causechronic or latent infections
Viruses bind to specific receptors on cells and(in animals) are endocytosed
May stick to one type of tissue or spread
![Page 23: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/23.jpg)
Many viruses have evolved mechanisms thatneutralize specific immune functions
Influenza, HIV- avoid interferons(HIV-infects regulatory T cells!)
Block MHC Class I expression
Form syncytia (cells fuse together)
Antigenic variation
![Page 24: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/24.jpg)
How do viruses damage cells?
Burst cellsApoptosis (avoids inflammatory response, too)Inflammatory response
(Most, but not, rashes associated with infectiousdisease are caused by viruses)
![Page 25: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/25.jpg)
Fungal infections
Remember how fungi eat!
Fungi can damage living tissue, too
Effect is strong in immunocompromisedpatients
Toxins
Hypersensitivities
![Page 26: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/26.jpg)
Helminths and protozoa
Depends on organism and host tissue
Malnutrition
Damage to colonized tissue; dysfunctionassociated with that
Can suppress immune response
![Page 27: Introduction to microbes and infectious disease Most microbes do NOT cause disease (what’s the difference, i.e., what is a virulence factor?)](https://reader035.vdocuments.us/reader035/viewer/2022062222/568167f4550346895ddd6c22/html5/thumbnails/27.jpg)
Study of pathogenesis involves:
Identification of virulence factors
Understanding host range of organisms
Normal host response to organism and howpathogen deals with it
Strategies for prevention and treatment