Introduction to Introduction to Light and Light and Electron Electron MicroscopyMicroscopy

NEU259 2012NEU259 2012

Gina Sosinsky, Ph. D.Gina Sosinsky, Ph. D.

Mark Ellisman, Ph. D.Mark Ellisman, Ph. D.

Class InfoClass Info

Tues-Thurs 10:00-11:00 am lectureTues-Thurs 10:00-11:00 am lecture NCMIR conference roomNCMIR conference room

Thursday 11-1 pm demonstrationsThursday 11-1 pm demonstrations Usually at NCMIR but may be elsewhere on Usually at NCMIR but may be elsewhere on campuscampus

Class limited to ~20 students totalClass limited to ~20 students total Preference is given to enrolled studentsPreference is given to enrolled students

Syllabus and lectures will be posted on:Syllabus and lectures will be posted on: http://ncmir.ucsd.edu/training/ucsd_courses/index.shtmhttp://ncmir.ucsd.edu/training/ucsd_courses/index.shtm http://cryoem.ucsd.edu/courses.shtm. http://cryoem.ucsd.edu/courses.shtm.

CourseworkCoursework Midterm (in class)Midterm (in class) Final (take home)Final (take home) Student presentations (last class, extra Student presentations (last class, extra credit)credit) My thesis project and how microscopy will be usedMy thesis project and how microscopy will be used

Any readings will be made available in class, Any readings will be made available in class, via email or from Jennifer Santos in our admin via email or from Jennifer Santos in our admin office.office.

Grades: Pass/FailGrades: Pass/Fail No letter grade will be given for this class; we No letter grade will be given for this class; we can file documentation to the department indicating can file documentation to the department indicating the grade that would have been given.the grade that would have been given.

Check with the department if you need a letter grade before Check with the department if you need a letter grade before taking the class to see if they will accept a letter from taking the class to see if they will accept a letter from the instructorsthe instructors

Workshop in Light and Workshop in Light and Electron Microscopy (Lab Electron Microscopy (Lab

Class)Class) NEU260NEU260

Practical course in light and electron microscopyPractical course in light and electron microscopy Hands on training in confocal, multiphoton, Hands on training in confocal, multiphoton, specimen preparation, TEM, electron tomography, specimen preparation, TEM, electron tomography, cryo-electron microscopycryo-electron microscopy

http://ncmir.ucsd.edu/Training/UCSDCourses/current.htmhttp://ncmir.ucsd.edu/Training/UCSDCourses/current.htm Tues-Thurs 1-3 pm*Tues-Thurs 1-3 pm*

May require extra time during student projects & May require extra time during student projects & training.training.

NEU259 is pre- or co-requisite for practical NEU259 is pre- or co-requisite for practical coursecourse

Offered through Interfaces Training ProgramOffered through Interfaces Training Program

Interfaces Training Interfaces Training ProgramProgram

The Interfaces Training Program is an interdisciplinary graduate training program at the interfaces between the Biological, Medical, Physical and Engineering Sciences. Courses are all lab courses.

UCSD was one of ten universities selected through the Howard Hughes Medical Institute (HHMI) to initiate this new program. There is now a NIH NIBIB training grant for the program and UCSD support for the lab courses.

CoursesCoursesLogo designed by Interfaces student(s).

Program GoalsProgram Goals

1) Focused Collaborationacross nine graduate degree programs at UCSD to train a new generation of cross-disciplinary scientist.

2) State-of-the-art Interdisciplinary Trainingthrough a new technology-centered hands-on graduate laboratory course curriculum.

3) Novel Emphasis on Researchaimed at integrative and quantitative analysis across multiple scales of biological organization from molecule to organism in health and disease.

ParticipatingPrograms / Departments

Department of BioengineeringDivision of Biological SciencesBioinformatics Graduate ProgramBiomedical Sciences Graduate ProgramDepartment of Chemistry and BiochemistryDepartment of MathematicsMolecular Pathology Ph.D. ProgramNeurosciences Graduate Program Department of PhysicsDepartment of Radiology

ParticipatingFaculty

• 25 outstanding faculty members• Appointed representatives

from all participating degree programs plus the Radiology Department

• Core leaders and Co-instructorsfor six new graduate laboratory courses

• Includes three investigators of theHoward Hughes Medical Institute

• Two faculty with appointments at the neighboring Salk and Burnham Institutes

Application and Admission Students interested in participating must:

1) First, be admitted through one of the participating doctoral programs and/or departments: Bioengineering,

Bioinformatics, Biology, Biomedical Sciences, Chemistry and Biochemistry, Mathematics, Neuroscience, Molecular Pathology, and Physics.

2) Then, join a Student Affiliate by completing a Student Affiliate Application. Send e- Send e-mail to Irene Jacobo (mail to Irene Jacobo (ihjacobo@ucsd.edu)

Student Affiliates

Student Affiliates will participate in:

Interfaces Course Enrollment

Funding through Teaching Assistantships

Multi-Scale Biology Specialization

Special Events

Funding through Formal Traineeships

Curriculum and Training

Curriculum Includes• Six hands-on graduate laboratory courses taught by

interdisciplinary teams of faculty members

Labs will introduce students to• state-of-the-art techniques studying living systems

across physical scales from molecules to the whole organism

• multi-scale techniques for measuring, imaging, manipulating and analyzing living systems

Labs are designed• to train students for modern research opportunities

in integrative multi-scale biomedical science